Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	121,281	C→A	A91S (GCC→TCC)	aroP ←	aromatic amino acid:H(+) symporter AroP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	121,281	0	C	A	100.0%	184.5 / NA	51	A91S (GCC→TCC)	aroP	aromatic amino acid:H(+) symporter AroP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (18/33);  total (18/33)

CAAAGAATACGGCGGCAGAAACCCAGGTGGGGATTTCCGGATACCAGAACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATA  >  NC_000913/121146‑121408                                                                                                                                        |                                                                                                                                caAAGAATACGGCGGCAGAAACCCAGGTGGGGATTTCCGGATACCAGAACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGAaaaa                                                                                                                             <  1:238265/140‑1 (MQ=255)      aaTACGGCGGCAGAAACCCAGGTGGGGATTTCCGGATACCAGAACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGAAAAACTGcc                                                                                                                        <  1:118657/140‑1 (MQ=255)      aaTACGGCGGCAGAAACCCAGGTGGGGATTTCCGGATACCAGAACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGAAAAACTGcc                                                                                                                        >  2:309577/1‑140 (MQ=255)       aTACGGCGGCAGAAACCCAGGTGGGGATTTCCGGATACCAGAACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGAAAAACTGccc                                                                                                                       <  2:726211/140‑1 (MQ=255)       aTACGGCGGCAGAAACCCAGGTGGGGATTTCCGGATACCAGAACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGAAAAACTGccc                                                                                                                       <  1:267776/140‑1 (MQ=255)       aTACGGCGGCAGAAACCCAGGTGGGGATTTCCGGATACCAGAACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGAAAAACTGccc                                                                                                                       <  2:320877/140‑1 (MQ=255)       aTACGGCGGCAGAAACCCAGGTGGGGATTTCCGGATACCAGAACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGAAAAACTGccc                                                                                                                       <  1:709934/140‑1 (MQ=255)          cggcggCAGAAACCCAGGTGGGGATTTCCGGATACCAGAACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGAAAAACTGCCCCAg                                                                                                                    <  2:46781/140‑1 (MQ=255)          cggcggCAGAAACCCAGGTGGGGATTTCCGGATACCAGAACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGAAAAACTGCCCCAg                                                                                                                    <  1:314244/140‑1 (MQ=255)             cggcAGAAACCCAGGTGGGGATTTCCGGATACCAGAACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGAAAAACTGccc                                                                                                                       <  1:191917/134‑1 (MQ=255)             cggcAGAAACCCAGGTGGGGATTTCCGGATACCAGAACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGAAAAACTGccc                                                                                                                       >  2:191917/1‑134 (MQ=255)               gcAGAAACCCAGGTGGGGATTTCCGGATACCAGAACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGAAAAACTGCCCCAGTAttt                                                                                                               >  1:534451/1‑140 (MQ=255)                      cccAGGTGGGGATTTCCGGATACCAGAACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCa                                                                                                        <  1:222649/140‑1 (MQ=255)                        cAGGTGGGGATTTCCGGATACCAGAACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCaaa                                                                                                      <  2:534451/140‑1 (MQ=255)                        cAGGTGGGGATTTCCGGATACCAGAACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCaaa                                                                                                      <  2:11662/140‑1 (MQ=255)                               ggATTTCCGGATACCAGAACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTa                                                                                               >  1:147707/1‑140 (MQ=255)                                                  cTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGtt                                                                            <  1:426610/140‑1 (MQ=255)                                                     aaTGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGttctt                                                                         >  1:57546/1‑140 (MQ=255)                                                      aTGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGttcttc                                                                        <  1:696230/140‑1 (MQ=255)                                                          aTTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGacc                                                                    >  2:17560/1‑140 (MQ=255)                                                           tttACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGacca                                                                   >  1:474985/1‑140 (MQ=255)                                                           tttACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGacca                                                                   <  2:680623/140‑1 (MQ=255)                                                               cccACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCAt                                                               >  1:58396/1‑140 (MQ=255)                                                                   cGGCAGACAACTCAGCCATGGCAACTACAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGCCAGGTTCTTCGACCACCATTTCa                                                           >  2:453232/1‑140 (MQ=39)                                                                     gCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCAcc                                                         <  1:692627/140‑1 (MQ=255)                                                                              tcagcCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACg                                                >  1:191110/1‑140 (MQ=255)                                                                               cagcCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCGCCCAGCTGACGc                                               <  1:192167/140‑1 (MQ=255)                                                                               cagcCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGc                                               <  2:191110/140‑1 (MQ=255)                                                                               cagcCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGc                                               <  1:378166/140‑1 (MQ=255)                                                                                        cAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACACATGATCAGa                                      <  2:474985/140‑1 (MQ=255)                                                                                           cTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAg                                   <  2:58396/140‑1 (MQ=255)                                                                                                   tACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATaaa                           >  2:517934/1‑140 (MQ=255)                                                                                                        tACCCAGTAGTTCCAGCCAGAGGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAg                      >  1:43655/1‑140 (MQ=255)                                                                                                           ccAGTAGTTCCAGCCAGAGGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCaa                   <  1:517934/140‑1 (MQ=255)                                                                                                             agtagtTCCAGCCAGAGGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATg                 >  1:517348/1‑140 (MQ=255)                                                                                                               tagtTCCAGCCAGAGGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGc               >  1:464875/1‑140 (MQ=255)                                                                                                                agtTCCAGCCAGAGGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCg              >  2:80070/1‑140 (MQ=255)                                                                                                                agtTCCAGCCAGAGGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCg              <  2:523984/140‑1 (MQ=255)                                                                                                                agtTCCAGCCAGAGGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCg              <  2:523887/140‑1 (MQ=255)                                                                                                                agtTCCAGCCAGAGGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCg              >  1:556629/1‑140 (MQ=255)                                                                                                                  ttCCAGCCAGAGGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTa            >  1:333141/1‑140 (MQ=255)                                                                                                                    ccagccagAGGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAAc          >  1:198426/1‑140 (MQ=255)                                                                                                                     cagccagAGGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAAcc         <  2:464875/140‑1 (MQ=255)                                                                                                                     cagccagAGGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAAcc         <  2:517348/140‑1 (MQ=255)                                                                                                                         cagAGGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCg              >  2:401010/1‑131 (MQ=255)                                                                                                                         cagAGGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCg              <  1:401010/131‑1 (MQ=255)                                                                                                                          agagGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGa    <  2:333141/140‑1 (MQ=255)                                                                                                                           gagGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCAAAGTTGCTAAAGGAGCCTGCGACAGTTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt   <  2:43655/140‑1 (MQ=255)                                                                                                                           gagGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt   <  2:147707/140‑1 (MQ=255)                                                                                                                            agGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATa  <  1:309577/140‑1 (MQ=255)                                                                                                                            agGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATa  <  2:556629/140‑1 (MQ=255)                                                                                                                            agGCGAAACCGGAAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATa  <  2:57546/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                CAAAGAATACGGCGGCAGAAACCCAGGTGGGGATTTCCGGATACCAGAACTGAATGTATTTACCCACGGCAGTCAGCTCAGCCATGGCAACTAAAACGTACAGTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATA  >  NC_000913/121146‑121408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑