Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,911,663	A→T	intergenic (+14/+32)	yebQ → / ← htpX	putative transporter YebQ/zinc dependent endoprotease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,911,663	0	A	T	100.0%	64.5 / NA	20	intergenic (+14/+32)	yebQ/htpX	putative transporter YebQ/zinc dependent endoprotease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (10/10);  total (10/10)

AGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGTTTACGTATCACTCAGCCACGATCCAGGGCAT‑AAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACC  >  NC_000913/1911540‑1911787                                                                                                                             |                                                                                                                             cgTTTGGAGATAATGGTACCCACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGACTGTGTCAGTGGTTTACGTAGCACTCAGCCACGATCCAGGGCAT‑AAAAAAAGCGCGTCGTTCAGGACGCGCttttt                                                                                                              >  2:133204/2‑140 (MQ=255)     tGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGTTTACGTATCACTCAGCCACGATCCAGGGCAT‑AAAAAAAGCGCGTCGTTCAGGACGCGCTTTTTAGTa                                                                                                          <  1:311762/140‑1 (MQ=255)             aTGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGTTTACGTATCACTCAGCCACGATCCAGGGCATAAAAAAAAGCGCGTCGTTCAGGACGCGCTTTTTAGTATTTACtt                                                                                                   >  2:398792/1‑140 (MQ=255)                 tACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGTTTACGTATCACTCAGCCACGATCCAGGGCAT‑AAAAAAAGCGCGTCGTTCAGGACGCGCTTTTTAGTATTTACTTCatat                                                                                              <  2:190153/140‑1 (MQ=255)                          ctcGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGTTTACGTATCACTCAGCCACGATCCAGGGCAT‑AAAAAAAGCGCGTCGTTCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAgg                                                                                     >  1:337494/1‑140 (MQ=255)                              cTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGTTTACGTATCACTCAGCCACGATCCAGGGCAT‑AAAAAAAGCGCGTCGTTCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAGGTAtt                                                                                 <  1:295834/140‑1 (MQ=255)                                        cGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGTTTACGTATCACTCAGCCACGATCCAGGGCAT‑AAAAAAAGCGCGTCGTTCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAGGTATTCACCCGTACg                                                                       <  1:426892/140‑1 (MQ=255)                                                     aGTGATTGCTGCCTGTGTCAGTGGTTTACGTATCACTCAGCCACGATCCAGGGCAT‑AAAAAAAGCGCGTCGTTCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAAtt                                                          >  1:400436/1‑140 (MQ=255)                                                                   gtgtCAGTGGTTTACGTATCACTCAGCCACGATCCAGGGCAT‑AAAAAAAGCGCGTCGTTCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAgcgg                                            >  1:242806/1‑140 (MQ=255)                                                                        aGTGGTTTACGTATCACTCAGCCACGATCCAGGGCAT‑AAAAAAAGCGCGTCGTTCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCgggt                                       <  1:94191/140‑1 (MQ=255)                                                                        aGTGGTTTACGTATCACTCAGCCACGATCCAGGGCAT‑AAAAAAAGCGCGTCGTTCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCgggt                                       <  2:400436/140‑1 (MQ=255)                                                                          tGGTTTACGTATCACTCAGCCACGATCCAGGGCAT‑AAAAAAAGCGCGTCGTTCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCgggtgg                                     >  1:463404/1‑140 (MQ=255)                                                                          tGGTTTACGTATCACTCAGCCACGATCCAGGGCAT‑AAAAAAAGCGCGTCGTTCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCgggtgg                                     >  1:83973/1‑140 (MQ=255)                                                                                aCGTATCACTCAGCCACGATCCAGGGCATAAAAAAAAGCGCGTCGTTCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGTGGGTCAt                                <  1:398792/140‑1 (MQ=255)                                                                                      cACTCAGCCACGATCCAGGGCAT‑AAAAAAAGCGCGTCGTTCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATGaacaac                         >  1:463277/1‑140 (MQ=255)                                                                                       aCTCAGCCACGATCCAGGGCAT‑AAAAAAAGCGCGTCGTTCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGTGGGGGATGagcaacc                        >  2:414554/1‑139 (MQ=255)                                                                                                       gggCAT‑AAAAAAAGCGCGTCGTTCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGCGGGTCATGAACAAATCACTCAGCGGTTTCgg        >  2:513374/1‑139 (MQ=255)                                                                                                          cAT‑AAAAAAAGCGCGTCGTTCAGGACGCGCTTTTTAGTATTTACTTCATATTAGTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACtt     <  1:266305/140‑1 (MQ=255)                                                                                                          cAT‑AAAAAAAGCGCGTCGTTCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACtt     <  2:337494/140‑1 (MQ=255)                                                                                                            t‑AAAAAAAGCGCGTCGTTCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTAc   <  2:463277/140‑1 (MQ=255)                                                                                                              aaaaaaaGCGCGTCGTTCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGTGGGGCATGAACAACTCACTGAGCGATTTCGACTTAcc  >  1:325547/1‑140 (MQ=255)                                                                                                                             |                                                                                                                             AGTTTGGAGATAATGGTACACACGTCTCGCTGATGGCTGCGGCTATTCTGGCAGTGATTGCTGCCTGTGTCAGTGGTTTACGTATCACTCAGCCACGATCCAGGGCAT‑AAAAAAAGCGCGTCGATCAGGACGCGCTTTTTAGTATTTACTTCATATTACTTCAGGTATTCACCCGTACGCAGAGCTTCAATTCGTTTATCCAGCGGCGGGTGGGTCATGAACAACTCACTGAGCGATTTCGACTTACC  >  NC_000913/1911540‑1911787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑