Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,602,087	C→A	L200M (CTG→ATG)	lsrA →	Autoinducer‑2 ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,602,087	0	C	A	100.0%	57.0 / NA	18	L200M (CTG→ATG)	lsrA	Autoinducer‑2 ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (11/7);  total (11/7)

CGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTACCCCTGCGGAAACCGAACGCTTGTTTAGTCGCTTGCAAGAGCTGCTTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAA  >  NC_000913/1601967‑1602216                                                                                                                         |                                                                                                                                  cgGATTCTGATCCTCGATGAACCTACCGCCTCGCTTACCCCTGCGGAAACCGAACGCTTGTTTAGTCGCTTGCAAGAGCTGCTTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGATGCCGGAAATTCGCCAGAt                                                                                                                <  1:235220/140‑1 (MQ=255)                    aaCCTACCGCCTCGCTTACCCCTGCGGAAACCGAACGCTTGTTTAGTCGCTTGCAAGAGCTGCTTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGATGCCGGAAATTCGCCAGATTGCCGATCGAATTAGCGTg                                                                                             >  2:69266/1‑140 (MQ=255)                                cGCTTACCCCTGCGGAAACCGAACGCTTGTTTAGTCGCTTGCAAGAGCTGCTTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGATGCCGGAAATTCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGa                                                                                 >  2:509328/1‑140 (MQ=255)                                           gCGGAAACCGAACGCTTGTTTAGTCGCTTGCAAGAGCTGCTTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGATGCCGGAAATTCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCtt                                                                      >  1:277934/1‑140 (MQ=255)                                             ggAAACCGAACGCTTGTTTAGTCGCTTGCAAGAGCTGCTTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGATGCCGGAAATTCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTaa                                                                    >  1:517981/1‑140 (MQ=255)                                                aaCCGAACGCTTGTTTAGTCGCTTGCAAGAGCTGCTTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGATGCCGGAAATTCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCg                                                                 >  1:193478/1‑140 (MQ=255)                                                 aCCGAACGCTTGTTTAGTCGCTTGCAAGAGCTGCTTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGATGCCGGAAATTCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCgg                                                                >  2:284706/1‑140 (MQ=255)                                                            gTTTAGTCGCTTGCAAGAGCTGCTTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGATGCCGGAAATTCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCg                                                     <  2:193478/140‑1 (MQ=255)                                                              ttAGTCGCTTGCAAGAGCTGCTTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGATGCCGGAAATTCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGaa                                                   <  2:94417/140‑1 (MQ=255)                                                                              gctgctTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGATGCCGGAAATTCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACa                                   <  1:206155/140‑1 (MQ=255)                                                                                    tgctACTGGCGTGGGTATTGTTTTTATCTCGCATAAGATGCCGGAAATTCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTc                             <  2:517981/140‑1 (MQ=255)                                                                                     gctACTGGCGTGGGTATTGTTTTTATCTCGCATAAGATGCCGGAAATTCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCa                            <  1:284706/140‑1 (MQ=255)                                                                                       tACTGGCGTGGGTATTGTTTTTATCTCGCATAAGATGCCGGAAATTCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAgg                          >  1:144039/1‑140 (MQ=255)                                                                                       tACTGGCGTGGGTATTGTTTTTATCTCGCATAAGATGCCGGAAATTCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAgg                          >  2:388224/1‑140 (MQ=255)                                                                                           ggCGTGGGTATTGTTTTTATCTCGCATAAGATGCCGGAAATTCGCCAGATTGCCGATCGAAATAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCAt                      >  2:338575/1‑140 (MQ=255)                                                                                                 ggTATTGTTTTTATCTCGCATAAGATGCCGGAAATTCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCAcccc                >  2:87600/1‑140 (MQ=255)                                                                                                            tATCTCGCATAAGATGCCGGAAATTCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACggg     <  1:509328/140‑1 (MQ=255)                                                                                                               ctcGCATAAGATGCCGGAAATTCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGaaa  >  1:463481/1‑140 (MQ=255)                                                                                                                         |                                                                                                                                  CGGATTCTGATCCTCGATGAACCTACCGCCTCGCTTACCCCTGCGGAAACCGAACGCTTGTTTAGTCGCTTGCAAGAGCTGCTTGCTACTGGCGTGGGTATTGTTTTTATCTCGCATAAGCTGCCGGAAATTCGCCAGATTGCCGATCGAATTAGCGTGATGCGCGACGGAACCATCGCCTTAAGCGGCAAAACCAGCGAACTGTCTACCGACGACATTATTCAGGCCATCACCCCAGCGGTACGGGAAA  >  NC_000913/1601967‑1602216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑