| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,472,003 | G→T | G131G (GGC→GGA) | pyrB ← | aspartate carbamoyltransferase catalytic subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,472,003 | 0 | G | T | 97.9% | 157.6 / ‑3.9 | 47 | G131G (GGC→GGA) | pyrB | aspartate carbamoyltransferase catalytic subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (23/23); minor base C (1/0); total (24/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01 | |||||||||||
GAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTC > NC_000913/4471873‑4472137 | gAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCg > 2:411647/1‑140 (MQ=255) gggAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTc < 2:918496/140‑1 (MQ=255) gggAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTc < 2:564168/140‑1 (MQ=255) gggAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTc < 2:236788/140‑1 (MQ=255) gAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAg < 1:1199877/140‑1 (MQ=255) gAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAg < 1:697476/140‑1 (MQ=255) aGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAGt < 2:240226/140‑1 (MQ=255) aaCGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCAc < 1:237264/125‑1 (MQ=255) aaCGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCAc > 2:237264/1‑125 (MQ=255) aaCGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAGTACCg > 1:27915/1‑140 (MQ=255) gTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAg < 1:1101581/131‑1 (MQ=255) gTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAg > 2:1101581/1‑131 (MQ=255) gCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAGTACCGGTa < 1:1124300/137‑1 (MQ=255) gCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAGTACCGGTa > 2:1124300/1‑137 (MQ=255) gCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAGTACCGGTACAt < 1:488352/140‑1 (MQ=255) cAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACt > 1:945431/1‑140 (MQ=255) ggTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCg < 1:248744/140‑1 (MQ=255) cACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTg > 2:1138450/1‑140 (MQ=255) ccaTTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAgg < 2:126918/140‑1 (MQ=255) gACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGCTGTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGcgccg < 2:763219/140‑1 (MQ=255) tGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCAcc > 1:574306/1‑140 (MQ=255) tGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGc > 2:187251/1‑140 (MQ=255) gACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGa < 1:1017257/140‑1 (MQ=255) gACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGa > 1:649677/1‑140 (MQ=255) cccTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCAt < 2:611622/140‑1 (MQ=255) ccTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGa < 2:355758/134‑1 (MQ=255) ccTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGa > 1:355758/1‑134 (MQ=255) ccTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATc > 1:765332/1‑140 (MQ=255) ccTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATc > 1:799058/1‑140 (MQ=255) ccTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGACCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATc > 2:369434/1‑140 (MQ=255) ggTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTa < 1:135453/140‑1 (MQ=255) tCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGc < 1:1138450/140‑1 (MQ=255) ccTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCa > 1:437663/1‑140 (MQ=255) aGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGt < 2:799058/140‑1 (MQ=255) tGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGTCGTaa > 2:305846/1‑140 (MQ=255) tGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTaa > 1:305056/1‑140 (MQ=255) tGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTaa > 1:783874/1‑140 (MQ=255) aaTAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGt > 1:576731/1‑140 (MQ=255) aaGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCt > 1:26492/1‑140 (MQ=255) aGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTg > 1:802508/1‑140 (MQ=255) gTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTgg > 1:827830/1‑139 (MQ=255) tCCAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGAt > 1:760400/1‑140 (MQ=255) cAGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATaa < 2:783874/140‑1 (MQ=255) aGCAAGGTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAAc < 1:573273/140‑1 (MQ=255) ggTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGaaa < 2:656045/140‑1 (MQ=255) gTTTGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAAt > 2:1016950/1‑140 (MQ=255) tttGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGAAGTAAGTGCTGATAACCGaa < 2:697736/137‑1 (MQ=255) tttGCGTCGGATGTTGGTTGGATCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGAAGTAAGTGCTGATAACCGaa > 1:697736/1‑137 (MQ=255) ttggATCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTc < 2:802508/140‑1 (MQ=255) | GAGTCAGGGAGTGAACGGTGCGGCCATATTTCAGGTCACCAACCATTGCGACGTGGAGATTGTCCAGACGCCCCTGGGTTTCCTGAATAGTGAATAAGTCCAGCAAGGTTTGCGTCGGATGTTGGTTGGAGCCATCACCGGCATTCAGTACCGGTACATTGCCGGAAAACTCGGTGGCCAGGCGCGCCGCACCTTCCTGCGGATGACGCATCACTATCGCATCGACGTAAGTGCTGATAACCGAAATGGTATCGGCCAGCGTTTC > NC_000913/4471873‑4472137 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |