Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,337,170	C→A	G751C (GGT→TGT)	gyrA ←	DNA gyrase subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,337,170	0	C	A	100.0%	133.7 / NA	39	G751C (GGT→TGT)	gyrA	DNA gyrase subunit A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (24/15);  total (24/15)

GATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGTGTTGCAGCCCATCGCACGGACAGA  >  NC_000913/2337036‑2337293                                                                                                                                       |                                                                                                                            gATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACAGtttt                                                                                                                        >  2:331497/1‑140 (MQ=255)     tGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACAGTTttgcgt                                                                                                                    <  2:273049/140‑1 (MQ=255)      ggTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACAGTTttgcgtt                                                                                                                   >  2:139910/1‑140 (MQ=255)      ggTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACAGTTttgcg                                                                                                                     >  2:493721/1‑138 (MQ=255)      ggTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACAGTTttgcg                                                                                                                     <  1:493721/138‑1 (MQ=255)        tCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGCAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACAGTTttgcgttgc                                                                                                                 >  1:454755/1‑140 (MQ=255)           cAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGCAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACAGTTTTGCGTTGCGGt                                                                                                              <  2:454755/140‑1 (MQ=255)            aGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACAGTTTTGCGTTGCGGTg                                                                                                             >  1:417620/1‑140 (MQ=255)             gTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACAGTTTTGCGTTGCGGTGa                                                                                                            >  1:380780/1‑140 (MQ=255)                        gTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACAGTTTTGCGTTGCGGTGAGGATTGCGCCa                                                                                                 >  2:83503/1‑140 (MQ=255)                          aCCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACAGTTTTGCGTTGCGGTGAGGATTGCGCCATc                                                                                               >  1:92771/1‑140 (MQ=255)                           ccGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACAGTTTTGCGTTGCGGTGAGGATTGCGCCATCg                                                                                              >  1:161222/1‑140 (MQ=255)                             gcgcCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACAGTTTTGCGTTGCGGTGAGGATTGCGCCATCGcc                                                                                            >  1:128016/1‑140 (MQ=255)                             gcgcCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACAGTTTTGCGTTGCGGTGAGGATTGCGCCATCGcc                                                                                            >  1:789/1‑140 (MQ=255)                             gcgcCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACAGTTTTGCGTTGCGGTGAGGATTGCGCCATCGcc                                                                                            >  1:76530/1‑140 (MQ=255)                              cgcCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACAGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCa                                                                                           >  2:498637/1‑140 (MQ=255)                               gcCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACAGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCAc                                                                                          >  2:240084/1‑140 (MQ=255)                                   acaacTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACAGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAgg                                                                                      <  2:281794/140‑1 (MQ=255)                                   acaacTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACAGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAgg                                                                                      >  1:210493/1‑140 (MQ=255)                                    caacTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACAGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAGGc                                                                                     <  2:789/140‑1 (MQ=255)                                          aaCCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACAGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAGGCACGATc                                                                               <  2:161222/140‑1 (MQ=255)                                          aaCCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACAGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAGGCACGATc                                                                               <  1:240084/140‑1 (MQ=255)                                               ttACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACAGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAGGCACGATCagaga                                                                          >  1:146713/1‑140 (MQ=255)                                                         tAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACAGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTa                                                                <  1:33492/140‑1 (MQ=255)                                                                    gagaTAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACAGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCAcc                                                     <  2:128016/140‑1 (MQ=255)                                                                    gagaTAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACAGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCAcc                                                     <  2:76530/140‑1 (MQ=255)                                                                    gagaTAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACAGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCAcc                                                     <  2:417620/140‑1 (MQ=255)                                                                       aTAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACAGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTaa                                                  >  1:468458/1‑140 (MQ=255)                                                                          aCCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACAGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCg                                               >  1:16292/1‑140 (MQ=255)                                                                            cccTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACAGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGaa                                             <  2:380780/140‑1 (MQ=255)                                                                               tttCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACAGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATAc                                          >  1:484499/1‑140 (MQ=255)                                                                                ttCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACAGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATAcc                                         >  1:307446/1‑140 (MQ=255)                                                                                     cgCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACAGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGaa                                    >  2:532110/1‑140 (MQ=255)                                                                                                      ggTATTCCGCCACTGCGGTACGTTTACCGTAACAGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGTGTTGCAg                   >  2:41992/1‑140 (MQ=255)                                                                                                               ccACTGCGGTACGTTTACCGTAACAGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGTGTTGCAGCCCATCGCa          <  2:146713/140‑1 (MQ=255)                                                                                                                 aCTGCGGTACGTTTACCGTAACAGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGTGTTGCAGCCCATCGCACg        <  2:59937/140‑1 (MQ=255)                                                                                                                  cTGCGGTACGTTTACCGTAACAGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGTGTTGCAGCCCATCGCACgg       >  2:52754/1‑140 (MQ=255)                                                                                                                     cGGTACGTTTACCGTAACAGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGTGTTGCAGCCCATCGCACGGACa    <  1:498637/140‑1 (MQ=255)                                                                                                                       gTACGTTTACCGTAACAGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGTGTTGCAGCCCATCGCACGGACaga  >  1:103699/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                            GATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGTTTTGCGTTGCGGTGAGGATTGCGCCATCGCCACGAGGCACGATCAGAGAGACGACTTTATCGCCTTCACCTAAGCGAATACCGCGAACACCGGTGGTGTTGCAGCCCATCGCACGGACAGA  >  NC_000913/2337036‑2337293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑