Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,349,168	A→G	S314P (TCT→CCT)	yciW ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,349,168	0	A	G	100.0%	170.5 / NA	46	S314P (TCT→CCT)	yciW	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/22);  total (24/22)

CGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGAATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGATTTTTTTATATTCATTTGATGAATCCATACAGGTGGCAAAACAAGCAGGACTGCCATTTATACGCGCGCATAA  >  NC_000913/1349033‑1349297                                                                                                                                        |                                                                                                                                  cGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGGATGa                                                                                                                               >  2:278042/1‑140 (MQ=255)     ccATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGGATGACGGt                                                                                                                           <  1:542895/140‑1 (MQ=255)              cagATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGGATGACGGTCAGCCCAtt                                                                                                                  >  2:18026/1‑140 (MQ=255)                   taataaTCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGGATGACGGt                                                                                                                           <  1:298829/126‑1 (MQ=255)                   taataaTCTGCGCTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGGATGACGGt                                                                                                                           >  2:298829/1‑126 (MQ=255)                          cTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCAt                                                                                                      >  1:138776/1‑140 (MQ=255)                           tGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCAtt                                                                                                     >  2:145483/1‑140 (MQ=255)                             cGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGGATGACGGTCAGCCCa                                                                                                                    >  1:101411/1‑123 (MQ=255)                             cGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGGATGACGGTCAGCCCa                                                                                                                    <  2:101411/123‑1 (MQ=255)                             cGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCAtttt                                                                                                   >  2:383538/1‑140 (MQ=255)                               ttGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCATTTTCg                                                                                                 >  2:565249/1‑140 (MQ=255)                                 gatgatTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCATTTTCGcc                                                                                               >  1:323182/1‑140 (MQ=255)                                  atgatTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCATTTTCGccc                                                                                              <  1:383538/140‑1 (MQ=255)                                   tgatTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCt                                                                                             >  2:388541/1‑140 (MQ=255)                                    gatTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCtt                                                                                            <  2:323182/140‑1 (MQ=255)                                            gtgtTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATaa                                                                                    <  2:86849/140‑1 (MQ=255)                                                            cTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGAttttt                                                                      <  1:375112/138‑1 (MQ=255)                                                            cTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGAttttt                                                                      >  2:375112/1‑138 (MQ=255)                                                              gAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGATTTTTTtat                                                                  >  2:607793/1‑140 (MQ=255)                                                              gAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGATTTTTTtat                                                                  >  2:355061/1‑140 (MQ=255)                                                               aaCTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGATTTTTTtata                                                                 <  1:11579/140‑1 (MQ=255)                                                                    ggcggcGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGAttttt                                                                      >  1:675328/1‑130 (MQ=255)                                                                    ggcggcGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGAttttt                                                                      <  2:675328/130‑1 (MQ=255)                                                                    ggcggcGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGATTTTTTTATATTCAt                                                            >  1:29026/1‑140 (MQ=255)                                                                    ggcggcGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGATTTTTTTATATTCAt                                                            >  2:165092/1‑140 (MQ=255)                                                                    ggcggcGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGATTTTTTTATATTCAt                                                            >  1:256477/1‑140 (MQ=255)                                                                    ggcggcGCTAAAGCGATCCGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGATCAACAGGATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGAGCATAAGGGTGCTGATTTTTTTATATTCAt                                                            >  2:701091/1‑140 (MQ=255)                                                                      cggcgCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGATTTTTTTATATTCAttt                                                          >  2:347727/1‑140 (MQ=255)                                                                        gcgcTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCTCTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGATTTTTTTATATTCATTtga                                                        <  1:409318/140‑1 (MQ=255)                                                                        gcgcTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGATTTTTTTATATTCATTtga                                                        <  1:607793/140‑1 (MQ=255)                                                                        gcgcTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGATTTTTTTATATTCATTtga                                                        <  1:18026/140‑1 (MQ=255)                                                                        gcgcTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGATTTTTTTATATTCATTtga                                                        <  2:705654/140‑1 (MQ=255)                                                                          gcTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGATTTTTTTATATTCATTtgatg                                                      <  2:29026/140‑1 (MQ=255)                                                                           cTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGATTTTTTTATATTCATTtgatga                                                     <  1:565249/140‑1 (MQ=255)                                                                                gCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGATTTTTTTATATTCATTTGATGAATCCa                                                <  1:388541/140‑1 (MQ=255)                                                                                 cGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGATTTTTTTATATTCATTTGATGAATCCAt                                               <  1:178925/140‑1 (MQ=255)                                                                                   aTCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGATTTTTTTATATTCATTTGATGAATCCATAc                                             >  1:710969/1‑140 (MQ=255)                                                                                                  cAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGTTGATTTTTTTATAATCATTTGATGAATCCATACAGGTGGCAAAACAAg                              <  1:434674/140‑1 (MQ=255)                                                                                                        ttGTATCGCCTGAACGGTAGCGCGCTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGATTTTTTTATATTCATTTGATGAATCCATACAGGTGGCAAAACAAGCAGGAc                        <  1:145483/140‑1 (MQ=255)                                                                                                         tGTATCGCCTGAACGGTAGCGCGCTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGATTTTTTTATATTCATTTGATGAATCCATACAGGTGGCAAAACAAGCAGGACt                       >  1:95565/1‑140 (MQ=255)                                                                                                          gTATCGCCTGAACGGTAGCGCGCTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGATTTTTTTATATTCATTTGATGAATCCATACAGGTGGCAAAACAAGCAGGACTg                      <  1:278042/140‑1 (MQ=255)                                                                                                                 cTGAACGGTAGCGCGCTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGATTTTTTTATATTCATTTGATGAATCCATACAGGTGGCAAAACAAGCAGGACTGCCATTta               <  2:256477/140‑1 (MQ=255)                                                                                                                   gAACGGTAGCGCGCTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGATTTTTTTATATTCATTTGATGAATCCATACAGGTGGCAAAACAAGCAGGACTGCCATTtata             >  1:70182/1‑140 (MQ=255)                                                                                                                      cGGTAGCGCGCTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGATTTTTTTATATTCATTTGATGAATCCATACAGGTGGCAAAACAAGCAGGACTGCCATTTATAcgc          <  2:95565/140‑1 (MQ=255)                                                                                                                         tAGCGCGCTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGATTTTTTTATATTCATTTGATGAATCCATACAGGTGGCAAAACAAGCAGGACTGCCATTTATAcgcgcg       >  1:135141/1‑140 (MQ=255)                                                                                                                              cgcTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGATTTTTTTATATTCATTTGATGAATCCATACAGGTGGCAAAACAAGCAGGACTGCCATTTATACGCGCGCATaa  >  1:331550/1‑140 (MQ=255)                                                                                                                                cTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGATTTTTTTATATTCATTTGATGAATCCATACAGGTGGCAAAACAAGCAGGACTGCCATTTATAcgcgcg       >  2:204473/1‑133 (MQ=255)                                                                                                                                cTCAACAGGATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGATTTTTTTATATTCATTTGATGAATCCATACAGGTGGCAAAACAAGCAGGACTGCCATTTATAcgcgcg       <  1:204473/133‑1 (MQ=255)                                                                                                                                        |                                                                                                                                  CGCTCCATACCAGCAGATTAATAATCTGCGTTGATGATTTTTCGTGTTCGAGTAAAGGGCTGAACTGGGCGGCGCTAAAGCGATCGGGTGCTCGGGTCAGCCATTGTATCGCCTGAACGGTAGCGCGCTCAACAGAATGACGGTCAGCCCATTGGTTAATTTCATTTTCGCCCTTGCGCATAAGGGTGCTGATTTTTTTATATTCATTTGATGAATCCATACAGGTGGCAAAACAAGCAGGACTGCCATTTATACGCGCGCATAA  >  NC_000913/1349033‑1349297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑