Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,918,912	C→A	E316* (GAA→TAA)	atpA ←	ATP synthase F1 complex subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,918,912	0	C	A	100.0%	268.3 / NA	77	E316* (GAA→TAA)	atpA	ATP synthase F1 complex subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (37/40);  total (37/40)

TTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGAC  >  NC_000913/3918776‑3919046                                                                                                                                         |                                                                                                                                       ttGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAAcc                                                                                                                                     >  2:335614/1‑140 (MQ=255)     aCAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTg                                                                                                                                 <  2:1152612/140‑1 (MQ=255)        ggttggttTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTg                                                                                                                              <  2:1139668/140‑1 (MQ=255)        ggttggttTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTg                                                                                                                              <  1:438448/140‑1 (MQ=255)         gttggttTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGa                                                                                                                             <  1:762894/140‑1 (MQ=255)         gttggttTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGa                                                                                                                             <  1:538772/140‑1 (MQ=255)         gttggttTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCAATTAACCTTTGGTGa                                                                                                                             >  2:412892/1‑140 (MQ=255)           tggttTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAg                                                                                                                           >  2:97486/1‑140 (MQ=255)                tCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTc                                                                                                                      <  1:145158/140‑1 (MQ=255)                 ccAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACACAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCa                                                                                                                     >  2:992374/1‑140 (MQ=255)                   aGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAAc                                                                                                                   >  2:1241814/1‑140 (MQ=255)                     gAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGt                                                                                                                 >  2:49915/1‑140 (MQ=255)                     gAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTACCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGt                                                                                                                 >  1:377647/1‑140 (MQ=255)                        gaccTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCCCCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTAtt                                                                                                              <  1:1431229/137‑1 (MQ=255)                        gATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTAtt                                                                                                              <  1:49915/140‑1 (MQ=255)                        gATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTAtt                                                                                                              <  1:201223/140‑1 (MQ=255)                               ccATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGtt                                                                                                       <  2:836051/140‑1 (MQ=255)                                  tCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAac                                                                                                    >  2:244211/1‑140 (MQ=255)                                   cGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAaca                                                                                                   >  2:1191422/1‑140 (MQ=255)                                   cGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAaca                                                                                                   >  1:202061/1‑140 (MQ=255)                                   cGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAaca                                                                                                   >  1:546601/1‑140 (MQ=255)                                    ggTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTAtt                                                                                                              >  2:1110797/1‑128 (MQ=255)                                    ggTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTAtt                                                                                                              <  1:1110797/128‑1 (MQ=255)                                       aaTGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTg                                                                                               <  1:735611/140‑1 (MQ=255)                                         tGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTgca                                                                                             >  1:328434/1‑140 (MQ=255)                                          ggAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTgcag                                                                                            <  1:1114815/140‑1 (MQ=255)                                          ggAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTgcag                                                                                            <  2:1103584/140‑1 (MQ=255)                                            agaTTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTgcagca                                                                                          <  1:244211/140‑1 (MQ=255)                                            agaTTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTgcagca                                                                                          <  1:1241814/140‑1 (MQ=255)                                            agaTTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTgcagca                                                                                          <  2:995018/140‑1 (MQ=255)                                              aTTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGtt                                                                                                       <  2:112398/125‑1 (MQ=255)                                              aTTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGtt                                                                                                       >  1:112398/1‑125 (MQ=255)                                              aTTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACg                                                                                        >  2:1143822/1‑140 (MQ=255)                                              aTTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACg                                                                                        >  2:678213/1‑140 (MQ=255)                                              aTTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACg                                                                                        >  2:847673/1‑140 (MQ=255)                                               ttACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGc                                                                                       >  2:353379/1‑140 (MQ=255)                                               ttACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGc                                                                                       >  1:548480/1‑140 (MQ=255)                                                tACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCt                                                                                      >  2:1363625/1‑140 (MQ=255)                                                tACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCt                                                                                      >  1:1089683/1‑140 (MQ=255)                                                tACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCt                                                                                      >  1:1263535/1‑140 (MQ=255)                                                 gcGTTGGTCGGAACGACCGCAGAAACGTCCCCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTc                                                                                     <  2:328434/139‑1 (MQ=255)                                                  cGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTcc                                                                                    >  2:1158932/1‑140 (MQ=255)                                                      ggTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCcagca                                                                                >  1:1307451/1‑140 (MQ=255)                                                       gTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGTACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCcagcag                                                                               >  1:1320701/1‑140 (MQ=255)                                                       gTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCcagcag                                                                               >  2:1016809/1‑140 (MQ=255)                                                       gTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCcagcag                                                                               >  2:519560/1‑140 (MQ=255)                                                        tCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGa                                                                              <  1:1158932/140‑1 (MQ=255)                                                         cGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGAc                                                                             <  1:1191422/140‑1 (MQ=255)                                                         cGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGAc                                                                             <  1:458792/140‑1 (MQ=255)                                                          ggAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACg                                                                            >  1:1410383/1‑140 (MQ=255)                                                            aacgaacgCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACgag                                                                          >  2:1401148/1‑140 (MQ=255)                                                              cgaacgCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGagag                                                                        <  2:1022000/140‑1 (MQ=255)                                                              cgaacgCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGagag                                                                        <  1:847673/140‑1 (MQ=255)                                                              cgaacgCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGagag                                                                        <  2:791823/140‑1 (MQ=255)                                                                      gAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAATGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGagag                                                                        <  1:1396679/132‑1 (MQ=255)                                                                      gAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAATGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGagag                                                                        >  2:1396679/1‑132 (MQ=255)                                                                             cACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACg                                                         <  1:863606/140‑1 (MQ=255)                                                                                 cGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGc                                                     <  1:815471/140‑1 (MQ=255)                                                                                   ccTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGccc                                                   >  1:1253048/1‑140 (MQ=255)                                                                                     tGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCgg                                                 >  1:284550/1‑140 (MQ=255)                                                                                       aGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGa                                               >  2:608556/1‑140 (MQ=255)                                                                                        gTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGaa                                              <  2:546601/140‑1 (MQ=255)                                                                                        gTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGaa                                              <  2:459263/140‑1 (MQ=255)                                                                                            cGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCt                                          <  1:1016809/140‑1 (MQ=255)                                                                                                 aTCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACg                                     >  2:210047/1‑140 (MQ=255)                                                                                                  tCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGt                                    >  1:287546/1‑140 (MQ=255)                                                                                                  tCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGt                                    >  2:732271/1‑140 (MQ=255)                                                                                                   cGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTc                                   >  1:490311/1‑140 (MQ=255)                                                                                                           cGGTCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGga                           >  2:802958/1‑140 (MQ=255)                                                                                                              tCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGgacga                        <  1:447118/140‑1 (MQ=255)                                                                                                              tCAGAGAACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGgacga                        <  1:519560/140‑1 (MQ=255)                                                                                                                   gaACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGag                   <  2:23754/140‑1 (MQ=255)                                                                                                                   gaACCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGag                   <  1:961974/140‑1 (MQ=255)                                                                                                                     aCCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGagca                 <  1:1401148/140‑1 (MQ=255)                                                                                                                     aCCGGTTTTCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGagca                 <  2:729843/140‑1 (MQ=255)                                                                                                                          ttttCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAgg            <  1:97486/140‑1 (MQ=255)                                                                                                                           tttCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAggg           <  1:493246/140‑1 (MQ=255)                                                                                                                           tttCCCTTTCACTTAACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAggg           <  1:882799/140‑1 (MQ=255)                                                                                                                                   tcacttaacCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGa   <  1:802958/140‑1 (MQ=255)                                                                                                                                    cacttaacCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGAc  >  2:569523/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       TTGAACAGGTTGGTTTCCAGGAAGATCTGACCATCGGTAATGGAGATTACGTTGGTCGGAACGAACGCAGAAACGTCACCCGCCTGAGTTTCGATAATCGGCAGTGCGGTCAGAGAACCGGTTTTCCCTTTCACTTCACCTTTGGTGAAGGCTTCAACGTATTCGGCGTTAACACGTGCAGCACGCTCCAGCAGACGAGAGTGGAGGTAGAAAACGTCGCCCGGGAATGCTTCACGTCCTGGCGGACGACGGAGCAGCAGGGAGATCTGAC  >  NC_000913/3918776‑3919046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑