Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,478,871	C→A	P342Q (CCG→CAG)	dsdX →	D‑serine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,478,871	0	C	A	100.0%	144.8 / NA	41	P342Q (CCG→CAG)	dsdX	D‑serine transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (21/20);  total (21/20)

ACATACGGAAAATGGCTTCGGTTCTATTGCTAATATTTTGCTGATTATCGGGGCCGGAGGCGCATTCAACGCCATTTTAAAAAGCAGCAGTCTCGCTGATACGCTGGCAGTTATTCTCTCCAATATGCATATGCACCCGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGATGGGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAATT  >  NC_000913/2478734‑2479001                                                                                                                                          |                                                                                                                                   acaTACGGAAAATGGCTTCGGTTCTATTGCTAATATTTTGCTGATTATCGGGGCCGGAGGCGCATTCAACGCCATTTTAAAAAGCAGCAGTCTCGCTGATACGCTGGCAGTTATTCTCTCCAATATGCATATGCACCAGa                                                                                                                                  <  2:492920/140‑1 (MQ=255)     aCGGAAAATGGCTTCGGTTCTATTGCTAATATTTTGCTGATTATCGGGGCCGGAGGCGCATTCAACGCCATTTTAAAAAGCAGCAGTCTCGCTGATACGCTGGCAGTTATTCTCTCCAATATGCATATGCACCAGAttct                                                                                                                              >  2:472894/1‑140 (MQ=255)      cGGAAAATGGCTTCGGTTCTATTGCTAATATTTTGCTGATTATCGGGGCCGGAGGCGCATTCAACGCCATTTTAAAAAGCAGCAGTCTCGCTGATACGCTGGCAGTTATTCTCTCCAATATGCATATGCACCAGAttctt                                                                                                                             <  2:52403/140‑1 (MQ=255)      cGGAAAATGGCTTCGGTTCTATTGCTAATATTTTGCTGATTATCGGGGCCGGAGGCGCATTCAACGCCATTTTAAAAAGCAGCAGTCTCGCTGATACGCTGGCAGTTATTCTCTCCAATATGCATATGCACCAGAttctt                                                                                                                             >  1:382273/1‑140 (MQ=255)      cGGAAAATGGCTTCGGTTCTATTGCTAATATTTTGCTGATTATCGGGGCCGGAGGCGCATTCAACGCCATTTTAAAAAGCAGCAGTCTCGCTGATACGCTGGCAGTTATTCTCTCCAATATGCATATGCACCAGAttctt                                                                                                                             <  2:254943/140‑1 (MQ=255)       ggAAAATGGCTTCGGTTCTATTGCTAATATTTTGCTGATTATCGGGGCCGGAGGCGCATTCAACGCCATTTTAAAAAGCAGCAGTCTCGCTGATACGCTGGCAGTTATTCTCTCCAATATGCATATGCACCAGAttcttc                                                                                                                            >  1:410535/1‑140 (MQ=255)         aaaaTGGCTTCGGTTCTATTGCTAATATTTTGCTGATTATCGGGGCCGGAGGCGCATTCAACGCCATTTTAAAAAGCAGCAGTCTCGCTGATACGCTGGCAGTTATTCTCTCCAATATGCATATGCACCAGATTCTTCTg                                                                                                                          >  1:324664/1‑140 (MQ=255)           aaTGGCTTCGGTTCTATTGCTAATATTTTGCTGATTATCGGGGCCGGAGGCGCATTCAACGCCATTTTAAAAAGCAGCAGTCTCGCTGATACGCTGGCAGTTATTCTCTCCAATATGCATATGCACCAGATTCTTCTGGc                                                                                                                        <  1:503645/140‑1 (MQ=255)           aaTGGCTTCGGTTCTATTGCTAATATTTTGCTGATTATCGGGGCCGGAGGCGCATTCAACGCCATTTTAAAAAGCAGCAGTCTCGCTGATACGCTGGCAGTTATTCTCTCCAATATGCATATGCACCAGATTCTTCTGGc                                                                                                                        >  1:397825/1‑140 (MQ=255)             tGGCTTCGGTTCTATTGCTAATATTTTGCTGATTATCGGGGCCGGAGGCGCATTCAACGCCATTTTAAAAAGCAGCAGTCTCGCTGATACGCTGGCAGTTATTCTCTCCAATATGCATATGCACCAGATTCTTCTGGCCt                                                                                                                      >  2:649398/1‑140 (MQ=255)                cTTCGGTTCTATTGCTAATATTTTGCTGATTATCGGGGCCGGAGGCGCATTCAACGCCATTTTAAAAAGCAGCAGTCTCGCTGATACGCTGGCAGTTATTCTCTCCAATATGCATATGCACCAGATTCTTCTGGCCTGGt                                                                                                                   <  2:483022/140‑1 (MQ=255)                 ttCGGTTCTATTGCTAATATTTTGCTGATTATCGGGGCCGGAGGCGCATTCAACGCCATTTTAAAAAGCAGCAGTCTCGCTGATACGCTGGCAGTTATTCTCTCCAATATGCATATGCACCAGATTCTTCTGGCCTGGtt                                                                                                                  <  2:410535/140‑1 (MQ=255)                    ggTTCTATTGCTAATATTTTGCTGATTATCGGGGCCGGAGGCGCATTCAACGCCATTTTAAAAAGCAGCAGTCTCGCTGATACGCTGGCAGTTATTCTCTCCAATATGCATATGCACCAGATTCTTCTGGCCTGGTTAGt                                                                                                               <  2:161667/140‑1 (MQ=255)                                aaTATTTTGCTGATTATCGGGGCCGGAGGCGCATTCAACGCCATTTTAAAAAGCAGCAGTCTCGCTGATACGCTGGCAGTTATTCTCTCCAATATGCATATGCACCAGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCt                                                                                                   <  2:324664/140‑1 (MQ=255)                                aaTATTTTGCTGATTATCGGGGCCGGAGGCGCATTCAACGCCATTTTAAAAAGCAGCAGTCTCGCTGATACGCTGGCAGTTATTCTCTCCAATATGCATATGCACCAGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCt                                                                                                   >  2:647516/1‑140 (MQ=255)                                     tttGCTGATTATCGGGGCCGGAGGCGCATTCAACGCCATTTTAAAAAGCAGCAGTCTCGCTGATACGCTGGCAGTTATTCTCTCCAATATGCATATGCACCAGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATg                                                                                              >  2:333973/1‑140 (MQ=255)                                         cTGATTATCGGGGCCGGAGGCGCATTCAACGCCATTTTAAAAAGCAGCAGTCTCGCTGATACGCTGGCAGTTATTCTCTCCAATATGCATATGCACCAGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGc                                                                                          >  1:435055/1‑140 (MQ=255)                                           gATTATCGGGGCCGGAGGCGCATTCAACGCCATTTTAAAAAGCAGCAGTCTCGCTGATACGCTGGCAGTTATTCTCTCCAATATGCATATGCACCAGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAg                                                                                        <  2:240429/140‑1 (MQ=255)                                            aTTATCGGGGCCGGAGGCGCATTCAACGCCATTTTAAAAAGCAGCAGTCTCGCTGATACGCTGGCAGTTATTCTCTCCAATATGCATATGCACCAGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGt                                                                                       >  2:210871/1‑140 (MQ=255)                                             ttATCGGGGCCGGAGGCGCATTCAACGCCATTTTAAAAAGCAGCAGTCTCGCTGATACGCTGGCAGTTATTCTCTCCAATATGCATATGCACCAGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTg                                                                                      <  1:395388/140‑1 (MQ=255)                                              tATCGGGGCCGGAGGCGCATTCAACGCCATTTTAAAAAGCAGCAGTCTCGCTGATACGCTGGCAGTTATTCTCTCCAATATGCATATGCACCAGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTgg                                                                                     <  2:36717/140‑1 (MQ=255)                                               aTCGGGGCCGGAGGCGCATTCAACGCCATTTTAAAAAGCAGCAGTCTCGCTGATACGCTGGCAGTTATTCTCTCCAATATGCATATGCACCAGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTggg                                                                                    <  2:176870/140‑1 (MQ=255)                                                          aGGCGCATTCAACGCCATTTTAAAAAGCAGCAGTCTCGCTGATACGCTGGCAGTTATTCTCTCCAATATGCATATGCACCAGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCg                                                                         <  1:157241/140‑1 (MQ=255)                                                               cATTCAACGCCATTTTAAAAAGCAGCAGTCTCGCTGATACGCTGGCAGTTATTCTCTCCAATATGCATATGCACCAGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCa                                                                    >  1:564098/1‑140 (MQ=255)                                                               cATTCAACGCCATTTTAAAAAGCAGCAGTCTCGCTGATACGCTGGCAGTTATTCTCTCCAATATGCATATGCACCAGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCa                                                                    <  2:397825/140‑1 (MQ=255)                                                                       gCCATTTTAAAAAGCAGCAGTCTCGCTGATACGCTGGCAGTTATTCTCTCCAATATGCATATGCACCAGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGATggg                                                            >  1:542925/1‑140 (MQ=255)                                                                         cATTTTAAAAAGCAGCAGTCTCGCTGATACGCTGGCAGTTATTCTCTCCAATATGCATATGCACCAGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGATggggg                                                          >  1:697716/1‑140 (MQ=255)                                                                                 aaaGCAGCAGTCTCGCTGATACGCTGGCAGTTATTCTCTCCAATATGCATATGCACCAGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGATGGGGGCAACGGCa                                                  <  1:210871/140‑1 (MQ=255)                                                                                  aaGCAGCAGTCTCGCTGATACGCTGGCAGTTATTCTCTCCAATATGCATATGCACCAGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGATGGGGGCAACGGCaa                                                 <  1:177473/140‑1 (MQ=255)                                                                                     cagcagTCTCGCTGATACGCTGGCAGTTATTCTCTCCAATATGCATATGCACCAGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTACATGCGGCAGTGGGCTCCGCTACCGTGGCAatgat                                                               <  1:449968/123‑1 (MQ=255)                                                                                     cagcagTCTCGCTGATACGCTGGCAGTTATTCTCTCCAATATGCATATGCACCAGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTACATGCGGCAGTGGGCTCCGCTACCGTGGCAatgat                                                               >  2:449968/1‑123 (MQ=255)                                                                                        cagTCTCGCTGATACGCTGGCAGTTATTCTCTCCAATATGCATATGCACCAGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGATGGGGGCAACGGCAAttgttg                                           >  1:112480/1‑140 (MQ=255)                                                                                                      cGCTGGCAGTTATTCTCTCCAATATGCATATGCACCAGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGATGGGGGCAACGGCAATTGTTGCACCCATGCTGCCg                             <  2:542925/140‑1 (MQ=255)                                                                                                      cGCTGGCAGTTATTCTCTCCAATATGCATATGCACCAGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGATGGGGGCAACGGCAATTGTTGCACCCATGCTGCCg                             <  1:649398/140‑1 (MQ=255)                                                                                                             aGTTATTCTCTCCAATATGCATATGCACCAGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGATGGGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATc                      >  2:697217/1‑140 (MQ=255)                                                                                                             aGTTATTCTCTCCAATATGCATATGCACCAGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGATGGGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATc                      >  1:632643/1‑140 (MQ=255)                                                                                                                     tctcCAATATGCATATGCACCAGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGATGGGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGGCATc              >  2:448695/1‑140 (MQ=255)                                                                                                                     tctcCAATATGCATATGCACCAGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGATGGGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATc              >  1:173568/1‑140 (MQ=255)                                                                                                                       tcCAATATGCATATGCACCAGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGATGGGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAg            >  1:252649/1‑140 (MQ=255)                                                                                                                            tatgcatatgcaCCAGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGATGGGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCgg       <  2:632643/140‑1 (MQ=255)                                                                                                                                 atatgcaCCAGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGATGGGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAatt  >  2:541292/1‑140 (MQ=255)                                                                                                                                 atatgcaCCAGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGATGGGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAatt  >  2:188249/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                   ACATACGGAAAATGGCTTCGGTTCTATTGCTAATATTTTGCTGATTATCGGGGCCGGAGGCGCATTCAACGCCATTTTAAAAAGCAGCAGTCTCGCTGATACGCTGGCAGTTATTCTCTCCAATATGCATATGCACCCGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCTACCGTGGCAATGATGGGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGACATCAGCCCGGAAATT  >  NC_000913/2478734‑2479001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑