Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,024,541	G→T	V192F (GTC→TTC)	rcsA →	DNA‑binding transcriptional activator RcsA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,024,541	0	G	T	100.0%	320.0 / NA	89	V192F (GTC→TTC)	rcsA	DNA‑binding transcriptional activator RcsA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (46/43);  total (46/43)

TGCGAATGTGGATGGCAGGTCAGGGAACCATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGC  >  NC_000913/2024404‑2024676                                                                                                                                          |                                                                                                                                        tGCGAATGTGGATGGCAGGTCAGGGAACCATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATgtcttc                                                                                                                                       >  2:991490/1‑140 (MQ=255) tGCGAATGTGGATGGCAGGTCAGGGAACCATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATgtcttc                                                                                                                                       >  2:58667/1‑140 (MQ=255)    gAATGTGGATGGCAGGTCAGGGAACCATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGa                                                                                                                                    <  2:206166/140‑1 (MQ=255)      aTGTGGATGGCAGGTCAGGGAACCATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACt                                                                                                                                  <  1:211990/140‑1 (MQ=255)        gtgGATGGCAGGTCAGGGAACCATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGa                                                                                                                                >  2:119368/1‑140 (MQ=255)           gATGGCAGGTCAGGGAACCATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACgg                                                                                                                             >  2:1092503/1‑140 (MQ=255)                cAGGTCAGGGAACCATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACt                                                                                                                                  >  1:832009/1‑130 (MQ=255)                cAGGTCAGGGAACCATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACt                                                                                                                                  <  2:832009/130‑1 (MQ=255)                cAGGTCAGGGAACCATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAAt                                                                                                                        >  1:750658/1‑140 (MQ=255)                   gTCAGGGAACCATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAAtgtg                                                                                                                     >  1:823071/1‑140 (MQ=255)                     cAGGGAACCATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGAc                                                                                                                   <  2:162263/140‑1 (MQ=255)                        ggAACCATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTaa                                                                                                                >  2:907974/1‑140 (MQ=255)                          aaCCATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATg                                                                                                              <  2:376631/140‑1 (MQ=255)                            ccATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGt                                                                                                            >  2:311902/1‑140 (MQ=255)                             cATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTaa                                                                                                                >  2:472959/1‑135 (MQ=255)                             cATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTaa                                                                                                                <  1:472959/135‑1 (MQ=255)                             cATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTa                                                                                                           >  1:837542/1‑140 (MQ=255)                             cATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTa                                                                                                           >  2:1131821/1‑140 (MQ=255)                              aTTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATg                                                                                                              <  2:465410/136‑1 (MQ=255)                              aTTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATg                                                                                                              >  1:465410/1‑136 (MQ=255)                              aTTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTAt                                                                                                          <  1:1120462/140‑1 (MQ=255)                                  aaaTCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTAttttt                                                                                                      >  1:197363/1‑140 (MQ=255)                                   aaTCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTg                                                                                                     <  1:138783/140‑1 (MQ=255)                                    aTCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGt                                                                                                    <  1:754783/140‑1 (MQ=255)                                         tGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACa                                                                                               <  1:991490/140‑1 (MQ=255)                                         tGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACa                                                                                               <  1:633964/140‑1 (MQ=255)                                         tGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACa                                                                                               <  2:435460/140‑1 (MQ=255)                                         tGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACa                                                                                               <  1:119368/140‑1 (MQ=255)                                             cAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATgcg                                                                                           <  2:958926/140‑1 (MQ=255)                                             cAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATgcg                                                                                           <  1:461182/140‑1 (MQ=255)                                             cAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATgcg                                                                                           <  2:750658/140‑1 (MQ=255)                                                          aaGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTg                                                                              >  2:217596/1‑140 (MQ=255)                                                           aGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTAACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGa                                                                             >  1:249014/1‑140 (MQ=255)                                                               caGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACtgg                                                                         <  2:1033937/139‑1 (MQ=255)                                                               aaGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACtgg                                                                         >  1:580295/1‑140 (MQ=255)                                                                aGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACtggt                                                                        >  1:373829/1‑140 (MQ=255)                                                                  aCCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACtggtgg                                                                      <  2:373829/140‑1 (MQ=255)                                                                    cGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGtt                                                                    <  2:745643/140‑1 (MQ=255)                                                                            cGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCccacca                                                            >  1:406632/1‑140 (MQ=255)                                                                            cGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCccacca                                                            >  2:454095/1‑140 (MQ=255)                                                                                  aaGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAgg                                                      <  1:311902/140‑1 (MQ=255)                                                                                  aaGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAgg                                                      <  2:840656/140‑1 (MQ=255)                                                                                  aaGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAgg                                                      <  2:791122/140‑1 (MQ=255)                                                                                  aaGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAgg                                                      <  1:1131821/140‑1 (MQ=255)                                                                                       aaTATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGaa                                                 <  1:58667/140‑1 (MQ=255)                                                                                        atatTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGc                                                     >  2:239301/1‑135 (MQ=255)                                                                                        atatTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGc                                                     <  1:239301/135‑1 (MQ=255)                                                                                         tatTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATa                                               >  2:1073973/1‑140 (MQ=255)                                                                                         tatTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATa                                               >  1:1059847/1‑140 (MQ=255)                                                                                         tatTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATa                                               >  2:173068/1‑140 (MQ=255)                                                                                             aaaCGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGAt                                           >  2:1199166/1‑140 (MQ=255)                                                                                             aaaCGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGAt                                           >  1:813733/1‑140 (MQ=255)                                                                                              aaCGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTGTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGAtt                                          >  2:276866/1‑140 (MQ=255)                                                                                              aaCGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGAtt                                          >  2:323366/1‑140 (MQ=255)                                                                                              aaCGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGAtt                                          >  1:725532/1‑140 (MQ=255)                                                                                               aCGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGa                                            <  1:183453/137‑1 (MQ=255)                                                                                               aCGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGa                                            >  2:183453/1‑137 (MQ=255)                                                                                               aCGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTa                                         <  1:907974/140‑1 (MQ=255)                                                                                               aCGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTa                                         >  2:214778/1‑140 (MQ=255)                                                                                                    aGATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTg                                    >  1:709624/1‑140 (MQ=255)                                                                                                     gATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGa                                            <  2:771151/131‑1 (MQ=255)                                                                                                     gATCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGa                                            >  1:771151/1‑131 (MQ=255)                                                                                                      aTCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCt                                  <  2:184937/140‑1 (MQ=255)                                                                                                      aTCAAAACGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCt                                  <  2:837542/140‑1 (MQ=255)                                                                                                         aaaaCGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTtc                               <  2:197363/140‑1 (MQ=255)                                                                                                         aaaaCGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTtc                               <  1:84904/140‑1 (MQ=255)                                                                                                          aaaCGCATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTtct                              >  1:1221538/1‑140 (MQ=255)                                                                                                               cATAATAAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCcaca                         <  2:725532/140‑1 (MQ=255)                                                                                                                ataataAACAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACaa                        <  2:782921/140‑1 (MQ=255)                                                                                                                     aaaCAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATa                   <  1:1092503/140‑1 (MQ=255)                                                                                                                     aaaCAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATa                   <  2:80402/140‑1 (MQ=255)                                                                                                                     aaaCAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATa                   <  2:580295/140‑1 (MQ=255)                                                                                                                        cAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCg                >  1:24222/1‑140 (MQ=255)                                                                                                                        cAGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCg                <  1:217596/140‑1 (MQ=255)                                                                                                                         aGGTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGt               >  1:714522/1‑140 (MQ=255)                                                                                                                          ggTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTc              >  2:614338/1‑140 (MQ=255)                                                                                                                           gTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTcc             >  2:1083398/1‑140 (MQ=255)                                                                                                                           gTTATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTcc             <  1:1083398/140‑1 (MQ=255)                                                                                                                            ttATCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCt            >  1:1100768/1‑140 (MQ=255)                                                                                                                              aTCTACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGa          >  1:362639/1‑140 (MQ=255)                                                                                                                                 tACCATGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGc       >  2:414929/1‑140 (MQ=255)                                                                                                                                     aTGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTg   >  1:853074/1‑140 (MQ=255)                                                                                                                                     aTGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTg   >  1:486453/1‑140 (MQ=255)                                                                                                                                     aTGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTg   >  1:848650/1‑140 (MQ=255)                                                                                                                                     aTGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTg   >  1:60752/1‑140 (MQ=255)                                                                                                                                      tGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCt            <  2:568546/130‑1 (MQ=255)                                                                                                                                      tGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCt            >  1:568546/1‑130 (MQ=255)                                                                                                                                      tGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGc  >  2:56366/1‑140 (MQ=255)                                                                                                                                      tGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGc  >  1:1055173/1‑140 (MQ=255)                                                                                                                                      tGTCTTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGc  <  2:1221538/140‑1 (MQ=255)                                                                                                                                      tGTCTTCCGACTGACGGATAATGTAACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGc  <  2:249014/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        TGCGAATGTGGATGGCAGGTCAGGGAACCATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTGACTAATGGTATTTTTGTCAACATGCGCTAACACATTCTGACTGGTGGTTTCCCACCAGTCAGGCTGAATAAGATTACTCTGCTTTCTCCACAAAGATACCGTCCTGATGCCCTGC  >  NC_000913/2024404‑2024676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑