Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	974,374	A→G	D19G (GAC→GGC)	mukF →	chromosome partitioning protein MukF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	974,374	0	A	G	100.0%	231.0 / NA	63	D19G (GAC→GGC)	mukF	chromosome partitioning protein MukF
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/29);  total (34/29)

TTATTGCCGTCAGGAACCGTATATTACCCTGGGGCGTTATATTCATGTCACCGCGCGCAAACCGCAGAGCAAGGATAAAGTATGAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGACTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCGAGCGTCTGGATGGTGAGATGAGTGAAGGCGAGCTGGTGGATGCATTCCGCCATGTGAGTGATGCGT  >  NC_000913/974238‑974505                                                                                                                                         |                                                                                                                                    ttATTGCCGTCAGGAACCGTATATTACCCTGGGGCGTTATATTCATGTCACCGCGCGCAAACCGCAGAGCAAGGATAAAGTATGAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCtt                                                                                                                                  >  1:1054878/1‑140 (MQ=255)        cGTCAGGAACCGTATATTACCCTGGGGCGTTATATTCATGTCACCGCGCGCAAACCGCAGAGCAAGGATAAAGTATGAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCAtc                                                                                                                           <  2:557745/140‑1 (MQ=255)                   gTATATTACCCTGGGGCGTTATATTCATGTCACCGCGCGCAAACCGCAGAGCAAGGATAAAGTATGAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGt                                                                                                                <  2:418000/140‑1 (MQ=255)                           cccTGGGGCGTTATATTCATGTCACCGCGCGCAAACCGCAGAGCAAGGATAAAGTATGAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGAc                                                                                                        >  1:276835/1‑140 (MQ=255)                           cccTGGGGCGTTATATTCATGTCACCGCGCGCAAACCGCAGAGCAAGGATAAAGTATGAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGAc                                                                                                        >  1:362311/1‑140 (MQ=255)                            ccTGGGGCGTTATATTCATGTCACCGCGCGCAAACCGCAGAGCAAGGATAAAGTATGAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGAct                                                                                                       >  1:521287/1‑140 (MQ=255)                            ccTGGGGCGTTATATTCATGTCACCGCGCGCAAACCGCAGAGCAAGGATAAAGTATGAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGAct                                                                                                       >  2:629936/1‑140 (MQ=255)                            ccTGGGGCGTTATATTCATGTCACCGCGCGCAAACCGCAGAGCAAGGATAAAGTATGAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGAct                                                                                                       >  2:31749/1‑140 (MQ=255)                            ccTGGGGCGTTATATTCATGTCACCGCGCGCAAACCGCAGAGCAAGGATAAAGTATGAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGAct                                                                                                       <  2:276835/140‑1 (MQ=255)                            ccTGGGGCGTTATATTCATGTCACCGCGCGCAAACCGCAGAGCAAGGATAAAGTATGAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGAct                                                                                                       >  2:130889/1‑140 (MQ=255)                             cTGGGGCGTTATATTCATGTCACCGCGCGCAAACCGCAGAGCAAGGATAAAGTATGAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGActc                                                                                                      >  1:984936/1‑140 (MQ=255)                               ggggCGTTATATTCATGTCACCGCGCGCAAACCGCAGAGCAAGGATAAAGTATGAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGActctc                                                                                                    >  1:958180/1‑140 (MQ=255)                                gggCGTTATATTCATGTCACCGCGCGCAAACCGCAGAGCAAGGATAAAGTATGAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGActctct                                                                                                   >  1:728264/1‑140 (MQ=255)                                 ggCGTTATATTCATGTCACCGCGCGCAAACCGCAGAGCAAGGATAAAGTATGAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCtt                                                                                                  >  1:1010409/1‑140 (MQ=255)                                     ttATATTCATGTCACCGCGCGCAAACCGCAGAGCAAGGATAAAGTATGAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCg                                                                                                          <  2:995816/128‑1 (MQ=255)                                     ttATATTCATGTCACCGCGCGCAAACCGCAGAGCAAGGATAAAGTATGAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCg                                                                                                          >  1:995816/1‑128 (MQ=255)                                     ttATATTCATGTCACCGCGCGCAAACCGCAGAGCAAGGATAAAGTATGAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTct                                                                                              >  1:322771/1‑140 (MQ=255)                                     ttATATTCATGTCACCGCGCGCAAACCGCAGAGCAAGGATAAAGTATGAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCGCTTTTct                                                                                              >  2:114869/1‑140 (MQ=255)                                                tCACCGCGCGCAAACCGCAGAGCAAGGATAAAGTATGAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTg                                                                                   <  1:191911/140‑1 (MQ=255)                                                  aCCGCGCGCAAACCGCAGAGCAAGGATAAAGTATGAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCggcttac                                                                                 >  2:1043711/1‑135 (MQ=255)                                                  aCCGCGCGCAAACCGCAGAGCAAGGATAAAGTATGAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGcc                                                                                 >  1:1031429/1‑140 (MQ=255)                                                  aCCGCGCGCAAACCGCAGAGCAAGGATAAAGTATGAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGcc                                                                                 <  1:1095216/140‑1 (MQ=255)                                                  aCCGCGCGCAAACCGCAGAGCAAGGATAAAGTATGAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGcc                                                                                 >  2:160718/1‑140 (MQ=255)                                                       gcgcAAACCGCAGAGCAAGGATAAAGTATGAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCt                                                                            >  1:209331/1‑140 (MQ=255)                                                          cAAACCGCAGAGCAAGGATAAAGTATGAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGaa                                                                         <  2:521287/140‑1 (MQ=255)                                                               cGCAGAGCAAGGATAAAGTATGAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCg                                                                    <  1:805476/140‑1 (MQ=255)                                                               cGCAGAGCAAGGATAAAGTATGAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCg                                                                    <  2:1031429/140‑1 (MQ=255)                                                                gCAGAGCAAGGATAAAGTATGAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCGa                                                                   <  2:592797/140‑1 (MQ=255)                                                                 cAGAGCAAGGATAAAGTATGAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCGAg                                                                  <  1:850692/140‑1 (MQ=255)                                                                 cAGAGCAAGGATAAAGTATGAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCGAg                                                                  <  1:507246/140‑1 (MQ=255)                                                                        aGGATAAAGTATGAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCGAGCGTCTgg                                                           <  2:323088/140‑1 (MQ=255)                                                                                gTATGAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCGAGCGTCTGGATGGTgag                                                   <  1:1077825/140‑1 (MQ=255)                                                                                gTATGAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCGAGCGTCTGGATGGTgag                                                   <  2:984936/140‑1 (MQ=255)                                                                                  aTGAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCGAGCGTCTGGATGGTGAGAt                                                 <  2:362311/140‑1 (MQ=255)                                                                                    gAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCGAGCGTCTgg                                                           <  2:410803/128‑1 (MQ=255)                                                                                    gAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCGAGCGTCTgg                                                           >  1:410803/1‑128 (MQ=255)                                                                                    gAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCGAGCGTCTGGATGGTGAGATGa                                               >  1:463251/1‑140 (MQ=255)                                                                                    gAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCGAGCGTCTGGATGGTGAGATGa                                               >  2:647802/1‑140 (MQ=255)                                                                                     aGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCGAGCGGCTGGGTGGTGAGATGAg                                              >  2:888486/1‑140 (MQ=255)                                                                                          tttttCCCAGACAGTTCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTTTCCATCTCGCTGGCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCGAGCGTCTGGATGGTGAGATGAGTGAAg                                         <  2:3220/139‑1 (MQ=255)                                                                                           ttttCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCCCGCTGAACGGCGGGCGTCTGGGTGGTGGGATGAGGGAAgg                                        >  2:230035/1‑140 (MQ=255)                                                                                           ttttCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCGAGCGTCTGGATGGTGAGATGAGTGAAgg                                        >  2:160648/1‑140 (MQ=255)                                                                                               cccAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCGAGCGTCTGGATGGTGAGATGAGTGAAGGCGAg                                    <  2:728264/140‑1 (MQ=255)                                                                                                   gACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCGAGCGTCTGGATGGTGAGATGAGTGAAGGCGAGCtgg                                >  1:436994/1‑140 (MQ=255)                                                                                                        tCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCCCGCTGAACGGCGAGCGTCTGGGTGGTGAGATGAGGGAAGGCGAGCTGGGGGag                           >  2:605671/1‑139 (MQ=255)                                                                                                        tCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCGAGCGTCTGGGTGGTGAGATGAGTGAAGGCGAGCTGGTGGAt                           >  1:285582/1‑140 (MQ=255)                                                                                                        tCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCGAGCGTCTGGATGGTGAGATGAGTGAAGGCGAGCTGGTGGAt                           >  1:390590/1‑140 (MQ=255)                                                                                                        tCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCGAGCGTCTGGATGGTGAGATGAGGGAAGGCGAGCTGGGGGag                           >  2:552319/1‑139 (MQ=255)                                                                                                        tCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCGAGCGGCTGGGTGGGGAGATGAGTGAAGGCGAGCTGGTGGag                           >  2:561613/1‑139 (MQ=255)                                                                                                        tCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGACCCGCTGAACGGCGGGGGGCTGGGTGGGGGGATGGGTGAAAGGGGGCtggtggcg                           >  2:236798/1‑138 (MQ=255)                                                                                                          cccGAACTGGTTGCCTGGGCCAGAAGCAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCGAGCGTCTGGATGGTGAGATGAGTGAAGGCGAGCTGGTGGATGc                         <  2:1206632/140‑1 (MQ=255)                                                                                                          cccGAACTGGTTGCCTGGACCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCGAGCGTCTGGATGGTGAGATGAGTGAAGGCGAGCTGGTGGATGc                         <  1:682905/140‑1 (MQ=255)                                                                                                              aaCTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCGAGCGTCTGGATGGTGAGATGAGTGAAGGCGAGCTGGTGGATGCATTc                     <  1:160648/140‑1 (MQ=255)                                                                                                               aCTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCGAGCGTCTGGATGGTGAGATGAGTGAAGGCGAGCTGGTGGATGCATTcc                    <  1:1130131/140‑1 (MQ=255)                                                                                                               aCTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCGAGCGTCTGGATGGGGAGATGAGGGAAGGCGAGCTGGTGGATGCATTcc                    >  2:343555/1‑140 (MQ=255)                                                                                                                cTGGTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCGAGCGTCTGGATGGTGAGATGAGTGAAGGCGAGCTGGTGGATGCATTCCg                   <  1:114869/140‑1 (MQ=255)                                                                                                                  ggTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCGAGCGTCTGGATGGTGAGATGAGTGAAGGCGAGCTGGTGGATGCATTCCGcc                 >  1:946827/1‑140 (MQ=255)                                                                                                                   gTTGCCTGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCGAGCGTCTGGATGGTGAGATGAGTGAAGGCGAGCTGGTGGATGCATTCCGCCa                <  2:390590/140‑1 (MQ=255)                                                                                                                         tGGGCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCGAGCGTCTGGATGGTGAGATGAGTGAAGGCGAGCTGGTGGATGCATTCCGCCATgtgag          <  2:526717/140‑1 (MQ=255)                                                                                                                           ggCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCGAGCGTCTGGATGGTGAGATGAGTGAAGGCGAGCTGGTGGATGCATTCCGCCATgtgagtg        >  1:245441/1‑140 (MQ=255)                                                                                                                            gCCAGAAAAAATGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCGAGCGTCTGGATGGTGAGATGAGTGAAGGCGAGCTGGTGGATGCATTCCGCCATgtgagtga       <  2:958180/140‑1 (MQ=255)                                                                                                                                 aaaaaaTGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCGAGCGTCTGGATGGTGAGATGAGTGAAGGCGAGCTGGTGGATGCATTCCGCCATGTGAGTGATGCGt  <  2:1054878/140‑1 (MQ=255)                                                                                                                                 aaaaaaTGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCGAGCGTCTGGATGGTGAGATGAGTGAAGGCGAGCTGGTGGATGCATTCCGCCATGTGAGTGATGCGt  <  1:644677/140‑1 (MQ=255)                                                                                                                                 aaaaaaTGGCTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCGAGCGTCTGGATGGTGAGATGAGTGAAGGCGAGCTGGTGGATGCATTCCGCCATGTGAGTGATGCGt  <  1:600345/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    TTATTGCCGTCAGGAACCGTATATTACCCTGGGGCGTTATATTCATGTCACCGCGCGCAAACCGCAGAGCAAGGATAAAGTATGAGTGAATTTTCCCAGACAGTCCCCGAACTGGTTGCCTGGGCCAGAAAAAATGACTTCTCCATCTCGCTGCCGGTAGACCGACTCTCTTTTCTGCTGGCGGTTGCCACGCTGAACGGCGAGCGTCTGGATGGTGAGATGAGTGAAGGCGAGCTGGTGGATGCATTCCGCCATGTGAGTGATGCGT  >  NC_000913/974238‑974505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑