Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,580,017	C→A	P263P (CCG→CCT)	yhhX ←	putative oxidoreductase YhhX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,580,017	0	C	A	100.0%	146.3 / NA	42	P263P (CCG→CCT)	yhhX	putative oxidoreductase YhhX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (22/20);  total (22/20)

GGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTCCAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCCGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATACCGTATTTAATAAACGAACCTTTCTTACCGTGAACGATAAATTTCGGATAATCGATTTTCACCAGATGGCTGGTTTTGACGATGGCTTTCAGGT  >  NC_000913/3579882‑3580153                                                                                                                                        |                                                                                                                                         ggTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTCCAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCAGGCa                                                                                                                                      <  2:73869/140‑1 (MQ=255) ggTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTCCAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCAGGCa                                                                                                                                      <  2:600403/140‑1 (MQ=255) ggTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTCCAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCAGGCa                                                                                                                                      <  2:275250/140‑1 (MQ=255) ggTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTCCAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCAGGCa                                                                                                                                      <  2:142083/140‑1 (MQ=255)   tttGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTCCAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCAGGCata                                                                                                                                    >  2:64209/1‑140 (MQ=255)     tGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTCCAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCAGGCataat                                                                                                                                  >  2:95045/1‑140 (MQ=255)      gATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTCCAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCAGGCataata                                                                                                                                 >  1:102232/1‑140 (MQ=255)             cGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTCCAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCAGGCATAATATTAGCCt                                                                                                                          >  1:548751/1‑140 (MQ=255)              gCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTCCGGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCAGGCATAATATTAGCCtt                                                                                                                         >  1:387579/1‑140 (MQ=255)                          cgcCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTCCAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCAGGCATAATATTAGCCTTCAGGCTGGTTTc                                                                                                             >  2:554558/1‑140 (MQ=255)                            cccGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTCCAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCAGGCATAATATTAGCCTTCAGGCTGGTTTCct                                                                                                           >  2:557320/1‑140 (MQ=255)                            cccGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTCCAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCAGGCATAATATTAGCCTTCAGGCTGGTTTCct                                                                                                           >  2:113839/1‑140 (MQ=255)                                  aTCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTCCAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCAGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGt                                                                                                     <  1:113839/140‑1 (MQ=255)                                    cGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTCCAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCAGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCg                                                                                                   <  1:95045/140‑1 (MQ=255)                                     gCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTCCAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCAGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGa                                                                                                  >  2:266024/1‑140 (MQ=255)                                       ccATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTCCGGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCAGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATa                                                                                                <  2:387579/140‑1 (MQ=255)                                       ccATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTCCAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCAGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATa                                                                                                <  1:266024/140‑1 (MQ=255)                                              cGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTCCAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCAGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATAcc                                                                                              >  1:403678/1‑135 (MQ=255)                                              cGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTCCAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCAGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATAcc                                                                                              <  2:403678/135‑1 (MQ=255)                                                     aTCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTCCAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCAGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATa                                                                                                <  2:542344/126‑1 (MQ=255)                                                     aTCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTCCAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCAGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATa                                                                                                >  1:542344/1‑126 (MQ=255)                                                     aTCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTCCAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCAGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATACCGTATTTAATaaa                                                                                  >  2:66177/1‑140 (MQ=255)                                                     aTCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTCCAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCAGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATACCGTATTTAATaaa                                                                                  >  2:598284/1‑140 (MQ=255)                                                          ttctctGACCGTCACGCCCTCGTCATTGACATACTCCAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCAGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATACCGTATTTAATAAACGAAc                                                                             <  1:64209/140‑1 (MQ=255)                                                               tGACCGTCACGCCCTCGTCATTGACATACTCCAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCAGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATACCGTATTTAATAAACGa                                                                               >  1:328435/1‑133 (MQ=255)                                                               tGACCGTCACGCCCTCGTCATTGACATACTCCAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCAGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATACCGTATTTAATAAACGa                                                                               <  2:328435/133‑1 (MQ=255)                                                                 aCCGTCACGCCCTCGTCATTGACATACTCCAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCAGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATACCGTATTTAATAAACGAACCTTTCtt                                                                      >  1:628507/1‑140 (MQ=255)                                                                 aCCGTCACGCCCTCGTCATTGACATACTCCAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCAGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATACCGTATTTAATAAACGAACCTTTCtt                                                                      >  2:657224/1‑140 (MQ=255)                                                                 aCCGTCACGCCCTCGTCATTGACATACTCCAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCAGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATACCGTATTTAATAAACGAACCTTTCtt                                                                      >  2:202720/1‑140 (MQ=255)                                                                      cACGCCCTCGTCATTGACATACTCCAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCAGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATACCGTATTTAATAAACGAACCTTTCTTACCGt                                                                 <  1:202720/140‑1 (MQ=255)                                                                                  aTTGACATACTCCAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCAGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATACCGTATTTAATAAACGAACCTTTCTTACCGTGAACGATAAAtt                                                     <  2:628507/140‑1 (MQ=255)                                                                                         tACTCCAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCAGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATACCGTATTTAATAAACGAACCTTTCTTACCGTGAACGATAAATTTCGGATa                                              >  1:19683/1‑140 (MQ=255)                                                                                             ccAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCAGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATACCGTATTTAATAAACGAACCTTTCTTACCGTGAACGATAAATTTCGGATAATCg                                          <  2:102232/140‑1 (MQ=255)                                                                                                gCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCAGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATACCGTATTTAATAAACGAACCTTTCTTACCGTGAACGATAAATTTCGGATAATCGAtt                                       >  1:290295/1‑140 (MQ=255)                                                                                                 cacaCCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCAGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATACCGTATTTAATAAACGAACCTTTCTTACCGTGAACGATAAATTTCGGATAATCGAttt                                      >  1:602176/1‑140 (MQ=255)                                                                                                        accgaATCATCCGCTGCGAATCCCGGTTCGCCAGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATACCGTATTTAATAAACGAACCTTTCTTACCGTGAACGATAAATTTCGGATAATCGATTTTCACCAg                               >  1:61222/1‑140 (MQ=255)                                                                                                           gaATCATCCGCTGCGAATCCCGGTTCGCCAGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATACCGTATTTAATAAACGAACCTTTCTTACCGTGAACGATAAATTTCGGATAATCGATTTTCACCAGATg                            <  1:557320/140‑1 (MQ=255)                                                                                                                         gAATCCCGGTTCGCCAGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATACCGTATTTAATAAACGAACCTTTCTTACCGTGAACGATAAATTTCGGATAATCGATTTTCACCAGATGGCTGGTTTTGACGa              <  1:66177/140‑1 (MQ=255)                                                                                                                          aaTCCCGGTTCGCCAGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATACCGTATTTAATAAACGAACCTTTCTTACCGTGAACGATAAATTTCGGATAATCGATTTTCACCAGATGGCTGGTTTTGACGAt             <  2:602176/140‑1 (MQ=255)                                                                                                                                    cGCCAGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATACCGTATTTAATAAACGAACCTTTCTTACCGTGAACGATAAATTTCGGATAATCGATTTTCACCAGATGGCTGGTTTTGACGATGGCTTTCAgg   >  2:122520/1‑140 (MQ=255)                                                                                                                                     gCCAGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATACCGTATTTAATAAACGAACCTTTCTTACCGTGAACGATAAATTTCGGATAATCGATTTTCACCAGATGGCTGGTTTTGACGATGGCTTTCAGGt  <  2:548751/140‑1 (MQ=255)                                                                                                                                     gCCAGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATACCGTATTTAATAAACGAACCTTTCTTACCGTGAACGATAAATTTCGGATAATCGATTTTCACCAGATGGCTGGTTTTGACGATGGCTTTCAGGt  <  1:657224/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         GGTTTGATACAACGCATCATAAACGCGCCCGTAATCGCCCATCTCCGGCTTCATCTCTTCTCTGACCGTCACGCCCTCGTCATTGACATACTCCAGCACACCGACCGAATCATCCGCTGCGAATCCCGGTTCGCCCGGCATAATATTAGCCTTCAGGCTGGTTTCCTGCTGGTCGATACCGTATTTAATAAACGAACCTTTCTTACCGTGAACGATAAATTTCGGATAATCGATTTTCACCAGATGGCTGGTTTTGACGATGGCTTTCAGGT  >  NC_000913/3579882‑3580153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑