Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,484,466	C→A	I31I (ATC→ATA)	evgS →	sensory histidine kinase EvgS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,484,466	0	C	A	100.0%	340.8 / NA	94	I31I (ATC→ATA)	evgS	sensory histidine kinase EvgS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (49/45);  total (49/45)

TCTTTACACATTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATCAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAACGGCTACGTTGTTGCATACCGATTC  >  NC_000913/2484333‑2484597                                                                                                                                      |                                                                                                                                    tCTTTACACATTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAagtagt                                                                                                                               <  2:1107494/140‑1 (MQ=255)    ttACACATTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTaac                                                                                                                            >  1:1060909/1‑140 (MQ=255)         caTTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCg                                                                                                                       <  2:989676/140‑1 (MQ=255)          aTTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTaac                                                                                                                            >  2:196104/1‑134 (MQ=255)          aTTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTaac                                                                                                                            <  1:196104/134‑1 (MQ=255)          aTTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCgt                                                                                                                      >  1:822078/1‑140 (MQ=255)           ttCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCgtg                                                                                                                     <  1:1194337/140‑1 (MQ=255)           ttCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCgtg                                                                                                                     >  1:449325/1‑140 (MQ=255)           ttCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCgtg                                                                                                                     >  1:792167/1‑140 (MQ=255)            tCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCgtgt                                                                                                                    >  2:798825/1‑140 (MQ=255)            tCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCgtgt                                                                                                                    >  1:524736/1‑140 (MQ=255)            tCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCgtgt                                                                                                                    <  1:701943/140‑1 (MQ=255)            tCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCgtgt                                                                                                                    >  1:287799/1‑140 (MQ=255)            tCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCgtgt                                                                                                                    >  2:701943/1‑140 (MQ=255)             cGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTc                                                                                                                   <  1:267269/140‑1 (MQ=255)               cacaACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTcac                                                                                                                 >  2:1034012/1‑140 (MQ=255)                  aaCGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACt                                                                                                              <  1:160012/140‑1 (MQ=255)                    cGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTg                                                                                                            >  2:295858/1‑140 (MQ=255)                    cGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTg                                                                                                            >  1:345686/1‑140 (MQ=255)                     gTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGa                                                                                                           <  2:316285/140‑1 (MQ=255)                     gTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGa                                                                                                           <  1:827283/140‑1 (MQ=255)                     gTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGa                                                                                                           <  1:372087/140‑1 (MQ=255)                      tAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGAt                                                                                                          >  2:1110501/1‑140 (MQ=255)                       aaCAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATc                                                                                                         <  1:1110501/140‑1 (MQ=255)                        aCAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATcc                                                                                                        >  1:868826/1‑140 (MQ=255)                          aaaaTCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCAc                                                                                                      <  1:1034012/140‑1 (MQ=255)                          aaaaTCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCAc                                                                                                      <  2:1060909/140‑1 (MQ=255)                          aaaaTCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCAc                                                                                                      <  1:1125046/140‑1 (MQ=255)                              tCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGa                                                                                                           <  1:998620/131‑1 (MQ=255)                              tCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGa                                                                                                           >  2:998620/1‑131 (MQ=255)                              tCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGa                                                                                                           <  1:258520/131‑1 (MQ=255)                              tCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACg                                                                                                  >  2:720392/1‑140 (MQ=255)                              tCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACg                                                                                                  >  2:849922/1‑140 (MQ=255)                              tCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACg                                                                                                  >  1:724387/1‑140 (MQ=255)                              tCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTATGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGa                                                                                                           >  2:258520/1‑131 (MQ=255)                               cGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGCGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGt                                                                                                 >  1:17302/1‑140 (MQ=255)                                 gCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCt                                                                                               >  2:1142742/1‑140 (MQ=255)                                  cTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTg                                                                                              <  2:1055418/140‑1 (MQ=255)                                         caTGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGcaaca                                                                                       <  1:151828/140‑1 (MQ=255)                                         caTGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGCGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGcaaca                                                                                       <  2:17302/140‑1 (MQ=255)                                                 ttttACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTa                                                                               >  2:704861/1‑140 (MQ=255)                                                   ttACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACg                                                                             <  2:932954/140‑1 (MQ=255)                                                         tataTTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGtt                                                                       >  1:660595/1‑140 (MQ=255)                                                         tataTTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGtt                                                                       >  1:516954/1‑140 (MQ=255)                                                           tatTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAg                                                                     >  2:456989/1‑140 (MQ=255)                                                                   ttctCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCaaa                                                             >  1:278795/1‑140 (MQ=255)                                                                     ctctGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCaaaaa                                                           >  1:734422/1‑140 (MQ=255)                                                                     ctctGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCaaaaa                                                           >  1:756749/1‑140 (MQ=255)                                                                     ctctGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCaaaaa                                                           >  1:802991/1‑140 (MQ=255)                                                                     ctctGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCaaaaa                                                           >  1:274509/1‑140 (MQ=255)                                                                     ctctGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCaaaaa                                                           >  2:646094/1‑140 (MQ=255)                                                                          ttgtGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATc                                                      >  1:657744/1‑140 (MQ=255)                                                                          ttgtGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGAAAAAAAAATc                                                      >  2:189059/1‑140 (MQ=255)                                                                           tgtgGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCt                                                     <  1:113221/140‑1 (MQ=255)                                                                           tgtgGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCt                                                     <  2:868826/140‑1 (MQ=255)                                                                              ggTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTtgt                                                  <  1:456989/140‑1 (MQ=255)                                                                                tCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGa                                                <  1:803035/140‑1 (MQ=255)                                                                                 cTTTGGTCGACCATAAGTTTCGCAGACGAAGATTTCATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGAt                                               <  1:398481/140‑1 (MQ=255)                                                                                 cTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGAt                                               <  1:897778/140‑1 (MQ=255)                                                                                 cTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGAt                                               <  2:1162131/140‑1 (MQ=255)                                                                                  tttGGTCGACCTTAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGAtt                                              >  2:114104/1‑140 (MQ=255)                                                                                  tttGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGAtt                                              >  2:602714/1‑140 (MQ=255)                                                                                  tttGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGAtt                                              >  1:639394/1‑140 (MQ=255)                                                                                   ttGGTCGACCATTAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTg                                             <  2:238355/140‑1 (MQ=255)                                                                                   ttGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTg                                             <  2:657744/140‑1 (MQ=255)                                                                                   ttGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTg                                             <  2:876178/140‑1 (MQ=255)                                                                                   ttGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTg                                             <  2:1168378/140‑1 (MQ=255)                                                                                   ttGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTg                                             <  2:413594/140‑1 (MQ=255)                                                                                   ttGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTg                                             <  1:114104/140‑1 (MQ=255)                                                                                   ttGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTg                                             <  1:418139/140‑1 (MQ=255)                                                                                    tGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGc                                            >  1:1069369/1‑140 (MQ=255)                                                                                    tGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGc                                            >  1:867365/1‑140 (MQ=255)                                                                                           ccATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACAt                                     <  1:192980/140‑1 (MQ=255)                                                                                                     cGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCaaa                           >  1:159720/1‑140 (MQ=255)                                                                                                     cGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCaaa                           >  1:795886/1‑140 (MQ=255)                                                                                                     cGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCaaa                           >  1:235515/1‑140 (MQ=255)                                                                                                     cGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCaaa                           >  2:50892/1‑140 (MQ=255)                                                                                                     cGCAGACGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCaaa                           >  2:1174925/1‑140 (MQ=255)                                                                                                           cGAAGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAACGGCTa                     <  1:704861/140‑1 (MQ=255)                                                                                                             aaGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAACGGCTACg                   <  2:516954/140‑1 (MQ=255)                                                                                                             aaGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAACGGCTACg                   <  2:639394/140‑1 (MQ=255)                                                                                                             aaGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAACGGCTACg                   <  1:189059/140‑1 (MQ=255)                                                                                                             aaGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAACGGCTACg                   <  1:616809/140‑1 (MQ=255)                                                                                                             aaGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAACGGCTACg                   <  1:798825/140‑1 (MQ=255)                                                                                                             aaGATTACATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAACGGCTACg                   <  2:724387/140‑1 (MQ=255)                                                                                                                    cATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAACGGCTa                     <  1:108299/131‑1 (MQ=255)                                                                                                                    cATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAACGGCTa                     >  2:108299/1‑131 (MQ=255)                                                                                                                    cATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAACGGCTACGTTGTTGc            >  2:575024/1‑140 (MQ=255)                                                                                                                    cATCGAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAACGGCTACGTTGTTGc            >  1:374052/1‑140 (MQ=255)                                                                                                                        gAATATCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAACGGCTACGTTGTTGCATAc        <  1:644394/140‑1 (MQ=255)                                                                                                                            atCGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAACGGCTACGTTGTTGCATACCGAt    >  2:931223/1‑140 (MQ=255)                                                                                                                              cGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAACGGCTACGTTGTTGCATACCGATTc  <  2:345686/140‑1 (MQ=255)                                                                                                                              cGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAACGGCTACGTTGTTGCATACCGATTc  >  2:799285/1‑140 (MQ=255)                                                                                                                              cGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAACGGCTACGTTGTTGCATACCGATTc  >  2:52788/1‑140 (MQ=255)                                                                                                                              cGTGGCATAAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAACGGCTACGTTGTTGCATACCGATTc  >  1:49045/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                    TCTTTACACATTCGCACAACGTAACAAAATCGGCTAACCACATGAAGTTTTTACCCTATATTTTTCTTCTCTGTTGTGGTCTTTGGTCGACCATAAGTTTCGCAGACGAAGATTACATCGAATATCGTGGCATCAGTAGTAACAACCGTGTCACACTTGATCCACTACGTCTGAGCAACAAGGAATTACGTTGGTTAGCGAGCAAAAAAAATCTTGTGATTGCAGTACATAAGTCCCAAACGGCTACGTTGTTGCATACCGATTC  >  NC_000913/2484333‑2484597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑