BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,261,710	C→A	S14R (AGC→AGA)	dusA →	tRNA‑dihydrouridine synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,261,710	0	C	A	100.0%	551.5 / NA	154	S14R (AGC→AGA)	dusA	tRNA‑dihydrouridine synthase A
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (86/68); total (86/68)

Only 100 of 155 total aligned reads displayed.

TTATGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGCGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGAAATGGT  >  NC_000913/4261574‑4261844

ttATGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCa                                                                                                                                      <  1:1050118/140‑1 (MQ=255)

  aTGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAAt                                                                                                                                    >  1:345812/1‑140 (MQ=255)

   tGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATg                                                                                                                                   >  1:1180453/1‑140 (MQ=255)

        taGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGa                                                                                                                              >  1:87065/1‑140 (MQ=255)

         aGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGaa                                                                                                                             >  2:1088657/1‑140 (MQ=255)

          gTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGaaa                                                                                                                            >  1:1047667/1‑140 (MQ=255)

          gTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGaaa                                                                                                                            >  1:489205/1‑140 (MQ=255)

           tACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGaaaa                                                                                                                           >  1:1355854/1‑140 (MQ=255)

             ccccGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGc                                                                                                                                  >  2:996394/1‑131 (MQ=255)

             ccccGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGc                                                                                                                                  <  1:996394/131‑1 (MQ=255)

              cccGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAAc                                                                                                                        >  1:463913/1‑140 (MQ=255)

              cccGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAAc                                                                                                                        >  2:456598/1‑140 (MQ=255)

               ccGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACt                                                                                                                       >  1:1082540/1‑140 (MQ=255)

                cGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTg                                                                                                                      >  2:111682/1‑140 (MQ=255)

                cGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTg                                                                                                                      >  2:423706/1‑140 (MQ=255)

                cGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTg                                                                                                                      >  1:781619/1‑140 (MQ=255)

                  cTATTGTGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGAc                                                                                                                    <  1:386743/140‑1 (MQ=255)

                  cTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGAc                                                                                                                    >  1:603239/1‑140 (MQ=255)

                                 aaTAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGt                                                                                                     <  2:1162280/140‑1 (MQ=255)

                                 aaTAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGt                                                                                                     <  1:278483/140‑1 (MQ=255)

                                 aaTAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGt                                                                                                     <  1:423706/140‑1 (MQ=255)

                                 aaTAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGt                                                                                                     <  2:620192/140‑1 (MQ=255)

                                      tGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGtt                                                                                               >  2:1057957/1‑140 (MQ=255)

                                        aTGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCa                                                                                                               >  2:1077672/1‑123 (MQ=255)

                                        aTGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGtt                                                                                               >  2:1019189/1‑138 (MQ=255)

                                        aTGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGtt                                                                                               <  1:1019189/138‑1 (MQ=255)

                                        aTGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGAACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTa                                                                                             >  2:676399/1‑140 (MQ=255)

                                             gAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGtt                                                                                        <  2:526966/140‑1 (MQ=255)

                                                   cataacCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCg                                                                                          <  2:888627/132‑1 (MQ=255)

                                                   cataacCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCg                                                                                          >  1:888627/1‑132 (MQ=255)

                                                        ccccATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCt                                                                             >  1:1060298/1‑140 (MQ=255)

                                                        ccccATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCt                                                                             >  2:730211/1‑140 (MQ=255)

                                                          ccATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCg                                                                           >  1:735430/1‑140 (MQ=255)

                                                             tGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACt                                                                        >  2:156739/1‑140 (MQ=255)

                                                             tGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACt                                                                        >  2:161324/1‑140 (MQ=255)

                                                             tGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACt                                                                        >  1:999226/1‑140 (MQ=255)

                                                             tGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACt                                                                        >  2:699264/1‑140 (MQ=255)

                                                               atatatCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTgg                                                                      <  2:1225443/140‑1 (MQ=255)

                                                                   atCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTGACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTggacgg                                                                  >  1:950048/1‑140 (MQ=255)

                                                                   atCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTggacgg                                                                  >  2:1146981/1‑140 (MQ=255)

                                                                   atCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTggacgg                                                                  >  1:814483/1‑140 (MQ=255)

                                                                   atCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTggacgg                                                                  >  1:234872/1‑140 (MQ=255)

                                                                   atCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTggacgg                                                                  >  2:578958/1‑140 (MQ=255)

                                                                   atCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTggacgg                                                                  >  1:700041/1‑140 (MQ=255)

                                                                   atCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTggacgg                                                                  >  1:1243396/1‑140 (MQ=255)

                                                                   atCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTgga                                                                     <  2:397252/137‑1 (MQ=255)

                                                                     cGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTggacggac                                                                >  1:971508/1‑140 (MQ=255)

                                                                      gATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGgaca                                                               >  2:370066/1‑140 (MQ=255)

                                                                       aTAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGgacag                                                              >  1:1111375/1‑140 (MQ=255)

                                                                       aTAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGgacag                                                              >  1:879595/1‑140 (MQ=255)

                                                                            ataatCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACAtt                                                         <  1:382266/140‑1 (MQ=255)

                                                                             taatCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTg                                                        <  1:1088657/140‑1 (MQ=255)

                                                                               atCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGcc                                                      >  2:965198/1‑140 (MQ=255)

                                                                                 ctctACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGc                                                    <  1:43136/140‑1 (MQ=255)

                                                                                  tctACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCt                                                   >  1:752903/1‑140 (MQ=255)

                                                                                   ctACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTa                                                  <  1:573813/140‑1 (MQ=255)

                                                                                       aTTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTc                                              >  2:732659/1‑140 (MQ=255)

                                                                                        tttGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCt                                             <  2:1047667/140‑1 (MQ=255)

                                                                                        tttGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCt                                             <  2:1120152/140‑1 (MQ=255)

                                                                                         ttGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCtt                                            >  1:1410261/1‑140 (MQ=255)

                                                                                            aaaaTGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCg                                         >  2:813428/1‑140 (MQ=255)

                                                                                              aaTGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTc                                       >  2:78143/1‑140 (MQ=255)

                                                                                              aaTGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTc                                       <  2:1390631/140‑1 (MQ=255)

                                                                                              aaTGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTc                                       >  1:328630/1‑140 (MQ=255)

                                                                                                tGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTg                                     >  1:1365547/1‑140 (MQ=255)

                                                                                                tGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTg                                     <  2:234872/140‑1 (MQ=255)

                                                                                                  cACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCt                                   <  1:965198/140‑1 (MQ=255)

                                                                                                  cACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCt                                   >  2:320628/1‑140 (MQ=255)

                                                                                                     ggTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTc                                <  2:1365547/140‑1 (MQ=255)

                                                                                                      gTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTcc                               >  2:367168/1‑140 (MQ=255)

                                                                                                          ttCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCa                           >  1:438263/1‑140 (MQ=255)

                                                                                                          ttCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCa                           <  2:700041/140‑1 (MQ=255)

                                                                                                             tGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATa                        <  2:120037/140‑1 (MQ=255)

                                                                                                             tGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATa                        <  2:1402585/140‑1 (MQ=255)

                                                                                                             tGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATa                        <  2:87065/140‑1 (MQ=255)

                                                                                                             tGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATa                        <  2:463913/140‑1 (MQ=255)

                                                                                                             tGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATa                        <  2:752903/140‑1 (MQ=255)

                                                                                                              gAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATa                        <  1:693849/140‑1 (MQ=255)

                                                                                                               aaaTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACg                      <  1:1185635/140‑1 (MQ=255)

                                                                                                               aaaTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACg                      <  1:933108/140‑1 (MQ=255)

                                                                                                               aaaTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACg                      <  2:735430/140‑1 (MQ=255)

                                                                                                                 aTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAATCTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATa                        <  1:207679/136‑1 (MQ=255)

                                                                                                                 aTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCGCCAATGCTCGACTGGACGGACAGACATTGCCGCTAGTTCTTGCGTCTGCTTGCCCGCAATACGtt                    <  2:670735/140‑1 (MQ=255)

                                                                                                                 aTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACg                      <  1:1137673/138‑1 (MQ=255)

                                                                                                                 aTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGtt                    >  2:536769/1‑140 (MQ=255)

                                                                                                                 aTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGtt                    >  1:1006146/1‑140 (MQ=255)

                                                                                                                 aTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGAC‑GGTTTAGCGTTGCATCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATa                        >  2:207679/1‑136 (MQ=255)

                                                                                                                  tGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTg                   <  1:111682/140‑1 (MQ=255)

                                                                                                                  tGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTg                   <  2:915741/140‑1 (MQ=255)

                                                                                                                  tGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTg                   <  2:48310/140‑1 (MQ=255)

                                                                                                                    cAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACg                      >  2:1247123/1‑135 (MQ=255)

                                                                                                                    cAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCt                 <  1:730211/140‑1 (MQ=255)

                                                                                                                    cAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCt                 <  2:1180453/140‑1 (MQ=255)

                                                                                                                    cAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCt                 <  1:1229755/140‑1 (MQ=255)

                                                                                                                    cAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCt                 <  2:1111375/140‑1 (MQ=255)

                                                                                                                     aaaaaaTCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTg                >  1:682289/1‑140 (MQ=255)

                                                                                                                       aaaaTCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGta              <  1:1077631/140‑1 (MQ=255)

                                                                                                                                   accaGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGAAATGGt  <  1:156739/140‑1 (MQ=255)

                                                                                                                                   accaGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGAAATGGt  <  2:108233/140‑1 (MQ=255)

                                                                                                                                   accaGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGAAATGGt  >  2:946863/1‑140 (MQ=255)

TTATGGTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGCGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTC‑GGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGAAATGGT  >  NC_000913/4261574‑4261844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`