Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,560,986	A→C	intergenic (+88/‑382)	yffL → / → yffM	CPZ‑55 prophage; uncharacterized protein YffL/CPZ‑55 prophage; uncharacterized protein YffM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,560,986	0	A	C	100.0%	310.5 / NA	83	intergenic (+88/‑382)	yffL/yffM	CPZ‑55 prophage; uncharacterized protein YffL/CPZ‑55 prophage; uncharacterized protein YffM
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (40/43);  total (40/43)

AGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGCCTAGAG  >  NC_000913/2560852‑2561121                                                                                                                                       |                                                                                                                                        aGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGt                                                                                                                                    <  1:1030976/140‑1 (MQ=255) aGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGt                                                                                                                                    <  1:963359/140‑1 (MQ=255) aGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGt                                                                                                                                    <  1:515986/140‑1 (MQ=255)  gTGCAACGAATACCAGTTATTATTCAAGCCGTACAACTTGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGtt                                                                                                                                   >  1:561281/1‑140 (MQ=255)  gTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGtt                                                                                                                                   >  2:1373886/1‑140 (MQ=255)   tgaaccGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGCCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTg                                                                                                                                  <  1:851868/135‑1 (MQ=255)     cAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGcc                                                                                                                                >  2:323697/1‑140 (MQ=255)      aaCGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAG‑TGCCGTTAGTGGTGGGTCCAAAGt                                                                                                                                    >  2:498139/1‑134 (MQ=255)      aaCGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAG‑TGCCGTTAGTGGTGGGTCCAAAGt                                                                                                                                    <  1:498139/134‑1 (MQ=255)          aaTACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTaa                                                                                                                           >  1:788634/1‑140 (MQ=255)                   tattatTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCa                                                                                                                  >  1:1210564/1‑140 (MQ=255)                    attattCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTATTGGTGGGTCCAAAGTTGCCGTTAAGGAGTGTCaa                                                                                                                 >  2:313059/1‑140 (MQ=255)                    attattCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCaa                                                                                                                 >  1:963163/1‑140 (MQ=255)                    attattCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCaa                                                                                                                 >  1:1367943/1‑140 (MQ=255)                       attCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAg                                                                                                              >  1:543801/1‑140 (MQ=255)                         tCAAGCCGTACAACTTGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGtt                                                                                                            <  2:561281/140‑1 (MQ=255)                         tCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGtt                                                                                                            >  1:1383649/1‑140 (MQ=255)                         tCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGtt                                                                                                            <  1:472011/140‑1 (MQ=255)                         tCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGtt                                                                                                            <  2:602358/140‑1 (MQ=255)                          cAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTg                                                                                                           >  1:1053107/1‑140 (MQ=255)                            aGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGt                                                                                                             <  2:1404373/136‑1 (MQ=255)                            aGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGt                                                                                                             >  1:1404373/1‑136 (MQ=255)                            aGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGcc                                                                                                         >  2:208970/1‑140 (MQ=255)                            aGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGcc                                                                                                         >  2:952188/1‑140 (MQ=255)                             gCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTCGCCGTTAAGGTGGGTCAAAAGTTGCCg                                                                                                        >  2:364263/1‑140 (MQ=255)                                 tACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTaa                                                                                                    >  2:1031039/1‑140 (MQ=255)                                  acaacaTGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAg                                                                                                   >  1:565302/1‑140 (MQ=255)                                  acaacaTGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAg                                                                                                   >  2:513337/1‑140 (MQ=255)                                   caacaTGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAgg                                                                                                  <  2:1154867/140‑1 (MQ=255)                                        tGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTgggg                                                                                             >  2:351256/1‑139 (MQ=255)                                           aaTAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCaa                                                                                          >  2:1368183/1‑140 (MQ=255)                                           aaTAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCaa                                                                                          >  2:10937/1‑140 (MQ=255)                                             tAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTTGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCaaaa                                                                                        >  1:745061/1‑140 (MQ=255)                                              aaCGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAg                                                                                       >  1:1046585/1‑140 (MQ=255)                                                cGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGtt                                                                                     >  1:216116/1‑140 (MQ=255)                                                cGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGtt                                                                                     >  2:1185667/1‑140 (MQ=255)                                                  gAATCAGGCGGGCAATGTTCCAGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGc                                                                                   <  2:1383649/140‑1 (MQ=255)                                                     tCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGa                                                                                >  1:1261672/1‑140 (MQ=255)                                                       aGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGt                                                                              <  2:1046585/140‑1 (MQ=255)                                                          cGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAg                                                                           <  2:963163/140‑1 (MQ=255)                                                            ggCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGaa                                                                         <  2:1053107/140‑1 (MQ=255)                                                                 tGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTctg                                                                    <  2:1210564/140‑2 (MQ=255)                                                                        gCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTTGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTaa                                                             <  2:745061/140‑1 (MQ=255)                                                                        gCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTaa                                                             <  1:1185667/140‑1 (MQ=255)                                                                             ttCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAAtt                                                        >  1:819346/1‑140 (MQ=255)                                                                             ttCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGCACAAAGCTGCCGTTCAGGTGGGTCAAAAGTTGCCGTTAAGGGACGTCAAAAGTTGCCGAGTAAGGATTCTACTGTTAAGaaat                                                        >  2:904139/1‑138 (MQ=37)                                                                                    ccTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCg                                                 >  2:247300/1‑140 (MQ=255)                                                                                         aTTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACggg                                            <  1:208970/140‑1 (MQ=255)                                                                                          ttCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGa                                           <  1:1031039/140‑1 (MQ=255)                                                                                              aTGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGttt                                       >  2:1056278/1‑140 (MQ=255)                                                                                              aTGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGttt                                       >  1:1350177/1‑140 (MQ=255)                                                                                                   ttCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGGCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAg                                  >  2:609091/1‑140 (MQ=255)                                                                                                      aGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCt                               >  2:66578/1‑140 (MQ=255)                                                                                                        gTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTcc                             >  2:202839/1‑140 (MQ=255)                                                                                                          gggTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCcg                           <  2:788634/140‑1 (MQ=255)                                                                                                          gggTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCcg                           <  2:154120/140‑1 (MQ=255)                                                                                                           ggTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCcgg                          >  2:1020945/1‑140 (MQ=255)                                                                                                           ggTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCAcgg                          >  1:1406687/1‑140 (MQ=255)                                                                                                            gTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCcggt                         <  2:1381324/140‑1 (MQ=255)                                                                                                            gTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCcggt                         <  2:651469/140‑1 (MQ=255)                                                                                                            gTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCcggt                         <  1:85123/140‑1 (MQ=255)                                                                                                            gTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCcggt                         <  1:13994/140‑1 (MQ=255)                                                                                                            gTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCcggt                         <  1:247300/140‑1 (MQ=255)                                                                                                            gTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCcggt                         <  2:268417/140‑1 (MQ=255)                                                                                                            gTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCcggt                         <  2:1367943/140‑1 (MQ=255)                                                                                                            gTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCcggt                         >  2:85123/1‑140 (MQ=255)                                                                                                            gTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCcggt                         <  2:171748/140‑1 (MQ=255)                                                                                                            gTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCcggt                         <  2:1420406/140‑1 (MQ=255)                                                                                                            gTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCcggt                         <  2:1406687/140‑1 (MQ=255)                                                                                                            gTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCcggt                         >  1:439543/1‑140 (MQ=255)                                                                                                            gTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCcggt                         <  1:463371/140‑1 (MQ=255)                                                                                                            gTCAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCcggt                         <  1:513337/140‑1 (MQ=255)                                                                                                              cAAAAGTTGCCGTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCcggtcg                       <  1:66578/140‑1 (MQ=255)                                                                                                                         gTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATagagag            <  2:1261672/140‑1 (MQ=255)                                                                                                                         gTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATagagag            <  2:819346/140‑1 (MQ=255)                                                                                                                         gTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATagagag            <  1:1056278/140‑1 (MQ=255)                                                                                                                         gTTAGTGGTGGGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTAATagagag            <  1:952188/140‑1 (MQ=255)                                                                                                                              tggtggGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGcc       <  1:323697/140‑1 (MQ=255)                                                                                                                              tggtggGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGcc       <  1:116849/140‑1 (MQ=255)                                                                                                                              tggtggGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGcc       <  1:1368183/140‑1 (MQ=255)                                                                                                                              tggtggGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGcc       <  2:216116/140‑1 (MQ=255)                                                                                                                               ggtggGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGCCt      >  1:616491/1‑140 (MQ=255)                                                                                                                                gtggGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGCCTa     <  2:616491/140‑1 (MQ=255)                                                                                                                                 tggGTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGCCTag    >  1:53146/1‑140 (MQ=255)                                                                                                                                   ggTCCAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGCCTagag  >  2:1029596/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        AGTGCAACGAATACCAGTTATTATTCAAGCCGTACAACATGAAATAACGGAATCAGGCGGGCAATGTTCCCGCCTTTTCTTTGCCTTAATTCCATGAATTCAGGTGGGTCAAAAGTTGCCGTTAGTGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGTTAAGGTGGGTCAAAAGTTGCCGAGTAAGAATTCTACTATTAAGAATTCTAGGCGACGGGAGTTTTTTAGCCTCCCGGTCGGTCATAGAGAGTCGCCTAGAG  >  NC_000913/2560852‑2561121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑