Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,479,225 A→T G461G (GGT→GGA)  kefB ← K(+) : H(+) antiporter KefB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,479,2250AT97.6% 136.7 / NA 41G461G (GGT→GGA) kefBK(+) : H(+) antiporter KefB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (16/24);  minor base C (0/1);  total (16/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCATGCGCTTCCACACGTCCGCGCGCTCGCGCAAGAATATGCAAATGCGGAAAGTGCTGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGAACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGATATCCCGCTCCAGCACGGTAATGCGCATTTTATTTGCCATCAGCAAACG  >  NC_000913/3479090‑3479359
                                                                                                                                       |                                                                                                                                      
cTCATGCGCTTCCACACGTCCGCGCGCTCGCGCAAGAATATGCAAATGCGGAAAGTGCTGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCTCCCg                                                                                                                                    <  1:200647/140‑1 (MQ=255)
cTCATGCGCTTCCACACGTCCGCGCGCTCGCGCAAGAATATGCAAATGCGGAAAGTGCTGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCTCCCg                                                                                                                                    <  2:140995/140‑1 (MQ=255)
  cATGCGCTTCCACACGTCCGCGCGCTCGCGCAAGAATATGCAAATGCGGAAAGTGCTGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCTCCCGCa                                                                                                                                  <  1:357161/140‑1 (MQ=255)
    tGCGCTTCCACACGTCCGCGCGCTCGCGCAAGAATATGCAAATGCGGAAAGTGCTGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCTCCCGCAGa                                                                                                                                >  2:686961/1‑140 (MQ=255)
        cTTCCACACGTCCGCGCGCTCGCGCAAGAATATGCAAATGCGGAAAGTGCTGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCTTCCGCAGAACGt                                                                                                                            <  2:91668/140‑1 (MQ=255)
         ttCCACACGTCCGCGCGCTCGCGCAAGAATATGCAAATGCGGAAAGTGCTGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCTCCCGCAGAACGTa                                                                                                                           <  2:467399/140‑1 (MQ=255)
           ccACACGTCCGCGCGCTCGCGCAAGAATATGCAAATGCGGAAAGTGCTGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCTCCCGCAGAACGTaaa                                                                                                                         >  2:193869/1‑140 (MQ=255)
           ccACACGTCCGCGCGCTCGCGCAAGAATATGCAAATGCGGAAAGTGCTGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCTCCCGCAGAACGTa                                                                                                                           <  1:260905/138‑1 (MQ=255)
           ccACACGTCCGCGCGCTCGCGCAAGAATATGCAAATGCGGAAAGTGCTGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCTCCCGCAGAACGTa                                                                                                                           >  2:260905/1‑138 (MQ=255)
                   ccGCGCGCTCGCGCAAGAATATGCAAATGCGGAAAGTGCTGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCTCCCGCAGAACGTAAAAGATCGAc                                                                                                                 <  1:431042/140‑1 (MQ=255)
                   ccGCGCGCTCGCGCAAGAATATGCAAATGCGGAAAGTGCTGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCTCCCGCAGAACGTAAAAGATCGAc                                                                                                                 >  1:88696/1‑140 (MQ=255)
                             gcgcAAGAATATGCAAATGCGGAAAGTGCTGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTTCTCCCGCAGAACGTAAAAGATCGACCTGCGTGGCg                                                                                                       <  2:627678/140‑1 (MQ=255)
                                cAAGAATATGCAAATGCGGAAAGTGCTGTTGGCATATTTCCACCCGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCTCCCGCAGAACGTAAAAGATCGACCTGCGTGGCGTCg                                                                                                    <  1:197188/140‑1 (MQ=255)
                                cAAGAATATGCAAATGCGGAAAGTGCTGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCTCCCGCAGAACGTAAAAGATCGACCTGCGTGGCGTCg                                                                                                    <  2:602424/140‑1 (MQ=255)
                                cAAGAATATGCAAATGCGGAAAGTGCTGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCTCCCGCAGAACGTAAAAGATCGACCTGCGTGGCGTCg                                                                                                    <  1:686961/140‑1 (MQ=255)
                                        tGCAAATGCGGAAAGTGCTGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCTCCCGCAGAACGTAAAAGATCGACCTGCGTGGCGTCGCCGtaata                                                                                            <  2:130492/140‑1 (MQ=255)
                                             aTGCGGAAAGTGCTGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCTCCCGCAGAACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACtt                                                                                       >  2:389496/1‑140 (MQ=255)
                                              tGCGGAAAGTGCTGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCTCCCGCAGAACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACttt                                                                                      >  2:237800/1‑140 (MQ=255)
                                                   aaaGTGCTGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCTCCCGCAGAACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGc                                                                                 <  1:664911/140‑1 (MQ=255)
                                                   aaaGTGCTGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCTCCCGCAGAACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGc                                                                                 <  1:733391/140‑1 (MQ=255)
                                                           gTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCTCCCGCAGAACGTAAAAGATTGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTa                                                                         >  2:612924/1‑140 (MQ=255)
                                                             tGGCATATTTCCACTGCCTTCATGGTGCCGTCCGGCTCGTTACAGTTAATGACGATAGACTCAGCGGCGGCTGCTCCCGCTGAACCTAAAAGACCGTCCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACg                                                                       <  2:70772/140‑1 (MQ=255)
                                                                 atatTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCTCCCGCAGAACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATc                                                                   >  1:485669/1‑140 (MQ=255)
                                                                    tttCCACCAGCTTCATG‑‑GTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCTCCCGCAGAACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGtt                                                              >  1:265563/1‑140 (MQ=255)
                                                                        caccaGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCTCCCGCAGAACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTaa                                                            <  2:718856/140‑1 (MQ=255)
                                                                         accaGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCTCCCGCAGAACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCAt                                                                    >  2:413816/1‑131 (MQ=255)
                                                                         accaGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCTCCCGCAGAACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCAt                                                                    <  1:413816/131‑1 (MQ=255)
                                                                              tttCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCCCCCGCAGAACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGgcgc                                                      <  1:577257/139‑1 (MQ=255)
                                                                               ttCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCTCCCGCAGAACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCt                                                     <  1:363966/140‑1 (MQ=255)
                                                                                    ggTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCTCCCGCAGAACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGatat                                                <  2:171182/140‑1 (MQ=255)
                                                                                      tgtCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCTCCCGCAGAACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGATATcc                                              <  2:88696/140‑1 (MQ=255)
                                                                                                cTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCTCCCGCAGAACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGATATCCCGCTCCAGCa                                    <  1:193869/140‑1 (MQ=255)
                                                                                                cTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCTCCCGCAGAACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGATATCCCGCTCCAGCa                                    <  2:265563/140‑1 (MQ=255)
                                                                                                    ttACAGGTAATGACGATAGACTCAGCGGCCTCTGCTCCCGCAGAACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGATATCCCGCTc                                         >  1:297111/1‑131 (MQ=255)
                                                                                                    ttACAGGTAATGACGATAGACTCAGCGGCCTCTGCTCCCGCAGAACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGATATCCCGCTc                                         <  2:297111/131‑1 (MQ=255)
                                                                                                             aTGACGATAGACTCAGCGGCCTCTGCTCCCGCAGAACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGATATCCCGCTCCAGCACGGTAATGCGCAt                       >  1:503385/1‑140 (MQ=255)
                                                                                                                    tAGACTCAGCGGCCTCTGCTCCCGCAGAACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGATATCCCGCTCCAGCACGGTAATGCGCATTTTAttt                >  1:528180/1‑140 (MQ=255)
                                                                                                                    tAGACTCAGCGGCCTCTGCTCCCGCAGAACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGATATCCCGCTCCAGCACGGTAATGCGCATTTTAttt                >  2:373569/1‑140 (MQ=255)
                                                                                                                            gCGGCCTCTGCTCCCGCAGAACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGATATCCCGCTCCAGCACGGTAATGCGCATTTTATTTGCCATCAg        >  2:696346/1‑140 (MQ=255)
                                                                                                                              ggCCTCTGCTCCCGCAGAACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGATATCCCGCTCCAGCACGGTAATGCGCATTTTATTTGCCATCAGCa      <  1:373569/140‑1 (MQ=255)
                                                                                                                                 ctctGCTCCCGCAGAACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGATATCCCGCTCCAGCACGGTAATGCGCATTTTATTTGCCATCAGCAAAc   <  2:485669/140‑1 (MQ=255)
                                                                                                                                  tctGCTCCCGCAGAACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGATATCCCGCTCCAGCACGGTAATGCGCATTTTATTTGCCATCAGCAAACg  >  1:550543/1‑140 (MQ=255)
                                                                                                                                       |                                                                                                                                      
CTCATGCGCTTCCACACGTCCGCGCGCTCGCGCAAGAATATGCAAATGCGGAAAGTGCTGTTGGCATATTTCCACCAGCTTCATGGTGTCTTCCGGCTCGTTACAGGTAATGACGATAGACTCAGCGGCCTCTGCACCCGCAGAACGTAAAAGATCGACCTGCGTGGCGTCGCCGTAATAAACTTTGTAGCCGTATTTACGCATCAGGTTAACGGCGCTGATATCCCGCTCCAGCACGGTAATGCGCATTTTATTTGCCATCAGCAAACG  >  NC_000913/3479090‑3479359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑