Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,772,095	C→A	intergenic (‑351/‑186)	yibI ← / → mtlA	DUF3302 domain‑containing protein YibI/mannitol‑specific PTS enzyme IICBA component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,772,095	0	C	A	100.0%	186.6 / NA	53	intergenic (‑351/‑186)	yibI/mtlA	DUF3302 domain‑containing protein YibI/mannitol‑specific PTS enzyme IICBA component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (18/35);  total (18/35)

GTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTCAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGGCCGTCGACTGGACAGTTAACCGATTCAGTGCCAGATTTCGCAGTATCTACAAGGTCCGGCTACCTCTGCCGCCACATTA  >  NC_000913/3771962‑3772223                                                                                                                                      |                                                                                                                                 gtgATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTAc                                                                                                                            <  1:729805/140‑1 (MQ=255) gtgATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTAc                                                                                                                            <  1:156040/140‑1 (MQ=255) gtgATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTAc                                                                                                                            <  1:251889/140‑1 (MQ=255)  tgATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTAcc                                                                                                                           >  2:476477/1‑140 (MQ=255)  tgATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTAc                                                                                                                            <  2:508839/139‑1 (MQ=255)  tgATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTAc                                                                                                                            >  1:508839/1‑139 (MQ=255)    aTTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATCCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTACctc                                                                                                                         >  1:369077/1‑140 (MQ=255)     ttCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACGCAAGCGTTTTGTGATGAACGTCACGTAAATTACctct                                                                                                                        >  1:359961/1‑140 (MQ=255)      tCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTACctctc                                                                                                                       <  1:476477/140‑1 (MQ=255)       cAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTACctctct                                                                                                                      <  1:677853/140‑1 (MQ=255)       cAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTACctctct                                                                                                                      <  2:528909/140‑1 (MQ=255)        aGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTACCTCTCTa                                                                                                                     <  2:378290/140‑1 (MQ=255)          aTCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTAcc                                                                                                                           >  2:606383/1‑132 (MQ=255)          aTCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTAcc                                                                                                                           <  1:606383/132‑1 (MQ=255)          aTCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTACCTCTCTAcc                                                                                                                   >  1:272412/1‑140 (MQ=255)            cacaAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTACCTCTCTAcccc                                                                                                                 <  2:164366/140‑1 (MQ=255)            cacaAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTACCTCTCTAcccc                                                                                                                 <  1:724698/140‑1 (MQ=255)            cacaAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTACCTCTCTAcccc                                                                                                                 <  1:148096/140‑1 (MQ=255)            cacaAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTACCTCTCTAcccc                                                                                                                 <  1:39069/140‑1 (MQ=255)            cacaAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTACCTCTCTAcccc                                                                                                                 <  2:62897/140‑1 (MQ=255)            cacaAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTACCTCTCTAcccc                                                                                                                 <  2:723498/140‑1 (MQ=255)                aaGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTACCTCTCTACCCCCtat                                                                                                             <  1:202250/140‑1 (MQ=255)                  gATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACGCAAGCGTTTTGTGATGAACGTCACGTAAATTACCTCTCTACCCCCTatat                                                                                                           <  2:359961/140‑1 (MQ=255)                    ttCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTACCTCTCTACCCCCTATAttt                                                                                                         >  2:674019/1‑140 (MQ=255)                      caacaaACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTACCTCTCTAcccc                                                                                                                 <  1:127786/130‑1 (MQ=255)                      caacaaACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTACCTCTCTAcccc                                                                                                                 >  2:127786/1‑130 (MQ=255)                      caacaaACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTACCTCTCTACCCCCTATATTTAt                                                                                                       <  2:247983/140‑1 (MQ=255)                      caacaaACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTACCTCTCTACCCCCTATATTTAt                                                                                                       <  2:118527/140‑1 (MQ=255)                             ccATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCA‑TTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTACCTCTCTACCCCCTATATTTATGtgattga                                                                                               <  1:656681/140‑1 (MQ=255)                              cATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTACCTCTCTACCCCCTATATTTATGtgattga                                                                                               <  2:635225/140‑1 (MQ=255)                                tCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTACCTCTCTACCCCCTATATTTATGTGATTGata                                                                                             <  2:220200/140‑1 (MQ=255)                                        aaaaTGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACaa                                                                                     >  1:715291/1‑140 (MQ=255)                                              tgACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGGcc                                                                               >  1:339589/1‑140 (MQ=255)                                              tgACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGGcc                                                                               <  2:401614/140‑1 (MQ=255)                                               gACACTACTCACATTTAAATGCCATTTTTATCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTACCTCTCTACCCCCTATATTTATGTGATTGATAT‑‑CACAAAAGGCCGTc                                                                            >  1:330690/1‑140 (MQ=255)                                                       tCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGGCCGTCGACTgg                                                                      <  2:704446/140‑1 (MQ=255)                                                                aaTGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACTCAAAAGGCCGTCGACTGGACAGTTAAc                                                             >  1:188258/1‑140 (MQ=255)                                                                aaTGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGGCCGTCGACTg                                                                       <  1:717293/130‑1 (MQ=255)                                                                aaTGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGGCCGTCGACTg                                                                       >  2:717293/1‑130 (MQ=255)                                                                     cATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGGCCGTCGACTGGACAGTTAACCGAtt                                                        >  1:370697/1‑140 (MQ=255)                                                                                    tCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGGCCGTCGACTGGACAGTTAACCGATTCAGTGCCAGATTTCg                                         <  1:674019/140‑1 (MQ=255)                                                                                    tCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGGCCGTCGACTGGACAGTTAACCGATTCAGTGCCAGATTTCg                                         <  1:313950/140‑1 (MQ=255)                                                                                    tCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGGCCGTCGACTGGACAGTTAACCGATTCAGTGCCAGATTTCg                                         <  2:148564/140‑1 (MQ=255)                                                                                    tCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGGCCGTCGACTGGACAGTTAACCGATTCAGTGCCAGATTTCg                                         <  2:91723/140‑1 (MQ=255)                                                                                       ccgccTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTACCTCTCTACCCCCTATATTTATGTGATTGATAT‑‑CACAAAAGGCCGTCGACTGGACAGTTAACCGATTCAGTGCCAGATTTCGCAGTa                                    <  2:330690/140‑1 (MQ=255)                                                                                                  cTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTAAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGGCCGTCGACTGGACAGTTAACCGATTCAGTGCCAGATTTCGCAGTATCTACAAgg                           <  2:369077/140‑1 (MQ=255)                                                                                                   ttttttACACAAGCGTTTTGTGATGAACGTCACGTAAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGGCCGTCGACTGGACAGTTAACCGATTCAGTGCCAGATTTCGCAGTATCTACAAGGt                          <  2:339589/140‑1 (MQ=255)                                                                                                       ttACACAAGCGTTTTGTGATGAACGTCACGTAAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGGCCGTCGACTGGACAGTTAACCGATTCAGTGCCAGATTTCGCAGTATCTACAAGGTCCgg                      >  2:325483/1‑140 (MQ=255)                                                                                                               gCGTTTTGTGATGAACGTCACGTAAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGGCCGTCGACTGGACAGTTAACCGATTCAGTGCCAGATTTCGCAGTATCTACAAGGTCCGGCTACctct              >  2:680260/1‑140 (MQ=255)                                                                                                                         atgaACGTCACGTAAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGGCCGTCGACTGGACAGTTAACCGATTCAGTGCCAGATTTCGCAGTATCTACAAGGTCCGGCTACCTCTgccgc         >  2:182547/1‑135 (MQ=255)                                                                                                                         atgaACGTCACGTAAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGGCCGTCGACTGGACAGTTAACCGATTCAGTGCCAGATTTCGCAGTATCTACAAGGTCCGGCTACCTCTgccgc         <  1:182547/135‑1 (MQ=255)                                                                                                                           gaACGTCACGTAAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGGCCGTCGACTGGACAGTTAACCGATTCAGTGCCAGATTTCGCAGTATCTACAAGGTCCGGCTACCTCTGCCGCCACATTa  <  1:525122/140‑1 (MQ=255)                                                                                                                           gaACGTCACGTAAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGGCCGTCGACTGGACAGTTAACCGATTCAGTGCCAGATTTCGCAGTATCTACAAGGTCCGGCTACCTCTGCCGCCACATTa  >  2:219383/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                 GTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTCAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGGCCGTCGACTGGACAGTTAACCGATTCAGTGCCAGATTTCGCAGTATCTACAAGGTCCGGCTACCTCTGCCGCCACATTA  >  NC_000913/3771962‑3772223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑