Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,354,144	G→T	G125G (GGC→GGA)	sapC ←	putrescine ABC exporter membrane protein SapC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,354,144	0	G	T	100.0%	143.3 / NA	41	G125G (GGC→GGA)	sapC	putrescine ABC exporter membrane protein SapC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (18/23);  total (18/23)

AGCAATGCCAGCCAGACAGCAAACATAGCGTGGGACAAACTCGGTCCGGCAAACGCTACAACGATAATTGCCAACAGCAGCGAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCA  >  NC_000913/1354010‑1354279                                                                                                                                       |                                                                                                                                        agcaATGCCAGCCAGACAGCAAACATAGCGTGGGACAAACTCGGTCCGGCAAACGCTACAACGATAATTGCCAACAGCAGCGAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGTCCgtg                                                                                                                                    >  1:502729/1‑140 (MQ=255)          agccagACAGCAAACATAGCGTGGGACAAACTCGGTCCGGCAAACGCTACAACGATAATTGCCAACAGCAGCGAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGTCCGTGTGTCGc                                                                                                                              <  1:8805/137‑1 (MQ=255)          agccagACAGCAAACATAGCGTGGGACAAACTCGGTCCGGCAAACGCTACAACGATAATTGCCAACAGCAGCGAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGTCCGTGTGTCGc                                                                                                                              >  2:8805/1‑137 (MQ=255)             cagACAGCAAACATAGCGTGGGACAAACTCGGTCCGGCAAACGCTACAACGATAATTGCCAACAGCAGCGAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGTCCGTGTGTCGCCCCGGc                                                                                                                        >  2:666340/1‑140 (MQ=255)              agACAGCAAACATAGCGTGGGACAAACTCGGTCCGGCAAACGCTACAACGATAATTGCCAACAGCAGCGAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGTCCGTGTGTCGCCCCGGCg                                                                                                                       <  2:502729/140‑1 (MQ=255)               gACAGCAAACATAGCGTGGGACAAACTCGGTCCGGCAAACGCTACAACGATAATTGCCAACAGCAGCGAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGTCCGTGTGTCGCCCCGGCGa                                                                                                                      <  2:322602/140‑1 (MQ=255)                 cAGCAAACATAGCGTGGGACAAACTCGGTCCGGCAAACGCTACAACGATAATTGCCAACAGCAGCGAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGTCCGTGTGTCGCCCCGGCGAAg                                                                                                                    <  2:23891/140‑1 (MQ=255)                            gCGTGGGACAAACTCGGTCCGGCAAACGCTACAACGATAATTGCCAACAGCAGCGAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGTCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTAc                                                                                                         <  1:99873/140‑1 (MQ=255)                             cGTGGGACAAACTCGGTCCGGCAAACGCTACAACGATAATTGCCAACAGCAGCGAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGTCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTAcc                                                                                                        <  1:114548/140‑1 (MQ=255)                              gTGGGACAAACTCGGTCCGGCAAACGCTACAACGATAATTGCCAACAGCAGCGAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGTCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCa                                                                                                       <  2:247123/140‑1 (MQ=255)                              gTGGGACAAACTCGGTCCGGCAAACGCTACAACGATAATTGCCAACAGCAGCGAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGTCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCa                                                                                                       >  1:247123/1‑140 (MQ=255)                                       aCTCGGTCCGGCAAACGCTACAACGATAATTGCCAACAGCAGCGAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGTCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGa                                                                                              >  1:192722/1‑140 (MQ=255)                                                 gCAAACGCTACAACGATAATTGCCAACAGCAGCGAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGTCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGc                                                                                    >  1:529285/1‑140 (MQ=255)                                                     aCGCTACAACGATAATTGCCAACAGCAGCGAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGTCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGc                                                                                <  1:396276/140‑1 (MQ=255)                                                                aTAATTGCCAACAGCAGCGAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGTCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACaaa                                                                     >  1:288280/1‑140 (MQ=255)                                                                   aTTGCCAACAGCAGCGAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGTCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATgc                                                                  >  1:527953/1‑140 (MQ=255)                                                                        cAACAGCAGCGAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGTCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGccac                                                             >  1:704121/1‑140 (MQ=255)                                                                                 cGAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGTCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTg                                                    >  2:341259/1‑140 (MQ=255)                                                                                    aGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGTCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGgcg                                                 <  1:628179/140‑1 (MQ=255)                                                                                    aGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGTCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGgcg                                                 <  1:419621/140‑1 (MQ=255)                                                                                         tCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGTCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCt                                            <  2:529285/140‑1 (MQ=255)                                                                                            ccAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGTCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCg                                         <  1:482190/140‑1 (MQ=255)                                                                                             cAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGTCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGc                                        <  2:192722/140‑1 (MQ=255)                                                                                              aGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGTCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCt                                       <  2:527953/140‑1 (MQ=255)                                                                                                  aGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGTCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGt                                   >  2:84057/1‑140 (MQ=255)                                                                                                     tATCCAGAATATGGTTAAGCACCGCTGAGCGGAGTCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTaaa                                <  2:704121/140‑1 (MQ=255)                                                                                                       tCCAGAATATGGTTAAGCACCGCTGAGCGGAGTCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACg                              <  1:666340/140‑1 (MQ=255)                                                                                                                 ggTTAAGCACCGCTGAGCGGAGTCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACa                    >  2:695079/1‑140 (MQ=255)                                                                                                                 ggTTAAGCACCGCTGAGCGGAGTCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACa                    >  1:449895/1‑140 (MQ=255)                                                                                                                  gTTAAGCACCGCTGAGCGGAGTCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACAt                   <  1:592770/140‑1 (MQ=255)                                                                                                                  gTTAAGCACCGCTGAGCGGAGTCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGATCCGCTCAGTAAACGGCTTAAAACAt                   <  1:348539/140‑1 (MQ=255)                                                                                                                   ttAAGCACCGCTGAGCGGAGTCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATc                  >  2:534581/1‑140 (MQ=255)                                                                                                                        cACCGCTGAGCGGAGTCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTc             >  1:187115/1‑140 (MQ=255)                                                                                                                         aCCGCTGAGCGGAGTCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTcc            <  1:695079/140‑1 (MQ=255)                                                                                                                           cGCTGAGCGGAGTCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGa          >  1:365896/1‑140 (MQ=255)                                                                                                                            gCTGAGCGGAGTCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAg         <  2:288280/140‑1 (MQ=255)                                                                                                                            gCTGAGCGGAGTCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAg         >  2:678843/1‑140 (MQ=255)                                                                                                                            gCTGAGCGGAGTCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAg         <  1:84057/140‑1 (MQ=255)                                                                                                                                aGCGGAGTCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGgtcg     >  1:519180/1‑140 (MQ=255)                                                                                                                                 gCGGAGTCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGgtcgt    <  2:519180/140‑1 (MQ=255)                                                                                                                                   ggAGTCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCa  <  2:449895/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        AGCAATGCCAGCCAGACAGCAAACATAGCGTGGGACAAACTCGGTCCGGCAAACGCTACAACGATAATTGCCAACAGCAGCGAAGGGATCGCCAGTAAGGTATCCAGAATATGGTTAAGCACCGCTGAGCGGAGGCCGTGTGTCGCCCCGGCGAAGGTGCCCAGTACCAGGCCACAGATCGTCGCGGCAAGCGTCACCACAAATGCGCCACCCACGGTTGGCGCAGCTCCGCTCAGTAAACGGCTTAAAACATCGCGTCCGAGGTCGTCA  >  NC_000913/1354010‑1354279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑