Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,349,854	C→A	R85L (CGC→CTC)	yciW ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,349,854	0	C	A	100.0%	117.1 / NA	34	R85L (CGC→CTC)	yciW	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (16/18);  total (16/18)

GATTTGTGCCAACCGATGATTACTTTCCCTGGTGGAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACACGCGCAGGGTCCAGCCATGAAAGGAAGGGGGCCAGCGTTTCTTTTTCAAGGATCACGTCGAG  >  NC_000913/1349724‑1349987                                                                                                                                   |                                                                                                                                      gATTTGTGCCAACCGATGATTACTTTCCCTGGTGGAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGAGTTCATAAg                                                                                                                              <  1:240254/140‑1 (MQ=255)                 tgatTACTTTCCCTGGTGGAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGAGTTCATAAGCGGTGAAGGt                                                                                                                    <  1:281959/134‑1 (MQ=255)                 tgatTACTTTCCCTGGTGGAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGAGTTCATAAGCGGTGAAGGt                                                                                                                    >  2:281959/1‑134 (MQ=255)                  gatTACTTTCCCTGGTGGAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGAGTTCATAAGCGGTGAAGGTCTGTGaa                                                                                                             <  2:639450/140‑1 (MQ=255)                  gatTACTTTCCCTGGTGGAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGAGTTCATAAGCGGTGAAGGTCTGTGaa                                                                                                             <  2:2970/140‑1 (MQ=255)                      aCTTTCCCTGGTGGAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGAGTTCATAAGCGGTGAAGGTCTGTGAACggg                                                                                                         <  2:581058/140‑1 (MQ=255)                      aCTTTCCCTGGTGGAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGAGTTCATAAGCGGTGAAGGTCTGTGAACggg                                                                                                         <  1:57148/140‑1 (MQ=255)                          tCCCTGGTGGAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGAGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCAc                                                                                                     <  2:226491/140‑1 (MQ=255)                              tggtggAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGAGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTa                                                                                                 >  1:404796/1‑140 (MQ=255)                                gtggAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGAGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTa                                                                                                 <  2:75200/138‑1 (MQ=255)                                gtggAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGAGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTa                                                                                                 >  1:75200/1‑138 (MQ=255)                                        aGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGAGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAg                                                                                       <  2:333510/140‑1 (MQ=255)                                          gCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGAGTTCATAAGCGGTGAAGGTCTGTGAACGGGACACGGTAAGCTGGTCAggg                                                                                     >  2:650226/1‑140 (MQ=255)                                                  aGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGAGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATc                                                                             <  2:7774/140‑1 (MQ=255)                                                        gTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGAGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGc                                                                       <  1:448712/140‑1 (MQ=255)                                                                cgcCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGAGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAacac                                                               >  1:290867/1‑140 (MQ=255)                                                                 gcCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAAAGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACAcg                                                              >  2:196640/1‑140 (MQ=255)                                                                   cGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGAGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACAcgcg                                                            <  2:404796/140‑1 (MQ=255)                                                                               aCATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAGGAGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACACGCGCAGGGTCCAGcc                                                >  2:405353/1‑140 (MQ=255)                                                                               aCATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGAGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACACGCGCAGGGTCCAGcc                                                >  1:588388/1‑140 (MQ=255)                                                                                cATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGAGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACACGCGCAGGGTCCAGCCa                                               <  1:405353/140‑1 (MQ=255)                                                                                    aCCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGAGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGACCACGCGCAGGGTCCa                                                   <  1:351581/132‑1 (MQ=255)                                                                                    aCCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGAGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACACGCGCa                                                          >  2:472325/1‑125 (MQ=255)                                                                                    aCCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGAGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACACGCGCa                                                          <  1:472325/125‑1 (MQ=255)                                                                                    aCCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGAGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACACGCGCAGGGTCCa                                                   >  2:351581/1‑132 (MQ=255)                                                                                    aCCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGAGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACACGCGCAGGGTCCAGCCATGaa                                           >  1:107783/1‑140 (MQ=255)                                                                                        cTGGACGCCGCAAATCTGAGCAACCGTCAGGGCCGTCGACAAGAGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACACGCGCAGGGTCCAGCCATGAaagga                                       >  1:659814/1‑140 (MQ=255)                                                                                        cTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGAGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACACGCGCAGGGTCCAGCCATGAaagga                                       >  1:504222/1‑140 (MQ=255)                                                                                                cgcAAACCTGAGCAACCGTCAGGGCCGTCGACAAGAGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACACGCGCAGGGTCCAGCCATGAAAGGAAGGGGGcc                               >  1:671466/1‑140 (MQ=255)                                                                                                  cAAACCTGAGCAACCGTCAGGGCCGTCGACAAGAGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACACGCGCAGGGTCCAGCCATGAAAGGAAGGGGGCCAg                             >  2:117457/1‑140 (MQ=255)                                                                                                          aGCAACCGTCAGGGCCGTCGACAAGAGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACACGCGCAGGGTCCAGCCATGAAAGGAAGGGGGCCAGCGTTTCtt                     >  2:339663/1‑140 (MQ=255)                                                                                                              aCCGTCAGGGCCGTCGACAAGAGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACACGCGCAGGGTCCAGCCATGAAAGGAAGGGGGCCAGCGTTTCTTTTTc                 <  1:659224/140‑1 (MQ=255)                                                                                                                   cAGGGCCGTCGACAAGAGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACACGCGCAGGGTCCAGCCATGAAAGGAAGGGGGCCAGCGTTTCTTTTTCAAGGa            <  1:112872/140‑1 (MQ=255)                                                                                                                             gACAAGAGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACACGCGCAGGGTCCAGCCATGAAAGGAAGGGGGCCAGCGTTTCTTTTTCAAGGATCACGTCGAg  <  1:117457/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                      GATTTGTGCCAACCGATGATTACTTTCCCTGGTGGAATCAGGCCCGGGGAGCGGCGTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACACGCGCAGGGTCCAGCCATGAAAGGAAGGGGGCCAGCGTTTCTTTTTCAAGGATCACGTCGAG  >  NC_000913/1349724‑1349987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑