Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,120,807	T→C	K314R (AAA→AGA)	hslU ←	ATPase component of the HslVU protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,120,807	0	T	C	100.0%	39.8 / NA	13	K314R (AAA→AGA)	hslU	ATPase component of the HslVU protease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/7);  total (6/7)

TACTGCACGGTGATAGAGGCATTCGGCTCGGTCAGAATACGCTCGAAGTCGCTGGTGGTCAGCGCCTGCAGTTCAACGCGGATTGGCAGACGACCTTGCAGTTCCGGGATCAGGTCAGACGGTTTCGCAATCTGGAACGCGCCAGAAGCGATAAACAGAATGTGGTCAGTTTTGACCATCCCGTGTTTGGTGGAAACGGTGCAACCTTCTACCAGCGGCAGCAGGTCACGCTGAACGCCTTCACGAGAA  >  NC_000913/4120684‑4120932                                                                                                                            |                                                                                                                              tACTGCACGGTGATAGAGGCATTCGGCTCGGTCAGAATACGCTCGAAGTCGCTGGTGGTCAGCGCCTGCAGTTCAACGCGGATTGGCACACGACCTTGCAGTTCCGGGATCAGGTCAGACGGTCTCGCAATCTGGAAcgc                                                                                                               >  2:247973/1‑140 (MQ=255)      cACGGTGATAGAGGCATTCGGCTCGGTCAGAATACGCTCGAAGTCGCTGGTGGTCAGCGCCTGCAGTTCAACGCGGATTGGCAGACGACCTTGCAGTTCCGGGATCAGGTCAGACGGTCTCGCAATCTGGAACGCGCCAg                                                                                                          >  2:107058/1‑140 (MQ=255)                           cTCGGTCAGAATACGCTCGAAGTCGCTGGTGGTCAGCGCCTGCAGTTCAACGCGGATTGGCAGACGACCTTGCAGTTCCGGGATCAGGTCAGACGGTCTCGCAATCTGGAACGCGCCAGAAGCGATAAACAGAATGTGGt                                                                                     <  1:344003/140‑1 (MQ=255)                           cTCGGTCAGAATACGCTCGAAGTCGCTGGTGGTCAGCGCCTGCAGTTCAACGCGGATTGGCAGACGACCTTGCAGTTCCGGGATCAGGTCAGACGGTCTCGCAATCTGGAACGCGCCAGAAGCGATAAACAGAATGTGGt                                                                                     <  1:370503/140‑1 (MQ=255)                           cTCGGTCAGAATACGCTCGAAGTCGCTGGTGGTCAGCGCCTGCAGTTCAACGCGGATTGGCAGACGACCTTGCAGTTCCGGGATCAGGTCAGACGGTCTCGCAATCTGGAACGCGCCAGAAGCGATAAACAGAATGTGGt                                                                                     <  2:351994/140‑1 (MQ=255)                              ggTCAGAATACGCTCGAAGTCGCTGGTGGTCAGCGCCTGCAGTTCAACGCGGATTGGCAGACGACCTTGCAGTTCCGGGATCAGGTCAGACGGTCTCGCAATCTGGAACGCGCCAGAAGCGATAAACAGAATGTGGTCAg                                                                                  >  2:521380/1‑140 (MQ=255)                               gTCAGAATACGCTCGAAGTCGCTGGTGGTCAGCGCCTGCAGTTCAACGCGGATTGGCAGACGACCTTGCAGTTCCGGGATCAGGTCAGACGGTCTCGCAATCTGGAACGCGCCAGAAGCGATAAACAGAATGTGGTCAGt                                                                                 >  1:85401/1‑140 (MQ=255)                                 cAGAATACGCTCGAAGTCGCTGGTGGTCAGCGCCTGCAGTTCAACGCGGATTGGCAGACGACCTTGCAGTTCCGGGATCAGGTCAGACGGTCTCGCAATCTGGAACGCGCCAGAAGCGATAAACAGAATGTGGTCAGttt                                                                               <  1:107058/140‑1 (MQ=255)                                          cTCGAAGTCGCTGGTGGTCAGCGCCTGCAGTTCAACGCGGATTGGAAGACGACCTTGCAGTTCCGGGATCAGGTCAGACGGTCTCGCAATCTGGAACGCGCCAGAAGCGATAAACAGAATGTGGTCAGTTTTGACCATcc                                                                      >  2:479871/1‑140 (MQ=255)                                                       gtggtCAGCGCCTGCAGTTCAACTCGGATTGGCAGACGACCTTGCAGTTCCGGGATCAGGTCAGACGGTCTCGCAATCTATAACGCGCCAGAAGCGATAAACAGAATGTGGTCAGTTTTGACCATCCCGTGTTTGGTGGa                                                         <  2:374024/140‑1 (MQ=255)                                                                                   ttGGCAGACGACCTTGCAGTTCCGGGATCAGGTCAGACGGTCTCGCAATCTGGAACGCGCCAGAAGCGATAAACAGAATGTGGTCAGTTTTGACCATCCCGTGTTTGGTGGAAACGGTGCAACCTTCTACCAGCGgcagc                             <  1:287504/140‑1 (MQ=255)                                                                                                        ccGGGATCAGGTCAGACGGTCTCGCAATCTGGAACGCGCCAGAAGCGATAAACAGAATGTGGTCAGTTTTGACCATCCCGTGTTTGGTGGAAACGGTGCAACCTTCTACCAGCGGCAGCAGGTCACGCTGAACGCCTTCa        >  1:102543/1‑140 (MQ=255)                                                                                                        ccGGGATCAGGTCAGACGGTCTCGCAATCTGGAACGCGCCAGAAGCGATAAACAGAATGTGGTCAGTTTTGACCATCCCGTGTTTGGTGGAAACGGTGCAACCTTCTACCAGCGGCAGCAGGTCACGCTGAACGCCTTCa        >  1:246110/1‑140 (MQ=255)                                                                                                              tCAGGTCAGACGGTCTCGCAATCTGGAACGCGCCAGAAGCGATAAACAGAATGTGGTCAGTTTTGACCATCCCGTGTTTGGTGGAAACGGTGCAACCTTCTACCAGCGGCAGCAGGTCACGCTGAACGCCTTCACGAGaa  <  1:521380/140‑1 (MQ=255)                                                                                                                            |                                                                                                                              TACTGCACGGTGATAGAGGCATTCGGCTCGGTCAGAATACGCTCGAAGTCGCTGGTGGTCAGCGCCTGCAGTTCAACGCGGATTGGCAGACGACCTTGCAGTTCCGGGATCAGGTCAGACGGTTTCGCAATCTGGAACGCGCCAGAAGCGATAAACAGAATGTGGTCAGTTTTGACCATCCCGTGTTTGGTGGAAACGGTGCAACCTTCTACCAGCGGCAGCAGGTCACGCTGAACGCCTTCACGAGAA  >  NC_000913/4120684‑4120932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑