Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,337,694	T→C	D576G (GAC→GGC)	gyrA ←	DNA gyrase subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,337,694	0	T	C	100.0%	44.8 / NA	13	D576G (GAC→GGC)	gyrA	DNA gyrase subunit A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/1);  total (12/1)

TTGACGATCGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGC‑AGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTT‑CCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGA  >  NC_000913/2337559‑2337825                                                                                                                                         |                                                                                                                                     ttGACGATCGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGC‑AGTCGGTCGATAAAGccttc                                                                                                                                  <  1:604256/140‑1 (MQ=255) ttGACGATCGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGC‑AGTCGGTCGATAAAGccttc                                                                                                                                  <  2:134765/140‑1 (MQ=255) ttGACGATCGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGC‑AGTCGGTCGATAAAGcctt                                                                                                                                   >  2:357544/1‑139 (MQ=255) ttGACGATCGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGC‑AGTCGGTCGATAAAGcctt                                                                                                                                   <  1:357544/139‑1 (MQ=255)  tGACGATCGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGC‑AGTCGGTCGATAAAGccttct                                                                                                                                 <  2:379605/140‑1 (MQ=255)  tGACGATCGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGC‑AGTCGGTCGATAAAGccttct                                                                                                                                 <  2:242130/140‑1 (MQ=255)        tCGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGC‑AGTCGGTCGATAAAGcctt                                                                                                                                   >  2:738956/1‑132 (MQ=255)        tCGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGC‑AGTCGGTCGATAAAGcctt                                                                                                                                   <  1:738956/132‑1 (MQ=255)             cgacCGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGC‑AGTCGGTCGATAAAGCCTTCTTCTTTAATACg                                                                                                                      <  2:689604/140‑1 (MQ=255)                                  gCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCGGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGC‑AGTCGGTCGATAAAGCCTTCTTCTTTAATACGTGCGGCAGATTTACCTTT‑ccc                                                                                                >  1:748499/1‑140 (MQ=255)                                    ttCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGC‑AGTCGGTCGATAAAGCCTTCTTCTTTAATACGTGCGGCAGATTTACCTTT‑CCCGc                                                                                              >  1:349789/1‑140 (MQ=255)                                       cGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGC‑AGTCGGTCGATAAAGCCTTCTTCTTTAATACGTGCGGCAGATTTACCTTT‑CCCGCCac                                                                                           >  2:371289/1‑140 (MQ=255)                                                  aaaCTTTCATCGAATAGCCGCGCCCACGGCGGGAGAAGCACAGAATTTGGTCGTGAGTGTCCGCCCCCAGC‑AGTCGGTCAATAAACCCTTCTTTTTTAATACGTGCGG‑AAATTTTCCTTTCCCCGCCACGACGCTGCGCt                                                                                <  2:487137/140‑1 (MQ=255)                                                          aTCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGC‑AGTCGGTCGATAAAGCCTTCTTCTTTAATACGTGCGGCAGATTTACCTTT‑CCCGCCACGACGCTGCGCTTCGTATTc                                                                        >  1:638442/1‑140 (MQ=255)                                                               aTAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGC‑AGTCGGTCGATAAAGCCTTCTTCTTTAATACGTGCGGCAGATTTACCTTT‑CCCGCCACGACGCTGCGCTTCGTATTCAGaaa                                                                   >  1:271188/1‑140 (MQ=255)                                                                      cgccccgggCTGGGGAAGCAAAGAATGTGGTGGGGAGTGTCCGCCCCCAGC‑ATTCGGTCAATAAAGCTTTCTTTTTTAATGGGTGCGGCAGATTTCCCTTT‑CCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTg                                                            <  2:324587/133‑1 (MQ=255)                                                                                       gCACAGAATATGGTCGTGAGTGTTCGCCACCAGC‑AGTCGGTCGATAAAGCCTTCTTCTTTAATACGTGCGGCAGATTTACCTTT‑CCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCt                                           >  2:290095/1‑140 (MQ=255)                                                                                       gCACAGAATATGGTCGTGAGTGTTCGCCACCAGC‑AGTCGGTCGATAAAGCCTTCTTCTTTAATACGTGCGGCAGATTTACCTTT‑CCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCt                                           >  1:176886/1‑140 (MQ=255)                                                                                                ttggtggTGAGT‑TTCCCCCCCAGCGCGTCGGTCGATAAACCCTTCTTCTTTAGTGTTTGCGGGAGTTTTCCCTTG‑CCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTgagaga                                  <  2:371421/139‑1 (MQ=255)                                                                                                         aGTGTTCGCCACCAGC‑AGTCGGTCGATAAAGCCTTCTTCTTTAATACGTGCGGCAGATTTACCTTT‑CCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCACTGGTGAGAGAGCGTCACGa                         >  2:323416/1‑140 (MQ=255)                                                                                                             tccccccccaGC‑AGTCGGTCGATAAACCCTTTTTTTTTAATACGTGCGGCAGTTTTCCTTTT‑CCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACcac                     <  2:638442/133‑1 (MQ=255)                                                                                                               cccccccaGC‑AGTCGGTCAAAAAACCCTTCTTTTTAAATACGTGCGGCAGTTTTCCCTTT‑CCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACAt                   <  2:271188/135‑1 (MQ=255)                                                                                                                gCCACCAGC‑AGTCGGTCGATAAAGCCTTCTTCTTTAATACGTGCGGCAGATTTACCTTT‑CCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATc                  >  1:72440/1‑140 (MQ=255)                                                                                                                cccaccaGC‑AGTCGGTCAAAAAACCTTTTTTTTTTAATGCGTGCGGCAGATTTCCTTTC‑CCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATc                  <  2:543901/139‑1 (MQ=255)                                                                                                                     cagc‑agTCGGTCGATAAAGCCTTCTTCTTTAATACGTGCGGCAGATTTACCTTT‑CCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCt             >  2:32708/1‑140 (MQ=255)                                                                                                                           gtcggtcaAAAAACCCTTCTTCTTAAATGCGTGCGGAATTTTTCCTTTT‑CCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTg        <  2:349789/140‑1 (MQ=255)                                                                                                                            tcggtcgATAAAGCCTTCTTTTTTAATGCGTGCGGAAGTTTTCCCTTC‑CCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGa       <  1:601810/140‑1 (MQ=255)                                                                                                                            tcggtcgATAAAGCCTTCTTCTTTAATACGTGCGGCAGATTTACCTTT‑CCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGa       >  1:543901/1‑140 (MQ=255)                                                                                                                                tcgATAAAGCTTTCTTTTTTAATACGTGCGGCAGATTTCCCTTT‑CCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAg   <  1:107058/140‑1 (MQ=255)                                                                                                                                 cgATAAAGCCTTCTTCTTTAATACGTGCGGCAGATTTACCTTT‑CCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGa  >  2:534211/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     TTGACGATCGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGC‑AGTCGGTCGATAAAGTCTTCTTCTTTAATACGTGCGGCAGATTTACCTTT‑CCCGCCACGACGCTGCGCTTCGTATTCAGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGA  >  NC_000913/2337559‑2337825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑