Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,788,325	G→T	intergenic (+47/‑110)	ppsR → / → aroH	posphoenolpyruvate synthetase regulatory protein/3‑deoxy‑7‑phosphoheptulonate synthase, Trp‑sensitive

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,788,325	0	G	T	100.0%	100.6 / NA	29	intergenic (+47/‑110)	ppsR/aroH	posphoenolpyruvate synthetase regulatory protein/3‑deoxy‑7‑phosphoheptulonate synthase, Trp‑sensitive
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (18/11);  total (18/11)

ATCCCGTGGATTAACAGTACCAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAGAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCATCACTTCCCGGCAGTCCTGCCGTAGAAGCAACAAATTTCTGAGACTTGTAATGAACAGAACTGACGAACTCCGTACT  >  NC_000913/1788189‑1788461                                                                                                                                         |                                                                                                                                         aTCCCGTGGATTAACAGTACCAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAGAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCTGCg                                                                                                                                       <  2:505452/140‑1 (MQ=255)                   aCCAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAGAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCTGCGAGGTGTGACACACCTCGc                                                                                                                     >  1:267969/1‑140 (MQ=255)                    ccAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAGAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCTGCGAGGTGTGACACACCTCGCa                                                                                                                    >  2:179913/1‑140 (MQ=255)                       attattCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAGAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCTGCGAGGTGTGACACACCTCGCACtt                                                                                                                 >  1:460506/1‑140 (MQ=255)                            tCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAGAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCTGCGAGGTGTGACACACCTCGCACTTGAAAt                                                                                                            >  1:381575/1‑140 (MQ=255)                            tCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAGAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCTGCGAGGTGTGACACACCTCGCACTTGAAAt                                                                                                            >  1:383901/1‑140 (MQ=255)                                tAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAGAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCTGCGAGGTGTGACACACCTCGCACTTGAAATcagc                                                                                                        <  1:179913/140‑1 (MQ=255)                                     gagaTTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAGAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCTGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCgg                                                                                                   >  1:203409/1‑139 (MQ=255)                                        aTTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAGAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCTGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTg                                                                                                >  1:342197/1‑140 (MQ=255)                                                      ccTCGATATCATGGGCCTTAGTCGCCGAATGTACTAGAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCTGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATgc                                                                                  >  2:249024/1‑140 (MQ=255)                                                       cTCGATATCATGGGCCTTAGTCGCCGAATGTACTAGAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCTGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATgcg                                                                                 >  1:281713/1‑140 (MQ=255)                                                          gATATCATGGGCCTTAGTCGCCGAATGTACTAGAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCTGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCAt                                                                              <  2:381575/140‑1 (MQ=255)                                                          gATATCATGGGCCTTAGTCGCCGAATGTACTAGAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCTGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCAt                                                                              <  2:383901/140‑1 (MQ=255)                                                               cATGGGCCTTAGTCGCCGAATGTACTAGAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCTGCGAGGGGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCATCACtt                                                                         >  1:384812/1‑140 (MQ=255)                                                                        tAGTCGCCGAATGTACTAGAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCTGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCATCACTTCCCGGCAGt                                                                >  1:126518/1‑140 (MQ=255)                                                                         aGTCGCCGAATGTACTAGAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCTGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCATCACTTCCCGGCAGTc                                                               <  1:340576/140‑1 (MQ=255)                                                                                   tGTACTAGAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCTGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCATCACTTCCCGGCAGTCCTGCCGTAGa                                                     >  1:107864/1‑140 (MQ=255)                                                                                               cTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCTGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCATCACTTCCCGGCAGTCCTGCCGTAGAAGCAACAAAttt                                         >  1:99224/1‑140 (MQ=255)                                                                                               cTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCTGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCATCACTTCCCGGCAGTCCTGCCGTAGAAGCAACAAAttt                                         >  2:243222/1‑140 (MQ=255)                                                                                                  gTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCTGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCATCACTTCCCGGCAGTCCTGCCGTAGAAGCAACAAATTTCTg                                      >  1:64102/1‑140 (MQ=255)                                                                                                     cATTAGCTTATTTTTTTGTTATCATGCTAACCACCCTGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCATCACTTCCCGGCAGTCCTGCCGTAGAAGCAACAAATTTCTgaga                                   >  2:497528/1‑140 (MQ=255)                                                                                                      aTTAGCTTATTTTTTTGTTATCATGCTAACCACCCTGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCATCACTTCCCGGCAGTCCTGCCGTAGAAGCAACAAATTTCTGAGAc                                  <  2:267969/140‑1 (MQ=255)                                                                                                                   tttGTTATCATGCTAACCACCCTGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCATCACTTCCCGGCAGTCCTGCCGTAGAAGCAACAAATTTCTGAGACTTGTAATGAACAg                     <  2:64102/140‑1 (MQ=255)                                                                                                                          tCATGCTAACCCCCCTGCGAGGTGTGACACCCCTCGCACTTTAAATCACCAGCGATTGTTTTATCGGGATGCGCATATCTTCCCGGAAGTCCTGCCGTAGAAGCAACAAATTTCTGAGACTTGTAATGAACAGAACTGAc              <  2:384812/140‑1 (MQ=255)                                                                                                                          tCATGCTAACCACCCTGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCATCACTTCCCGGCAGTCCTGCCGTAGAAGCAACAAATTTCTGAGACTTGTAATGAACAGAACTGAc              <  1:497528/140‑1 (MQ=255)                                                                                                                               cTAACCACCCTGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCATCACTTCCCGGCAGTCCTGCCGTAGAAGCAACAAATTTCTGAGACTTGTAATGAACAGAACTGACGAACt         <  1:212094/140‑1 (MQ=255)                                                                                                                                    caccCTGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCATCACTTCCCGGCAGTCCTGCCGTAGAAGCAACAAATTTCTTAGACTTGTAATGAACAGAACTGACGAACTCCGTa    >  1:376507/1‑140 (MQ=255)                                                                                                                                    caccCTGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCATCACTTCCCGGCAGTCCTGCCGTAGAAGCAACAAATTTCTGAGACTTGTAATGAACAGAACTGAc              <  2:26013/130‑1 (MQ=255)                                                                                                                                    caccCTGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCATCACTTCCCGGCAGTCCTGCCGTAGAAGCAACAAATTTCTGAGACTTGTAATGAACAGAACTGAc              >  1:26013/1‑130 (MQ=255)                                                                                                                                      cccTGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCATCACTTCCCGGCAGTCCTGCCGTAGAAGCAACAAATTTCTGAGACTTGTAATGAACAGAACTGACGAACTCCGTACt  <  1:533585/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         ATCCCGTGGATTAACAGTACCAATTATTCGGTAGAAGAGATTGCCACCAAGATCCTCGATATCATGGGCCTTAGTCGCCGAATGTACTAGAGAACTAGTGCATTAGCTTATTTTTTTGTTATCATGCTAACCACCCGGCGAGGTGTGACACACCTCGCACTTGAAATCAGCAGCGATTGGTTTATCGTGATGCGCATCACTTCCCGGCAGTCCTGCCGTAGAAGCAACAAATTTCTGAGACTTGTAATGAACAGAACTGACGAACTCCGTACT  >  NC_000913/1788189‑1788461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑