| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,234,465 | C→A | intergenic (‑10/+274) | higB ← / ← rlmG | ribosome‑dependent mRNA interferase toxin HigB/23S rRNA m(2)G1835 methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,234,465 | 0 | C | A | 98.9% | 316.9 / ‑3.9 | 89 | intergenic (‑10/+274) | higB/rlmG | ribosome‑dependent mRNA interferase toxin HigB/23S rRNA m(2)G1835 methyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (35/53); total (35/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
GCTTTTAATGACTCAGGTTTTTTGAAATATCCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTG > NC_000913/3234328‑3234600 | gCTTTTAATGACTCAGGTTTTTTGAAATATCCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGt > 1:1177996/1‑140 (MQ=255) ttttAATGACTCAGGTTTTTTGAAATATCCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTcc > 1:953437/1‑140 (MQ=255) tAATGACTCAGGTTTTTTGAAATATCCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGtt < 1:512272/140‑1 (MQ=255) tAATGACTCAGGTTTTTTGAAATATCCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGtt < 2:1111983/140‑1 (MQ=255) aaTGACTCAGGTTTTTTGAAATATCCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTg > 2:969789/1‑140 (MQ=255) attaCTCAGGTTTTTTGAAATATCCCTTAGCAATCGTGTCCCCCAGAGCCTCCAACTCGGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGAATTCTTCATCACGTCCGTTGa < 2:233204/137‑1 (MQ=255) gACTCAGGTTTTTTGAAATATCCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACa > 2:157002/1‑140 (MQ=255) tCAGGTTTTTTGAAATATCCCTTAGCAATCGTGTTCCCCATAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAAt > 1:921326/1‑140 (MQ=255) cAGGTTTTTTGAAATATCCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAAtt < 2:918833/140‑1 (MQ=255) ggTTTTTTGAAATATCCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTgg < 1:1113414/140‑1 (MQ=255) ggTTTTTTGAAATATCCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTgg < 1:598856/140‑1 (MQ=255) ggTTTTTTGAAATATCCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTgg < 1:1035962/140‑1 (MQ=255) gTTTTTTGAAATATCCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGc < 2:512021/140‑1 (MQ=255) gTTTTTTGAAATATCCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGc < 1:991777/140‑1 (MQ=255) gTTTTTTGAAATATCCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGc < 2:1273283/140‑1 (MQ=255) gTTTTTTGAAATATCCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGc < 1:777223/140‑1 (MQ=255) gTTTTTTGAAATATCCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGc < 1:671493/140‑1 (MQ=255) ttttttGAAATATCCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCa < 2:848541/140‑1 (MQ=255) ttttttGAAATATCCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCa < 1:337629/140‑1 (MQ=255) ttttGAAATATCCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAat < 1:461704/140‑1 (MQ=255) ttttGAAATATCCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAat > 2:892130/1‑140 (MQ=255) ttttGAAATATCCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAat < 2:1343459/140‑1 (MQ=255) tttGAAATATCCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAata < 1:665314/140‑1 (MQ=255) ttGAAATATCCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAatat < 2:1291819/140‑1 (MQ=255) ttGAAATATCCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAatat > 1:1258048/1‑140 (MQ=255) tGAAATATCCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATg > 1:1324642/1‑140 (MQ=255) aaaTATCCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGAt < 1:892130/140‑1 (MQ=255) atatCCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATaa < 2:420393/140‑1 (MQ=255) cccTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATc < 2:953437/140‑1 (MQ=255) cccTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATc > 2:448821/1‑140 (MQ=255) cccTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATc < 1:448821/140‑1 (MQ=255) aGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGtt > 2:813158/1‑139 (MQ=255) aGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGtt < 1:813158/139‑1 (MQ=255) gCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGc < 1:157002/140‑1 (MQ=255) gCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGc < 1:69770/140‑1 (MQ=255) aTCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAg > 2:925015/1‑140 (MQ=255) tCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGc > 1:828012/1‑136 (MQ=255) tCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGc < 2:828012/136‑1 (MQ=255) tCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGa > 2:628822/1‑140 (MQ=255) tCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGa > 2:46776/1‑140 (MQ=255) tCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGa > 1:735941/1‑140 (MQ=255) cGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGAt > 2:1073056/1‑140 (MQ=255) ttCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGc < 2:1002365/140‑1 (MQ=255) tCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCa < 1:925015/140‑1 (MQ=255) tCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCa < 2:99677/140‑1 (MQ=255) cAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACgg > 2:343290/1‑140 (MQ=255) cAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACgg < 1:46776/140‑1 (MQ=255) agCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCaa > 2:656287/1‑134 (MQ=255) agCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCaa < 1:656287/134‑1 (MQ=255) agCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATg > 1:213235/1‑140 (MQ=255) accaACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAAtt > 1:204401/1‑140 (MQ=255) ccaACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAAttt > 2:916979/1‑140 (MQ=255) aaCTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTAt < 1:969789/140‑1 (MQ=255) aaCTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTAt < 1:1355628/140‑1 (MQ=255) aaCTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTAt < 1:916979/140‑1 (MQ=255) cGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCtgtg < 2:833253/140‑1 (MQ=255) tttATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGa < 1:912691/140‑1 (MQ=255) tATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACg > 1:776154/1‑140 (MQ=255) tGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGga > 2:1154969/1‑140 (MQ=255) gCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGgaagaa > 1:33615/1‑140 (MQ=255) cGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCGGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAAt < 2:1258048/140‑1 (MQ=255) cGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAAt > 1:490057/1‑140 (MQ=255) cGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAAt < 2:33615/140‑1 (MQ=255) tCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGaa < 1:841813/140‑1 (MQ=255) tCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGaa < 1:1007514/140‑1 (MQ=255) ccGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGaaa < 1:673860/140‑1 (MQ=255) agcaTCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACg < 1:1073056/140‑1 (MQ=255) cTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTaaa < 2:735941/140‑1 (MQ=255) cTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGCTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTaaa > 2:543041/1‑140 (MQ=255) tCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACaa < 2:921326/140‑1 (MQ=255) cAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAag < 2:213235/140‑1 (MQ=255) cAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAag < 1:343290/140‑1 (MQ=255) aaTGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAaga < 2:7170/140‑1 (MQ=255) tGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAaga > 1:730895/1‑138 (MQ=255) tGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAaga < 2:730895/138‑1 (MQ=255) tGCTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAgaga < 2:776154/140‑1 (MQ=255) cTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACg > 1:790082/1‑126 (MQ=255) cTTTTTGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGATGCCAGATTGGCAACGGATGAATTTATCTGTGCGACGGAAGAATAAACGTAGAAAAACg < 2:790082/126‑1 (MQ=255) ttttGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGt > 1:910830/1‑140 (MQ=255) ttttGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGCTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGt < 1:543041/140‑1 (MQ=255) ttGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAg < 2:1177996/140‑1 (MQ=255) ttGAGTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAg < 2:490057/140‑1 (MQ=255) gTTATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGa < 2:1324642/140‑1 (MQ=255) ttATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGAt < 1:1265126/140‑1 (MQ=255) tATCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATg > 2:663342/1‑140 (MQ=255) tCAGGTGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGt > 1:1270686/1‑140 (MQ=255) tGCATTCTTCATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAg > 2:346663/1‑140 (MQ=255) tcttcATCAAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGAtt > 1:1271540/1‑140 (MQ=255) catcaAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGAtttttt > 2:1356294/1‑140 (MQ=255) atcaAGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTg > 2:494910/1‑140 (MQ=255) | GCTTTTAATGACTCAGGTTTTTTGAAATATCCCTTAGCAATCGTGTTCCCCAGAGCCACCAACTCCGTTTTATGTTGCGGGTATTTTTCCGCAGCATCTTTCAATGCTTTTTGAGTTATCAGGTGCATTCTTCATCACGTCCGTTGACAAATTGGCAATATGATAACATCCGTTGCCAGATTGGCAACGGATGAATTTATCTGTGTGACGGAAGAATAAACGTAGAAAAACGCTAAACAAGAGAATGTAGACGATGGTGACAGGGATTTTTTG > NC_000913/3234328‑3234600 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |