Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,817,985	G→T	intergenic (‑125/+74)	argQ ← / ← argZ	tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,817,985	0	G	T	95.6%	138.1 / 0.1	45	intergenic (‑125/+74)	argQ/argZ	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (19/24);  total (20/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.28e-01

GAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGC  >  NC_000913/2817848‑2818121                                                                                                                                          |                                                                                                                                         gAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACttt                                                                                                                                        <  1:192555/140‑1 (MQ=255)  aGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTa                                                                                                                                       >  2:422892/1‑140 (MQ=255)  aGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTa                                                                                                                                       >  1:48077/1‑140 (MQ=255)  aGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCCCGCCCTTCGGGCCGATACTAAAGCAACTTTa                                                                                                                                       >  1:74909/1‑140 (MQ=255)   gCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTat                                                                                                                                      <  1:325936/140‑1 (MQ=255)    ttACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTatc                                                                                                                                     >  2:216662/2‑140 (MQ=255)    cTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTatc                                                                                                                                     >  1:52557/1‑140 (MQ=255)     tACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTatca                                                                                                                                    <  1:490861/140‑1 (MQ=255)                 gggAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTATCATCCCTAGTGCCt                                                                                                                        >  1:11901/1‑140 (MQ=255)                      aaCTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGt                                                                                                                   <  1:422892/140‑1 (MQ=255)                        cTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGTTa                                                                                                                 >  1:547044/1‑140 (MQ=255)                            ttCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGTTAATTc                                                                                                             >  2:365253/1‑140 (MQ=255)                               tACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGTTAATTCCGc                                                                                                          <  1:264908/140‑1 (MQ=255)                                aCTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGTTAATTCCGCt                                                                                                         <  1:442515/140‑1 (MQ=255)                                  tgctgATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTAc                                                                                                       >  2:164346/1‑140 (MQ=255)                                   gctgATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTAcc                                                                                                      >  1:707943/1‑140 (MQ=255)                                   gctgATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTAcc                                                                                                      <  1:216662/140‑1 (MQ=255)                                    ctgATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTTTCATCCCCAGTGCCCGCGGGTAATTCCGCTAccc                                                                                                     >  2:264908/1‑139 (MQ=17)                                       atatTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGACCTTCGGGCCGGTGCTTAATAAACCTTTTCATCCCTAGCGCCTGGCGGTAATTCCCCTACCCCtt                                                                                                  >  2:650888/1‑140 (MQ=14)                                              tACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATAcc                                                                                           >  2:256003/1‑140 (MQ=255)                                              tACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGTTAATTCCACTACCTCTTCGATAcc                                                                                           >  2:643979/1‑140 (MQ=255)                                                ccGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTTTCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCtt                                                                                         >  2:450253/1‑140 (MQ=255)                                                ccGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCtt                                                                                         <  2:11901/140‑1 (MQ=255)                                                 cGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTc                                                                                        >  2:402702/1‑140 (MQ=255)                                                   ctactaAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACctct                                                                                                   >  2:249966/1‑127 (MQ=255)                                                   ctactaAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACctct                                                                                                   <  1:249966/127‑1 (MQ=255)                                                   ctactaAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCAt                                                                                      >  1:108450/1‑140 (MQ=255)                                                   ctactaAAGCCATATCAATCAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCAt                                                                                      >  1:186420/1‑140 (MQ=255)                                                              atatCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGAtt                                                                           <  1:63858/140‑1 (MQ=255)                                                                atCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTc                                                                         >  1:396901/1‑140 (MQ=255)                                                                atCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTc                                                                         >  2:306566/1‑140 (MQ=255)                                                                atCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTc                                                                         >  2:327659/1‑140 (MQ=255)                                                                atCAATTAAGAGATCGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGGTGCTACAGCAACTTTATCATCACTAGGGCCTGCGGTTTGATCCCCCACCTCCTCGAAACCTTAATTGTCGAAGActtt                                                                         >  2:449203/1‑139 (MQ=14)                                                                   aaTTAAGAGATGGTGCTTCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGga                                                                      <  2:259776/140‑1 (MQ=255)                                                                   aaTTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGTTAATTCCACTACCTCTTCGATACCTTCATTGCTGAAGATTTCGga                                                                      <  1:643979/140‑1 (MQ=255)                                                                   aaTCAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGga                                                                      <  2:186420/140‑1 (MQ=255)                                                                     ttAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGgaga                                                                    <  1:164346/140‑1 (MQ=255)                                                                      tAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGaa                                                                   <  1:327659/140‑1 (MQ=255)                                                                      tAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGaa                                                                   >  1:287128/1‑140 (MQ=255)                                                                           gaTGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATgg                                                              <  1:256003/140‑1 (MQ=255)                                                                             tGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTg                                                            <  1:365253/140‑1 (MQ=255)                                                                                tGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTCCCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGcct                                                         <  2:108450/140‑3 (MQ=255)                                                                                  cATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATcc                                                       <  2:48077/140‑1 (MQ=255)                                                                                    tCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCgg                                                     >  2:372974/1‑140 (MQ=255)                                                                                                      cTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGa                                   >  2:599544/1‑140 (MQ=255)                                                                                                           ggCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCt                              <  1:306566/140‑1 (MQ=255)                                                                                                           ggCTCGCCCTTCGGGCCGTTGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCt                              <  1:650888/140‑1 (MQ=255)                                                                                                                      cGGGCCGTTGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGc                   >  2:423109/1‑140 (MQ=255)                                                                                                                         gCCGTTGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGa                <  1:372974/140‑1 (MQ=255)                                                                                                                            gTTGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTa             <  2:52557/140‑1 (MQ=255)                                                                                                                            gTTGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTa             <  2:707943/140‑1 (MQ=255)                                                                                                                            gTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTa             >  2:578116/1‑140 (MQ=255)                                                                                                                              tGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGcc                  >  2:747496/1‑133 (MQ=255)                                                                                                                              tGCTAAAGCAACTTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGcc                  <  1:747496/133‑1 (MQ=255)                                                                                                                                       atcGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGc  <  1:578116/138‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         GAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGC  >  NC_000913/2817848‑2818121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑