Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,398,849	G→T	V226V (GTC→GTA)	fnr ←	DNA‑binding transcriptional dual regulator FNR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,398,849	0	G	T	100.0%	106.8 / NA	31	V226V (GTC→GTA)	fnr	DNA‑binding transcriptional dual regulator FNR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/17);  total (14/17)

ATCAATAAATCAGAAAAATTTAATGATATGACAGAAGGATAGTGAGTTATGCGGAAAAATCAGGCAACGTTACGCGTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAA  >  NC_000913/1398715‑1398985                                                                                                                                       |                                                                                                                                         aTCAATAAATCAGAAAAATTTAATGATATGACAGAAGGATAGTGAGTTATGCGGAAAAATCAGGCAACGTTACGCGTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTTACTGc                                                                                                                                     >  1:505584/1‑140 (MQ=255)    aaTAAATCAGAAAAATTTAATGATATGACAGAAGGATAGTGAGTTATGCGGAAAAATCAGGCAACGTTACGCGTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTTACTGCCAg                                                                                                                                  >  2:531592/1‑140 (MQ=255)     aTAAATCAGAAAAATTTAATGATATGACAGAAGGATAGTGAGTTATGCGGAAAAATCAGGCAACGTTACGCGTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTTACTGCCAGc                                                                                                                                 >  1:108448/1‑140 (MQ=255)        aaTCAGAAAAATTTAATGATATGACAGAAGGATAGTGAGTTATGCGGAAAAATCAGGCAACGTTACGCGTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTTACTGCCAGCATg                                                                                                                              <  1:516074/140‑1 (MQ=255)           cAGAAAAATTTAATGATATGACAGAAGGATAGTGAGTTATGCGGAAAAATCAGGCAACGTTACGCGTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTTACTGCCAGCATGCCg                                                                                                                           >  1:299341/1‑140 (MQ=255)                            atGACAGAAGGATAGTGAGTTATGCGGAAAAATCAGGCAACGTTACGCGTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTTACTGCCAGCATGCCGCTTTTCTGGAAGCGAcc                                                                                                          <  1:379710/140‑1 (MQ=255)                                     ggATAGTGAGTTATGCGGAAAAATCAGGCAACGTTACGCGTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTTACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACg                                                                                                 <  2:273318/140‑1 (MQ=255)                                           tGAGTTATGCGGAAAAATCAGGCAACGTTACGCGTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTTACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGAt                                                                                           <  2:16465/140‑1 (MQ=255)                                           tGAGTTATGCGGAAAAATCAGGCAACGTTACGCGTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTTACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGAt                                                                                           <  1:271962/140‑1 (MQ=255)                                               ttATGCGGAAAAATCAGGCAACGTTACGCGTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTTACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGtt                                                                                       >  2:448060/1‑140 (MQ=255)                                                tATGCGGAAAAATCAGGCAACGTTACGCGTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTTACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGttt                                                                                      >  2:427770/1‑140 (MQ=255)                                                 aTGCGGAAAAATCAGGCAACGTTACGCGTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTTACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTc                                                                                     >  2:371957/1‑140 (MQ=255)                                                  tGCGGAAAAATCAGGCAACGTTACGCGTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTTACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCt                                                                                    <  1:398657/140‑1 (MQ=255)                                                    cGGAAAAATCAGGCAACGTTACGCGTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTTACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTAc                                                                                  >  2:369162/1‑140 (MQ=255)                                                    cGGAAAAATCAGGCAACGTTACGCGTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTTACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTAc                                                                                  >  1:527632/1‑140 (MQ=255)                                                             cAGGCAACGTTACGCGTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTTACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGcc                                                                         <  1:531592/140‑1 (MQ=255)                                                             cAGGCAACGTTACGCGTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTTACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGcc                                                                         <  2:299183/140‑1 (MQ=255)                                                             cAGGCAACGTTACGCGTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTTACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGcc                                                                         <  1:181534/140‑1 (MQ=255)                                                                            gTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTTACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGat                                                          <  2:527632/140‑1 (MQ=255)                                                                               tGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTTACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATc                                                       >  1:91044/1‑140 (MQ=255)                                                                                    aGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTTACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCAc                                                  <  1:427770/140‑1 (MQ=255)                                                                                      cAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTTACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGa                                                >  1:254069/1‑140 (MQ=255)                                                                                             ggCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTTACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCg                                         >  1:159534/1‑140 (MQ=255)                                                                                               ccAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTTACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTc                                       <  1:400098/140‑1 (MQ=255)                                                                                                cAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTTACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCa                                      >  2:230003/1‑140 (MQ=255)                                                                                                   cgcATCGTTATTTTCGATGGTGATGTATTTACCTTTTACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGc                                   >  2:527515/1‑140 (MQ=255)                                                                                                       tCGTTATTTTCGATGGTGATGTATTTACCTTTTACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGaa                               <  2:257317/140‑1 (MQ=255)                                                                                                       tCGTTATTTTCGATGGTGATGTATTTACCTTTTACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGaa                               <  1:480502/140‑1 (MQ=255)                                                                                                             ttttCGATGGTGATGTATTTACCTTTTACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACg                         <  1:95765/140‑1 (MQ=255)                                                                                                             ttttCGATGGTGATGTATTTACCTTTTACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACg                         <  1:369162/140‑1 (MQ=255)                                                                                                             ttttCGATGGTGATGTATTTACCTTTTACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACg                         <  2:502830/140‑1 (MQ=255)                                                                                                                                    ttttACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCCGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCaa  <  2:227794/140‑1 (MQ=255)                                                                                                                                    ttttACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCaa  <  2:109298/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                         ATCAATAAATCAGAAAAATTTAATGATATGACAGAAGGATAGTGAGTTATGCGGAAAAATCAGGCAACGTTACGCGTATGACCAGCAAGCTGGGCCAGCGCATCGTTATTTTCGATGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAA  >  NC_000913/1398715‑1398985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑