Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	747,037	G→T	L245I (CTT→ATT)	abrB ←	putative regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	747,037	0	G	T	100.0%	142.0 / NA	41	L245I (CTT→ATT)	abrB	putative regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (18/23);  total (18/23)

TAAAATCAATGTGCATAAACCGGGTCAGCCCCCACGCCATACCCGCACAAATAGCCAGCAGAGCAAAAATCGACAGCAGGATTTGCGGTAGCGGGCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAAGACCAATCCGCCAGCCAATTGCCATATACGCCATCGCCAGCAGCCATTCCGGTAGTTCGATGGTGATGAGCTGACCAGACTGGAGCACTGCGCCCGCCAGCATCGGGATGAGCATCGTGCCGGAAGGAAGAC  >  NC_000913/746905‑747168                                                                                                                                     |                                                                                                                                    tAAAATCAATGTGCATAAACCGGGTCAGCCCCCACGCCATACCCGCACAAATAGCCAGCAGAGCAAAAATCGACAGCAGGATTTGCGGTAGCGGGCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAATACCAATc                                                                                                                              >  2:640290/1‑140 (MQ=255)    aaTCAATGTGCATAAACCGGGTCAGCCCCCACGCCATACCCGCACAAATAGCCAGCAGAGCAAAAATCGACAGCAGGATTTGCGGTAGCGGGCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAATACCAATCCgc                                                                                                                           <  2:1193867/140‑1 (MQ=255)        aaTGTGCATAAACCGGGTCAGCCCCCACGCCATACCCGCACAAATAGCCAGCAGAGCAAAAATCGACAGCAGGATTTGCGGTAGCGGGCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAATACCAATCCgccagc                                                                                                                       <  1:822625/140‑1 (MQ=255)        aaTGTGCATAAACCGGGTCAGCCCCCACGCCATACCCGCACAAATAGCCAGCAGAGCAAAAATCGACAGCAGGATTTGCGGTAGCGGGCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAATACCAATCCgccagc                                                                                                                       <  1:138036/140‑1 (MQ=255)         aTGTGCATAAACCGGGTCAGCCCCCACGCCATACCCGCACAAATAGCCAGCAGAGCAAAAATCGACAGCAGGATTTGCGGTAGCGGGCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAATACCAATCCgccagcc                                                                                                                      <  1:1232725/140‑1 (MQ=255)         aTGTGCATAAACCGGGTCAGCCCCCACGCCATACCCGCACAAATAGCCAGCAGAGCAAAAATCGACAGCAGGATTTGCGGTAGCGGGCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAATACCAATCCgccagcc                                                                                                                      <  1:1030687/140‑1 (MQ=255)          tgtgCATAAACCGGGTCAGCCCCCACGCCATACCCGCACAAATAGCCAGCAGAGCAAAAATCGACAGCAGGATTTGCGGTAGCGGGCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAATACCAATCCgccagcca                                                                                                                     >  1:685170/1‑140 (MQ=255)           gtgCATAAACCGGGTCAGCCCCCACGCCATACCCGCACAAATAGCCAGCAGAGCAAAAATCGACAGCAGGATTTGCGGTAGCGGGCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAATACCAATCCGCCAGCCaa                                                                                                                    <  1:1048893/140‑1 (MQ=255)           gtgCATAAACCGGGTCAGCCCCCACGCCATACCCGCACAAATAGCCAGCAGAGCAAAAATCGACAGCAGGATTTGCGGTAGCGGGCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAATACCAATCCGCCAGCCaa                                                                                                                    <  2:730251/140‑1 (MQ=255)           gtgCATAAACCGGGTCAGCCCCCACGCCATACCCGCACAAATAGCCAGCAGAGCAAAAATCGACAGCAGGATTTGCGGTAGCGGGCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAATACCAATCCGCCAGCCaa                                                                                                                    <  2:1254908/140‑1 (MQ=255)            tgCATAAACCGGGTCAGCCCCCACGCCATACCCGCACAAATAGCCAGCAGAGCAAAAATCGACAGCAGGATTTGCGGTAGCGGGCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAATACCAATCCGCCAGCCAAt                                                                                                                   <  1:87009/140‑1 (MQ=255)            tgCATAAACCGGGTCAGCCCCCACGCCATACCCGCACAAATAGCCAGCAGAGCAAAAATCGACAGCAGGATTTGCGGTAGCGGGCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAATACCAATCCGCCAGCCAAt                                                                                                                   >  1:671887/1‑140 (MQ=255)              cATAAACCGGGTCAGCCCCCACGCCATACCCGCACAAATAGCCAGCAGAGCAAAAATCGACAGCAGGATTTGCGGTAGCGGGCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAATACCAATCCGCCAGCCAATTg                                                                                                                 <  1:640290/140‑1 (MQ=255)              cATAAACCGGGTCAGCCCCCACGCCATACCCGCACAAATAGCCAGCAGAGCAAAAATCGACAGCAGGATTTGCGGTAGCGGGCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAATACCAATCCGCCAGCCAATTg                                                                                                                 <  2:442375/140‑1 (MQ=255)                tAAACCGGGTCAGCCCCCACGCCATACCCGCACAAATAGCCAGCAGAGCAAAAATCGACAGCAGGATTTGCGGTAGCGGGCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAATACCAATCCGCCAGCCAATTGcc                                                                                                               <  1:1244187/140‑1 (MQ=255)                      gggTCAGCCCCCACGCCATACCCGCACAAATAGCCAGCAGAGCAAAAATCGACAGCAGGATTTGCGGTAGCGGGCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAATACCAATCCGCCAGCCAATTGCCATATAc                                                                                                         >  2:1240477/1‑140 (MQ=255)                       ggTCAGCCCCCACGCCATACCCGCACAAATAGCCAGCAGAGCAAAAATCGACAGCAGGATTTGCGGTAGCGGGCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAATACCAATCCGCCAGCCAATTGCCATATACg                                                                                                        <  2:996/140‑1 (MQ=255)                        gTCAGCCCCCACGCCATACCCGCACAAATAGCCAGCAGAGCAAAAATCGACAGCAGGATTTGCGGTAGCGGGCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAATACCAATCCGCCAGCCAATTGCCATATACGc                                                                                                       >  1:108071/1‑140 (MQ=255)                                        tACCCGCACAAATAGCCAGCAGAGCAAAAATCGACAGCAGGATTTGCGGTAGCGGGCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAATACCAATCCGCCAGCCAATTGCCATATACGCCATCGCcagca                                                                                            <  2:707242/135‑1 (MQ=255)                                        tACCCGCACAAATAGCCAGCAGAGCAAAAATCGACAGCAGGATTTGCGGTAGCGGGCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAATACCAATCCGCCAGCCAATTGCCATATACGCCATCGCcagca                                                                                            >  1:707242/1‑135 (MQ=255)                                              cacaAATAGCCAGCAGAGCAAAAATCGACAGCAGGATTTGCGGTAGCGGGCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAATACCAATCCGCCAGCCAATTGCCATATACGCCATCGCCAGCAGCCATTCCGGt                                                                                 >  2:538157/1‑140 (MQ=255)                                              cacaAATAGCCAGCAGAGCAAAAATCGACAGCAGGATTTGCGGTAGCGGGCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAATACCAATCCGCCAGCCAATTGCCATATACGCCATCGCCAGCAGCCATTCCGGt                                                                                 >  1:1171975/1‑140 (MQ=255)                                                caAATAGCCAGCAGAGCAAAAATCGACAGCAGGATTTGCGGTAGCGGGCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAATACCAATCCGCCAGCCAATTGCCATATACGCCATCGCCAGCAGCCATTCCGGTAg                                                                               <  1:940527/140‑1 (MQ=255)                                                            agagCAAAAATCGACAGCAGGATTTGCGGTAGCGGGCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAATACCAATCCGCCAGCCAATTGCCATATACGCCATCGCCAGCAGCCATTCCGGTAGTTCGATGGtgat                                                                   <  1:1155353/140‑1 (MQ=255)                                                                cAAAAATCGACAGCAGGATTTGCGGTAGCGGGCGTAGTGCCCGCAGTAAGATTTGTTTATCTAAACCAATACCAATCCGCCAGCCAATTGCCATATACGCCATCGCCAGCAGCCATTTCGGTAGTTCGATGGTGATGAGc                                                               >  1:1400703/1‑140 (MQ=255)                                                                 aaaaaTCGACAGCAGGATTTGCGGTAGCGGGCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAATACCAATCCGCCAGCCAATTGCCATATACGCCATCGCCAGCAGCCATTCCGGTAGTTCGATGGTGATGAGCt                                                              >  2:1376533/1‑140 (MQ=255)                                                                    aaTCGACAGCAGGATTTGCGGTAGCGGGCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAATACCAATCCGCCAGCCAATTGCCATATACGCCATCGCCAGCAGCCATTCCGGTAGTTCGATGGTGATGAGCTGAc                                                           <  1:1376533/140‑1 (MQ=255)                                                                    aaTCGACAGCAGGATTTGCGGTAGCGGGCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAATACCAATCCGCCAGCCAATTGCCATATACGCCATCGCCAGCAGCCATTCCGGTAGTTCGATGGTGATGAGCTGAc                                                           <  2:685170/140‑1 (MQ=255)                                                                    aaTCGACAGCAGGATTTGCGGTAGCGGGCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAATACCAATCCGCCAGCCAATTGCCATATACGCCATCGCCAGCAGCCATTCCGGTAGTTCGATGGTGATGAGCTGAc                                                           <  2:671887/140‑1 (MQ=255)                                                                          cagcagGATTTGCGGTAGCGGGCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAATACCAATCCGCCAGCCAATTGCCATATACGCCATCGCCAGCAGCCATTTCGGTAGTTCGATGGTGATGAGCTGACCAGACt                                                     <  2:1400703/140‑1 (MQ=255)                                                                             cagGATTTGCGGTAGCGGGCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAATACCAATCCGCCAGCCAATTGCCATATACGCCATCGCCAGCAGCCATTCCGGTAGTTCGATGGTGATGAGCTGACCAGACTGGa                                                  >  2:744409/1‑140 (MQ=255)                                                                                       ggTAGCGGGCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAATACCAATCCGCCAGCCAATTGCCATATACGCCATCGCCAGCAGCCATTCCGGTAGTTCGATGGTGATGAGCTGACCAGACTGGAGCACTGcgcc                                        >  2:6509/1‑140 (MQ=255)                                                                                              ggCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAATACCAATCCGCCAGCCAATTGCCATATACGCCATCGCCAGCAGCCATTCCGGTAGTTCGATGGTGATGAGCTGACCAGACTGGAGCACTGCGCCCGCCAGc                                 >  1:1261561/1‑140 (MQ=255)                                                                                               gCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAATACCAATCCGCCAGCCAATTGCCATATACGCCATCGCCAGCAGCCATTCCGGTAGTTCGATGGTGATGAGCTGACCAGACTGGAGCACTGCGCCCGCCAGCa                                <  1:1240477/140‑1 (MQ=255)                                                                                                cGTGGTGCCCGCAGAAAGTTTTTTTTATCAAAACAAATCCCAACCCCCCAGCCAATTGCCATATACGCCATCGCCAGCAGCCATTCCGGTAGTTCGATGGGGATGAGCTGACCAGACTGGAGCACTGCGCCCGCCAGCAt                               <  2:1321322/140‑1 (MQ=255)                                                                                                 gTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAATACCAACCCGCCAGCCAATTGCCATATACGCCATCGCCAGCAGCCATTCCGGTAGTTCGATGGTGATGAGCTGACCAGACTGGAGCACTGCGCCCGCCAGCATc                              <  2:762783/140‑1 (MQ=255)                                                                                                      gCCCGCAGTAAGATTTGTTTATCGAAACCAATACCAACCCGCCAGCCAATTGCCATATACGCCATCGCCAGCAGCCATTCCGGTAGTTCGATGGTGATGAGCTGACCAGACTGGAGCACTGCGCCCGCCAGCATCGGGAt                         <  1:1293063/140‑1 (MQ=255)                                                                                                          gCAGTAAGATTTGTTTATCGAAACCAATACCAATCCGCCAGCCAATTGCCATATACGCCATCGCCAGCAGCCATTCCGGTAGTTCGATGGTGATGAGCTGACCAGACTGGAGCACTGCGCCCGCCAGCATCGGGATGAGc                     >  1:1321322/1‑140 (MQ=255)                                                                                                                      gTTTATCGAAACCAATACCAATCCGCCAGCCAATTGCCATATACGCCATCGCCAGCAGCCATTCCGGTAGTTCGATGGTGATGAGCTGACCAGACTGGAGCACTGCGCCCGCCAGCATCGGGATGAGCATCGTGCCggaa         >  1:606071/1‑140 (MQ=255)                                                                                                                      gTTTATCGAAACCAATACCAATCCGCCAGCCAATTGCCATATACGCCATCGCCAGCAGCCATTCCGGTAGTTCGATGGTGATGAGCTGACCAGACTGGAGCACTGCGCCCGCCAGCATCGGGATGAGCATCGTGCCggaa         >  2:822129/1‑140 (MQ=255)                                                                                                                      gTTTATCGAAACCAATACCAATCCGCCAGCCAATTGCCATATACGCCATCGCCAGCAGCCATTCCGGTAGTTCGATGGTGATGAGCTGACCAGACTGGAGCACTGCGCCCGCCAGCATCGGGATGAGCATCGTGCCggaa         >  2:980072/1‑140 (MQ=255)                                                                                                                        ttATCGAAACCAATACCAATCCGCCAGCCAATTGCCATATACGCCATCGCCAGCAGCCATTCCGGTAGTTCGATGGTGATGAGCTGACCAGACTGGAGCACTGCGCCCGCCAGCATCGGGATGAGCATCGTGCCggaagg       >  2:711779/1‑140 (MQ=255)                                                                                                                             gAACCCAATACCAATCCGCCAGCCAATTGCCATATACGCCATCGCCAGCAGCCATTCCGGTAGTTCGATGGTGATGAGCTGACCAGACTGGAGCACTGCGCCCGCCAGCATCGGGATGAGCATCGTGCCGGAAGGAAGAc  <  1:1091724/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                    TAAAATCAATGTGCATAAACCGGGTCAGCCCCCACGCCATACCCGCACAAATAGCCAGCAGAGCAAAAATCGACAGCAGGATTTGCGGTAGCGGGCGTAGTGCCCGCAGTAAGATTTGTTTATCGAAACCAAGACCAATCCGCCAGCCAATTGCCATATACGCCATCGCCAGCAGCCATTCCGGTAGTTCGATGGTGATGAGCTGACCAGACTGGAGCACTGCGCCCGCCAGCATCGGGATGAGCATCGTGCCGGAAGGAAGAC  >  NC_000913/746905‑747168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑