Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,339,065	G→T	A119E (GCA→GAA)	gyrA ←	DNA gyrase subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,339,065	0	G	T	100.0%	168.6 / NA	49	A119E (GCA→GAA)	gyrA	DNA gyrase subunit A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (13/36);  total (13/36)

GGAATTTTGGTTGGCATGACGTCCGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTAT  >  NC_000913/2338930‑2339193                                                                                                                                        |                                                                                                                                 ggAATTTTGGTTGGCATGACGTCCGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTtccgc                                                                                                                              >  2:1096291/1‑140 (MQ=255)           ttggCATGACGTCCGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCg                                                                                                                    <  1:186511/140‑1 (MQ=255)           ctggCATGACGTCCGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCg                                                                                                                    <  1:894123/139‑1 (MQ=255)             ggCATGACGTCCGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGcc                                                                                                                  <  1:141348/140‑1 (MQ=255)                            aTTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGaa                                                                                                   <  2:867616/140‑1 (MQ=255)                            aTTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGaa                                                                                                   <  1:617638/140‑1 (MQ=255)                                     gTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTg                                                                                          >  2:666017/1‑140 (MQ=255)                                        ccGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGAcc                                                                                       <  2:91099/140‑1 (MQ=255)                                           tCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTc                                                                                    <  2:57901/140‑1 (MQ=255)                                           tCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTc                                                                                    <  1:683730/140‑1 (MQ=255)                                              tAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGCACCGAAGTTACCCTGACCGTCTAc                                                                                 <  2:679287/140‑1 (MQ=255)                                              tAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTAc                                                                                 <  2:270114/140‑1 (MQ=255)                                              tAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTAc                                                                                 <  1:769300/140‑1 (MQ=255)                                              tAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGACAATTTTCGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTAc                                                                                 <  2:170578/140‑1 (MQ=255)                                               aGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTAcc                                                                                <  1:182401/140‑1 (MQ=255)                                                  tATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGc                                                                             <  2:576989/140‑1 (MQ=255)                                                   aTCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCa                                                                            >  2:1140278/1‑140 (MQ=255)                                                   aTCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCa                                                                            >  2:643612/1‑140 (MQ=255)                                                    tCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCat                                                                           <  1:331595/140‑1 (MQ=255)                                                     cAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCa                                                                               >  1:4028/1‑135 (MQ=255)                                                     cAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCa                                                                               <  2:4028/135‑1 (MQ=255)                                                     cAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCata                                                                          >  1:373045/1‑140 (MQ=255)                                                     cAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCata                                                                          >  1:1076937/1‑140 (MQ=255)                                                         gAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAAc                                                                      >  2:483166/1‑140 (MQ=255)                                                                aCCGTCTCTTTTTCGAGATCGGCCATCAGTTCACGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCga                                                               >  1:1071750/1‑140 (MQ=255)                                                                 ccGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCgag                                                              <  1:32240/140‑1 (MQ=255)                                                                   gTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGaa                                                            >  1:378501/1‑140 (MQ=255)                                                                         ttttCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAATGCAGCGAGAATGGCTg                                                      <  2:775728/140‑1 (MQ=255)                                                                         ttttCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTg                                                      <  1:531249/140‑1 (MQ=255)                                                                           ttCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTgcg                                                    >  2:971614/1‑140 (MQ=255)                                                                                       cATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGa                                        <  2:695288/140‑1 (MQ=255)                                                                                         tCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATc                                      <  2:996498/140‑1 (MQ=255)                                                                                         tCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATc                                      <  1:34485/140‑1 (MQ=255)                                                                                         tCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATc                                      <  1:643612/140‑1 (MQ=255)                                                                                         tCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATc                                      <  1:76840/140‑1 (MQ=255)                                                                                                  gggCAATTTTCGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAg                             >  1:653876/1‑140 (MQ=255)                                                                                                    gCAATTTTCGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGAc                           <  1:698577/140‑1 (MQ=255)                                                                                                       aTTTTCGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGAccgc                        <  2:373045/140‑1 (MQ=255)                                                                                                            cGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGt                   <  1:1096291/140‑1 (MQ=255)                                                                                                            cGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGt                   <  1:164009/140‑1 (MQ=255)                                                                                                            cGCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGt                   <  1:666017/140‑1 (MQ=255)                                                                                                             gCCAGACGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTc                  >  1:756450/1‑140 (MQ=255)                                                                                                                  aCGGATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCAt             <  2:378501/140‑1 (MQ=255)                                                                                                                    ggATTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATgg           <  1:819608/140‑1 (MQ=255)                                                                                                                      aTTTCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGATTCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATgggg         <  1:241458/140‑1 (MQ=255)                                                                                                                       tttCCGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGa        <  2:1076937/140‑1 (MQ=255)                                                                                                                          ccGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATgg     <  2:653876/140‑1 (MQ=255)                                                                                                                           cGTATAACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGt    <  1:1140278/140‑1 (MQ=255)                                                                                                                            gTATAACGCATTTCCGCCGCAGAGTCGCCGTCCATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTa   >  2:277468/1‑140 (MQ=255)                                                                                                                             tataACGCATTTCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTAt  <  2:47512/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                 GGAATTTTGGTTGGCATGACGTCCGGAATTTTTTCCGTGCCGTCATAGTTATCAACGAAATCGACCGTCTCTTTTTCGAGATCGGCCATCAGTTCATGGGCAATTTTCGCCAGACGGATTTCCGTATAACGCATTGCCGCCGCAGAGTCGCCGTCGATAGAACCGAAGTTACCCTGACCGTCTACCAGCATATAACGCAGCGAGAATGGCTGCGCCATGCGGACGATCGTGTCATAGACCGCCGAGTCACCATGGGGATGGTAT  >  NC_000913/2338930‑2339193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑