Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	624,859	G→T	intergenic (‑349/‑26)	fepB ← / → entC	ferric enterobactin ABC transporter periplasmic binding protein/isochorismate synthase EntC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	624,859	0	G	T	100.0%	204.6 / NA	58	intergenic (‑349/‑26)	fepB/entC	ferric enterobactin ABC transporter periplasmic binding protein/isochorismate synthase EntC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (19/39);  total (19/39)

TGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAAGCCTTTATCATTTTGTGGAGGATGATATGGATACGTCACTGGCTGAGGAAGTACAGCAGACCATGGCAACACTTGCGCCCAATCGCTTTTTCTTTATGTCGCCGTACCGCAGTTTTACGACGTCAGGATGTTT  >  NC_000913/624727‑624991                                                                                                                                     |                                                                                                                                     tGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTAt                                                                                                                               >  1:357679/1‑140 (MQ=255)     tctcATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCAtt                                                                                                                           >  2:1179101/1‑140 (MQ=255)     tctcATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCAtt                                                                                                                           >  2:186902/1‑140 (MQ=255)      ctcATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCAttt                                                                                                                          >  1:861056/1‑140 (MQ=255)      ctcATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCAttt                                                                                                                          >  2:168974/1‑140 (MQ=255)        cATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTtg                                                                                                                        <  1:118538/140‑1 (MQ=255)          ttttCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTtgtg                                                                                                                      <  1:1180009/140‑1 (MQ=255)            ttCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTgga                                                                                                                    >  1:907021/1‑140 (MQ=255)             tCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTggag                                                                                                                   <  2:217990/140‑1 (MQ=255)              cATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTggagg                                                                                                                  <  1:427448/140‑1 (MQ=255)              cATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTggagg                                                                                                                  <  2:122876/140‑1 (MQ=255)              cATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTggagg                                                                                                                  <  2:907021/140‑1 (MQ=255)              cATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTATGTggagg                                                                                                                  <  2:790377/140‑1 (MQ=255)              cATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCGTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTACTAAATCCTTTATCATTTTGTggagg                                                                                                                  <  2:860693/140‑1 (MQ=255)               aTTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTggagga                                                                                                                 <  2:160521/140‑1 (MQ=255)               aTTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTggagga                                                                                                                 <  1:1009031/140‑1 (MQ=255)                 tGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGgatg                                                                                                               <  2:240118/140‑1 (MQ=255)                    aaCCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGata                                                                                                            <  1:46826/140‑1 (MQ=255)                     aCCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGatat                                                                                                           >  2:66343/1‑140 (MQ=255)                     aCCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTCCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGatat                                                                                                           >  2:1186152/1‑140 (MQ=255)                         caACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGATATGGAt                                                                                                       <  1:1202800/140‑1 (MQ=255)                         caACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGATATGGAt                                                                                                       <  1:901565/140‑1 (MQ=255)                              aGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGATATGGATACGTc                                                                                                  <  1:1040327/140‑1 (MQ=255)                               gATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGATATGGATACGTCa                                                                                                 <  1:373250/140‑1 (MQ=255)                               gATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGATATGGATACGTCa                                                                                                 <  2:99777/140‑1 (MQ=255)                               gATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGATATGGATACGTCa                                                                                                 <  1:508140/140‑1 (MQ=255)                                aTGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGATATGGATACGTCAc                                                                                                >  2:1082450/1‑140 (MQ=255)                                 tGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGATATGGATACGTCACt                                                                                               <  1:1055798/140‑1 (MQ=255)                                      ccccGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGATATGGATACGTCACTg                                                                                              >  1:1119447/1‑136 (MQ=255)                                      ccccGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGATATGGATACGTCACTg                                                                                              <  2:1119447/136‑1 (MQ=255)                                      ccccGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGATATGGATACGTCACTGGCTg                                                                                          <  1:1158257/140‑1 (MQ=255)                                       cccGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGATATGGATACGTCACTGGCTGa                                                                                         <  2:861056/140‑1 (MQ=255)                                          gAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGATATGGATACGTCACTGGCTGAGGa                                                                                      >  1:53781/1‑140 (MQ=255)                                           aGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGATATGGATACGTCACTGGc                                                                                            >  2:1073841/1‑133 (MQ=255)                                           aGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGATATGGATACGTCACTGGc                                                                                            <  1:1073841/133‑1 (MQ=255)                                           aGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGATATGGATACGTCACTGGCTGAGGaa                                                                                     <  1:1080772/140‑1 (MQ=255)                                            gTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGATATGGATACGTCACTGGCTGAGGAAg                                                                                    <  1:513000/140‑1 (MQ=255)                                            gTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGATATGGATACGTCACTGGCTGAGGAAg                                                                                    <  2:84714/140‑1 (MQ=255)                                              tGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGATATGGATACGTCACTGGCTGAGGAAGTa                                                                                  >  1:290064/1‑140 (MQ=255)                                                   aTTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGATATGGATACGTCACTGGCTGAGGAAGTAcagca                                                                             <  1:241967/140‑1 (MQ=255)                                                         ttACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGATATGGATACGTCACTGGCTGAGGAAGTACAGCAGACCAt                                                                       <  2:562326/140‑1 (MQ=255)                                                            ccTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGATATGGATACGTCACTGGCTGAGGAAGTACAGCAGACCATGGc                                                                    <  2:290064/140‑1 (MQ=255)                                                            ccTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGATATGGATACGTCACTGGCTGAGGAAGTACAGCAGACCATGGc                                                                    >  2:35771/1‑140 (MQ=255)                                                             cTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGATATGGATACGTCACTGGCTGAGGAAGTACAGCAGACCATGGCa                                                                   <  1:1082450/140‑1 (MQ=255)                                                                      tGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGATATGGATACGTCACTGGCTGAGGAAGTACAGCAGACCATGGCAACACTTgcg                                                          >  2:1098604/1‑140 (MQ=255)                                                                       gACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGATATGGATACGTCACTGGCTGAGGAAGTACAGCAGACCATGGCAACACTTgcgc                                                         >  1:254394/1‑140 (MQ=255)                                                                            aGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGATATGGATACGTCACTGGCTGAGGAAGTACAGCAGACCATGGCAACACTTGCGCCCAAt                                                    <  1:35771/140‑1 (MQ=255)                                                                            aGTCCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGATATGGATACGTCACTGGCTGAGGAAGTACAGCAGACCATGGCAACACTTGCGCCCAAt                                                    <  1:1186152/140‑1 (MQ=255)                                                                               gcgcGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGATATGGATACGTCACTGGCTGAGGAAGTACAGCAGACCATGGCAACACTTGCGCCCAATCGc                                                 >  2:321318/1‑140 (MQ=255)                                                                                cgcgTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGATATGGATACGTCACTGGCTGAGGAAGTACAGCAGACCATGGCAACACTTGCGCCCAATCGCt                                                <  1:168974/140‑1 (MQ=255)                                                                                         ttttAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGATATGGATACGTCACTGGCTGAGGAAGTACAGCAGACCATGGCAACACTTGCGCCCAATCGCTTTTTCTTTa                                       <  2:53781/140‑1 (MQ=255)                                                                                                     gACCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGATATGGATACGTCACTGGCTGAGGAAGTACAGCAGACCATGGTAACACTTGCGCCCAATCGCTTTTTCTTTATGTCGCCGTAcc                           <  1:1098604/140‑1 (MQ=255)                                                                                                      aCCGAAAATATAAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGATATGGATACGTCACTGGCTGAGGAAGTACAGCAGACCATGGCAACACTTGCGCCCAATCGCTTTTTCTTTATGTCGCCGTACCg                          <  1:1179101/140‑1 (MQ=255)                                                                                                              tataAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGATATGGATACGTCACTGGCTGAGGAAGTACAGCAGACCATGGCAACACTTGCGCCCAATCGCTTTTTCTTTATGTCGCCGTACCGCAGTTTTa                  <  2:1185093/140‑1 (MQ=255)                                                                                                               ataAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGATATGGATACGTCACTGGCTGAGGAAGTACAGCAGACCATGGCAACACTTGCGCCCAATCGCTTTTTCTTTATGTCGCCGTACCGCAGTTTTac                 <  2:254394/140‑1 (MQ=255)                                                                                                                taAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGATATGGATACGTCACTGGCTGAGGAAGTACAGCAGACCATGGCAACACTTGCGCCCAATCGCTTTTTCTTTATGTCGCCGTACCGCAGTTTTacg                <  1:833971/140‑1 (MQ=255)                                                                                                                taAATGATAATCATTATTAAATCCTTTATCATTTTGTGGAGGATGATATGGATACGTCACTGGCTGAGGAAGTACAGCAGACCATGGCAACACTTGCGCCCAATCGCTTTTTCTTTATGTCGCCGTACCGCAGTTTTacg                <  1:429113/140‑1 (MQ=255)                                                                                                                        aaTCATTATTAAATCCTTTATCATTTTGTGGAGGATGATATGGATACGTCACTGGCTGAGGAAGTACAGCAGACCATGGCAACACTTGCGCCCAATCGCTTTTTCTTTATGTCGCCGTACCGCAGTTTTACGACGTCAgg        >  1:235682/1‑140 (MQ=255)                                                                                                                              tattaAATCCTTTATCATTTTGTGGAGGATGATATGGATACGTCACTGGCTGAGGAAGTACAGCAGACCATGGCAACACTTGCGCCCAATCGCTTTTTCTTTATGTCGCCGTACCGCAGTTTTACGACGTCAGGATGttt  >  2:1210611/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                     TGCTTCTCATTTTCATTGTAACCACAACCAGATGCAACCCCGAGTTGCAGATTGCGTTACCTCAAGAGTTGACATAGTGCGCGTTTGCTTTTAGGTTAGCGACCGAAAATATAAATGATAATCATTATTAAAGCCTTTATCATTTTGTGGAGGATGATATGGATACGTCACTGGCTGAGGAAGTACAGCAGACCATGGCAACACTTGCGCCCAATCGCTTTTTCTTTATGTCGCCGTACCGCAGTTTTACGACGTCAGGATGTTT  >  NC_000913/624727‑624991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑