Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,513,527	T→G	I38L (ATT→CTT)	trpS ←	tryptophan‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,513,527	0	T	G	100.0%	107.0 / NA	31	I38L (ATT→CTT)	trpS	tryptophan‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (15/16);  total (15/16)

AACAAAAATGGTGCTTTTCTCAGGATCGATACCACAAGCCAGATACAAGGCCAGCGTATCCAGCGTCGCTTTACGCAGCTTCTGTGCATCCTGGCGCACGGTGATCGCGTGTTGGTCAACGATACAGTAAATGCAATGGTAGTCATCCTGCATGTTTACCCACTGACGCAGCGCACCCATGTAGTTACCAATGGTCAATTCACCTGAGGGCTGTGCGCCACTAAAAACGATGGGCTTAGTCATTTTTCGAT  >  NC_000913/3513396‑3513646                                                                                                                                    |                                                                                                                        aaCAAAAATGGTGCTTTTCTCAGGATCGATACCACAAGCCAGATACAAGGCCAGCGTATCCAGCGTCGCTTTACGCAGCTTCTGTGCATCCTGGCGCACGGTGATCGCGTGTTGGTCAACGATACAGTAAAGGCAATGGt                                                                                                                 <  2:422894/140‑1 (MQ=255)              cTTTTCTCAGGATCGATACCACAAGCCAGATACAAGGCCAGCGTATCCAACGTCGCTTTACGCAGCTTCTGTGCATCCTGGCGCACGGTGATCGCGTGTTGGTCAACGATACAGTAAAGGCAATGGTAGTCATCCTGCAt                                                                                                    >  1:255758/1‑140 (MQ=255)                        gATCGATACCACAAGCCAGATACAAGGCCAGCGTATCCAGCGTCGCTTTACGCAGCTTCTGTGCATCCTGGCGCACGGTGATCGCGTGTTGGTCAACGATACAGTAAAGGCAATGGTAGTCATCCTGCATGTTTACCCAc                                                                                          >  2:391906/1‑140 (MQ=255)                             aTACCACAAGCCAGATACAAGGCCAGCGTATCCAGCGTCGCTTTACGCAGCTTCTGTGCATCCTGGCGCACGGTGATCGCGTGTTGGTCAACGATACAGTAAAGGCAATGGTAGTCATCCTGCATGTTTACCCACTGACg                                                                                     >  2:409970/1‑140 (MQ=255)                                  acaAGCCAGATACAAGGCCAGCGTATCCAGCGTCGCTTTACGCAGCTTCTGTGCATCCTGGCGCACGGTGATCGCGTGTTGGTCAACGATACAGTAAAGGCAATGGTAGTCATCCTGCATGTTTACCCACTGACGCAgcg                                                                                <  1:144240/140‑1 (MQ=255)                                  acaAGCCAGATACAAGGCCAGCGTATCCAGCGTCGCTTTACGCAGCTTCTGTGCATCCTGGCGCACGGTGATCGCGTGTTGGTCAACGATACAGTAAAGGCAATGGTAGTCATCCTGCATGTTTACCCACTGACGCAgcg                                                                                <  2:19682/140‑1 (MQ=255)                                  acaAGCCAGATACAAGGCCAGCGTATCCAGCGTCGCTTTACGCAGCTTCTGTGCATCCTGGCGCACGGTGATCGCGTGTTGGTCAACGATACAGTAAAGGCAATGGTAGTCATCCTGCATGTTTACCCACTGACGCAgcg                                                                                <  2:152854/140‑1 (MQ=255)                                      gCCAGATACAAGGCCAGCGTATCCAGCGTCGCTTTACGCAGCTTCTGTGCATCCTGGCGCACGGTGATCGCGTGTTGGTCAACGATACAGTAAAGGCAATGGTAGTCATCCTGCATGTTTACCCACTGACGCAGCGCAcc                                                                            <  2:47497/140‑1 (MQ=255)                                      gCCAGATACAAGGCCAGCGTATCCAGCGTCGCTTTACGCAGCTTCTGTGCATCCTGGCGCACGGTGATCGCGTGTTGGTCAACGATACAGTAAAGGCAATGGTAGTCATCCTGCATGTTTACCCACTGACGCAGCGCAcc                                                                            <  2:255758/140‑1 (MQ=255)                                      gCCAGATACAAGGCCAGCGTATCCAGCGTCGCTTTACGCAGCTTCTGTGCATCCTGGCGCACGGTGATCGCGTGTTGGTCAACGATACAGTAAAGGCAATGGTAGTCATCCTGCATGTTTACCCACTGACGCAGCGCAcc                                                                            <  2:188732/140‑1 (MQ=255)                                       ccAGATACAAGGCCAGCGTATCCAGCGTCGCTTTACGCAGCTTCTGTGCATCCTGGCGCACGGTGATCGCGTGTTGGTCAACGATACAGTAAAGGCAATGGTAGTCATCCTGCATGTTTACCCACTGACGCAGCGCAccc                                                                           <  2:67315/140‑1 (MQ=255)                                          gATACAAGGCCAGCGTATCCAGCGTCGCTTTACGCAGCTTCTGTGCATCCTGGCGCACGGTGATCGCGTGTTGGTCAACGATACAGTAAAGGCAATGGTAGTCATCCTGCATGTTTACCCACTGACGCAGCGCACCCATg                                                                        <  1:409970/140‑1 (MQ=255)                                           aTACAAGGCCAGCGTATCCAGCGTCGCTTTACGCAGCTTCTGTGCATCCTGGCGCACGGTGATCGCGTGTTGGTCAACGATACAGTAAAGGCAATGGTAGTCATCCTGCATGTTTACCCACTGACGCAGCGCACCCATGt                                                                       >  1:204515/1‑140 (MQ=255)                                                 ggCCAGCGTATCCAGCGTCGCTTTACGCAGCTTCTGTGCATCCTGGCGCACGGTGATCGCGTGTTGGTCAACGATACAGTAAAGGCAATGGTAGTCATCCTGCATGTTTACCCACTGACGCAGCGCACCCATGTAGTTAc                                                                 >  2:46801/1‑140 (MQ=255)                                                     aGCGTATCCAGCGTCGCTTTACGCAGCTTCTGTGCATCCTGGCGCACGGTGATCGCGTGTTGGTCAACGATACAGTAAAGGCAATGGTAGTCATCCTGCATGTTTACCCACTGACGCAGCGCACCCATGTAGTTACCAAt                                                             >  2:314235/1‑140 (MQ=255)                                                       cGTATCCAGCGTCGCTTTACGCAGCTTCTGTGCATCCTGGCGCACGGTGATCGCGTGTTGGTCAACGATACAGTAAAGGCAATGGTAGTCATCCTGCATGTTTACCCACTGACGCAGCGCACCCATGTAGTTACCAATgg                                                           >  1:109100/1‑140 (MQ=255)                                                       cGTATCCAGCGTCGCTTTACGCAGCTTCTGTGCATCCTGGCGCACGGTGATCGCGTGTTGGTCAACGATACAGTAAAGGCAATGGTAGTCATCCTGCATG‑TTACCCACTGACGCAGCGCACCCATGTAGTTACCAATGGt                                                          <  2:476072/140‑1 (MQ=255)                                                             cAGCGTCGCTTTACGCAGCTTCTGTGCATCCTGGCGCACGGTGATCGCGTGTTGGTCAACGATACAGTAAAGGCAATGGTAGTCATCCTGCATGTTTACCCACTGACGCAGCGCACCCATGTAGTTACCAATGGTCAAtt                                                     <  1:509249/140‑1 (MQ=255)                                                             cAGCGTCGCTTTACGCAGCTTCTGTGCATCCTGGCGCACGGTGATCGCGTGTTGGTCAACGATACAGTAAAGGCAATGGTAGTCATCCTGCATGTTTACCCACTGACGCAGCGCACCCATGTAGTTACCAATGGTCAAtt                                                     <  2:433243/140‑1 (MQ=255)                                                             cAGCGTCGCTTTACGCAGCTTCTGTGCATCCTGGCGCACGGTGATCGCGTGTTGGTCAACGATACAGTAAAGGCAATGGTAGTCATCCTGCATGTTTACCCACTGACGCAGCGCACCCATGTAGTTACCAATGGTCAAtt                                                     <  1:391906/140‑1 (MQ=255)                                                                cGTCGCTTTACGCAGCTTCTGTGCATCCTGGCGCACGGTGATCGCGTGTTGGTCAACGATACAGTAAAGGCAATGGTAGTCATCCTGCATGTTTACCCACTGACGCAGCGCACCCATGTAGTTACCAATGGTCAATTCAc                                                  >  2:371831/1‑140 (MQ=255)                                                                 gTCGCTTTACGCAGCTTCTGTGCATCCTGGCGCACGGTGATCGCGTGTTGGTCAACGATACAGTAAAGGCAATGGTAGTCATCCTGCATGTTTACCCACTGACGCAGCGCACCCATGTAGTTACCAATGGTCAATTCAcc                                                 >  1:7298/1‑140 (MQ=255)                                                                   cGCTTTACGCAGCTTCTGTGCATCCTGGCGCACGGTGATCGCGTGTTGGTCAACGATACAGTAAAGGCAATGGTAGTCATCCTGCATGTTTACCCACTGACGCAGCGCACCCATGTAGTTACCAATGGTCAATTCACCTg                                               >  1:385790/1‑140 (MQ=255)                                                                                           cTGGCGCACGGTGATCGCGTGTTGGTCAACGATACAGTAAAGGCAATGGTAGTCATCCTGCATGTTTACCCACTGACGCAGCGCACCCATGTAGTTACCAATGGTCAATTCACCTGAGGGCTGTGCGCCACTAAAAACGa                       <  2:204515/140‑1 (MQ=255)                                                                                            tGGCGCACGGTGATCGCGTGTTGGTCAACGATACAGTAAAGGCAATGGTAGTCATCCTGCATGTTTACCCACTGACGCAGCGCACCCATGTAGTTACCAATGGTCAATTCACCTGAGGGCTGTGCGCCACTAAAAACGAt                      <  2:243868/140‑1 (MQ=255)                                                                                             ggCGCACGGTGATCGCGTGTTGGTCAACGATACAGTAAAGGCAATGGTAGTCATCCTGCATGTTTACCCACTGACGCAGCGCACCCATGTAGTTACCAATGGTCAATTCACCTGAGGGCTGTGCGCCACTAAAAACGATg                     <  1:215226/140‑1 (MQ=255)                                                                                               cgcACGGTGATCGCGTGTTGGTCAACGATACAGTAAAGGCAATGGTAGTCATCCTGCATGTTTACCCACTGACGCAGCGCACCCATGTAGTTACCAATGGTCAATTCACCTGAGGGCTGTGCGCCACTAAAAACGATggg                   >  1:246592/1‑140 (MQ=255)                                                                                                 cACGGTGATCGCGTGTTGGTCAACGATACAGTAAAGGCAATGGTAGTCATCCTGCATGTTTACCCACTGACGCAGCGCACCCATGTAGTTACCAATGGTCAATTCACCTGAGGGCTGTGCGCCACTAAAAACGATGGGCt                 >  2:483795/1‑140 (MQ=255)                                                                                                    ggTGATCGCGTGTTGGTCAACGATACAGTAAAGGCAATGGTAGTCATCCTGCATGTTTACCCACTGACGCAGCGCACCCATGTAGTTACCAATGGTCAATTCACCTGAGGGCTGTGCGCCACTAAAAACGATGGGCTTAg              >  2:321310/1‑140 (MQ=255)                                                                                                              tgtTGGTCAACGATACAGTAAAGGCAATGGTAGTCATCCTGCATGTTTACCCACTGACGCAGCGCACCCATGTAGTTACCAATGGTCAATTCACCTGAGGGCTGTGCGCCACTAAAAACGATGGGCTTAGTCATTTTTCg    >  2:366083/1‑140 (MQ=255)                                                                                                                ttGGTCAACGATACAGTAAAGGCAATGGTAGTCATCCTGCATGTTTACCCACTGACGCAGCGCACCCATGTAGTTACCAATGGTCAATTCACCTGAGGGCTGTGCGCCACTAAAAACGATGGGCTTAGTCATTTTTCGAt  >  1:188142/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                        AACAAAAATGGTGCTTTTCTCAGGATCGATACCACAAGCCAGATACAAGGCCAGCGTATCCAGCGTCGCTTTACGCAGCTTCTGTGCATCCTGGCGCACGGTGATCGCGTGTTGGTCAACGATACAGTAAATGCAATGGTAGTCATCCTGCATGTTTACCCACTGACGCAGCGCACCCATGTAGTTACCAATGGTCAATTCACCTGAGGGCTGTGCGCCACTAAAAACGATGGGCTTAGTCATTTTTCGAT  >  NC_000913/3513396‑3513646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑