Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	102,587	C→A	R119S (CGC→AGC)	ddlB →	D‑alanine‑‑D‑alanine ligase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	102,587	0	C	A	100.0%	141.5 / NA	40	R119S (CGC→AGC)	ddlB	D‑alanine‑‑D‑alanine ligase B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (23/17);  total (23/17)

GGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCATCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCCGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGTTAAGCCGAGCCGCGAAGGTTCCAGTGTGGGAATGTCAAAAGTAGTAGCAGAAAATGCTCTACA  >  NC_000913/102451‑102723                                                                                                                                         |                                                                                                                                         ggggATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCATCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCagcg                                                                                                                                       >  2:537856/1‑140 (MQ=255) ggggATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCATCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCagcg                                                                                                                                       <  1:160921/140‑1 (MQ=255) ggggATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCATCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCagcg                                                                                                                                       <  1:537856/140‑1 (MQ=255)  gggATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCATCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCAgcgc                                                                                                                                      <  1:155099/140‑1 (MQ=255)         tCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCATCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCagcg                                                                                                                                       >  1:130434/1‑132 (MQ=255)         tCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCATCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCagcg                                                                                                                                       <  2:130434/132‑1 (MQ=255)         tCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCATCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCAGCGCAGAGttt                                                                                                                               >  2:212128/1‑140 (MQ=255)                 aTGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCATCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCAGCGCAGAGTTTGAAAAAgg                                                                                                                       >  1:498637/1‑140 (MQ=255)                  tGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCATCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCAGCGCAGAGTTTGAAAAAGGc                                                                                                                      >  1:279290/1‑140 (MQ=255)                    ggCTTGCCTTATACCGGAAGCGGAGTGATGGCATCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCAGCGCAGAGTTTGAAAAAGGCCt                                                                                                                    <  1:47493/140‑1 (MQ=255)                               taCCGGAAGCGGAGTGATGGCATCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCAGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGc                                                                                                         >  1:458047/1‑140 (MQ=255)                                    gAAGCGGAGTGATGGCATCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCAGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGCTGTTa                                                                                                    >  1:80976/1‑140 (MQ=255)                                    gAAGCGGAGTGATGGCATCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCAGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTa                                                                                                    <  2:279290/140‑1 (MQ=255)                                    gAAGCGGAGTGATGGCATCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCAGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTa                                                                                                    >  2:108419/1‑140 (MQ=255)                                     aaGCGGAGTGATGGCATCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCAGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTAg                                                                                                   >  2:667263/1‑140 (MQ=255)                                                  gCATCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCAGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTAGCAGAAATTTCTGc                                                                                      >  2:409402/1‑140 (MQ=255)                                                     tCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCAGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTAGCAGAAATTTCTGctct                                                                                   >  1:506059/1‑140 (MQ=255)                                                       tGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCAGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTgg                                                                                 >  1:306222/1‑140 (MQ=255)                                                                        aaCTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCAGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGtt                                                                >  1:635336/1‑140 (MQ=255)                                                                               cagcaAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCAGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGTTAagccga                                                         >  1:485150/1‑140 (MQ=255)                                                                                 gcaAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCAGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGTTAagccgagc                                                       <  1:132191/140‑1 (MQ=255)                                                                                  caAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCAGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGTTAagccgagcc                                                      >  2:420636/1‑140 (MQ=255)                                                                                  caAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCAGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGTTAagccgagcc                                                      >  1:115637/1‑140 (MQ=255)                                                                                  caAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCAGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGTTAagccgagcc                                                      >  1:392099/1‑140 (MQ=255)                                                                                  caAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCAGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGTTAagccgagcc                                                      >  1:645065/1‑140 (MQ=255)                                                                                  caAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCAGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGTTAagccgagcc                                                      >  1:58947/1‑140 (MQ=255)                                                                                    aaCTTCTATGGCAAGGTGCCGGTTTGCCGGTCGCGCCGGTGGTAGCTGTAACCAGGGTAGAGTTTGAAAAAGGCTTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTATGGGTTTGCCGTTTATCGTTAAGCCGAGCcgc                                                    <  2:567967/140‑1 (MQ=255)                                                                                     aCTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCAGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGTTAAGCCGAGCcgcg                                                   <  1:409402/140‑1 (MQ=255)                                                                                      cTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCAGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGTTAAGCCGAGCCGCGa                                                  <  2:292646/140‑1 (MQ=255)                                                                                      cTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCAGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGTTAAGCCGAGCCGCGa                                                  <  1:212128/140‑1 (MQ=255)                                                                                       ttCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCAGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGTTAAGCCGAGCCGCGaa                                                 >  2:420205/1‑140 (MQ=255)                                                                                            tGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCAGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGTTAAGCCGAGCCGCGAAGGTTc                                            <  2:485150/140‑1 (MQ=255)                                                                                              gCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCAGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGTTAAGCCGAGCCGCGAAGGTTCCa                                          <  1:108133/140‑1 (MQ=255)                                                                                              gCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCAGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGTTAAGCCGAGCCGCGAAGGTTCCa                                          <  2:324697/140‑1 (MQ=255)                                                                                              gCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCAGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGTTAAGCCGAGCCGCGAAGGTTCCa                                          <  1:667263/140‑1 (MQ=255)                                                                                                 aGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCAGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGTTAAGCCGAGCCGCGAAGGTTCCAgtg                                       >  1:442534/1‑140 (MQ=255)                                                                                                 aGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCAGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGTTAAGCCGAGCCGCGAAGGTTCCAgtg                                       >  2:89727/1‑140 (MQ=255)                                                                                                 aGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCAGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGTTAAGCCGAGCCGCGAAGGTTCCAgtg                                       >  2:184171/1‑140 (MQ=255)                                                                                                 aGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCAGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGTTAAGCCGAGCCGCGAAGGTTCCAgtg                                       >  2:709169/1‑140 (MQ=255)                                                                                                      ccGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCAGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGCTGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGTTAAGCCGAGCCGCGAAGGTTCCAGTGTGGGa                                  <  2:80976/140‑1 (MQ=255)                                                                                                      ccGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCAGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGTTAAGCCGAGCCGCGAAGGTTCCAGTGTGGGa                                  <  2:99988/140‑1 (MQ=255)                                                                                                      ccGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCAGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGTTAAGCCGAGCCGCGAAGGTTCCAGTGTGGGa                                  <  1:89727/140‑1 (MQ=255)                                                                                                      ccGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCAGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGTTAAGCCGAGCCGCGAAGGTTCCAGTGTGGGa                                  >  1:673392/1‑140 (MQ=255)                                                                                                            tACCGGTCGCGCCGTGGGTAGCGTTAACCAGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGTTAAGCCGAGCCGCGAAGGTTCCAGTGTGGGAATGTCa                            <  2:673392/140‑1 (MQ=255)                                                                                                                          tGGGTAGCGTTAACCAGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGTTAAGCCGAGCCGCGAAGGTTCCAGTGTGGGAATGTCAAAAGTAGTAGCAGa              <  2:306222/140‑1 (MQ=255)                                                                                                                           gggTAGCGTTAACCAGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGTTAAGCCGAGCCGCGAAGGTTCCAGTGTGGGAATGTCAAAAGTAGTAGCAGaa             <  1:709169/140‑1 (MQ=255)                                                                                                                                      aCCAGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGTTAAGCCGAGCCGCGAAGGTTCCAGTGTGGGAATGTCAAAAGTAGTAGCAGAAAATGCTCTACa  >  1:508461/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         GGGGATGCTCGAGCTGATGGGCTTGCCTTATACCGGAAGCGGAGTGATGGCATCTGCGCTTTCAATGGATAAACTACGCAGCAAACTTCTATGGCAAGGTGCCGGTTTACCGGTCGCGCCGTGGGTAGCGTTAACCCGCGCAGAGTTTGAAAAAGGCCTGAGCGATAAGCAGTTAGCAGAAATTTCTGCTCTGGGTTTGCCGGTTATCGTTAAGCCGAGCCGCGAAGGTTCCAGTGTGGGAATGTCAAAAGTAGTAGCAGAAAATGCTCTACA  >  NC_000913/102451‑102723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑