Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,069,376	C→A	G155V (GGC→GTC)	rutG ←	pyrimidine:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,069,376	0	C	A	100.0%	155.9 / NA	45	G155V (GGC→GTC)	rutG	pyrimidine:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (21/24);  total (21/24)

GAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGCCAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAGTCGTTCAATCCAGCGCGTACCGATTTTCATCACCACCAGGCCAATGACGGTATAAACCAGCCCACAGGCGATAATCCCGCCCAG  >  NC_000913/1069240‑1069503                                                                                                                                         |                                                                                                                                gagaATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGACaa                                                                                                                              >  2:726586/1‑140 (MQ=255) gagaATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGACaa                                                                                                                              <  1:107702/140‑1 (MQ=255)     aTCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGACAATCGc                                                                                                                          >  2:180958/1‑140 (MQ=255)           aaCCGCTGGATTATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGACAATCGCCATcac                                                                                                                    <  2:321080/140‑1 (MQ=255)                cTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGACAATCGCCATCACCACCg                                                                                                               <  1:288391/140‑1 (MQ=255)                        tGCCACGGGTAAACACGGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGACAATCGCCAt                                                                                                                       >  2:479814/1‑124 (MQ=255)                        tGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGACAATCGCCAt                                                                                                                       <  1:479814/124‑1 (MQ=255)                        tGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGACAATCGCCATCACCACCGCACCCGTc                                                                                                       >  1:571805/1‑140 (MQ=255)                              gggTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGACAATCGCCATCACCACCGCa                                                                                                             <  1:530984/128‑1 (MQ=255)                              gggTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGACAATCGCCATCACCACCGCa                                                                                                             >  2:530984/1‑128 (MQ=255)                               ggTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGACAATCGCCATCACCACCGCACCCGTCACCACcg                                                                                                >  2:7710/1‑140 (MQ=255)                                         gCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGACAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAg                                                                                      <  1:34369/140‑1 (MQ=255)                                         gCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGACAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAg                                                                                      <  1:726586/140‑1 (MQ=255)                                          ccaccaGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGACAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAGt                                                                                     >  1:224604/1‑140 (MQ=255)                                          ccaccaGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGACAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAGt                                                                                     >  1:674446/1‑140 (MQ=255)                                              caGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGACAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAGTCGtt                                                                                 <  1:281309/140‑1 (MQ=255)                                                gCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGACAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAGTCGTTCa                                                                               >  1:325677/1‑140 (MQ=255)                                                           aGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGACAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAGTCGTTCAATCCAGCGCGt                                                                    >  1:18342/1‑140 (MQ=255)                                                            gCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGACAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAGTCGTTCAATCCAGCGCGTa                                                                   >  2:219787/1‑140 (MQ=255)                                                            gCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGACAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAGTCGTTCAATCCAGCGCGTa                                                                   >  2:716502/1‑140 (MQ=255)                                                                cGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGACAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAGTCGTTCAATCCAGCGCGTACCGa                                                               >  2:19814/1‑140 (MQ=255)                                                                cGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGACAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAGTCGTTCAATCCAGCGCGTACCGa                                                               >  2:165115/1‑140 (MQ=255)                                                                  tCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGACAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAGTCGTTCAATCCAGCGCGTACCGAtt                                                             >  1:662444/1‑140 (MQ=255)                                                                   cATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGACAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAGTCGTTCAATCCAACGCGTACCGAttt                                                            <  1:184803/140‑1 (MQ=255)                                                                       aCCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGACAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAGTCGTTCAATCCAGCGCGTACCGATTTtcat                                                        >  1:695723/1‑140 (MQ=255)                                                                        ccGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGACAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAGTCGTTCAATCCAGCGCGTACCGATTTtcatc                                                       <  2:325677/140‑1 (MQ=255)                                                                             aTCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGACAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAGTCGTTCAATCCAGCGCGTACCGATTTTCATcaccac                                                  <  2:224604/140‑1 (MQ=255)                                                                                      tCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGACAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAGTCGTTCAATCCAGCGCGTACCGATTTTCATCACCACCAGGCCAAt                                         >  1:144056/1‑140 (MQ=255)                                                                                       cAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGACAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAGTCGTTCAATCCAGCGCGTACCGATTTTCATCACCACCAGGCCAATg                                        <  2:695723/140‑1 (MQ=255)                                                                                        aaaGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGACAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAGTCGTTCAATCCAGCGCGTACCGATTTTCATCACCACCAGGCCAATGa                                       <  2:18342/140‑1 (MQ=255)                                                                                        aaaGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGACAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAGTCGTTCAATCCAGCGCGTACCGATTTTCATCACCACCAGGCCAATGa                                       <  1:180958/140‑1 (MQ=255)                                                                                        aaaGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGACAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAGTCGTTCAATCCAGCGCGTACCGATTTTCATCACCACCAGGCCAATGa                                       <  1:19814/140‑1 (MQ=255)                                                                                             ccGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGACAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAGTCGTTCAATCCAGCGCGTACCGATTTTCATCACCACCAGGCCAATGa                                       >  1:588740/1‑135 (MQ=255)                                                                                             ccGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGACAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAGTCGTTCAATCCAGCGCGTACCGATTTTCATCACCACCAGGCCAATGa                                       <  2:588740/135‑1 (MQ=255)                                                                                                aaGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGACAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAGTCGTTCAATCCAGCGCGTACCGATTTTCATCACCACCAGGCCAATGACGGTATaa                               >  2:31085/1‑140 (MQ=255)                                                                                                      aTACACTTTTCACTGCGATAGGCGCTAAGTTCAGGACAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAGTCGTTCAATCCAGCGCGTACCGATTTTCATCACCACCAGGCCAATGACGGTATAAACCAGc                         <  2:144056/140‑1 (MQ=255)                                                                                                         cacTTTTCACTGCGATAGGCGCTAAGTTCAGGACAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAGTCGTTCAATCCAGCGCGTACCGATTTTCATCACCACCAGGCCAATGACGGTATAAACCAGCCca                      <  1:219787/140‑1 (MQ=255)                                                                                                         cacTTTTCACTGCGATAGGCGCTAAGTTCAGGACAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAGTCGTTCAATCCAGCGCGTACCGATTTTCATCACCACCAGGCCAATGACGGTATAAACCAGCCca                      <  1:7710/140‑1 (MQ=255)                                                                                                         cacTTTTCACTGCGATAGGCGCTAAGTTCAGGACAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAGTCGTTCAATCCAGCGCGTACCGATTTTCATCACCACCAGGCCAATGACGGTATAAACCAGCCca                      <  1:274601/140‑1 (MQ=255)                                                                                                               tCACTGCGATAGGCGCTAAGTTCAGGACAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAGTCGTTCAATCCAGCGCGTACCGATTTTCATCACCACCAGGCCAATGACGGTATAAACCAGCCCACAGGCg                <  2:674446/140‑1 (MQ=255)                                                                                                                cACTGCGATAGGCGCTAAGTTCAGGACAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAGTCGTTCAATCCAGCGCGTACCGATTTTCATCACCACCAGGCCAATGACGGTATAAACCAGCCCACAGGCGa               >  2:60690/1‑140 (MQ=255)                                                                                                                    gCGATAGGCGCTAAGTTCAGGACAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAGTCGTTCAATCCAGCGCGTACCGATTTTCATCACCACCAGGCCAATGACGGTATAAACCAGCCCACAGGCGATAAt           >  1:180940/1‑140 (MQ=255)                                                                                                                          ggCGCTAAGTTCAGGACAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAGTCGTTCAATCCAGCGCGTACCGATTTTCATCACCACCAGGCCAATGACGGTATAAACCAGCCCACAGGCGATAATCCCGcc     <  2:180940/140‑1 (MQ=255)                                                                                                                          ggCGCTAAGTTCAGGACAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAGTCGTTCAATCCAGCGCGTACCGATTTTCATCACCACCAGGCCAATGACGGTATAAACCAGCCCACAGGCGATAATCCCGcc     <  1:60690/140‑1 (MQ=255)                                                                                                                             gcTAAGTTCAGGACAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAGTCGTTCAATCCAGCGCGTACCGATTTTCATCACCACCAGGCCAATGACGGTATAAACCAGCCCACAGGCGATAATCCCGCCCAg  <  1:391276/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                GAGAATCAATAACCGCTGGATCATGCCACGGGTAAACACCGCCACCAGCCCAATACACAGCACCGTCATTACCGCCATCCAGCTATCAAAGGCCGAAGCCGATACACTTTTCACTGCGATAGGCGCTAAGTTCAGGCCAATCGCCATCACCACCGCACCCGTCACCACCGGCGGCATCAGTCGTTCAATCCAGCGCGTACCGATTTTCATCACCACCAGGCCAATGACGGTATAAACCAGCCCACAGGCGATAATCCCGCCCAG  >  NC_000913/1069240‑1069503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑