Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,095,929	C→T	G29G (GGC→GGT)	ycdU →	uncharacterized protein YcdU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,095,929	0	C	T	100.0%	155.3 / NA	45	G29G (GGC→GGT)	ycdU	uncharacterized protein YcdU
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/25);  total (20/25)

AGTTATGCATTGTCGTAAAGCATTTAGTGATGCAAAGGTATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGGATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGCTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGGTGTAGTTAGTGGGATTCTGGCTCTGGTGCTGTATAATTTTATTTTTGATCTCAAGGCGTACTGGGCTTATAAATGCGTCACGAAGAATA  >  NC_000913/1095798‑1096065                                                                                                                                    |                                                                                                                                         aGTTATGCATTGTCGTAAAGCATTTAGTGATGCAAAGGTATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGGATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGTTTTTATTg                                                                                                                                  <  1:139705/140‑1 (MQ=255) aGTTATACATTGTCGTAAAGCATTTAGTGATGCAAAGGTATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGGATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGTTTTTATTg                                                                                                                                  >  1:515346/1‑140 (MQ=255)  gTTATGCATTGTCGTAAAGCATTTAGTGATGCAAAGGTATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGGATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGTTTTTATTgg                                                                                                                                 <  1:200866/140‑1 (MQ=255)         aTTGTCGTAAAGCATTTAGTGATGCAAAGGTATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGGATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGTTTTTATTGGAAGAATg                                                                                                                          <  1:477079/140‑1 (MQ=255)           tGTCGTAAAGCATTTAGTGATGCAAAGGTATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGGATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGTTTTTATTGGAAGAATGCa                                                                                                                        <  2:80688/140‑1 (MQ=255)             tCGTAAAGCATTTAGTGATGCAAAGGTATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGGATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGTTTTTATTGGAAGAATGCAgg                                                                                                                      >  2:89618/1‑140 (MQ=255)                       tttAGTGATGCAAAGGTATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGGATAAACGAAATCTGATATACATTTATGCTATTGGACTTAATCTGATAGGTTTTTATTGGAAGAATGCAGGGTTTACtttt                                                                                                            >  2:187251/1‑140 (MQ=255)                           gTGATGCAAAGGTATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGGATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGTTTTTATTGGAAGAATGCAGGGTTTACTTTTCCAt                                                                                                        <  1:426296/140‑1 (MQ=255)                               tGCAAAGGTATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGGATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGTTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCaga                                                                                                    >  2:110624/1‑140 (MQ=255)                                  aaaGGTATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGGATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGTTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGa                                                                                                 <  2:226419/140‑1 (MQ=255)                                  aaaGGTATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGGATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGTTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGa                                                                                                 <  1:491095/140‑1 (MQ=255)                                    aGGTATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGGATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGTTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGata                                                                                               <  1:202661/140‑1 (MQ=255)                                    aGGTATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGGATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGTTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGata                                                                                               <  1:157734/140‑1 (MQ=255)                                    aGGTATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGGATAAACGAAATCTGATATACATTTATGCTATTGGACTTAATCTGATAGGTTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGata                                                                                               <  1:187251/140‑1 (MQ=255)                                       tATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGGATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGTTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTg                                                                                            <  2:515346/140‑1 (MQ=255)                                         ttCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGGATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGTTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCaga                                                                                                    <  2:137586/130‑1 (MQ=255)                                         ttCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGGATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGTTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCaga                                                                                                    >  1:137586/1‑130 (MQ=255)                                         ttCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGGATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGTTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGgt                                                                                          >  1:471210/1‑140 (MQ=255)                                         ttCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGGATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGTTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGgt                                                                                          >  2:455995/1‑140 (MQ=255)                                         ttCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGGATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGTTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGgt                                                                                          >  2:39592/1‑140 (MQ=255)                                              aTGATTCCTGATTATTTAACTTTTATTCGCTTTCAGGATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGTTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGGTGtagt                                                                                     <  1:110624/140‑1 (MQ=255)                                                 aTTCCTGATTATTTAACTTTTATTCGCTTTCAGGATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGTTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGGTGtagttag                                                                                  >  1:341714/1‑140 (MQ=255)                                                  ttCCTGATTATTTAACTTTTATTCGCTTTCAGGATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGTTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGGTGtagttagt                                                                                 <  2:279220/140‑1 (MQ=255)                                                   tCCTGATTATTTAACTTTTATTCGCTTTCAGGATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGTTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGGTGTAGTTAGTg                                                                                >  1:519295/1‑140 (MQ=255)                                                   tCCTGATTATTTAACTTTTATTCGCTTTCAGGATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGTTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGGTGTAGTTAGTg                                                                                >  2:290783/1‑140 (MQ=255)                                                          tattTAACTTTTATTCGCTTTCAGGATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGTTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGGTGTAGTTAGTGGGATTCt                                                                         <  1:455995/140‑1 (MQ=255)                                                                  ttttATTCGCTTTCAGGATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGTTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGGTGTAGTTAGTGGGATTCTGGCTCTgg                                                                 >  2:240892/1‑140 (MQ=255)                                                                    ttATTCGCTTTCAGGATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGTTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGGTGTAGTTAGTGGGATTCTGGCTCTGGTg                                                               <  2:519295/140‑1 (MQ=255)                                                                    ttATTCGCTTTCAGGATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGTTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGGTGTAGTTAGTGGGATTCTGGCTCTGGTg                                                               <  1:39592/140‑1 (MQ=255)                                                                          gCTTTCAGGATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGTTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGGTGTAGTTAGTGGGATTCTGGCTCTGGTGCTGtat                                                         <  2:288008/140‑1 (MQ=255)                                                                                      aaCGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGTTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGGTGTAGTTAGTGGGATTCTGGCTCTGGTGCTGTATAATTTTAttttt                                             >  2:94600/1‑140 (MQ=255)                                                                                               tGATATACATTTATGCTATTGGACTTATTCTGATAGGTTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGGTGTAGTTAGTGGGATTCTGGCTCTGGTGCTGTATAATTTTATTTTTGATCTCAAg                                    <  2:341714/140‑1 (MQ=255)                                                                                                    taCATTTATGCTATTGGACTTATTCTGATAGGTTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGGTGTAGTTAGTGGGATTCTGGCTCTGGTGCTGTATAATTTTATTTTTGATCTCAAGGCGTa                               <  1:240892/140‑1 (MQ=255)                                                                                                      cATTTATGCTATTGGACTTATTCTGATAGGTTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGGTGTAGTTAGTGGGATTCTGGCTCTGGTGCTGTATAATTTTATTTTTGATCTCAAGGCGTACt                             <  2:471210/140‑1 (MQ=255)                                                                                                         ttATGCTATTGGACTTATTCTGATAGGTTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGGTGTAGTTAGTGGGATTCTGGCTCTGGTGCTGTATAATTTTATTTTTGATCTCAAGGCGTACTggg                          >  2:500839/1‑140 (MQ=255)                                                                                                           aTGCTATTGGACTTATTCTGATAGGTTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGGTGTAGTTAGTGGGATTCTGGCTCTGGTGCTGTATAATTTTATTTTTGATCTCAAg                                    <  2:89273/128‑1 (MQ=255)                                                                                                           aTGCTATTGGACTTATTCTGATAGGTTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGGTGTAGTTAGTGGGATTCTGGCTCTGGTGCTGTATAATTTTATTTTTGATCTCAAg                                    >  1:89273/1‑128 (MQ=255)                                                                                                            tGCTATTGGACTTATTCTGATAGGTTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGGTGTAGTTAGTGGGATTCTGGCTCTGGTGCTGTATAATTTTATTTTTGATCTCAAGGCGTACTGGGCtt                       >  2:252436/1‑140 (MQ=255)                                                                                                                 ttGGACTTATTCTGATAGGTTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGGTGTAGTTAGTGGGATTCTGGCTCTGGTGCTGTATAATTTTATTTTTGATCTCAAGGCGTACTGGGCTTATaaa                  >  1:66005/1‑140 (MQ=255)                                                                                                                   ggACTTATTCTGATAGGTTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGGTGTAGTTAGTGGGATTCTGGCTCTGGTGCTGTATAATTTTATTTTTGATCTCAAGGCGTACTGGGCTTATAAATg                >  2:291529/1‑140 (MQ=255)                                                                                                                         aTTCTGATAGGTTTTTATTGGAAGAATGCAGGGTTTAC‑TTTCCATCAGAGGATATTGGTGTAGTTAGTGGGATTCTGGCTCTGGTGCTGTATAATTTTATTTTTGATCTCAAGGCGTACTGGGCTTATAAATGCGTCACg         <  1:331396/140‑1 (MQ=255)                                                                                                                          ttCTGATAGGTTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGGTGTAGTTAGTGGGATTCTGGCTCTGGTGCTGTATAATTTTATTTTTGATCTCAAGGCGTACTGGGCTTATAAATGCGTCACg         <  2:329457/140‑1 (MQ=255)                                                                                                                           tCTGATAGGTTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGGTGTAGTTAGTGGGATTCTGGCTCTGGTGCTGTATAATTTTATTTTTGATCTCAAGGCGTACTGGGCTTATAAATGCGTCACga        >  1:44276/1‑140 (MQ=255)                                                                                                                                 aGGTTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGGTGTAGTTAGTGGGATTCTGGCTCTGGTGCTGTATAATTTTATTTTTGATCTCAAGGCGTACTGGGCTTATAAATGCGTCACGAAGAata  >  2:336100/1‑140 (MQ=255)                                                                                                                                 aGGTTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGGTGTAGTTAGTGGGATTCTGGCTCTGGTGCTGTATAATTTTATTTTTGATCTCAAGGCGTACTGGGCTTATAAATGCGTCACGAAGAata  <  1:16355/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                         AGTTATGCATTGTCGTAAAGCATTTAGTGATGCAAAGGTATTCAAATGATTCCTGATTATTTAACTTTTATTCGCTTTCAGGATAAACGAAATCTGATATACATTTATGCTATTGGACTTATTCTGATAGGCTTTTATTGGAAGAATGCAGGGTTTACTTTTCCATCAGAGGATATTGGTGTAGTTAGTGGGATTCTGGCTCTGGTGCTGTATAATTTTATTTTTGATCTCAAGGCGTACTGGGCTTATAAATGCGTCACGAAGAATA  >  NC_000913/1095798‑1096065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑