Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,729,171	G→A	D67D (GAC→GAT)	xylB ←	xylulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,729,171	0	G	A	100.0%	198.9 / NA	56	D67D (GAC→GAT)	xylB	xylulokinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/35);  total (21/35)

AACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGTAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAA  >  NC_000913/3729036‑3729301                                                                                                                                        |                                                                                                                                   aaCTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGTAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCt                                                                                                                                <  1:175533/140‑1 (MQ=255)    tCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGTAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCa                                                                                                                             <  2:232252/140‑1 (MQ=255)     cgcgCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGTAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCag                                                                                                                            <  2:1056068/140‑1 (MQ=255)     cgcgCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGTAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCag                                                                                                                            >  1:993070/1‑140 (MQ=255)     cgcgCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGTAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCag                                                                                                                            <  1:670855/140‑1 (MQ=255)              aGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGTAACACCCGTTGCTGAGCATCCAGCCAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAACGCTTTAACATCCTGCAGAGAATGCTg                                                                                                                   >  2:914728/1‑140 (MQ=255)                     tGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGTAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGccc                                                                                                            <  1:553562/140‑1 (MQ=255)                         ctctTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGTAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCagag                                                                                                        <  1:1178742/140‑1 (MQ=255)                             tGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGTAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCttt                                                                                                    <  1:805517/140‑1 (MQ=255)                               cgcACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGTAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCag                                                                                                          >  1:249952/1‑132 (MQ=255)                               cgcACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGTAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCag                                                                                                          <  2:249952/132‑1 (MQ=255)                               cgcACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGTAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCa                                                                                                  >  2:797637/1‑140 (MQ=255)                               cgcACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGTAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCa                                                                                                  >  2:684583/1‑140 (MQ=255)                               cgcACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGTAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCa                                                                                                  >  2:58531/1‑140 (MQ=255)                                gcACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGTAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCAt                                                                                                 <  1:976729/140‑1 (MQ=255)                                   caGCGCCCGTCGTTCCACAAAATGGCAGGGCGTAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTgc                                                                                              <  1:1149147/140‑1 (MQ=255)                                    aGCGCCCGTCGTTCCACAAAATGGCAGGGCGTAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTgcg                                                                                             >  1:158539/1‑140 (MQ=255)                                    aGCGCCCGTCGTTCCACAAAATGGCAGGGCGTAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTgcg                                                                                             >  2:865615/1‑140 (MQ=255)                                     gcgcCCGTCGTTCCACAAAATGGCAGGGCGTAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTgcgc                                                                                            <  2:526741/140‑1 (MQ=255)                                      cgcCCGTCGTTCCACAAAATGGCAGGGCGTAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGcgcg                                                                                           >  2:586453/1‑140 (MQ=255)                                      cgcCCGTCGTTCCACAAAATGGCAGGGCGTAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGcgcg                                                                                           >  1:488523/1‑140 (MQ=255)                                      cgcCCGTCGTTCCACAAAATGGCAGGGCGTAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGcgcg                                                                                           >  2:884742/1‑140 (MQ=255)                                         ccGTCGTTCCACAAAATGGCAGGGCGTAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATc                                                                                        >  1:239571/1‑140 (MQ=255)                                             cgtTTCACAAAATGGCAGGGCGTAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCT‑GCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTtg                                                                                   <  1:995080/140‑1 (MQ=255)                                              gtTCCACAAAATGGCAGGGCGTAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTtg                                                                                   <  2:555292/140‑1 (MQ=255)                                              gtTCCACAAAATGGCAGGGCGTAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTtg                                                                                   <  1:661512/140‑1 (MQ=255)                                              gtTCCACAAAATGGCAGGGCGTAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTtg                                                                                   <  2:239571/140‑1 (MQ=255)                                                   acaAAATGGCAGGGCGTAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTtgcctgc                                                                              <  2:443086/140‑1 (MQ=255)                                                   acaAAATGGCAGGGCGTAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTtgcctgc                                                                              <  1:914728/140‑1 (MQ=255)                                                   acaAAATGGCAGGGCGTAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATACTGATCGCCCAGAGCTTTCATTGCGCGATCAGTtgcctgc                                                                              >  2:1018409/1‑140 (MQ=255)                                                               ggCGTAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTcc                                                                  >  1:677840/1‑140 (MQ=255)                                                               ggCGTAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTcc                                                                  <  1:733636/140‑1 (MQ=255)                                                               ggCGTAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTcc                                                                  <  1:797637/140‑1 (MQ=255)                                                               ggCGTAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTcc                                                                  <  2:1029817/140‑1 (MQ=255)                                                                 cGTAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCgg                                                                >  1:1024058/1‑140 (MQ=255)                                                                         ccGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGtt                                                        <  1:1074035/140‑1 (MQ=255)                                                                           ggtGCCGAGCAACCAGCAAAGGTGCTCCGTGCCTCTGGGCGGCCATAACCAATGCCTTTACAACCTGCAGGGAATTCCGATCGCCCAGAGCTTTCATTTCGCGATCAGGTTCCTTCCACCACTGGTCCGGGGCTTGTTcc                                                      >  2:242053/3‑140 (MQ=255)                                                                           gTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTcc                                                      >  1:28241/1‑140 (MQ=255)                                                                             tGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGa                                                    >  2:770524/1‑140 (MQ=255)                                                                              gCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGAc                                                   <  2:993070/140‑1 (MQ=255)                                                                              gCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGAc                                                   >  2:894415/1‑140 (MQ=255)                                                                              gCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGAc                                                   <  1:879423/140‑1 (MQ=255)                                                                              gCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGAc                                                   <  1:827029/140‑1 (MQ=255)                                                                              gCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATACTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGAc                                                   <  1:1018409/140‑1 (MQ=255)                                                                                   gCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCagag                                              <  2:1131219/140‑1 (MQ=255)                                                                                     aTCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTg                                            >  2:174926/1‑140 (MQ=255)                                                                                         aGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATg                                        <  1:713530/140‑1 (MQ=255)                                                                                                   cTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGaa                              >  1:564483/1‑140 (MQ=255)                                                                                                        tGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGt                         <  2:564483/140‑1 (MQ=255)                                                                                                        tGCATCTGGCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGt                         <  2:320545/140‑1 (MQ=255)                                                                                                                gCCGGCAATACCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCtttt                 >  1:664987/1‑140 (MQ=255)                                                                                                                         aCCCAATGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTgcg        <  1:174926/140‑1 (MQ=255)                                                                                                                           ccAATGCTTTAACATCCTGCAGAGAACGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCgca      <  1:917515/140‑1 (MQ=255)                                                                                                                               tGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCaa  <  1:633347/140‑1 (MQ=255)                                                                                                                               tGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCaa  <  2:28241/140‑1 (MQ=255)                                                                                                                               tGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCaa  <  1:244147/140‑1 (MQ=255)                                                                                                                               tGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCaa  <  1:894415/140‑1 (MQ=255)                                                                                                                               tGCTTTAACATCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCaa  >  2:1114682/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                   AACTCGCGCTTCCAGCAAAGTGCACTCTTGCGCACAGCGCCCGTCGTTCCACAAAATGGCAGGGCGTAACACCCGTTGCTGAGCATCCAGCAAGGTTGCTCCGTGCATCTGGCCGGCAATACCCAATGCTTTAACGTCCTGCAGAGAATGCTGATCGCCCAGAGCTTTCATTGCGCGATCAGTTGCCTGCCACCACTGTTCCGGGTCTTGTTCCGACCAGAGTGGATGCGGGCGCGAAACGGTCAGCTTTTCCGTTTGCGCAGCAA  >  NC_000913/3729036‑3729301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑