Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,656,250	C→A	A15S (GCT→TCT)	hdeB ←	periplasmic acid stress chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,656,250	0	C	A	100.0%	149.5 / NA	43	A15S (GCT→TCT)	hdeB	periplasmic acid stress chaperone
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (16/27);  total (16/27)

CAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCAATATGCAAGGAAGTA  >  NC_000913/3656119‑3656386                                                                                                                                    |                                                                                                                                         cagcaTCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGAGCCCATaa                                                                                                                                  >  1:344239/1‑140 (MQ=255)         accaTGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGAGCCCATAAAAATAAAc                                                                                                                          <  2:244885/140‑1 (MQ=255)         accaTGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGAGCCCATAAAAATAAAc                                                                                                                          <  1:221692/140‑1 (MQ=255)           caTGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGAGCCCATAAAAATAAACGc                                                                                                                        >  1:264454/1‑140 (MQ=255)              gCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCGCCAGTGACAAAGCCGCTACAGAGCCCATAAAAATAAACGCttt                                                                                                                     >  1:47033/1‑140 (MQ=255)              gCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGAGCCCATAAAAATaa                                                                                                                            >  2:34761/1‑133 (MQ=255)              gCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGAGCCCATAAAAATaa                                                                                                                            <  1:34761/133‑1 (MQ=255)                 aCCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGAGCCCATAAAAATAAACGCTTTACg                                                                                                                  >  2:261705/1‑140 (MQ=255)                   cGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGAGCCCATAAAAATAAACGCTTTACGga                                                                                                                <  2:132369/140‑1 (MQ=255)                         cATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGAGCCCATAAAAATAAACGCTTTACGGAGAGATg                                                                                                          <  1:396672/140‑1 (MQ=255)                                 ttGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGAGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTc                                                                                                  >  2:30819/1‑140 (MQ=255)                                  tGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCGCCAGTGACAAAGCCGCTACAGAGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCa                                                                                                 <  2:47033/140‑1 (MQ=255)                                  tGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGAGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCa                                                                                                 <  1:4883/140‑1 (MQ=255)                                         aGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGAGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTa                                                                                          <  2:344239/140‑1 (MQ=255)                                          gATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGAGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTaa                                                                                         <  2:428318/140‑1 (MQ=255)                                           aTCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGAGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAAc                                                                                        >  2:290364/1‑140 (MQ=255)                                           aTCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGAGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAAc                                                                                        >  2:348913/1‑140 (MQ=255)                                           aTCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGAGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAAc                                                                                        <  2:212295/140‑1 (MQ=255)                                             cAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGAGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAAccc                                                                                      <  1:369493/140‑1 (MQ=255)                                               aTAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGAGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCAt                                                                                    <  2:452138/140‑1 (MQ=255)                                                tAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGAGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCAtt                                                                                   <  1:30819/140‑1 (MQ=255)                                                    ttCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTACCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGAGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAat                                                                               >  1:348835/1‑140 (MQ=255)                                                     tCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGAGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAAc                                                                                        >  2:433705/1‑130 (MQ=255)                                                     tCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGAGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAAc                                                                                        <  1:433705/130‑1 (MQ=255)                                                     tCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGAGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAata                                                                              <  2:264454/140‑1 (MQ=255)                                                       cTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGAGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAAtata                                                                            <  2:148983/140‑1 (MQ=255)                                                       cTGGCAGGTCATATCTTTAGCGGATTCATTGGCTACCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGAGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAAtata                                                                            <  2:348835/140‑1 (MQ=255)                                                        tGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGAGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATag                                                                           <  2:301667/140‑1 (MQ=255)                                                         ggCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGAGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATaga                                                                          <  1:137983/140‑1 (MQ=255)                                                            aGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGAGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGAt                                                                       <  2:242393/140‑1 (MQ=255)                                                              gTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGAGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAAccc                                                                                      <  1:132728/123‑1 (MQ=255)                                                              gTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGAGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAAccc                                                                                      >  2:132728/1‑123 (MQ=255)                                                                         aGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGAGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACag                                                          >  2:239931/1‑140 (MQ=255)                                                                                            aaCGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGAGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTaa                                       <  1:348913/140‑1 (MQ=255)                                                                                                          gTTCACCAGTGACAAAGCCGCTACAGAGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCgg                         >  1:350082/1‑140 (MQ=255)                                                                                                           ttCACCAGTGACAAAGCCGCTACAGAGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGc                        <  2:205223/140‑1 (MQ=255)                                                                                                           ttCACCAGTGACAAAGCCGCTACAGAGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGc                        <  1:125080/140‑1 (MQ=255)                                                                                                            tCACCAGTGACAAAGCCGCTACAGAGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGcc                       <  1:261705/140‑1 (MQ=255)                                                                                                                  gTGACAAAGCCGCTACAGAGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCa                 <  1:36982/140‑1 (MQ=255)                                                                                                                   tGACAAAGCCGCTACAGAGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCaa                >  2:339550/1‑140 (MQ=255)                                                                                                                   tGACAAAGCCGCTACAGAGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCaa                >  1:8282/1‑140 (MQ=255)                                                                                                                        aaGCCGCTACAGAGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCAATATGc           >  1:261844/1‑140 (MQ=255)                                                                                                                                 cAGAGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCAATATGCAAGGAAGTa  <  2:5477/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                         CAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCGCTACAGCGCCCATAAAAATAAACGCTTTACGGAGAGATGAAATATTCATTTTGTAACCCATTCAATATAGAGATTATATATGAACAGGAGGAATAGCTGCTTTTAAAGAAGATATTCCGGCCTGTTCAATATGCAAGGAAGTA  >  NC_000913/3656119‑3656386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑