Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	211,834	C→A	intergenic (+14/‑43)	ldcC → / → yaeR	lysine decarboxylase 2/VOC domain‑containing protein YaeR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	211,834	0	C	A	100.0%	151.5 / NA	47	intergenic (+14/‑43)	ldcC/yaeR	lysine decarboxylase 2/VOC domain‑containing protein YaeR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (22/25);  total (22/25)

GATTTTCTACTGATGCTTTGTTCCGTCGGGCAACATTACCCCGGTTTTGAAACGGATATTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGCTTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTATGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGCGACGGATTATGCGGTGAGCAAAGCTTTCTACTGCGATATTCTTGGTTTCACG  >  NC_000913/211698‑211969                                                                                                                                         |                                                                                                                                        gATTTTCTACTGATGCTTTGTTCCGTCGGGCAACATTACCCCGGTTTTGAAACGGATATTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGATTc                                                                                                                                      <  2:83919/140‑1 (MQ=255)    tttCTACTGATGCTTTGTTCCGTCGGGCAACATTACCCCGGTTTTGAAACGGATATTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGATTCCgg                                                                                                                                   <  2:743429/140‑1 (MQ=255)      tCTACTGATGCTTTGTTCCGTCGGGCAACATTACCCCGGTTTTGAAACGGATATTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGATTCCgggg                                                                                                                                 >  2:632069/1‑139 (MQ=255)      tCTACTGATGCTTTGTTCCGTCGGGCAACATTACCCCGGTTTTGAAACGGATATTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGATTCCGGGc                                                                                                                                 >  2:428858/1‑140 (MQ=255)      tCTACTGATGCTTTGTTCCGTCGGGCAACATTACCCCGGTTTTGAAACGGATATTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGATTCCGGGc                                                                                                                                 >  1:637473/1‑140 (MQ=255)      tCTACTGATGCTTTGTTCCGTCGGGCAACATTACCCCGGTTTTGAAACGGATATTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGATTCCGGGc                                                                                                                                 >  1:749224/1‑140 (MQ=255)      tCTACTGATGCTTTGTTCCGTCGGGCAACATTACCCCGGTTTTGAAACGGATATTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGATTCCGGGc                                                                                                                                 >  1:280866/1‑140 (MQ=255)      tCTACTGATGCTTTGTTCCGTCGGGCAACATTACCCCGGTTTTGAAACGGATATTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGATTCCGGGc                                                                                                                                 >  2:686789/1‑140 (MQ=255)      tCTACTGATGCTTTGTTCCGTCGGGCAACATTACCCCGGTTTTGAAACGGATATTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGATTCCGGGc                                                                                                                                 >  2:70240/1‑140 (MQ=255)             aTGCTTTGTTCCGTCGGGCAACATTACCCCGGTTTTGAAACGGATATTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGATTCCGGGCGGGTaaa                                                                                                                          >  2:104512/1‑139 (MQ=255)             aTGCTTTGTTCCGTCGGGCAACATTACCCCGGTTTTGAAACGGATATTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGATTCCGGGCGAGTAAc                                                                                                                          >  2:684137/1‑140 (MQ=255)                cTTTGTTCCGTCGGGCAACATTACCCCGGTTTTGAAACGGATATTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGATTCCGGGCGAGTAACGTg                                                                                                                       <  1:632069/140‑1 (MQ=255)                    gTTCCGTCGGGCAACATTACCCCGGTTTTGAAACGGATATTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCT‑AAAATGGCGGGATAACTTGCCAGAGCGGATTCCGGGCGAGTAACGTGCTGtt                                                                                                                  <  1:291019/140‑1 (MQ=255)                      tCCGTCGGGCAACATTACCCCGGTTTTGAAACGGATATTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGATTCCGGGCGAGTAACGTGCTGTTa                                                                                                                 <  2:280866/140‑1 (MQ=255)                         gTCGGGCAACATTACCCCGGTTTTGAAACGGATATTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGATTCCGGGCGAGTAACGTGCTGTTaa                                                                                                                >  2:464900/1‑138 (MQ=255)                         gTCGGGCAACATTACCCCGGTTTTGAAACGGATATTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGATTCCGGGCGAGTAACGTGCTGTTaa                                                                                                                <  1:464900/138‑1 (MQ=255)                           cGGGCAACATTACCCCGGTTTTGAAACGGATATTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGATTCCGGGCGAGTAACGTGCTGTTAACaaa                                                                                                            <  1:686789/140‑1 (MQ=255)                              gCAACATTACCCCGGTTTTGAAACGGATATTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGATTCCGGGCGAGTAACGTGCTGTTAACAAATaa                                                                                                         <  2:159122/140‑1 (MQ=255)                                 aCATTACCCCGGTTTTGAAACGGATATTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTGAAAATGGCGGGATAACTTGCCAGAGCGGATTCCGGGCGAGTAACGTGCTGTTAACAAATAAAgg                                                                                                      <  1:736990/140‑1 (MQ=255)                                        cccGGTTTTGAAACGGATATTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGATTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGtt                                                                                               >  1:156461/1‑140 (MQ=255)                                           ggTTTTGAAACGGATATTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGATTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTATg                                                                                            <  1:70240/140‑1 (MQ=255)                                           ggTTTTGAAACGGATATTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGATTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTATg                                                                                            <  1:428858/140‑1 (MQ=255)                                            gTTTTGAAACGGATATTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGATTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTATGc                                                                                           >  1:309175/1‑140 (MQ=255)                                            gTTTTGAAACGGATATTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGATTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTATGc                                                                                           >  2:259190/1‑140 (MQ=255)                                            gTTTTGAAACGGATATTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGATTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTATGc                                                                                           >  2:143711/1‑140 (MQ=255)                                             ttttGAAACGGATATTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGATTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTATGCt                                                                                          <  2:156461/140‑1 (MQ=255)                                                      ggATATTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGATTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTATGCTGGGTTTaaa                                                                                 >  1:260268/1‑140 (MQ=255)                                                       gATATTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGATTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTATGCTGGGTTTaaaa                                                                                <  1:684137/140‑1 (MQ=255)                                                        atatTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGATTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTATGCTGGGTTTAAAAc                                                                               <  2:637473/140‑1 (MQ=255)                                                        atatTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGATTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTATGCTGGGTTTAAAAc                                                                               <  2:309175/140‑1 (MQ=255)                                                                ggCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGATTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTATGCTGGGTTTAAAACAGGTTCAc                                                                       >  2:18459/1‑140 (MQ=255)                                                                 gcgcGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGATTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTATGCTGGGTTTAAAACAGGTTCAcc                                                                      <  2:303069/140‑1 (MQ=255)                                                                  cgcgAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGATTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTATGCTGGGTTTAAAACAGGTTCAccc                                                                     >  1:201655/1‑139 (MQ=255)                                                                    cgAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGATTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTATGCTGGGTTTAAAACAGGTTCACCata                                                                   >  1:298540/1‑140 (MQ=255)                                                                             aCGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGATTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTATGCTGGGTTTAAAACAGGTTCACCATATTGCGatta                                                          <  2:260268/140‑1 (MQ=255)                                                                                 aGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGATTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTATGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGc                                                      >  1:225685/1‑140 (MQ=255)                                                                                  gACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGATTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTATGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGCg                                                     <  1:259190/140‑1 (MQ=255)                                                                                  gACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGATTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTATGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGCg                                                     <  1:143711/140‑1 (MQ=255)                                                                                    cGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGATTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTATGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGCGAc                                                   <  2:225685/140‑1 (MQ=255)                                                                                      gCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGTCAGAGCGGATTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTATGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGCGACgg                                                 >  1:54953/1‑140 (MQ=255)                                                                                                   aCGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGATTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTATGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGCGACGGATTATGCGGTGAg                                    <  2:708490/140‑1 (MQ=255)                                                                                                       gTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGATTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTATGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGCGACGGATTATGCGGTGAGCaaa                                >  1:654059/1‑140 (MQ=255)                                                                                                                   gCGGGATAACTTGCCAGAGCGGATTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTATGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGCGACGGATTATGCGGTGAGCa                                  >  2:394081/1‑126 (MQ=255)                                                                                                                   gCGGGATAACTTGCCAGAGCGGATTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTATGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGCGACGGATTATGCGGTGAGCa                                  <  1:394081/126‑1 (MQ=255)                                                                                                                    cGGGATAACTTGTCAGAGCGGATTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTATGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGCGACGGATTATGCGGTGAGCAAAGCTTTCTACTGCg                   <  2:54953/140‑1 (MQ=255)                                                                                                                    cGGGATAACTTGCCAGAGCGGATTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTATGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGCGACGGATTATGCGGTGAGCAAAGCTTTCTACTGCg                   >  2:42898/1‑140 (MQ=255)                                                                                                                    cGGGATAACTTGCCAGAGCGGATTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTATGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGCGACGGATTATGCGGTGAGCAAAGCTTTCTACTGCg                   >  1:434274/1‑140 (MQ=255)                                                                                                                    cGGGATAACTTGCCAGAGCGGATTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTATGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGCGACGGATTATGCGGTGAGCAAAGCTTTCTACTGCg                   <  1:18459/140‑1 (MQ=255)                                                                                                                                ccAGAGCGGATTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTATGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGCGACGGATTATGCGGTGAGCAAAGCTTTCTACTGCGATATTCTTGGtt       >  2:137878/1‑140 (MQ=255)                                                                                                                                   gagCGGATTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTATGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGCGACGGATTATGCGGTGAGCAAAGCTTTCTACTGCGATATTCTTGGTTTCa    >  2:61637/1‑140 (MQ=255)                                                                                                                                    agCGGATTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTATGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGCGACGGATTATGCGGTGAGCAAAGCTTTCTACTGCGATATTCTTGGTTTCAc   >  2:724287/1‑140 (MQ=255)                                                                                                                                     gCGGATTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTATGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGCGACGGATTATGCGGTGAGCAAAGCTTTCTACTGCGATATTCTTGGTTTCACg  <  2:749224/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        GATTTTCTACTGATGCTTTGTTCCGTCGGGCAACATTACCCCGGTTTTGAAACGGATATTCACGGCGCGAAACAGGACGAAGACGGCGTTTACCGCGTACGAGTCCTAAAAATGGCGGGATAACTTGCCAGAGCGGCTTCCGGGCGAGTAACGTGCTGTTAACAAATAAAGGAGACGTTATGCTGGGTTTAAAACAGGTTCACCATATTGCGATTATTGCGACGGATTATGCGGTGAGCAAAGCTTTCTACTGCGATATTCTTGGTTTCACG  >  NC_000913/211698‑211969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑