Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,579,069 +T coding (274/1686 nt) yddA ← ABC transporter family protein YddA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,579,0651.T97.5% 135.2 / ‑3.4 40coding (278/1686 nt)yddAABC transporter family protein YddA
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base T (20/19);  total (20/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCGAATAGTCAGTAGTTTAATTAACCATG‑TTTTGTTTACGGATATTAATACAAAGATCCCCAGCAGGGCAGGGAACCAAAGGACAAGCTGCCAAAGCTTATCGGTTTCTTTCTGGCTTAGCGCATTGAAAAAATCATTATTCCAGTCGTTTAACCAGAC  >  NC_000913/1578932‑1579195
                                                                                                                                      |                                                                                                                                  
gTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCGAATAGTCAGTAGTTTAATTAACCAtg‑tttttg                                                                                                                              >  2:447318/1‑138 (MQ=255)
   aGGGTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCGAATAGTCAGTAGTTTAATTAACCAtg‑tttttgttt                                                                                                                           >  1:25532/1‑135 (MQ=255)
    gggTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCGAATAGTCAGTAGTTTAATTAACCATGTTTTTGTTTa                                                                                                                           <  2:519967/140‑1 (MQ=255)
     ggTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCGAATAGTCAGTAGTTTAATTAACCATGTTTTTGTTTAc                                                                                                                          >  1:228268/1‑140 (MQ=255)
      gTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCGAATAGTCAGTAGTTTAATTAACCATGTTTTTGTTTACg                                                                                                                         <  1:137846/140‑1 (MQ=255)
      gTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCGAATAGTCAGTAGTTTAATTAACCATGTTTTTGTTTACg                                                                                                                         <  2:257528/140‑1 (MQ=255)
        tATCTGTATTTTTATGCTCGCCGTAGATCTGCGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCGAATAGTCAGTAGTTTAATTAACCATGTTTTTGTTTACGGa                                                                                                                       <  1:7565/140‑1 (MQ=255)
          tCTGTATTTTTATGCTCGCCGTAGATCTGCGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCGAATAGTCAGTAGTTTAATTAACCATGTTTTTGTTTACGGata                                                                                                                     >  2:102421/1‑140 (MQ=255)
          tCTGTATTTTTATGCTCGCCGTAGATCTGCGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCGAATAGTCAGTAGTTTAATTAACCATGTTTTTGTTTACGGata                                                                                                                     >  1:161362/1‑140 (MQ=255)
           cTGTATTTTTATGCTCGCCGTAGATCTGCGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCGAATAGTCAGTAGTTTAATTAACCATGTTTTTGTTTACGGatat                                                                                                                    <  1:447318/140‑1 (MQ=255)
           cTGTATTTTTATGCTCGCCGTAGATCTGCGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCGAATAGTCAGTAGTTTAATTAACCATGTTTTTGTTTACGGatat                                                                                                                    <  2:161362/140‑1 (MQ=255)
              tATTTTTATGCTCGCCGTAGATCTGCGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCGAATAGTCAGTAGTTTAATTAACCATGTTTTTGTTTACGGATATTaa                                                                                                                 <  2:328348/140‑1 (MQ=255)
                   ttATGCTCGCCGTAGATCTGCGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCGAATAGTCAGTAGTTTAATTAACCATGTTTTTGTTTACGGATATTAATACaa                                                                                                            <  1:209921/140‑1 (MQ=255)
                   ttATGCTCGCCGTAGATCTGCGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCGAATAGTCAGTAGTTTAATTAACCATGTTTTTGTTTACGGATATTAATACaa                                                                                                            <  2:417410/140‑1 (MQ=255)
                        cTCGCCGTAGATCTGCGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCGAATAGTCAGTAGTTTAATTAACCATGTTTTTGTTTACGGATATTAATACAAAGATc                                                                                                       >  2:288157/1‑140 (MQ=255)
                                    cTGCGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCGAATAGTCAGTAGTTTAATTAACCATGTTTTTGTTTACGGATATTAATACAAAGATCCCCAgcagggca                                                                                           <  2:36163/140‑1 (MQ=255)
                                    cTGCGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCGAATAGTCAGTAGTTTAATTAACCATGTTTTTGTTTACGGATATTAATACAAAGATCCCCAgcagggca                                                                                           <  1:511338/140‑1 (MQ=255)
                                       cGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCGAATAGTCAGTAGTTTAATTAACCATGTTTTTGTTTACGGATATTAATACAAAGATCCCcag                                                                                                  >  1:248237/1‑130 (MQ=255)
                                       cGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCGAATAGTCAGTAGTTTAATTAACCATGTTTTTGTTTACGGATATTAATACAAAGATCCCcag                                                                                                  <  2:248237/130‑1 (MQ=255)
                                       cGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCGAATAGTCAGTAGTTTAATTAACCATGTTTTTGTTTACGGATATTAATACAAAGATCCCCAGcagggcaggg                                                                                        >  1:35692/1‑140 (MQ=255)
                                       cGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCGAATAGTCAGTAGTTTAATTAACCATGTTTTTGTTTACGGATATTAATACAAAGATCCCCAGcagggcaggg                                                                                        >  2:496108/1‑140 (MQ=255)
                                                 tagtTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCGAATAGTCAGTAGTTTAATTAACCATGTTTTTGTTTACGGATATTAATACAAAGATCCCCAGCAGGGCAGGGAACCAAAGGa                                                                              >  1:286902/1‑140 (MQ=255)
                                                                       gATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCGAATAGTCAGTAGTTTAATTAACCATGTTTTTGTTTACGGATATTAATACAAAGATCCCCAGCAGGGCAGGGAACCAAAGGACAAGCTGCCAAAGCTTATCGGt                                                        <  1:102421/140‑1 (MQ=255)
                                                                             ggTAGTAATCTGTTAACCATTCGCGCCAGCGAATAGTCAGTAGTTTAATTAACCATGTTTTTGTTTACGGATATTAATACAAAGATCCCCAGCAGGGCAGGGAACCAAAGGACAAGCTGCCAAAGCTTATCGGtttcttt                                                  >  1:286522/1‑140 (MQ=255)
                                                                             ggTAGTAATCTGTTAACCATTCGCGCCAGCGAATAGTCAGTAGTTTAATTAACCATGTTTTTGTTTACGGATATTAATACAAAGATCCCCAGCAGGGCAGGGAACCAAAGGACAAGCTGCCAAAGCTTATCGGtttcttt                                                  <  2:484987/140‑1 (MQ=255)
                                                                                     tCTGTTAACCATTCGCGCCAGCGAATAGTCAGTAGTTTAATTAACCATG‑TTTTGTTTACGGATATTAATACAAAGATCCCCAGCAGGGCAGGGAACCAAAGGACAAGCTGCCAAAGCTTATCGGTTTCTTTCTGGCTTAg                                         <  2:80542/140‑1 (MQ=255)
                                                                                       tGTTAACCATTCGCGCCAGCGAATAGTCAGTAGTTTAATTAACCATGTTTTTGTTTACGGATATTAATACAAAGATCCCCAGCAGGGCAGGGAACCAAAGGACAAGCTGCCAAAGCTTATCGGTTTCTTTCTGGCTTAgc                                        >  1:436841/1‑140 (MQ=255)
                                                                                       tGTTAACCATTCGCGCCAGCGAATAGTCAGTAGTTTAATTAACCATGTTTTTGTTTACGGATATTAATACAAAGATCCCCAGCAGGGCAGGGAACCAAAGGACAAGCTGCCAAAGCTTATCGGTTTCTTTCTGGCTTAgc                                        >  1:205735/1‑140 (MQ=255)
                                                                                          tAACCATTCGCGCCAGCGAATAGTCAGTAGTTTAATTAACCATGTTTTTGTTTACGGATATTAATACAAAGATCCCCAGCAGGGCAGGGAACCAAAGGACAAGCTGCCAAAGCTTATCGGTTTCTTTCTGGCTTAGCGCa                                     <  2:436841/140‑1 (MQ=255)
                                                                                            aCCATTCGCGCCAGCGAATAGTCAGTAGTTTAATTAACCATGTTTTTGTTTACGGATATTAATACAAAGATCCCCAGCAGGGCAGGGAACCAAAGGACAAGCTGCCAAAGCTTATCGGTTTCTTTCTGGCTTAGCGCAtt                                   >  1:135035/1‑140 (MQ=255)
                                                                                                ttCGCGCCAGCGAATAGTCAGTAGTTTAATTAACCATGTTTTTGTTTACGGATATTAATACAAAGATCCCCAGCAGGGCAGGGAACCAAAGGACAAGCTGCCAAAGCTTATCGGTTTCTTTCTGGCTTAGCGCATTGaaa                               >  1:452290/1‑140 (MQ=255)
                                                                                                ttCGCGCCAGCGAATAGTCAGTAGTTTAATTAACCATGTTTTTGTTTACGGATATTAATACAAAGATCCCCAGCAGGGCAGGGAACCAAAGGACAAGCTGCCAAAGCTTATCGGTTTCTTTCTGGCTTAGCGCATTGaaa                               >  2:240591/1‑140 (MQ=255)
                                                                                                 tCGCGCCAGCGAATAGTCAGTAGTTTAATTAACCATGTTTTTGTTTACGGATATTAATACAAAGATCCCCAGCAGGGCAGGGAACCAAAGGACAAGCTGCCAAAGCTTATCGGTTTCTTTCTGGCTTAGCGCATTGaaaa                              >  2:104120/1‑140 (MQ=255)
                                                                                                 tCGCGCCAGCGAATAGTCAGTAGTTTAATTAACCATGTTTTTGTTTACGGATATTAATACAAAGATCCCCAGCAGGGCAGGGAACCAAAGGACAAGCTGCCAAAGCTTATCGGTTTCTTTCTGGCTTAGCGCATTGaaaa                              >  2:369991/1‑140 (MQ=255)
                                                                                                     gcCAGCGAATAGTCAGTAGTTTAATTAACCATGTTTTTGTTTACGGATATTAATACAAAGATCCCCAGCAGGGCAGGGAACCAAAGGACAAGCTGCCAAAGCTTATCGGTTTCTTTCTGGCTTAGCGCATTGAAAAAATc                          <  2:194468/140‑1 (MQ=255)
                                                                                                              tAGTCAGTAGTTTAATTAACCATGTTTTTGTTTACGGATATTAATACAAAGATCCCCAGCAGGGCAGGGAACCAAAGGACAAGCTGCCAAAGCTTATCGGTTTCTTTCTGGCTTAGCGCATTGAAAAAATCATTATTCCa                 <  1:288157/140‑1 (MQ=255)
                                                                                                                      agtTTAATTAACCATGTTTTTGTTTACGGATATTAATACAAAGATCCCCAGCAGGGCAGGGAACCAAAGGACAAGCTGCCAAAGCTTATCGGTTTCTTTCTGGCTTAGCGCATTGAAAAAATCATTATTCCAGTCGTTTa         >  1:34768/1‑140 (MQ=255)
                                                                                                                        tttAATTAACCATGTTTTTGTTTACGGATATTAATACAAAGATCCCCAGCAGGGCAGGGAACCAAAGGACAAGCTGCCAAAGCTTATCGGTTTCTTTCTGGCTTAGCGCATTGAAAAAATCATTATTCCAGTCGTTTAAc       >  2:229272/1‑140 (MQ=255)
                                                                                                                          taattaaCCATGTTTTTGTTTACGGATATTAATACAAAGATCCCCAGCAGGGCAGGGAACCAAAGGACAAGCTGCCAAAGCTTATCGGTTTCTTTCTGGCTTAGCGCATTGAAAAAATCATTATTCCAGTCGTTTAACCa     <  1:133026/140‑1 (MQ=255)
                                                                                                                          taattaaCCATGTTTTTGTTTACGGATATTAATACAAAGATCCCCAGCAGGGCAGGGAACCAAAGGACAAGCTGCCAAAGCTTATCGGTTTCTTTCTGGCTTAGCGCATTGAAAAAATCATTATTCCAGTCGTTTAACCa     <  2:205735/140‑1 (MQ=255)
                                                                                                                          taattaaCCATGTTTTTGTTTACGGATATTAATACAAAGATCCCCAGCAGGGCAGGGAACCAAAGGACAAGCTGCCAAAGCTTATCGGTTTCTTTCTGGCTTAGCGCATTGAAAAAATCATTATTCCAGTCGTTTAACCa     >  1:353369/1‑140 (MQ=255)
                                                                                                                             ttaaCCATGTTTTTGTTTACGGATATTAATACAAAGATCCCCAGCAGGGCAGGGAACCAAAGGACAAGCTGCCAAAGCTTATCGGTTTCTTTCTGGCTTAGCGCATTGAAAAAATCATTATTCCAGTCGTTTAACCAGAc  >  1:82684/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                  
GTCAGGGTTATCTGTATTTTTATGCTCGCCGTAGATCTGCGTGAAGTAGTAGTTTTTATCTGCAAACCACCGATTAAGGTAGTAATCTGTTAACCATTCGCGCCAGCGAATAGTCAGTAGTTTAATTAACCATG‑TTTTGTTTACGGATATTAATACAAAGATCCCCAGCAGGGCAGGGAACCAAAGGACAAGCTGCCAAAGCTTATCGGTTTCTTTCTGGCTTAGCGCATTGAAAAAATCATTATTCCAGTCGTTTAACCAGAC  >  NC_000913/1578932‑1579195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑