Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,023,929	T→C	intergenic (+251/‑39)	fliR → / → rcsA	flagellar biosynthesis protein FliR/DNA‑binding transcriptional activator RcsA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,023,929	0	T	C	100.0%	261.9 / NA	69	intergenic (+251/‑39)	fliR/rcsA	flagellar biosynthesis protein FliR/DNA‑binding transcriptional activator RcsA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/41);  total (28/41)

ACATTTACTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGTAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAACCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGAC  >  NC_000913/2023793‑2024063                                                                                                                                         |                                                                                                                                       aCATTTACTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGGAATATTCAGGCAAg                                                                                                                                     >  2:604974/1‑140 (MQ=255) aCATTTACTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAg                                                                                                                                     <  1:658964/140‑1 (MQ=255)   aTTTACTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAggg                                                                                                                                   >  1:499696/1‑140 (MQ=255)     ttactttactTTAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGa                                                                                                                                 >  1:672923/1‑140 (MQ=255)       actttactttAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAAt                                                                                                                               <  1:146927/140‑1 (MQ=255)       actttactttAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAAt                                                                                                                               >  2:678308/1‑140 (MQ=255)       actttactttAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAAt                                                                                                                               >  2:441246/1‑140 (MQ=255)         tttactttaAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTa                                                                                                                             <  1:588491/140‑1 (MQ=255)                taAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTAc                                                                                                                      >  2:266004/1‑140 (MQ=255)                   gACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCa                                                                                                                   <  2:343454/140‑1 (MQ=255)                   gACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCa                                                                                                                   <  1:197512/140‑1 (MQ=255)                        ttCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATtgag                                                                                                              <  2:296961/140‑1 (MQ=255)                         tCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATtgagt                                                                                                             <  2:701681/140‑1 (MQ=255)                         tCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATtgagt                                                                                                             <  1:621901/140‑1 (MQ=255)                         tCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATtgagt                                                                                                             <  1:137567/140‑1 (MQ=255)                         tCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATtgagt                                                                                                             <  1:441246/140‑1 (MQ=255)                         tCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATtgagt                                                                                                             <  2:566419/140‑1 (MQ=255)                         tCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATtgagt                                                                                                             <  2:579434/140‑1 (MQ=255)                          ccAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATtgagtg                                                                                                            <  1:392314/140‑1 (MQ=255)                           cAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATtgagtga                                                                                                           >  1:614397/1‑140 (MQ=255)                             ggCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAgg                                                                                                         <  2:117607/140‑1 (MQ=255)                             ggCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAgg                                                                                                         <  1:41988/140‑1 (MQ=255)                              gCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAggg                                                                                                        >  2:432582/1‑140 (MQ=255)                              gCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAggg                                                                                                        >  1:604377/1‑140 (MQ=255)                               cAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGt                                                                                                       <  2:378016/140‑1 (MQ=255)                                 aaTTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTAt                                                                                                     <  2:8067/140‑1 (MQ=255)                                  aTTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATg                                                                                                    >  2:455714/1‑140 (MQ=255)                                    tataCAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGcc                                                                                                  <  1:266004/140‑1 (MQ=255)                                              tttACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGa                                                                                        >  1:538246/1‑140 (MQ=255)                                                tACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGatt                                                                                      >  2:318723/1‑140 (MQ=255)                                                   gggATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGattatt                                                                                   <  2:604377/140‑1 (MQ=255)                                                    ggATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGAttatta                                                                                  <  1:455714/140‑1 (MQ=255)                                                    ggATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGAttatta                                                                                  <  2:672923/140‑1 (MQ=255)                                                     gATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATtattat                                                                                 >  1:625107/1‑140 (MQ=255)                                                      aTAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATTAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATg                                                                                <  2:148831/140‑1 (MQ=255)                                                        aGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGa                                                                              >  2:67285/1‑140 (MQ=255)                                                         gTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGAt                                                                             <  2:442253/140‑1 (MQ=255)                                                          tAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGAtt                                                                            <  1:318723/140‑1 (MQ=255)                                                            aGTCGGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTa                                                                          >  2:95122/1‑140 (MQ=255)                                                            aGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTa                                                                          >  1:500555/1‑140 (MQ=255)                                                             gTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTAt                                                                         <  1:67704/140‑1 (MQ=255)                                                             gTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTAt                                                                         <  1:623377/140‑1 (MQ=255)                                                                 gCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAg                                                                     >  1:207770/1‑140 (MQ=255)                                                                  ccTGAAGAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGt                                                                    >  2:504075/1‑140 (MQ=255)                                                                  ccTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGt                                                                    >  2:672126/1‑140 (MQ=255)                                                                  ccTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGt                                                                    >  2:612543/1‑140 (MQ=255)                                                                  ccTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGt                                                                    >  1:379568/1‑140 (MQ=255)                                                                  ccTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGt                                                                    <  1:672126/140‑1 (MQ=255)                                                                       aaaaTCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTacac                                                               <  1:322151/140‑1 (MQ=255)                                                                           tCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGTTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGa                                                           <  1:95122/140‑1 (MQ=255)                                                                            cgcgAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGAc                                                          <  1:432582/140‑1 (MQ=255)                                                                             gcgAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACt                                                         <  1:504075/140‑1 (MQ=255)                                                                                      gcgcATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAAc                                                <  1:187226/140‑1 (MQ=255)                                                                                      gcgcATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAAc                                                <  2:528507/140‑1 (MQ=255)                                                                                           ttAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAACCGGGt                                           <  2:433794/140‑1 (MQ=255)                                                                                           ttAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAACCGGGt                                           <  2:143624/140‑1 (MQ=255)                                                                                             aGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAACCGGGTAt                                         <  2:499696/140‑1 (MQ=255)                                                                                                tGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAACCGGGTATCTg                                      <  2:614397/140‑1 (MQ=255)                                                                                                        gttgttCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAACCGGGTATCTGTTGAGTag                              >  1:604333/1‑140 (MQ=255)                                                                                                        gttgttCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAACCGGGTATCTGTTGAGTag                              <  1:583800/140‑1 (MQ=255)                                                                                                          tgttCCGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAACCGGGTATCTGTTGAGTagag                            <  1:67285/140‑1 (MQ=255)                                                                                                               cGTTTAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAACCGGGTATCTGTTGAGTAGAGGGGtt                       >  2:325714/1‑140 (MQ=255)                                                                                                                 tttAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAACCGGGTATCTGTTGAGTAGAGGGGTTaa                     >  1:151515/1‑140 (MQ=255)                                                                                                                   tAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAACCGGGTATCTGTTGAGTAGAGGGGTTaaaa                   >  1:208136/1‑140 (MQ=255)                                                                                                                   tAGTCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAACCGGGTATCTGTTGAGTAGAGGGGTTaaaa                   <  1:604974/140‑1 (MQ=255)                                                                                                                      tCATGATGAAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAACCGGGTATCTGTTGAGTAGAGGGGTTaaaaaaa                >  1:514458/1‑140 (MQ=255)                                                                                                                         tgatgaAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAACCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAagag             <  1:678308/140‑1 (MQ=255)                                                                                                                           atgaAATATTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAACCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGaa           >  1:18867/1‑140 (MQ=255)                                                                                                                                atatTCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAACCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCaa      <  1:443182/140‑1 (MQ=255)                                                                                                                                    tCAGGCAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAACCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGAc  >  2:12515/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       ACATTTACTTTACTTTAAGACAATTCCAGGCAAATTATACAACACTTTACGGGATAGTAAGTCCGCCTGAAAAATCGCGAGAGTGGCGCATTAGGTGACCCATGTTGTTCCGTTTAGTCATGATGAAATATTCAGGTAAGGGGAATTATCGTTACGCATTGAGTGAGGGTATGCCATGTCAACGATTATTATGGATTTATGTAGTTACACCCGACTAGGTTTAACCGGGTATCTGTTGAGTAGAGGGGTTAAAAAAAGAGAAATCAACGAC  >  NC_000913/2023793‑2024063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑