Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	812,765	G→T	A166S (GCC→TCC)	bioD →	dethiobiotin synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	812,765	0	G	T	100.0%	135.8 / NA	39	A166S (GCC→TCC)	bioD	dethiobiotin synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (23/16);  total (23/16)

TGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAG  >  NC_000913/812630‑812894                                                                                                                                        |                                                                                                                                  tGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACTCCgg                                                                                                                               >  2:55703/1‑140 (MQ=255)             cTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACTCCGGACTGACTCTGGc                                                                                                                   >  2:74009/1‑140 (MQ=255)                   gACACTTTCACTTTTGCAGATTGGGTAACACAGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACTCCGGACTGACTCTGGCGGGTTg                                                                                                             <  1:427590/140‑1 (MQ=255)                          tcactttTGCAGATTGGGTAACACAGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACTCCGGACTGACTCTGGCGGGTTGGGTGGCg                                                                                                      >  1:52202/1‑140 (MQ=255)                                ttGCAGATTGGGTAACACAGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACTCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGAt                                                                                                >  2:627579/1‑140 (MQ=255)                                      aTTGGGTAACACAGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACTCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACg                                                                                          >  2:579718/1‑140 (MQ=255)                                          ggTAACACAGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACTCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTc                                                                                      <  2:610781/140‑1 (MQ=255)                                          ggTAACACAGGAACAACTGCCGGAGATACTGGTCGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACTCCGAACTGAATCTGGCGGGATGGGTGGCGAACGATGTTACGCCTc                                                                                      >  2:277278/1‑140 (MQ=255)                                                     aacaacTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACTCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGt                                                                           >  2:568427/1‑140 (MQ=255)                                                      acaacTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACTCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTc                                                                          <  1:756692/140‑1 (MQ=255)                                                        aacTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACTCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCAc                                                                        <  1:7282/140‑1 (MQ=255)                                                         acTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACTCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACg                                                                       <  1:55703/140‑1 (MQ=255)                                                         acTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACTCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACg                                                                       <  1:567119/140‑1 (MQ=255)                                                          cTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACTCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGc                                                                      >  1:137140/1‑140 (MQ=255)                                                          cTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACTCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGc                                                                      >  1:354789/1‑140 (MQ=255)                                                           tGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACTCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCt                                                                     <  1:627579/140‑1 (MQ=255)                                                             ccGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACTCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGa                                                                   >  2:44747/1‑140 (MQ=255)                                                             ccGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACTCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGa                                                                   <  2:354789/140‑1 (MQ=255)                                                               ggTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACTCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAat                                                                 >  2:240812/1‑140 (MQ=255)                                                                 tGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACTCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAatat                                                               <  2:309128/140‑1 (MQ=255)                                                                        ggTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACTCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCa                                                        <  2:496464/140‑1 (MQ=255)                                                                            gTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACTCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCt                                                    <  2:137140/140‑1 (MQ=255)                                                                                 tgtgAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACTCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCAccc                                               >  1:599481/1‑140 (MQ=255)                                                                                 tgtgAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACTCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCAccc                                               >  2:704066/1‑140 (MQ=255)                                                                                   tgAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACTCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGc                                             >  1:72/1‑140 (MQ=255)                                                                                   tgAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACTCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGc                                             >  2:579727/1‑140 (MQ=255)                                                                                           ggCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACTCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTcc                                     >  1:521537/1‑140 (MQ=255)                                                                                           ggCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACTCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTcc                                     >  1:32214/1‑140 (MQ=255)                                                                                                tATTAATCACGCGATGTTGACTGCACAGGTAATACAACACTCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCgcgc                                <  1:240812/140‑1 (MQ=255)                                                                                                  ttAATCACGCGATGTTGACTGCACAGGTAATACAACACTCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGcgccg                              <  2:52202/140‑1 (MQ=255)                                                                                                          gcgATGTTGACTGCACAGGTAATACAACACTCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTggg                      >  1:16272/1‑140 (MQ=255)                                                                                                              tGTTGACTGCACAGGTAATACAACACTCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGgagag                  <  1:277278/140‑1 (MQ=255)                                                                                                               gTTGACTGCACAGGTAATACAACACTCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGgagaga                 >  1:43786/1‑140 (MQ=255)                                                                                                               gTTGACTGCACAGGTAATACAACACTCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGgagaga                 >  2:626307/1‑140 (MQ=255)                                                                                                                      gCACAGGTAATACAACACTCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTg          >  1:125035/1‑140 (MQ=255)                                                                                                                        acaGGTAATACAACACTCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGc        >  2:182963/1‑140 (MQ=255)                                                                                                                          aGGTAATACAACACTCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTgg                       >  2:438298/1‑123 (MQ=255)                                                                                                                          aGGTAATACAACACTCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTgg                       <  1:438298/123‑1 (MQ=255)                                                                                                                            gTAATACAACACTCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGc    >  2:406304/1‑140 (MQ=255)                                                                                                                              aaTACAACACTCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAg  <  1:579718/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                  TGGTTTACGCCGCTTTCTGACACTTTCACTTTTGCAGATTGGGTAACACAGGAACAACTGCCGGTGATACTGGTAGTTGGTGTGAAACTCGGCTGTATTAATCACGCGATGTTGACTGCACAGGTAATACAACACGCCGGACTGACTCTGGCGGGTTGGGTGGCGAACGATGTTACGCCTCCGGGAAAACGTCACGCTGAATATATGACCACGCTCACCCGCATGATTCCCGCGCCGCTGCTGGGAGAGATCCCCTGGCTTGCAG  >  NC_000913/812630‑812894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑