Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,678,185	G→T	L56M (CTG→ATG)	yhjG ←	AsmA family protein YhjG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,678,185	0	G	T	100.0%	81.9 / NA	24	L56M (CTG→ATG)	yhjG	AsmA family protein YhjG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (11/13);  total (11/13)

TGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCGTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCAGATCGCCACGGATAGCGAACGGACGATTCAACTCCGCAGAGACTTTCTGGTTGATAGTCGGTTTGAGTCGATTCCAGTCAAAAGTTGCAATCAAAATGATCGCCACGACAATCAACAACAAGAAAGCCCCTGAAAT  >  NC_000913/3678051‑3678320                                                                                                                                       |                                                                                                                                        tGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCGTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCATATCGc                                                                                                                                    >  2:486921/1‑140 (MQ=255) tGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCGTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCATATCGc                                                                                                                                    >  1:94610/1‑140 (MQ=255)  ggCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCGTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCATATCGcc                                                                                                                                   <  2:144765/140‑1 (MQ=255)   gCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCGTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCATATCGCCa                                                                                                                                  >  1:536278/1‑140 (MQ=255)   gCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCGTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCATATCGCCa                                                                                                                                  >  2:105109/1‑140 (MQ=255)    cAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCGTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCATATCGCCAc                                                                                                                                 >  1:383180/1‑140 (MQ=255)     aaaTGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCGTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCATATCGCCACg                                                                                                                                >  2:291588/1‑140 (MQ=255)                cGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCGTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCATATCGCCACGGATAGCGAACg                                                                                                                     <  1:277070/140‑1 (MQ=255)                       tCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCGTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCATATCGCCACGGATAGCGAACGGACGAtt                                                                                                              <  1:2719/140‑1 (MQ=255)                               atCCGGTGGATTGCCAAGAATGATGTCTTCCGCGTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCATATCGCCACGGATAGCGAACGGACGATTCAACTCCg                                                                                                      <  1:291588/140‑1 (MQ=255)                                tCCGGTGGATTGCCAAGAATGATGTCTTCCGCGTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCATATCGCCACGGATAGCGAACGGACGATTCAACTCCGc                                                                                                     <  2:94610/140‑1 (MQ=255)                                     tGGATTGCCAAGAATGATGTCTTCCGCGTGTACATGGGGCCACGGCTCCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACGCCACGCCCATATGGCCACGGATGGCGAACGGACGATTCAACTCCGCAgaga                                                                                                <  2:145666/140‑1 (MQ=255)                                          tGCCAAGAATGATGTCTTCCGCGTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCATATCGCCACGGATAGCGAACGGACGATTCAACTCCGCAGAGACTTTc                                                                                           >  1:740775/1‑140 (MQ=255)                                               aGAATGATGTCTTCCGCGTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCATATCGCCACGGATAGCGAACGGACGATTCAACTCCGCAGAGACTTTCTGGtt                                                                                      <  2:154410/140‑1 (MQ=255)                                                          ttCCGCGTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCATATCGCCACGGATAGCGAACGGACGATTCAACTCCGCAGAGACTTTCTGGTTGATAGTCGGtt                                                                           <  2:561590/140‑1 (MQ=255)                                                           tCCGCGTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCATATCGCCACGGATAGCGAACGGACGATTCAACTCCGCAGAGACTTTCTGGTTGATAGTCGGttt                                                                          >  1:595411/1‑140 (MQ=255)                                                                                             gccagccaGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCATATCGCCACGGATAGCGAACGGACGATTCAACTCCGCAGAGACTTTCTGGTTGATAGTCGGTTTGAGTCGATTCCAGTCAAAAGTTGCAATCAAAATg                                        <  2:100370/140‑1 (MQ=255)                                                                                             gccagccaGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCATATCGCCACGGATAGCGAACGGACGATTCAACTCCGCAGAGACTTTCTGGTTGATAGTCGGTTTGAGTCGATTCCAGTCAAAAGTTGCAATCAAAATg                                        <  1:612493/140‑1 (MQ=255)                                                                                             gccagccaGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCATATCGCCACGGATAGCGAACGGACGATTCAACTCCGCAGAGACTTTCTGGTTGATAGTCGGTTTGAGTCGATTCCAGTCAAAAGTTGCAATCAAAATg                                        <  2:536278/140‑1 (MQ=255)                                                                                                 gccagTTTCTTGTTTTTGCCGCTCCCACACCACGCCCATATCGCCACGGATAGCGAACGGACGATTCAACTCCGCAGAGACTTTCTGGTTGATAGTCGGTTTGAGTCGATTCCAGTCAAAAGTTGCAATCAAAATGATCg                                    >  2:605514/1‑140 (MQ=255)                                                                                                       ttCTTGTTTTTGCCGCTCCCACACCACGCCCATATCGCCACGGATAGCGAACGGACGATTCAACTCCGCAGAGACTTTCTGGTTGATAGTCGGTTTGAGTCGATTCCAGTCAAAAGTTGCAATCAAAATGATCGCCACGa                              <  1:105109/140‑1 (MQ=255)                                                                                                                  gCCGCTCCCACACCACGCCCATATCGCCACGGATAGCGAACGGACGATTCAACTCCGCAGAGACTTTCTGGTTGATAGTCGGTTTGAGTCGATTCCAGTCAAAAGTTGCAATCAAAATGATCGCCACGACAATcaacaac                   <  1:605514/140‑1 (MQ=255)                                                                                                                      cTCCCACACCACGCCCATATCGCCACGGATAGCGAACGGACGATTCAACTCCGCAGAGACTTTCTGGTTGATAGTCGGTTTGAGTCGATTCCAGTCAAAAGTTGCAATCAAAATGATCGCCACGACAATCAACAACAAGa               <  2:595411/140‑1 (MQ=255)                                                                                                                                 cGCCCATATCGCCACGGATAGCGAACGGACGATTCAACTCCGCAGAGACTTTCTGGTTGATAGTCGGTTTGAGTCGATTCCAGTCAAAAGTTGCAATCAAAATGATCGCCACGACAATCAACAACAAGAAAGCCCCTGaa    >  2:565044/1‑140 (MQ=255)                                                                                                                                   cccATATCGCCACGGATAGCGAACGGACGATTCAACTCCGCAGAGACTTTCTGGTTGATAGTCGGTTTGAGTCGATTCCAGTCAAAAGTTGCAATCAAAATGATCGCCACGACAATCAACAACAAGAAAGCCCCTGAAAt  >  1:316164/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        TGGCAAATGCACCATCGTGACTTCGGGAATATCCGGTGGATTGCCAAGAATGATGTCTTCCGCGTGTACATGGGGCCACGGCACCCAGCTGCGCCAGCCAGTTTCTTGTTTTTGCCGCTCCCACACCACGCCCAGATCGCCACGGATAGCGAACGGACGATTCAACTCCGCAGAGACTTTCTGGTTGATAGTCGGTTTGAGTCGATTCCAGTCAAAAGTTGCAATCAAAATGATCGCCACGACAATCAACAACAAGAAAGCCCCTGAAAT  >  NC_000913/3678051‑3678320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑