Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	554,283	C→G	G52A (GGC→GCC)	ppiB ←	peptidyl‑prolyl cis‑trans isomerase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	554,283	0	C	G	100.0%	101.2 / NA	30	G52A (GGC→GCC)	ppiB	peptidyl‑prolyl cis‑trans isomerase B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (14/16);  total (14/16)

CTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATT  >  NC_000913/554147‑554419                                                                                                                                         |                                                                                                                                         cTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGGCCt                                                                                                                                       >  2:92062/1‑140 (MQ=255)  tgtgCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGGCCTg                                                                                                                                      <  2:226059/140‑1 (MQ=255)    tgCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGGCCTGaa                                                                                                                                    >  1:443570/1‑140 (MQ=255)    tgCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGGCCTGaa                                                                                                                                    <  1:266191/140‑1 (MQ=255)         ttGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGGCCTGAATCATa                                                                                                                               <  2:331974/140‑1 (MQ=255)            cAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGGCCTGAATCATAAAg                                                                                                                            >  1:454601/1‑140 (MQ=255)            cAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGGCCTGAATCATAAAg                                                                                                                            <  2:69135/140‑1 (MQ=255)             agagTGCGGAGCCTGAGTACGTGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGGCCTGAATCATAAAGc                                                                                                                           <  1:196091/140‑1 (MQ=255)                 tGCGGAGCCTAAGTACGTGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGGCCTGAATCATAAAGCCGtt                                                                                                                       >  1:346628/1‑140 (MQ=255)                   cGGAGCCTGAGTACGTGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGGCCTGAATCATAAAGCCGTTGa                                                                                                                     >  1:203913/1‑140 (MQ=255)                   cGGAGCCTGAGTACGTGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGGCCTGAATCATAAAGCCGTTGa                                                                                                                     >  2:177594/1‑140 (MQ=255)                        ccTGAGTACGTGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGGCCTGAATCATAAAGCCGTTGATAaca                                                                                                                <  1:177594/140‑1 (MQ=255)                          tGAGTACGTGCCATTGCCAGCGTACCACGGGTTTTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGGCCTGAATCATAAAGCCGTTGATAACACg                                                                                                              <  1:454707/140‑1 (MQ=255)                           gAGTACGTGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGGCCTGAATCATAAAGCCGTTGATAACACgg                                                                                                             >  2:427663/1‑140 (MQ=255)                           gAGTACGTGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGGCCTGAATCATAAAGCCGTTGATAACACgg                                                                                                             >  1:184791/1‑140 (MQ=255)                                cGTGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGGCCTGAATCATAAAGCCGTTGATAACACGGTGGaa                                                                                                        <  2:346628/140‑1 (MQ=255)                                  tGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGGCCTGAATCATAAAGCCGTTGATAACACGGTGGaaaa                                                                                                      >  1:433668/1‑140 (MQ=255)                                        tGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGGCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGgtgt                                                                                                >  1:61274/1‑140 (MQ=255)                                          ccAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTCTTTTGGTGGCTTTTTTTTCCGTCCCCGTTGCAAACCCGCCGGCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGtgttg                                                                                              <  1:530436/140‑1 (MQ=255)                                            aGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCACCGGCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGtgctgtt                                                                                            >  2:234234/1‑140 (MQ=255)                                             gCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGGCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGtgttgttg                                                                                           >  2:324438/1‑140 (MQ=255)                                                          ttttttCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGGCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTcgc                                                                              <  2:18941/139‑1 (MQ=255)                                                              ttCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGGCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCa                                                                          <  1:162915/140‑1 (MQ=255)                                                                cAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGGCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCagt                                                                        <  1:192694/140‑1 (MQ=255)                                                                cAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGGCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCagt                                                                        <  1:324438/140‑1 (MQ=255)                                                                                 cGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGGCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCAGTAGTCCAGGAAGTTTTTa                                                       <  2:20463/140‑1 (MQ=255)                                                                                                        gCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGGCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTc                                >  2:88543/1‑140 (MQ=255)                                                                                                        gCTTTTTGTTTCAGGCCCGGTTCAAAACCGCCGGCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTc                                <  2:443570/140‑1 (MQ=255)                                                                                                               gTTTCATGCCCGGTTCAAAACCGCCGGCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGtt                         <  1:146831/140‑1 (MQ=255)                                                                                                                   cATGCCCGGTTCAAAACCGCCGGCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTtga                     >  2:398738/1‑140 (MQ=255)                                                                                                                                    cgccggCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGa    <  1:350851/140‑1 (MQ=255)                                                                                                                                    cgccggCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGa    >  1:132724/1‑140 (MQ=255)                                                                                                                                      ccggcCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGAtt  >  1:529094/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         CTGTGCAGTTGCAGAGTGCGGAGCCTGAGTACGTGCCATTGCCAGCGTACCACGGGTATTTTTCAGGCCGTTGTTGGCTTCGTTTTTGATCGGTTCTTTGGTGGCTTTTTGTTTCATGCCCGGTTCAAAACCGCCGCCCTGAATCATAAAGCCGTTGATAACACGGTGGAAAATGGTGTTGTTGTAAAAACCTTCGCGGCAGTAGTCCAGGAAGTTTTTAACTGTTTCAGGTGCTTTATCGTCAAAAGTTTTGATGACAATATCGCCGTGATT  >  NC_000913/554147‑554419

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑