Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,468,612	G→T	T133T (ACG→ACT)	yfdH →	CPS‑53 (KpLE1) prophage; bactoprenol glucosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,468,612	0	G	T	100.0%	107.9 / NA	32	T133T (ACG→ACT)	yfdH	CPS‑53 (KpLE1) prophage; bactoprenol glucosyl transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (12/20);  total (12/20)

TCCCAATTGATGTTGACCTGCAAGACCCGATTGAGGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCTGATGAGCCGTGATGTTGTCGAAAATATTAAACTTATGCCAGAACGAAACCTTTTCATGAAAG  >  NC_000913/2468479‑2468748                                                                                                                                      |                                                                                                                                         tCCCAATTGATGTTGACCTGCAAGACCCGATTGAGGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGACGCCTGAAGCTAAAAACTGCTGAg                                                                                                                                    <  2:251509/140‑1 (MQ=255)   ccAATTGATGTTGACCTGCAAGACCCGATTGAGGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACTGCTGAGTg                                                                                                                                  <  1:129418/140‑1 (MQ=255)   ccAATTGATGTTGACCTGCAAGACCCGATTGAGGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACTGCTGAGTg                                                                                                                                  <  2:514623/140‑1 (MQ=255)   ccAATTGATGTTGACCTGCAAGACCCGATTGAGGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACTGCTGAGTg                                                                                                                                  <  1:522452/140‑1 (MQ=255)   ccAATTGATGTTGACCTGCAAGACCCGATTGAGGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGAGGGACGCCTGAAGCGAAAAACTGCTGAGTg                                                                                                                                  <  2:451174/140‑1 (MQ=255)                aCCTGCAAGACCCGATTGAGGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACTGCTGAGTGGTTCTATAAGCTc                                                                                                                     <  1:137605/140‑1 (MQ=255)                aCCTGCAAGACCCGATTGAGGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACTGCTGAGTGGTTCTATAAGCTc                                                                                                                     <  1:142022/140‑1 (MQ=255)                 ccTGCAAGACCCGATTGAGGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACTGCTGAGTGGTTCTATAAGCTcc                                                                                                                    <  2:139390/140‑1 (MQ=255)                    gCAAGACCCGATTGAGGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACTGCTGAGTGGTTCTATAAGCTCcaca                                                                                                                 <  1:428603/140‑1 (MQ=255)                               ttGAGGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACTGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGc                                                                                                      <  1:129784/140‑1 (MQ=255)                               ttGAGGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACTGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGc                                                                                                      <  1:152080/140‑1 (MQ=255)                                        ttCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACTGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTaaa                                                                                             >  1:343097/1‑140 (MQ=255)                                          ccTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACTGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAAt                                                                                           >  2:93416/1‑140 (MQ=255)                                             cATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACTGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGa                                                                                        >  1:17535/1‑140 (MQ=255)                                                   aTTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACTGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGaa                                                                                  >  2:528873/1‑140 (MQ=255)                                                   aTTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACTGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGaa                                                                                  >  2:414433/1‑140 (MQ=255)                                                                 aGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACTGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCa                                                                    <  1:506762/140‑1 (MQ=255)                                                                 aGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACTGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCa                                                                    >  1:506088/1‑140 (MQ=255)                                                                        gCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACTGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCtgat                                                             <  1:7650/140‑1 (MQ=255)                                                                           gATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACTGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCTGATGAg                                                          <  1:528873/140‑1 (MQ=255)                                                                                 gTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACTGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCTGATGAGCCGTGa                                                    >  2:203373/1‑140 (MQ=255)                                                                                          aaaaGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACTGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCTGATGAGCCGTGATGTTGTCGa                                           <  2:311471/140‑1 (MQ=255)                                                                                          aaaaGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACTGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCTGATGAGCCGTGATGTTGTCGa                                           <  2:45457/140‑1 (MQ=255)                                                                                            aaGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACTGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCTGATGAGCCGTGATGTTGTCGaaa                                         <  2:226116/140‑1 (MQ=255)                                                                                                 cTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACTGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCTGATGAGCCGTGATGTTGTCGAAAATAtt                                    <  2:343097/140‑1 (MQ=255)                                                                                                         tCAACTGATGGACGCCTGAAGCGAAAAACTGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCTGATGAGCCGTGATGTTGTCGAAAATATTAAACTTAt                            >  1:451925/1‑140 (MQ=255)                                                                                                                   gACGCCTGAAGCGAAAAACTGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCTGATGAGCCGTGATGTTGTCGAAAATATTAAACTTATGCCAGAACGa                  >  2:80845/1‑140 (MQ=255)                                                                                                                     cGCCTGAAGCGAAAAACTGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCTGATGAGCCGTGATGTTGTCGAAAATATTAAACTTATGCCAGAACGaaa                >  2:529334/1‑140 (MQ=255)                                                                                                                       ccTGAAGCGAAAAACTGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCTGATGAGCCGTGATGTTGTCGAAAATATTAAACTTATGCCAGAACGAAAcc              >  2:523786/1‑140 (MQ=255)                                                                                                                          gAAGCGAAAAACTGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCTGATGAGCCGTGATGTTGTCGAAAATATTAAACTTATGCCAGAACGAAACCttt           <  1:410605/140‑1 (MQ=255)                                                                                                                                aaaaaCTGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCTGATGAGCCGTGATGTTGTCGAAAATATTAAACTTATGCCAGAACGAAACCTTTTCATGa     >  2:427641/1‑140 (MQ=255)                                                                                                                                   aaCTGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCTGATGAGCCGTGATGTTGTCGAAAATATTAAACTTATGCCAGAACGAAACCTTTTCATGAAAg  <  2:506088/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         TCCCAATTGATGTTGACCTGCAAGACCCGATTGAGGTTATTCCTCATCTTATTGAAAAATGGCAAGCAGGTGCTGATATGGTTCTTGCTAAAAGATCTGACCGCTCAACTGATGGACGCCTGAAGCGAAAAACGGCTGAGTGGTTCTATAAGCTCCACAATAAAATAAGCAATCCTAAAATTGAAGAGAATGTTGGTGATTTCAGGCTGATGAGCCGTGATGTTGTCGAAAATATTAAACTTATGCCAGAACGAAACCTTTTCATGAAAG  >  NC_000913/2468479‑2468748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑