BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,229,655	A→G	I247T (ATA→ACA)	hlyE ←	hemolysin E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,229,655	0	A	G	99.2%	432.8 / ‑4.6	117	I247T (ATA→ACA)	hlyE	hemolysin E
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (58/58); total (59/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.80e-01

Only 100 of 118 total aligned reads displayed.

GTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTAACCGTGTTAGACAGGGTGGTAAAGAAATTCTGCACAGATTTTAACTTGTTCTTCAATTCTGGAATCAGTTTTCCTTCAACTAC  >  NC_000913/1229519‑1229791

gtgtCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGttt                                                                                                                                       >  2:239417/1‑140 (MQ=255)

       ttCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCggtggt                                                                                                                                >  2:397358/1‑140 (MQ=255)

        tCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGtt                                                                                                                               >  1:465457/1‑140 (MQ=255)

            aTACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAAtt                                                                                                                           >  1:234494/1‑140 (MQ=255)

            aTACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAAtt                                                                                                                           >  1:1389459/1‑140 (MQ=255)

             tACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCg                                                                                                                                     <  1:1014408/129‑1 (MQ=255)

             tACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAAttt                                                                                                                          >  1:726990/1‑140 (MQ=255)

             tACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAAttt                                                                                                                          >  2:1435385/1‑140 (MQ=255)

                  aTTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAAtt                                                                                                                     >  1:130508/1‑140 (MQ=255)

                  aTTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAAtt                                                                                                                     >  2:130578/1‑140 (MQ=255)

                  aTTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAAtt                                                                                                                     >  2:1257237/1‑140 (MQ=255)

                  aTTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCACCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAAtt                                                                                                                     >  1:610827/1‑140 (MQ=255)

                    tACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTg                                                                                                                   >  2:1138444/1‑140 (MQ=255)

                       aGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTggcg                                                                                                                <  1:881210/140‑1 (MQ=255)

                       aGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTggcg                                                                                                                <  1:996865/140‑1 (MQ=255)

                       aGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTAGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTTCTTCGGTGGTTACTGTCAAGTTggcg                                                                                                                <  2:312971/140‑1 (MQ=255)

                        ggTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGTCTGTTTCGGTGGTTAATTTCAATTTggcgg                                                                                                               >  2:1098925/1‑140 (MQ=255)

                         gtgtTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTGAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTggcggc                                                                                                              >  2:464400/1‑140 (MQ=255)

                         gtgtTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTggcggc                                                                                                              <  1:31426/140‑1 (MQ=255)

                         gtgtTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTggcggc                                                                                                              <  1:965107/140‑1 (MQ=255)

                         gtgtTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTCAATTTCAATTTggcggc                                                                                                              >  1:603750/1‑140 (MQ=255)

                          tgtTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCa                                                                                                             >  2:517231/1‑140 (MQ=255)

                          tgtTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCa                                                                                                             >  1:1047805/1‑140 (MQ=255)

                          tgtTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCa                                                                                                             <  1:1014059/140‑1 (MQ=255)

                           gtTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCAt                                                                                                            <  2:234494/140‑1 (MQ=255)

                            ttAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTgg                                                                                                                  <  2:482798/133‑1 (MQ=255)

                            ttAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTgg                                                                                                                  >  1:482798/1‑133 (MQ=255)

                            ttAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATc                                                                                                           >  2:519376/1‑140 (MQ=255)

                            ttAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATc                                                                                                           >  1:80457/1‑140 (MQ=255)

                            ttAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATc                                                                                                           >  1:811905/1‑140 (MQ=255)

                            ttAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATc                                                                                                           >  1:142734/1‑140 (MQ=255)

                            ttAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATc                                                                                                           >  1:238181/1‑140 (MQ=255)

                            ttAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATc                                                                                                           >  2:898420/1‑140 (MQ=255)

                            ttAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATc                                                                                                           >  2:192362/1‑140 (MQ=255)

                            ttAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATc                                                                                                           >  1:1371271/1‑140 (MQ=255)

                            ttAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATc                                                                                                           >  2:654689/1‑140 (MQ=255)

                              aaTCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGa                                                                                                         >  2:260361/1‑140 (MQ=255)

                              aaTCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGa                                                                                                         >  1:929091/1‑140 (MQ=255)

                               aTCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGat                                                                                                        <  2:74856/140‑1 (MQ=255)

                                tCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGata                                                                                                       <  1:1428068/140‑1 (MQ=255)

                                   tttttttGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCt                                                                                                    >  2:898628/1‑140 (MQ=255)

                                     tttttGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCttt                                                                                                  <  1:941445/140‑1 (MQ=255)

                                      ttttGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTa                                                                                                 <  1:192362/140‑1 (MQ=255)

                                      ttttGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTa                                                                                                 >  1:676441/1‑140 (MQ=255)

                                         tGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTc                                                                                              <  2:80457/140‑1 (MQ=255)

                                           gCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGc                                                                                            <  1:1036627/140‑1 (MQ=255)

                                           gCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGc                                                                                            <  2:465457/140‑1 (MQ=255)

                                           gCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTCAATTTCAATTTGGCGGCATCGATATCTTTATTCGc                                                                                            <  2:603750/140‑1 (MQ=255)

                                              gCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCCCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTg                                                                                         >  2:30839/1‑140 (MQ=255)

                                               cttcttTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGt                                                                                        <  2:151978/140‑1 (MQ=255)

                                                   ttttAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTaa                                                                                    <  1:1428700/140‑1 (MQ=255)

                                                   ttttAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTaa                                                                                    <  2:565084/140‑1 (MQ=255)

                                                   ttttAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTaa                                                                                    <  2:594255/140‑1 (MQ=255)

                                                   ttttAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTaa                                                                                    <  1:130578/140‑1 (MQ=255)

                                                   ttttAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTaa                                                                                    <  1:1142061/140‑1 (MQ=255)

                                                   ttttAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTaa                                                                                    <  1:404825/140‑1 (MQ=255)

                                                   ttttAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTaa                                                                                    <  2:929091/140‑1 (MQ=255)

                                                   ttttAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTaa                                                                                    <  1:519376/140‑1 (MQ=255)

                                                   ttttAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTaa                                                                                    <  1:654689/140‑1 (MQ=255)

                                                   ttttAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTaa                                                                                    <  1:414571/140‑1 (MQ=255)

                                                   ttttAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTaa                                                                                    <  1:898628/140‑1 (MQ=255)

                                                   ttttAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTaa                                                                                    <  2:811905/140‑1 (MQ=255)

                                                   ttttAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCCCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTaa                                                                                    <  1:30839/140‑1 (MQ=255)

                                                   ttgtAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCTCCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTaa                                                                                    <  2:61099/137‑1 (MQ=255)

                                                          aaagaaagCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGc                                                                                            <  2:1189066/125‑1 (MQ=255)

                                                          aaagaaagCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGc                                                                                            >  1:1189066/1‑125 (MQ=255)

                                                            agaaagCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTAACCGTGTTAg                                                                           >  2:1325841/1‑140 (MQ=255)

                                                             gaaagCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTAACCGTGTTAGa                                                                          <  1:366025/140‑1 (MQ=255)

                                                             gaaagCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTAACCGTGTTAGa                                                                          <  1:1138444/140‑1 (MQ=255)

                                                             gaaagCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTAACCGTGTTAGa                                                                          <  2:238181/140‑1 (MQ=255)

                                                             gaaagCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTAACCGTGTTAGa                                                                          <  1:1257237/140‑1 (MQ=255)

                                                             gaaagCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTAACCGTGTTAGa                                                                          <  2:676441/140‑1 (MQ=255)

                                                             gaaagCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTAACCGTGTTAGa                                                                          <  2:1371271/140‑1 (MQ=255)

                                                                   aTTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTAACCGTGTTAGACAGggt                                                                    <  2:469732/140‑1 (MQ=255)

                                                                     tAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTAACCGTGTTAGACAGggtgg                                                                  <  1:1030740/140‑1 (MQ=255)

                                                                            tcatAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTAACCGTGTTAGACAGGGTGGTAAAGaa                                                           >  1:414779/1‑140 (MQ=255)

                                                                            tcatAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTAACCGTGTTAGACAGGGTGGTAAAGaa                                                           >  2:264013/1‑140 (MQ=255)

                                                                             catAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTAACCGTGTTAGACAGGGTGGTAAAGaaa                                                          >  1:580976/1‑140 (MQ=255)

                                                                                 aTCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTAACCGTGTTAGACAGGGTGGTAAAGAAATTCt                                                      >  1:847547/1‑140 (MQ=255)

                                                                                 aTCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTAACCGTGTTAGACAGGGTGGTAAAGAAATTCt                                                      >  2:1069916/1‑140 (MQ=255)

                                                                                    aaCGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTAACCGTGTTAGACAGGGTGGTAAAGAAATTCTGca                                                   <  1:517231/140‑1 (MQ=255)

                                                                                     aCGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTAACCGTGTTAGACAGGGTGGTAAAGAAATTCTGcac                                                  <  1:1069916/140‑1 (MQ=255)

                                                                                          gAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTAACCGTGTTAGACAGGGTGGTAAAGAAATTCTGCACAGAtt                                             <  2:726990/140‑1 (MQ=255)

                                                                                           aaTCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTAACCGTGTTAGACAGGGTGGTAAAGAAATTCTGCACAGAttt                                            >  1:210304/1‑140 (MQ=255)

                                                                                           aaTCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTAACCGTGTTAGACAGGGTGGTAAAGAAATTCTGCACAGAttt                                            >  1:1032750/1‑140 (MQ=255)

                                                                                                ggTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTAACCGTGTTAGACAGGGTGGTAAAGAAATTCTGCACAGATTTTAACt                                       >  1:1250480/1‑140 (MQ=255)

                                                                                                ggTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTAACCGTGTTAGACAGGGTGGTAAAGAAATTCTGCACAGATTTTAACt                                       <  1:993497/140‑1 (MQ=255)

                                                                                                ggTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTAACCGTGTTAGACAGGGTGGTAAAGAAATTCTGCACAGATTTTAACt                                       >  1:1384179/1‑140 (MQ=255)

                                                                                                 gttgttTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTAACCGTGTTAGACAGGGTGGTAAAGAAATTCTGCACAGATTTTAACtt                                      >  1:1162776/1‑140 (MQ=255)

                                                                                                     tttCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTAACCGTGTTAGACAGGGTGGTAAAGAAATTCTGCACAGATTTTAACTTGttc                                  >  1:1267317/1‑140 (MQ=255)

                                                                                                     tttCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTAACCGTGTTAGACAGGGTGGTAAAGAAATTCTGCACAGATTTTAACTTGttc                                  >  1:52146/1‑140 (MQ=255)

                                                                                                       tCAGTTTCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTAACCGTGTTAGACAGGGTGGTAAAGAAATTCTGCACAGATTTTAACTTGttctt                                <  1:264013/140‑1 (MQ=255)

                                                                                                           tttCCGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTAACCGTGTTAGACAGGGTGGTAAAGAAATTCTGCACAGATTTTAACTTGTTCTTCAAt                            >  2:1306516/1‑140 (MQ=255)

                                                                                                               cGTTTTTATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTAACCGTGTTAGACAGGGTGGTAAAGAAATTCTGCACAGATTTTAACTTGTTCTTCAATTCTg                        >  2:118643/1‑140 (MQ=255)

                                                                                                                   tttATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTAACCGTGTTAGACAGGGTGGTAAAGAAATTCTGCACAGATTTTAACTTGTTCTTCAATTCTGGAAt                    <  2:1389459/140‑1 (MQ=255)

                                                                                                                     tATCTCACCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTAACCGTGTTAGACAGGGTGGTAAAGAAATTCTGCACAGATTTTAACTTGTTCTTCAATTCTGGAATc                   >  2:429334/1‑139 (MQ=255)

                                                                                                                         tcACCGATGTCGGCGGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTAACCGTGTTAGACAGGGTGGTAAAGAAATTCTGCACAGATTTTAACTTGTTCTTCAATTCTGGAATCAGttt              <  1:1068666/140‑1 (MQ=255)

                                                                                                                           aCCGATGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTAACCGTGTTAGACAGGGTGGTAAAGAAATTCTGCACAGATTTTAACTTGTTCTTCAATTCTGGAATCAGTTTTc            >  2:476439/1‑140 (MQ=255)

                                                                                                                               aTGGCGGCTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTAACCGTGTTAGACAGGGTGGTAAAGAAATTCTGCACAGATTTTAACTTGTTCTTCAATTCTGGAATCAGTTTTCCTTc        >  2:1014328/1‑140 (MQ=255)

                                                                                                                                     gcTGTTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTAACCGTGTTAGACAGGGTGGTAAAGAAATTCTGCACAGATTTTAACTTGTTCTTCAATTCTGGAATCAGTTTTCCTTCAACTAc  >  2:703730/1‑140 (MQ=255)

GTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCGCTTGTTTAACCGTGTTAGACAGGGTGGTAAAGAAATTCTGCACAGATTTTAACTTGTTCTTCAATTCTGGAATCAGTTTTCCTTCAACTAC  >  NC_000913/1229519‑1229791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`