Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,186,763	C→A	L472M (CTG→ATG)	rpoC →	RNA polymerase subunit beta'

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,186,763	0	C	A	100.0%	187.3 / NA	53	L472M (CTG→ATG)	rpoC	RNA polymerase subunit beta'
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (26/27);  total (26/27)

ACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGATGGCTGTTCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTGACT  >  NC_000913/4186631‑4186898                                                                                                                                     |                                                                                                                                        aCTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCt                                                                                                                                  >  1:241463/1‑140 (MQ=255)            tCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGc                                                                                                                       >  2:711931/1‑140 (MQ=255)            tCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGc                                                                                                                       >  1:195507/1‑140 (MQ=255)            tCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGc                                                                                                                       >  1:248073/1‑140 (MQ=255)            tCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGATGGCTGTTCACGAACCGATGACGCTGGAAGCCCAGc                                                                                                                       >  2:572034/1‑140 (MQ=255)                      cAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGcgcg                                                                                                             <  2:12189/140‑1 (MQ=255)                       aGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGt                                                                                                            >  1:685074/1‑140 (MQ=255)                              tGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCtga                                                                                                     >  2:456752/1‑140 (MQ=255)                              tGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCtga                                                                                                     <  1:617/140‑1 (MQ=255)                              tGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCtga                                                                                                     >  2:597832/1‑140 (MQ=255)                               gAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCtgat                                                                                                    <  2:533556/140‑1 (MQ=255)                                    ggTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGt                                                                                               <  2:38301/140‑1 (MQ=255)                                    ggTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGt                                                                                               <  1:567103/140‑1 (MQ=255)                                     gTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTc                                                                                              >  1:422325/1‑140 (MQ=255)                                        cTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTAc                                                                                           <  1:11768/140‑1 (MQ=255)                                             cGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACcaaca                                                                                      >  1:610674/1‑140 (MQ=255)                                                           tCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCcg                                                                        >  2:560563/1‑140 (MQ=255)                                                           tCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCcg                                                                        >  1:83543/1‑140 (MQ=255)                                                                cTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCcggcgaa                                                                   >  2:578887/1‑140 (MQ=255)                                                                 tGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCcggcgaac                                                                  >  1:95027/1‑140 (MQ=255)                                                                 tGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCcggcgaac                                                                  >  1:272671/1‑140 (MQ=255)                                                                     cccGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCcggcgaacggcg                                                              >  2:251811/1‑140 (MQ=255)                                                                       cGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCcggcgaacggcgaa                                                            <  1:251811/140‑1 (MQ=255)                                                                          tGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCa                                                         <  1:456752/140‑1 (MQ=255)                                                                              ttGTGCGGCATATAACGCCGACTTCGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAatca                                                     <  1:597832/140‑1 (MQ=255)                                                                                gtgCGGCATATAACGCCGACTTCGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCcggcgaacggcg                                                              <  1:487909/129‑1 (MQ=255)                                                                                gtgCGGCATATAACGCCGACTTCGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCcggcgaacggcg                                                              >  2:487909/1‑129 (MQ=255)                                                                                    ggCATATAACGCCGACTTCGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTc                                               >  2:634731/1‑140 (MQ=255)                                                                                    ggCATATAACGCCGACTTCGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTc                                               <  1:196181/140‑1 (MQ=255)                                                                                           aaCGCCGACTTCGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCa                                        <  2:19693/140‑1 (MQ=255)                                                                                                cGACTTCGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTc                                               <  1:353607/128‑1 (MQ=255)                                                                                                cGACTTCGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTc                                               >  2:353607/1‑128 (MQ=255)                                                                                                cGACTTCGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACg                                   >  1:268966/1‑140 (MQ=255)                                                                                                cGACTTCGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACg                                   <  1:682905/140‑1 (MQ=255)                                                                                                cGACTTCGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACg                                   >  2:682905/1‑140 (MQ=255)                                                                                                cGACTTCGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACg                                   >  1:410384/1‑140 (MQ=255)                                                                                                cGACTTCGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACg                                   <  2:264353/140‑1 (MQ=255)                                                                                                cGACTTCGATGGCGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACg                                   >  2:389123/1‑140 (MQ=255)                                                                                                 gACTTCGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGt                                  <  1:564681/140‑1 (MQ=255)                                                                                                  aCTTCGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGtt                                                <  1:127319/125‑1 (MQ=255)                                                                                                  aCTTCGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGtt                                                >  2:127319/1‑125 (MQ=255)                                                                                                      cGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTAc                             <  2:410384/140‑1 (MQ=255)                                                                                                      cGATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTAc                             <  2:128306/140‑1 (MQ=255)                                                                                                      cGATGGTGACCAGATGGCTGTTCACGAACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTAc                             <  1:572034/140‑1 (MQ=255)                                                                                                       gATGGTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACt                            <  1:389123/140‑1 (MQ=255)                                                                                                           gggACCAGATGGCTGTTCTCGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGt                        <  2:77609/138‑1 (MQ=255)                                                                                                           gTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGt                        <  2:195507/140‑1 (MQ=255)                                                                                                           gTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGt                        <  1:501859/140‑1 (MQ=255)                                                                                                           gTGACCAGATGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGt                        <  2:83543/140‑1 (MQ=255)                                                                                                                   aTGGCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTTtacta                >  2:74301/1‑140 (MQ=255)                                                                                                                     ggCTGTTCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACa              <  2:248073/140‑1 (MQ=255)                                                                                                                          ttCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACa              <  2:193631/135‑1 (MQ=255)                                                                                                                          ttCACGTACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACa              >  1:193631/1‑135 (MQ=255)                                                                                                                                tACCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTGAc   <  2:272671/140‑1 (MQ=255)                                                                                                                                 aCCGATGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTTTACTACATGACCCGTGACt  <  1:74301/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                        ACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGATGGCTGTTCACGTACCGCTGACGCTGGAAGCCCAGCTGGAAGCGCGTGCGCTGATGATGTCTACCAACAACATCCTGTCCCCGGCGAACGGCGAACCAATCATCGTTCCGTCTCAGGACGTTGTACTGGGTCTGTACTACATGACCCGTGACT  >  NC_000913/4186631‑4186898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑