Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	801,366	C→A	A203D (GCT→GAT)	ybhH →	putative isomerase YbhH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	801,366	0	C	A	100.0%	138.1 / NA	41	A203D (GCT→GAT)	ybhH	putative isomerase YbhH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (18/23);  total (18/23)

ACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCAGATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAGCATTATTAGCCCGCATTGAATCTATCCGTCTACAAGCGGGTAAAGCAATGGGCTTAGGTGATGTCA  >  NC_000913/801229‑801488                                                                                                                                          |                                                                                                                           aCCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCAGATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGATg                                                                                                                          <  1:547619/140‑1 (MQ=255)     gTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCAGATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGATGAata                                                                                                                      >  2:252139/1‑140 (MQ=255)              aCTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCAGATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGATGAATATCTGGGaaa                                                                                                             >  2:257299/1‑140 (MQ=255)                tttCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCAGATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGATGAATATCTGGGaaaaa                                                                                                           <  1:218816/140‑1 (MQ=255)                tttCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCAGATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGATGAATATCTGGGaaaaa                                                                                                           <  2:257893/140‑1 (MQ=255)                tttCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCAGATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGATGAATATCTGGGaaaaa                                                                                                           <  2:567508/140‑1 (MQ=255)                  tCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGATGAATATCTGGGAAAAACa                                                                                                         >  2:714487/1‑140 (MQ=255)                        aTGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCAGATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGATGAATATCTGGGAAAAACAGGTTAt                                                                                                   >  2:409279/1‑140 (MQ=255)                          gCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCAGATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGATGAATATCTGGGAAAAACAGGTTATGa                                                                                                 >  1:306515/1‑140 (MQ=255)                                        aaCCGGAAAAGTTTTCCCGACTGATAATCAGATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGATGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAAc                                                                                   >  2:700728/1‑140 (MQ=255)                                             gAAAAGTTTTCCCGACTGATAATCAGATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGATGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGAt                                                                              >  2:747422/1‑140 (MQ=255)                                                   ttttCCCGACTGATAATCAGATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGATGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGAc                                                                        <  1:747422/140‑1 (MQ=255)                                                   ttttCCCGACTGATAATCAGATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGATGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGAc                                                                        <  2:138184/140‑1 (MQ=255)                                                     ttCCCGACTGATAATCAGATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGATGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACaa                                                                      >  2:427511/1‑140 (MQ=255)                                                     ttCCCGACTGATAATCAGATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGATGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACaa                                                                      <  1:252139/140‑1 (MQ=255)                                                       cccGACTGATAATCAGATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGATGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAg                                                                    <  1:700728/140‑1 (MQ=255)                                                            cTGATAATCAGATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGAAATGGCGATGCCAGTCGTCATTATTCCGGATGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAGCatta                                                               <  1:121172/140‑1 (MQ=255)                                                                 aaTCAGATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGATGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAGCATTATTAGc                                                          <  1:409279/140‑1 (MQ=255)                                                                         tgattATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTTCGGATGAATATCTGGGAAAATCAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAGCATTATTAGCCCGCATTg                                                  >  1:263300/1‑140 (MQ=255)                                                                         tgattATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGATGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAGCATTATTAGCCCGCATTg                                                  >  2:129899/1‑140 (MQ=255)                                                                           attattTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGATGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAGCATTATTAGCCCGCATTGaa                                                >  1:51545/1‑140 (MQ=255)                                                                           attattTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGATGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAGCATTATTAGCCCGCATTGaa                                                >  2:560292/1‑140 (MQ=255)                                                                                tttGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGATGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAGCATTATTAGCCCGCATTGAATCTAt                                           >  2:93466/1‑140 (MQ=255)                                                                                                 gACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGATGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAGCATTATTAGCCCGCATTGAATCTATCCGTCTACAAGCGGGTa                          <  1:129899/140‑1 (MQ=255)                                                                                                 gACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGATGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAGCATTATTAGCCCGCATTGAATCTATCCGTCTACAAGCGGGTa                          <  1:93466/140‑1 (MQ=255)                                                                                                  aCCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGATGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAGCATTATTAGCCCGCATTGAATCTATCCGTCTACAAGCGGGTaa                         >  2:715211/1‑140 (MQ=255)                                                                                                    cTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGATGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAGCATTATTAGCCCGCATTGAATCTATCCGTCTACAAGCGGGTAAAg                       >  2:389942/1‑140 (MQ=255)                                                                                                      gTATCGATATGGCGATGCCAGTCGTCATTATTCCGGATGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAGCATTATTAGCCCGCATTGAATCTATCCGTCTACAAGCGGGTAAAGCa                     <  2:51545/140‑1 (MQ=255)                                                                                                      gTATCGATATGGCGATGCCAGTCGTCATTATTCCGGATGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAGCATTATTAGCCCGCATTGAATCTATCCGTCTACAAGCGGGTAAAGCa                     <  1:18512/140‑1 (MQ=255)                                                                                                       tATCGATATGGCGATGCCAGTCGTCATTATTCCGGATGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAGCATTATTAGCCCGCATTGAATCTATCCGTCTACAAGCGGGTAAAGCaa                    <  1:715211/140‑1 (MQ=255)                                                                                                            atatGGCGATGCCAGTCGTCATTATTCCGGATGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAGCATTATTAGCCCGCATTGAATCTATCCGTCTACAAGCGGGTAAAGCAATGGGc               >  1:573019/1‑140 (MQ=255)                                                                                                               tGGCGATGCCAGTCGTCATTATTCCGGATGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAGCATTATTAGCCCGCATTGAATCTATCCGTCTACAAGCGGGTAAAGCAATGGGCTTa            <  1:714487/140‑1 (MQ=255)                                                                                                                 gCGATGCCAGTCGTCATTATTCCGGATGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAGCATTATTAGCCCGCATTGAATCTATCCGTCTACAAGCGGGTAAAGCAATGGGCTTAgg          >  1:698651/1‑140 (MQ=255)                                                                                                                  cGATGCCAGTCGTCATTATTCCGGATGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAGCATTATTAGCCCGCATTGAATCTATCCGTCTACAAGCGGGTAAAGCAATGGGCTTAGGt         <  2:233685/140‑1 (MQ=255)                                                                                                                  cGATGCCAGTCGTCATTATTCCGGATGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAGCATTATTAGCCCGCATTGAATCTATCCGTCTACAAGCGGGTAAAGCAATGGGCTTAGGt         <  2:306515/140‑1 (MQ=255)                                                                                                                  cGATGCCAGTCGTCATTATTCCGGATGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAGCATTATTAGCCCGCATTGAATCTATCCGTCTACAAGCGGGTAAAGCAATGGGCTTAGGt         <  2:573019/140‑1 (MQ=255)                                                                                                                  cGATGACAGTCGTCATTATTCCGGATGAATATCTGAGAAAAACAGGTTATGAATTACCGACGGAACTGGATGCCGACAAAGCATTATTAGCCCGCATTGAATCTATCCGTCTACAAGCGGGTAAAGCAATGGGCTTAGGt         <  2:263300/140‑1 (MQ=255)                                                                                                                       ccAGTCGTCATTATTCCGGATGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAGCATTATTAGCCCGCATTGAATCTATCCGTCTACAAGCGGGTAAAGCAATGGGCTTAGGTGATGt    <  2:698651/140‑1 (MQ=255)                                                                                                                       ccAGTCGTCATTATTCCGGATGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAGCATTATTAGCCCGCATTGAATCTATCCGTCTACAAGCGGGTAAAGCAATGGGCTTAGGTGATGt    <  2:592223/140‑1 (MQ=255)                                                                                                                         aGTCGTCATTATTCCGGATGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAGCATTATTAGCCCGCATTGAATCTATCCGTCTACAAGCGGGTAAAGCAATGGGCTTAGGTGATGTCa  <  1:560292/140‑1 (MQ=255)                                                                                                                            cgtcATTATTCCGGATGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAGCATTATTAGCCCGCATTGAATCTATCCGTCTACAAGCGGGTAAAGCAATGGGCTTAg           <  1:568179/128‑1 (MQ=255)                                                                                                                            cgtcATTATTCCGGATGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAGCATTATTAGCCCGCATTGAATCTATCCGTCTACAAGCGGGTAAAGCAATGGGCTTAg           >  2:568179/1‑128 (MQ=255)                                                                                                                                          |                                                                                                                           ACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCAGATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAGCATTATTAGCCCGCATTGAATCTATCCGTCTACAAGCGGGTAAAGCAATGGGCTTAGGTGATGTCA  >  NC_000913/801229‑801488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑