Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,355,158	C→A	P145Q (CCG→CAG)	gltB →	glutamate synthase subunit GltB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,355,158	0	C	A	100.0%	163.1 / NA	46	P145Q (CCG→CAG)	gltB	glutamate synthase subunit GltB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (24/22);  total (24/22)

CGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGAACATCTATAAAGGT  >  NC_000913/3355022‑3355288                                                                                                                                         |                                                                                                                                   cgcATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCAGGc                                                                                                                                 >  1:81737/1‑140 (MQ=255)    aTCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCcaggcagg                                                                                                                              <  1:744735/140‑1 (MQ=255)           aagaagaaCTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGt                                                                                                                       <  1:1145068/140‑1 (MQ=255)           aagaagaaCTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGt                                                                                                                       <  1:624627/140‑1 (MQ=255)             gaagaaCTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTcc                                                                                                                     <  1:659485/140‑1 (MQ=255)                   cTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTCCACGCGa                                                                                                               >  2:622080/1‑140 (MQ=255)                   cTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTCCACGCGa                                                                                                               >  2:833570/1‑140 (MQ=255)                   cTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTCCACGCGa                                                                                                               >  1:705278/1‑140 (MQ=255)                           cgAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAgc                                                                                                       >  2:967826/1‑140 (MQ=255)                                              ggCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATcgcccg                                                                                    >  1:53116/1‑140 (MQ=255)                                                   gCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCccgccgcc                                                                               <  1:258964/140‑1 (MQ=255)                                                             cccACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGaaaa                                                                     >  1:598018/1‑140 (MQ=255)                                                             cccACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGaaaa                                                                     >  2:363952/1‑140 (MQ=255)                                                                     cGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCg                                                             >  1:589279/1‑140 (MQ=255)                                                                     cGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCg                                                             >  2:91545/1‑140 (MQ=255)                                                                       aaGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGaa                                                           <  1:1011442/140‑1 (MQ=255)                                                                            gTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGa                                                      >  1:532497/1‑140 (MQ=255)                                                                            gTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGa                                                      >  1:1163682/1‑140 (MQ=255)                                                                             tGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGAc                                                     >  2:977438/1‑140 (MQ=255)                                                                             tGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGAc                                                     >  2:123822/1‑140 (MQ=255)                                                                              gCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACa                                                    >  1:902668/1‑140 (MQ=255)                                                                              gCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACa                                                    >  1:650330/1‑140 (MQ=255)                                                                                    tGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACt                                              <  1:784394/140‑1 (MQ=255)                                                                                            cccTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTc                                            >  1:171637/1‑134 (MQ=255)                                                                                            cccTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTc                                            <  2:171637/134‑1 (MQ=255)                                                                                                ctcctctcTGCCACGCATTGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTa                                  <  2:705278/140‑1 (MQ=255)                                                                                                 tcctctcTGCCACGCATTGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAg                                 >  2:678115/1‑140 (MQ=255)                                                                                                  cctctcTGCCACGCATTGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGc                                <  1:716536/140‑1 (MQ=255)                                                                                                  cctctcTGCCACGCATTGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAGCGACGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGc                                <  1:967826/140‑1 (MQ=255)                                                                                                    tctctGCCACGCATTGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCt                              <  1:833570/140‑1 (MQ=255)                                                                                                      tctGCCACGCATTGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGt                            <  2:414571/140‑1 (MQ=255)                                                                                                         gCCACGCATTGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGa                         <  1:91545/140‑1 (MQ=255)                                                                                                           cACGCATTGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAAt                       >  2:820780/1‑140 (MQ=255)                                                                                                           cACGCATTGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAAt                       >  1:1184609/1‑140 (MQ=255)                                                                                                           cACGCATTGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAAt                       >  2:404982/1‑140 (MQ=255)                                                                                                           cACGCATTGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAAt                       <  1:363952/140‑1 (MQ=255)                                                                                                             cGCATTGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCt                     >  2:351558/1‑140 (MQ=255)                                                                                                               cATTGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTgg                   >  2:995164/1‑140 (MQ=255)                                                                                                                 ttGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTg                 <  2:131467/140‑1 (MQ=255)                                                                                                                 ttGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTg                 <  2:902668/140‑1 (MQ=255)                                                                                                                 ttGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTg                 <  2:1163682/140‑1 (MQ=255)                                                                                                                 ttGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTg                 <  1:995164/140‑1 (MQ=255)                                                                                                                  tGAGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGa                >  2:1172695/1‑140 (MQ=255)                                                                                                                    aGCAAATTTTTGTGAACGCCCAGGCAGGCTGGTGTCCACGCGATATGGAGCGCCGTCTGTGTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGAAc              <  2:1197054/140‑1 (MQ=255)                                                                                                                    aGCAAATTTTTGTGAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGAAc              <  2:589279/140‑1 (MQ=255)                                                                                                                           ttttGTGAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGAACATCtata       <  2:732342/140‑1 (MQ=255)                                                                                                                                tgAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGAACATCTATAAAGGt  <  2:968796/140‑1 (MQ=255)                                                                                                                                tgAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGAACATCTATAAAGGt  >  1:968796/1‑140 (MQ=255)                                                                                                                                tgAACGCCCAGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGAACATCTATAAAGGt  <  1:820780/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                   CGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTCTCTGCCACGCATTGAGCAAATTTTTGTGAACGCCCCGGCAGGCTGGCGTCCACGCGATATGGAGCGCCGTCTGTTTATCGCCCGCCGCCGCATTGAAAAGCGTCTCGAAGCCGACAAAGACTTCTACGTCTGTAGCCTGTCGAATCTGGTGAACATCTATAAAGGT  >  NC_000913/3355022‑3355288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑