Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	458,470	G→T	E349* (GAA→TAA)	clpX →	ATP‑dependent Clp protease ATP‑binding subunit ClpX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	458,470	0	G	T	100.0%	266.9 / NA	77	E349* (GAA→TAA)	clpX	ATP‑dependent Clp protease ATP‑binding subunit ClpX
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (48/29);  total (48/29)

TATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATACCATGTACGATCTGCCGTCCATGGAAGA  >  NC_000913/458337‑458600                                                                                                                                      |                                                                                                                                   tATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTcc                                                                                                                              >  2:996994/1‑140 (MQ=255) tATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTcc                                                                                                                              >  2:457016/1‑140 (MQ=255)  atttgtcgtcTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCg                                                                                                                             >  1:398959/5‑140 (MQ=255)  aTTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCg                                                                                                                             >  2:866145/1‑140 (MQ=255)       tcgtcTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACg                                                                                                                        <  2:649643/140‑1 (MQ=255)        cgtcTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCTTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGa                                                                                                                       <  1:1165163/140‑1 (MQ=255)        cgtcTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGa                                                                                                                       >  2:1161373/1‑140 (MQ=255)         gtcTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAg                                                                                                                      >  2:1124667/1‑140 (MQ=255)            tGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGgcg                                                                                                                   <  2:1209537/140‑1 (MQ=255)                                aatgaaCTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGaa                                                                                               >  2:292333/1‑140 (MQ=255)                                     aCTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGa                                                                                          >  1:360149/1‑140 (MQ=255)                                      cTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGAt                                                                                         >  2:445302/1‑140 (MQ=255)                                        gAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATgg                                                                                       >  2:870612/1‑140 (MQ=255)                                         aGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGgc                                                                                      >  1:1044180/1‑140 (MQ=255)                                          gCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGgcg                                                                                     >  1:139921/1‑140 (MQ=255)                                              agaagCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTa                                                                                 >  2:610344/1‑140 (MQ=255)                                              agaagCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTa                                                                                 >  1:154087/1‑140 (MQ=255)                                                  gCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAAc                                                                             <  1:610347/140‑1 (MQ=255)                                                   ctctGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAAcc                                                                            >  1:649751/1‑140 (MQ=255)                                                        aTTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGc                                                                       >  1:639131/1‑140 (MQ=255)                                                             gATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTg                                                                  >  2:728137/1‑140 (MQ=255)                                                              aTCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGccc                                                                     <  2:611971/136‑1 (MQ=255)                                                              aTCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGccc                                                                     >  1:611971/1‑136 (MQ=255)                                                              aTCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTgg                                                                 >  2:369387/1‑140 (MQ=255)                                                              aTCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTgg                                                                 >  1:1108594/1‑140 (MQ=255)                                                               tCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGc                                                                <  1:243475/140‑1 (MQ=255)                                                               tCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGc                                                                <  2:576981/140‑1 (MQ=255)                                                                  tCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTg                                                             >  1:640892/1‑140 (MQ=255)                                                                  tCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTg                                                             <  1:369387/140‑1 (MQ=255)                                                                  tCAAAGAGCCGAAAAACGCACTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTg                                                             >  1:667308/1‑140 (MQ=255)                                                                   cAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGc                                                            >  1:514580/1‑140 (MQ=255)                                                                    aaaGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCg                                                           >  2:78388/1‑140 (MQ=255)                                                                    aaaGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCg                                                           <  2:398959/140‑1 (MQ=255)                                                                      agagCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGtt                                                         >  1:947780/1‑140 (MQ=255)                                                                          ccGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCAt                                                     >  1:710857/1‑140 (MQ=255)                                                                          ccGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCAt                                                     >  2:262104/1‑140 (MQ=255)                                                                          ccGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCAt                                                     >  2:317082/1‑140 (MQ=255)                                                                          ccGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCAt                                                     >  2:402410/1‑140 (MQ=255)                                                                          ccGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCAt                                                     >  2:73893/1‑140 (MQ=255)                                                                          ccGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCAt                                                     >  2:9288/1‑140 (MQ=255)                                                                          ccGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCAGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCAt                                                     >  2:849612/1‑140 (MQ=255)                                                                           cGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATc                                                    >  2:75383/1‑140 (MQ=255)                                                                            gAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCg                                                   >  1:98228/1‑140 (MQ=255)                                                                             aaaaaCGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGt                                                  >  2:463880/1‑140 (MQ=255)                                                                             aaaaaCGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGt                                                  <  1:1124667/140‑1 (MQ=255)                                                                                       tGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCa                                        <  1:73893/140‑1 (MQ=255)                                                                                       tGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCa                                        <  1:78388/140‑1 (MQ=255)                                                                                       tGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCa                                        <  2:840540/140‑1 (MQ=255)                                                                                       tGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCa                                        <  1:866145/140‑1 (MQ=255)                                                                                       tGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCa                                        <  2:1104453/140‑1 (MQ=255)                                                                                            aaGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCt                                   <  2:154087/140‑1 (MQ=255)                                                                                            aaGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCt                                   <  1:445302/140‑1 (MQ=255)                                                                                              gCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCg                                 <  1:9288/140‑1 (MQ=255)                                                                                                aGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTTCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGAt                               <  2:189498/140‑1 (MQ=255)                                                                                                aGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGAt                               <  1:75383/140‑1 (MQ=255)                                                                                                aGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGAt                               <  1:996994/140‑1 (MQ=255)                                                                                                 gTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATa                              <  1:317082/140‑1 (MQ=255)                                                                                                  tATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATAc                             <  1:457016/140‑1 (MQ=255)                                                                                                   aTCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATAcc                            >  1:688029/1‑140 (MQ=255)                                                                                                    tCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCt                                   <  1:862326/132‑1 (MQ=255)                                                                                                    tCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCt                                   >  2:862326/1‑132 (MQ=255)                                                                                                     cAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATACCAt                          <  2:139921/140‑1 (MQ=255)                                                                                                     cAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATACCAt                          <  1:463880/140‑1 (MQ=255)                                                                                                        gcgcTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATACCATGTa                       >  2:45751/1‑140 (MQ=255)                                                                                                         cgcTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATAcc                            >  1:374606/1‑134 (MQ=255)                                                                                                         cgcTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATAcc                            <  2:374606/134‑1 (MQ=255)                                                                                                         cgcTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATACCATGTAc                      <  2:1044180/140‑1 (MQ=255)                                                                                                         cgcTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATACCATGTAc                      >  1:831705/1‑140 (MQ=255)                                                                                                         cgcTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATACCATGTAc                      >  2:1026540/1‑140 (MQ=255)                                                                                                          gcTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATACCATGTACg                     >  1:534575/1‑140 (MQ=255)                                                                                                           cTGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATACCATGTACGa                    >  2:516687/1‑140 (MQ=255)                                                                                                            tGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATACCATGTACGAt                   >  2:1169495/1‑140 (MQ=255)                                                                                                            tGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATACCATGTACGAt                   >  2:810912/1‑140 (MQ=255)                                                                                                            tGTTTAATCTGGAAGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATACCATGTACGAt                   >  1:339541/1‑140 (MQ=255)                                                                                                                        aaGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATACCATGTACGATCTGCCGTCCATg       >  1:232244/1‑140 (MQ=255)                                                                                                                        aaGGCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATACCATGTACGATCTGCCGTCCATg       >  2:624186/1‑140 (MQ=255)                                                                                                                           gCGTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATACCATGTACGATCTGCCGTCCATGGaa    <  2:947780/140‑1 (MQ=255)                                                                                                                             gTGGATCTGTAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATACCATGTACGATCTGCCGTCCATGGAAGa  >  2:90864/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                   TATTGGTCGTCTGCCGGTTGTCGCAACGTTGAATGAACTGAGCGAAGAAGCTCTGATTCAGATCCTCAAAGAGCCGAAAAACGCCCTGACCAAGCAGTATCAGGCGCTGTTTAATCTGGAAGGCGTGGATCTGGAATTCCGTGACGAGGCGCTGGATGCTATCGCTAAGAAAGCGATGGCGCGTAAAACCGGTGCCCGTGGCCTGCGTTCCATCGTAGAAGCCGCACTGCTCGATACCATGTACGATCTGCCGTCCATGGAAGA  >  NC_000913/458337‑458600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑