Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,367,291	C→A	G208W (GGG→TGG)	pspF ←	DNA‑binding transcriptional dual regulator PspF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,367,291	0	C	A	100.0%	257.9 / NA	73	G208W (GGG→TGG)	pspF	DNA‑binding transcriptional dual regulator PspF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (38/35);  total (38/35)

ATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCCCGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCATCAACATTATGTCGCTTTCGCGCTCGCGCAGTGGTGGCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCA  >  NC_000913/1367154‑1367424                                                                                                                                          |                                                                                                                                      aTCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACg                                                                                                                                     <  2:8096/140‑1 (MQ=255)     ataatGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGaa                                                                                                                                 >  2:498054/1‑140 (MQ=255)         tgatgTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACaga                                                                                                                             >  1:444176/1‑140 (MQ=255)         tgatgTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACaga                                                                                                                             >  1:970258/1‑140 (MQ=255)           atgTCATCAAGCGGATATTCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAgg                                                                                                                           >  1:1083523/1‑140 (MQ=255)           atgTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAgg                                                                                                                           >  1:422844/1‑140 (MQ=255)           atgTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAgg                                                                                                                           >  1:362288/1‑140 (MQ=255)           atgTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAgg                                                                                                                           >  1:773376/1‑140 (MQ=255)            tgTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGc                                                                                                                          <  1:1224600/140‑1 (MQ=255)              tcatcaAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAg                                                                                                                        >  2:1074657/1‑140 (MQ=255)                atcaAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCt                                                                                                                      >  2:1208160/1‑140 (MQ=255)                atcaAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCt                                                                                                                      >  2:429738/1‑140 (MQ=255)                atcaAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCt                                                                                                                      >  1:443586/1‑140 (MQ=255)                     gCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGAtt                                                                                                                 <  2:1172877/140‑1 (MQ=255)                        gATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACTATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTcc                                                                                                              <  2:197914/140‑1 (MQ=255)                        gATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTcc                                                                                                              >  2:218324/1‑140 (MQ=255)                         aTAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTccc                                                                                                             <  1:1074657/140‑1 (MQ=255)                           attCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGa                                                                                                           <  2:1083523/138‑1 (MQ=255)                           aaTCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGa                                                                                                           <  2:362288/140‑1 (MQ=255)                                    tGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTgg                                                                                                  <  2:970258/140‑1 (MQ=255)                                         tGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGc                                                                                             >  1:825859/1‑140 (MQ=255)                                          ggCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCa                                                                                            <  2:222296/140‑1 (MQ=255)                                          ggCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCa                                                                                            <  2:1053376/140‑1 (MQ=255)                                          ggCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCa                                                                                            <  1:58052/140‑1 (MQ=255)                                          ggCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCa                                                                                            <  2:392203/140‑1 (MQ=255)                                                   cTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCt                                                                                   <  2:444176/140‑1 (MQ=255)                                                    tGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTg                                                                                  <  2:422844/140‑1 (MQ=255)                                                     gAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGc                                                                                 >  2:1222435/1‑140 (MQ=255)                                                      aaCGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCt                                                                                   >  1:44183/1‑137 (MQ=255)                                                      aaCGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCt                                                                                   <  2:44183/137‑1 (MQ=255)                                                      aaCGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGcc                                                                                >  1:3804/1‑140 (MQ=255)                                                      aaCGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGcc                                                                                >  2:27524/1‑140 (MQ=255)                                                        cGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCAt                                                                              <  2:443586/140‑1 (MQ=255)                                                        cGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCAt                                                                              <  2:248981/140‑1 (MQ=255)                                                        cGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCAt                                                                              <  1:675071/140‑1 (MQ=255)                                                        cGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCAt                                                                              <  1:287802/140‑1 (MQ=255)                                                           tCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCa                                                                               <  1:217280/136‑1 (MQ=255)                                                           tCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCa                                                                               >  2:217280/1‑136 (MQ=255)                                                             caccacGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCATCAACa                                                                         >  1:532458/1‑140 (MQ=255)                                                              accacGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCATCAACAt                                                                        >  1:1051602/1‑140 (MQ=255)                                                                     ttttCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCATCAACATTATGTCg                                                                 >  1:263869/1‑140 (MQ=255)                                                                       ttCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCATCAACATTATg                                                                    <  1:905282/135‑1 (MQ=255)                                                                       ttCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCATCAACATTATg                                                                    >  2:905282/1‑135 (MQ=255)                                                                           aTTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCATCAACATTATGTCGCTTtcg                                                           <  1:1208160/140‑1 (MQ=255)                                                                                 gAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCATCAACATTATGTCGCTTtcgcgctcg                                                     >  1:1024866/1‑140 (MQ=255)                                                                                 gAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCATCAACATTATGTCGCTTtcgcgctcg                                                     >  1:121166/1‑140 (MQ=255)                                                                                      tttCCCTGCCAAAGATAATTGAGCAATGTTTCTCTGGCGCGCTCCGGAAACCACGGGAACAGAGGCAGCTTGATTTCACGACACATCTGGATGGCAAAGTATTCTGCCATCAACATTATGTCGCTTTCGCGCTCGCGCAg                                                >  2:1144982/1‑140 (MQ=255)                                                                                          ccGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCATCAACATTATGTCGCTTTCGCGCTCGCGCAgtggt                                            <  1:423703/140‑1 (MQ=255)                                                                                           cGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCATCAACATTATGTCGCTTtcgcgctcgc                                                    >  1:1212477/1‑131 (MQ=255)                                                                                           cGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCATCAACATTATGTCGCTTtcgcgctcgc                                                    <  2:1212477/131‑1 (MQ=255)                                                                                            ggCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCATCAACATTATGTCGCTTTCGCGCTCGCGCAGtggtgg                                          <  1:172859/140‑1 (MQ=255)                                                                                            ggCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCATCAACATTATGTCGCTTTCGCGCTCGCGCAGtggtgg                                          <  1:218324/140‑1 (MQ=255)                                                                                             gCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCATCAACATTATGTCGCTTTCGCGCTCGCGCAGtggtgg                                          >  1:486587/1‑139 (MQ=255)                                                                                             gCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCATCAACATTATGTCGCTTTCGCGCTCGCGCAGtggtgg                                          <  2:486587/139‑1 (MQ=255)                                                                                             gCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCATCAACATTATGTCGCTTTCGCGCTCGCGCAGTGGTGGc                                         >  2:913318/1‑140 (MQ=255)                                                                                             gCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCATCAACATTATGTCGCTTTCGCGCTCGCGCAGTGGTGGc                                         <  1:913318/140‑1 (MQ=255)                                                                                             gCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCATCAACATTATGTCGCTTTCGCGCTCGCGCAGTGGTGGc                                         >  2:1161692/1‑140 (MQ=255)                                                                                             gCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCATCAACATTATGTCGCTTTCGCGCTCGCGCAGTGGTGGc                                         <  1:1161692/140‑1 (MQ=255)                                                                                                aaCGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCATCAACATTATGTCGCTTTCGCGCTCGCGCAGTGGTGGCAGt                                      <  2:428666/140‑1 (MQ=255)                                                                                                  cGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCATCAACATTATGTCGCTTTCGCGCTCGCGCa                                                 <  2:14440/127‑1 (MQ=255)                                                                                                  cGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCATCAACATTATGTCGCTTTCGCGCTCGCGCa                                                 >  1:14440/1‑127 (MQ=255)                                                                                                        ttCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCATCAACATTATGTCGCTTTCGCGCTCGCGCAgtggt                                            >  1:946066/1‑126 (MQ=255)                                                                                                        ttCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCATCAACATTATGTCGCTTTCGCGCTCGCGCAgtggt                                            <  2:946066/126‑1 (MQ=255)                                                                                                           aGCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCATCAACATTATGTCGCTTTCGCGCTCGCGCAGTGGTGGCAGTTGTACAACATc                           >  2:1008217/1‑140 (MQ=255)                                                                                                            gCAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCATCAACATTATGTCGCTTTCGCGCTCGCGCAGTGGTGGCAGTTGTACAACATCa                          >  1:152094/1‑140 (MQ=255)                                                                                                             cAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCATCAACATTATGTCGCTTTCGCGCTCGCGCAGtggtgg                                          <  1:579759/123‑1 (MQ=255)                                                                                                             cAATGTTTCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCATCAACATTATGTCGCTTTCGCGCTCGCGCAGtggtgg                                          >  2:579759/1‑123 (MQ=255)                                                                                                                  tttCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCATCAACATTATGTCGCTTTCGCGCTCGCGCAGTGGTGGCAGTTGTACAACATCAAAAGcc                    >  1:600278/1‑140 (MQ=255)                                                                                                                   ttCTCTGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCATCAACATTATGTCGCTTTCGCGCTCGCGCAGTGGTGGCAGTTGTACAACATCAAAAGCCa                   <  1:1008217/140‑1 (MQ=255)                                                                                                                     ctctGGCGCGCTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCATCAACATTATGTCGCTTTCGCGCTCGCGCAGTGGTGGCAGTTGTACAACATCAAAAGCCAgt                 <  1:934136/140‑1 (MQ=255)                                                                                                                          gcgcgcTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCATCAACATTATGTCGCTTTCGCGCTCGCGCAGTGGTGGCAGTTGTACAACATCAAAAGCCAgtcggtc            >  2:140702/1‑140 (MQ=255)                                                                                                                           cgcgcTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCATCAACATTATGTCGCTTTCGCGCTCGCGCAGTGGTGGCAGTTGTACAACATCAAAAGCCAgtcggtcg           >  1:397092/1‑140 (MQ=255)                                                                                                                               cTCCGTAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCATCAACATTATGTCGCTTTCGCGCTCGCGCAGTGGTGGCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCa       >  2:205494/1‑140 (MQ=255)                                                                                                                                    tAAACCACGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCATCAACATTATGTCGCTTTCGCGCTCGCGCAGTGGTGGCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCa  >  2:50333/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      ATCAATAATGATGTCATCAAGCGGATAATCGCTGGTGCCGTGGCGATACACTGAACGTTCCACCACGTTTTTCAATTCACGAATATTTCCCGGCCAACGATAATTCAGCAATGTTTCTCTGGCGCGCTCCGTAAACCCCGGGAACAGAGGCAGCTTGATTTCCCGACACATCTGGATGGCAAAGTATTCTGCCATCAACATTATGTCGCTTTCGCGCTCGCGCAGTGGTGGCAGTTGTACAACATCAAAAGCCAGTCGGTCGAGCAGGTCA  >  NC_000913/1367154‑1367424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑