Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,400,815	G→T	T247T (ACC→ACA)	nuoF ←	NADH:quinone oxidoreductase subunit F

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,400,815	0	G	T	100.0%	123.0 / NA	36	T247T (ACC→ACA)	nuoF	NADH:quinone oxidoreductase subunit F
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (18/18);  total (18/18)

CCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTGGTGCCAGCATCTTTACTTTTCGAGATGTTCTGATACCACTCCACGCCGTTAGCGAGGATCGCCGGAACGTTACACAGGGTTTCGACGTTGTTGACACAGGTCGGTTTACCCCATGCGCCGGAGGTTGCCGGG  >  NC_000913/2400678‑2400947                                                                                                                                          |                                                                                                                                     ccTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGTAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTTGt                                                                                                                                    >  2:537485/1‑140 (MQ=255)  cTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTtgtg                                                                                                                                   <  2:562295/140‑1 (MQ=255)                       tCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTTGTGCCAGCATCTTTACTTTTCgag                                                                                                              <  2:474524/140‑1 (MQ=255)                       tCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTTGTGCCAGCATCTTTACTTTTCgag                                                                                                              >  1:482942/1‑140 (MQ=255)                        cAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTTGTGCCAGCATCTTTACTTTTCgaga                                                                                                             <  1:445032/140‑1 (MQ=255)                         aGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGTAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTTGTGCCAGCATCTTTACTTTTCGAGAt                                                                                                            <  1:537485/140‑1 (MQ=255)                         aGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTTGTGCCAGCATCTTTACTTTTCGAGAt                                                                                                            <  1:545828/140‑1 (MQ=255)                             cATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTTGTGCCAGCATCTTTACTTTTCGAGATGTTc                                                                                                        <  1:726264/140‑1 (MQ=255)                             cATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTTGTGCCAGCATCTTTACTTTTCGAGATGTTc                                                                                                        <  1:654600/140‑1 (MQ=255)                                cACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTTGTGCCAGCATCTTTACTTTTCGAGATGt                                                                                                          <  1:2117/135‑1 (MQ=255)                                cACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTTGTGCCAGCATCTTTACTTTTCGAGATGt                                                                                                          >  2:2117/1‑135 (MQ=255)                                cACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTTGTGCCAGCATCTTTACTTTTCGAGATGTTCTGa                                                                                                     <  1:57753/140‑1 (MQ=255)                                             ggCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTTGTGCCAGCATCTTTACTTTTCGAGATGTTCTGATACCACTCCACGc                                                                                        <  1:634631/140‑1 (MQ=255)                                             ggCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTTGTGCCAGCATCTTTACTTTTCGAGATGTTCTGATACCACTCCACGc                                                                                        >  2:327433/1‑140 (MQ=255)                                              gCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTTGTGCCAGCATCTTTACTTTTCGAGATGTTCTGATACCACTCCACGcc                                                                                       >  1:508806/1‑140 (MQ=255)                                               cGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTTGTGCCAGCATCTTTCCTTTTCGAGATGTTCTGATCCCGCCCCACGCCg                                                                                      <  2:389760/140‑1 (MQ=255)                                               cGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTTGTGCCAGCATCTTTACTTTTCGAGATGTTCTGAGACCACTCCACGCCg                                                                                      >  1:688495/1‑140 (MQ=255)                                                   aTCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTTGTGCCAGCATCTTTACTTTTCGAGATGTTCTGATACCACTCCACGCCGTTAg                                                                                  >  2:564629/1‑140 (MQ=255)                                                        cGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTTGTGCCAGCATCTTTACTTTTCGAGATGTTCTGATACCACTCCACGCCGTTAGCGAgg                                                                             <  1:564629/140‑1 (MQ=255)                                                              tctcGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTTGTGCCAGCATCTTTACTTTTCGAGATGTTCTGATACCACTCCACGCCGTTAGCGAGGATCGcc                                                                       >  1:516752/1‑140 (MQ=255)                                                              tctcGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTTGTGCCAGCATCTTTACTTTTCGAGATGTTCTGATACCACTCCACGCCGTTAGCGAGGATCGcc                                                                       >  1:385603/1‑140 (MQ=255)                                                               ctcGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTTGTGCCAGCATCTTTACTTTTCGAGATGTTCTGATACCACTCCACGCCGTTAGCGAGGATCGCCg                                                                      >  2:107334/1‑140 (MQ=255)                                                               ctcGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTTGTGCCAGCATCTTTACTTTTCGAGATGTTCTGATACCACTCCACGCCGTTAGCGAGGATCGCCg                                                                      >  2:567852/1‑140 (MQ=255)                                                               ctcGCGTGCGGTGGTCCGAAACGGCAGTTCCCAAAGCCCCGGCTTTTTCCCCCTACCGGGGAACCCCATCACCTTTGTACCAGCATCTTTTCTTTTCGGGATGTTCTGAGACCCCTCCACGCCGTTAGCGAGGATCGCCg                                                                      <  2:657575/140‑1 (MQ=255)                                                                tcGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTTGTGCCAGCATCTTTACTTTTCGAGATGTTCTGATACCACTCCACGCCGTTAGCGAGGATCGCCgg                                                                     >  2:34862/1‑140 (MQ=255)                                                                 cgcgTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTTGTGCCAGCATCTTTACTTTTCGAGATGTTCTGATACCACTCCACGCCGTCAGCGAGGATCGCCGGa                                                                    >  1:86738/1‑140 (MQ=255)                                                                    gTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTTGTGCCAGCATCTTTACTTTTCGAGATGTTCTGATACCACTCCACGCCGTTAGCGAGGATCGCCGGAACg                                                                 <  2:482942/140‑1 (MQ=255)                                                                    gTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTTGTGCCAGCATCTTTACTTTTCGAGATGTTCTGATACCACTCCACGCCGTTAGCGAGGATCGCCGGAACg                                                                 <  2:516752/140‑1 (MQ=255)                                                                                     ggCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTTGTGCCAGCATCTTTACTTTTCGAGATGTTCTGATACCACTCCACGCCGTTAGCGAGGATCGCCGGAACGTTACACAGGGTTTCGAc                                                <  2:292344/140‑1 (MQ=255)                                                                                     ggCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTTGTGCCAGCATCTTTACTTTTCGAGATGTTCTGATACCACTCCACGCCGTTAGCGAGGATCGCCGGAACGTTACACAGGGTTTCGAc                                                <  2:385603/140‑1 (MQ=255)                                                                                          ttCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTTGTGCCAGCATCTTTACTTTTCGAGATGTTCTGATACCACTCCACGCCGTTAGCGAGGATCGCCGGAACGTTACACAGGGTTTCGACgttgt                                           >  1:550339/1‑140 (MQ=255)                                                                                                   tCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTTGTGCCAGCATCTTTACTTTTCGAGATGTTCTGATACCACTCCACGCCGTTAGCGAGGATCGCCGGAACGTTACACAGGGTTTCGACGTTGTTGACACAgg                                  <  2:508806/140‑1 (MQ=255)                                                                                                   tCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTTGTGCCAGCATCTTTACTTTTCGAGATGTTCTGATACCACTCCACGCCGTTAGCGAGGATCGCCGGAACGTTACACAGGGTTTCGACGTTGTTGACACAgg                                  >  1:673022/1‑140 (MQ=255)                                                                                                   tCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTTGTGCCAGCATCTTTACTTTTCGAGATGTTCTGATACCACTCCACGCCGTTAGCGAGGATCGCCGGAACGTTACACAGGGTTTCGACGTTGTTGACACAgg                                  <  1:327433/140‑1 (MQ=255)                                                                                                   tCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTTGTGCCAGCATCTTTACTTTTCGAGATGTTCTGATACCACTCCACGCCGTTAGCGAGGATCACCGGAACGTTACACAGGGTTTCGACGTTGTTGACACAgg                                  >  1:607098/1‑140 (MQ=255)                                                                                                    cccGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTTGTGCCAGCATCTTTACTTTTCGAGATGTTCTGATACCACTCCACGCCGTTAGCGAGGATCGCCGGAACGTTACACAGGGTTTCGACGTTGTTGACACAGGt                                 >  2:175983/1‑140 (MQ=255)                                                                                                    cccGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTTGTGCCAGCATCTTTACTTTTCGAGATGTTCTGATACCACTCCACGCCGTTAGCGAGGATCGCCGGAACGTTACACAGGGTTTCGACGTTGTTGACACAGGt                                 <  1:175983/140‑1 (MQ=255)                                                                                                                                 cATCAGCTTTGTGCCAGCATCTTTACTTTTCGAGATGTTCTGATACCACTCCACGCCGTTAGCGAGGATCGCCGGAACGTTACACAGGGTTTCGACGTTGTTGACACAGGTCGGTTTACCCCATGCGCCGGAGGTTGCCg    >  2:38800/1‑140 (MQ=255)                                                                                                                                   tCAGCTTTGTGCCAGCATCTTTACTTTTCGAGATGTTCTGATACCACTCCACGCCGTTAGCGAGGATCGCCGGAACGTTACACAGGGTTTCGACGTTGTTGACACAGGTCGGTTTACCCCATGCGCCGGAGGTTGCCggg  <  2:602158/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                     CCTGGCTGCCAGGCTTTAAATTTCAGACCATCACGCATACCACCGGCGTAATCTTCGAGGATCTCGCGTGCGGTGGTGCCGAACGGCAGTTCCCACAGTCCCGGATTTTTCACCCGACCGGAGAAGCCCATCAGCTTGGTGCCAGCATCTTTACTTTTCGAGATGTTCTGATACCACTCCACGCCGTTAGCGAGGATCGCCGGAACGTTACACAGGGTTTCGACGTTGTTGACACAGGTCGGTTTACCCCATGCGCCGGAGGTTGCCGGG  >  NC_000913/2400678‑2400947

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑