Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,495,163	G→A	A181T (GCC→ACC)	idnK →	D‑gluconate kinase, thermosensitive

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,495,163	0	G	A	100.0%	157.5 / NA	46	A181T (GCC→ACC)	idnK	D‑gluconate kinase, thermosensitive
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/17);  total (29/17)

ACTGGAGCGTCCACAAGCAGATGAACAGGATATTGTGCGCATTGATATCAACCATGATATTGCCAATGTCACCGAACAATGTCGGCAGGCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCGCCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCGCGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCG  >  NC_000913/4495027‑4495290                                                                                                                                         |                                                                                                                                aCTGGAGCGTCCACAAGCAGATGAACAGGATATTGTGCGCATTGATATCAACCATGATATTGCCAATGTCACCGAACAATGTCGGCAGGCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCACCt                                                                                                                              <  1:63154/140‑1 (MQ=255) aCTGGAGCGTCCACAAGCAGATGAACAGGATATTGTGCGCATTGATATCAACCATGATATTGCCAATGTCACCGAACAATGTCGGCAGGCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCACCt                                                                                                                              <  2:261291/140‑1 (MQ=255)   tGGAGCGTCCACAAGCAGATGAACAGGATATTGTGCGCATTGATATCAACCATGATATTGCCAATGTCACCGAACAATGTCGGCAGGCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCACCTCa                                                                                                                            >  2:170043/1‑140 (MQ=255)      aGCGTCCACAAGCAGATGAACAGGATATTGTGCGCATTGATATCAACCATGATATTGCCAATGTCACCGAACAATGTCGGCAGGCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCACCTCAGAt                                                                                                                         <  2:124493/140‑1 (MQ=255)           ccACAAGCAGATGAACAGGATATTGTGCGCATTGATATCAACCATGATATTGCCAATGTCACCGAACAATGTCGGCAGGCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCACCTCAGATCAgcg                                                                                                                    <  1:170043/140‑1 (MQ=255)                    gATGAACAGGATATTGTGCGCATTGATATCAACCATGATATTGCCAATGTCACCGAACAATGTCGGCAGGCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCACCTCAGATCAGCGCTGCGAATg                                                                                                           >  1:177441/1‑140 (MQ=255)                     aTGAACAGGATATTGTGCGCATTGATATCAACCATGATATTGCCAATGTCACCGAACAATGTCGGCAGGCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCACCTCAGATCAGCGCTGCGAATGa                                                                                                          >  1:309251/1‑140 (MQ=255)                               tatTGTGCGCATTGATATCAACCATGATATTGCCAATGTCACCGAACAATGTCGGCAGGCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCACCTCAGATCAGCGCTGCGAATGATTTTCaaaaa                                                                                                >  1:217310/1‑140 (MQ=255)                                atTGTGCGCATTGATATCAACCATGATATTGCCAATGTCACCGAACAATGTCGGCAGGCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCACCTCAGATCAGCGCTGCGAATGATTTTCAAAAAt                                                                                               >  2:505519/1‑140 (MQ=255)                                       gcATTGATATCAACCATGATATTGCCAATGTCACCGAACAATGTCGGCAGGCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCACCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCttt                                                                                        >  2:382794/1‑140 (MQ=255)                                       gcATTGATATCAACCATGATATTGCCAATGTCACCGAACAATGTCGGCAGGCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCACCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCttt                                                                                        >  2:174232/1‑140 (MQ=255)                                       gcATTGATATCAACCATGATATTGCCAATGTCACCGAACAATGTCGGCAGGCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCACCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCttt                                                                                        >  2:227665/1‑140 (MQ=255)                                       gcATTGATATCAACCATGATATTGCCAATGTCACCGAACAATGTCGGCAGGCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCACCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCttt                                                                                        >  1:478646/1‑140 (MQ=255)                                         aTTGATATCAACCATGATATTGCCAATGTCACCGAACAATGTCGGCAGGCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCACCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCa                                                                                      >  1:238404/1‑140 (MQ=255)                                           tGATATCAACCATGATATTGCCAATGTCACCGAACAATGTCGGCAGGCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCACCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAac                                                                                    >  2:97983/1‑140 (MQ=255)                                                   aCCATGATATTGCCAATGTCACCGAACAATGTCGGCAGGCTGTGCTGGCGTTACGACAAAACAGAATATGTGCGAAAGAGGGCAGCACCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCAcgcg                                                                            >  2:58006/1‑140 (MQ=255)                                                       tGATATTGCCAATGTCACCGAACAATGTCGGCAGGCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCACCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTaa                                                                        >  2:3214/1‑140 (MQ=255)                                                       tGATATTGCCAATGTCACCGAACAATGTCGGCAGGCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCACCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTaa                                                                        >  1:229362/1‑140 (MQ=255)                                                       tGATATTGCCAATGTCACCGAACAATGTCGGCAGGCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCACCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTaa                                                                        >  2:502551/1‑140 (MQ=255)                                                              gCCAATGTCACCGAACAATGTCGGCAGGCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCACCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCt                                                                 >  2:332311/1‑140 (MQ=255)                                                               ccAATGTCACCGAACAATGTCGGCAGGCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCACCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAAcg                                                                      <  2:158589/134‑1 (MQ=255)                                                               ccAATGTCACCGAACAATGTCGGCAGGCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCACCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAAcg                                                                      >  1:158589/1‑134 (MQ=255)                                                               ccAATGTCACCGAACAATGTCGGCAGGCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCACCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCtt                                                                >  1:15570/1‑140 (MQ=255)                                                                  aTGTCACCGAACAATGTCGGCAGGCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCACCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTAcc                                                             >  1:177283/1‑140 (MQ=255)                                                                   tGTCACCGAACAATGTCGGCAGGCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCACCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCg                                                            >  2:103994/1‑140 (MQ=255)                                                                        ccGAACAATGTCGGCAGGCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCACCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTcgcg                                                       <  2:15570/140‑1 (MQ=255)                                                                        ccGAACAATGTCGGCAGGCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCACCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTcgcg                                                       >  1:86715/1‑140 (MQ=255)                                                                         cGAACAATGTCGGCAGGCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCACCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCgcgc                                                      <  1:506678/140‑1 (MQ=255)                                                                              aaTGTCGGCAGGCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCACCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCGCGCACATg                                                 <  2:86715/140‑1 (MQ=255)                                                                                tGTCGGCAGGCTGTGCTGGCGATACGACAAAACAGAATATATGCGGAAGAGGGCAGCACCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCGCGCACATGCt                                               >  2:179013/1‑140 (MQ=255)                                                                                     gCAGGCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCACCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCGCGCACATGCTGGATg                                          >  2:43157/1‑140 (MQ=255)                                                                                      cAGGCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCACCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCGCGCACATGCTGGATgg                                         >  2:315429/1‑140 (MQ=255)                                                                                       aGGCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCACCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCGCGCACATGCTGGATGGc                                        >  2:285800/1‑140 (MQ=255)                                                                                        ggCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCACCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCGCGCACATGCTGGATGGcg                                       >  2:438938/1‑140 (MQ=255)                                                                                              gCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCACCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCGCGCACATGCTGGATGGCGTCGTTa                                 <  2:177441/140‑1 (MQ=255)                                                                                                     aTACGACAAAACAGAATATGTGCGAAAGAGGGCAGCACCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCGCGCACATGCTGGATGGCGTCGTTAATTTTCg                          <  1:227665/140‑1 (MQ=255)                                                                                                               aCAGAATATGTGCGAAAGAGGGCAGCACCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCGCGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGaa                <  1:285800/140‑1 (MQ=255)                                                                                                               aCAGAATATGTGCGAAAGAGGGCAGCACCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCGCGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGaa                <  2:478646/140‑1 (MQ=255)                                                                                                               aCAGAATATGTGCGAAAGAGGGCAGCACCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCGCGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGaa                <  1:58006/140‑1 (MQ=255)                                                                                                               aCAGAATATGTGCGAAAGAGGGCAGCACCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCGCGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGaa                <  1:174232/140‑1 (MQ=255)                                                                                                               aCAGAATATGTGCGAAAGAGGGCAGCACCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCGCGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGaa                <  2:177283/140‑1 (MQ=255)                                                                                                                   aaTATGTGCGAAAGAGGGCAGCACCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCGCGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGt            >  1:330344/1‑140 (MQ=255)                                                                                                                          gCGAAAGAGGGCAGCACCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCGCGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCggtgg     <  1:3214/140‑1 (MQ=255)                                                                                                                          gCGAAAGAGGGCAGCACCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCGCGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCggtgg     <  2:309251/140‑1 (MQ=255)                                                                                                                             aaaGAGGGCAGCACCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCGCGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCg  >  2:348439/1‑140 (MQ=255)                                                                                                                             aaaGAGGGCAGCACCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCGCGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCg  >  1:35007/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                ACTGGAGCGTCCACAAGCAGATGAACAGGATATTGTGCGCATTGATATCAACCATGATATTGCCAATGTCACCGAACAATGTCGGCAGGCTGTGCTGGCGATACGACAAAACAGAATATGTGCGAAAGAGGGCAGCGCCTCAGATCAGCGCTGCGAATGATTTTCAAAAATCGGCTTTCAACACCACGCGGTAACGCGCCTTACCGTCGCGCACATGCTGGATGGCGTCGTTAATTTTCGACATCGGGAACAGTTCGGTGGTCG  >  NC_000913/4495027‑4495290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑