Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,594,589	G→T	A424E (GCG→GAG)	tmcA ←	tRNA(Met) cytidine acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,594,589	0	G	T	100.0%	101.0 / NA	30	A424E (GCG→GAG)	tmcA	tRNA(Met) cytidine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (13/17);  total (13/17)

CCCGCTGACGAGCCGGATGAACTGCTATCCGGCTGACCCGCCGTCCACGCAATGTCGCCGCCAGTGGATTGTTGCCGTGCGCCGCCAGCGACTGGGCCACCAGATTACCCCGCGGGCGACGAAAACCTGCCCATACCGCCTGACTGAGTTGTTGAGATAATCCACCCTCATCCACCAGCCACAGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGTAAATCCAGCGGCG  >  NC_000913/2594452‑2594718                                                                                                                                          |                                                                                                                                  cccGCTGACGAGCCGGATGAACTGCTATCCGGCTGACCCGCCGTCCACGCAATGTCGCCGCCAGTGGATTGTTGCCGTGCGCCGCCAGCGACTGGGCCACCAGATTACCCCGCGGGCGACGAAAACCTGCCCATACCTcc                                                                                                                                 >  1:24185/1‑140 (MQ=255)     cTGACGAGCCGGATGAACTGCTATCCGGCTGACCCGCCGTCCACGCAATGTCGCCGCCAGTGGATTGTTGCCGTGCGCCGCCAGCGACTGGGCCACCAGATTACCCCGCGGGCGACGAAAACCTGCCCATACCTCctgac                                                                                                                             >  1:328608/1‑140 (MQ=255)      tGACGAGCCGGATGAACTGCTATCCGGCTGACCCGCCGTCCACGCAATGTCGCCGCCAGTGGATTGTTGCCGTGCGCCGCCAGCGACTGGGCCACCAGATTACCCCGCGGGCGACGAAAACCTGCCCATACCTCctgact                                                                                                                            <  2:91163/140‑1 (MQ=255)        aCGAGCCGGATGAACTGCTATCCGGCTGACCCGCCGTCCACGCAATGTCGCCGCCAGTGGATTGTTGCCGTGCGCCGCCAGCGACTGGGCCACCAGATTACCCCGCGGGCGACGAAAACCTGCCCATACCTCctgactga                                                                                                                          <  1:685002/140‑1 (MQ=255)         cGAGCCGGATGAACTGCTATCCGGCTGACCCGCCGTCCACGCAATGTCGCCGCCAGTGGATTGTTGCCGTGCGCCGCCAGCGACTGGGCCACCAGATTACCCCGCGGGCGACGAAAACCTGCCCATACCTCCTGACTGAg                                                                                                                         <  2:641398/140‑1 (MQ=255)            gCCGGATGAACTGCTATCCGGCTGACCCGCCGTCCACGCAATGTCGCCGCCAGTGGATTGTTGCCGTGCGCCGCCAGCGACTGGGCCACCAGATTACCCCGCGGGCGACGAAAACCTGCCCATACCTCCTGACTGAgttg                                                                                                                      <  2:257169/140‑1 (MQ=255)                 aTGAACTGCTATCCGGCTGCCCCGCCGTCCACGCAATGTCGCCGCCAGTGGATTGTTGCCGTGCGCCGCCAGCGACTGGGCCACCAGATTACCCCGCGGGCGACGAAAACCTGCCCATACCTCCTGACTGAGTTGTTgag                                                                                                                 <  2:96972/140‑1 (MQ=255)                       tGCTATCCGGCTGACCCGCCGTCCACGCAATGTCGCCGCCAGTGGATTGTTGCCGTGCGCCGCCAGCGACTTGGCCACCAGATTACCCCGCGGGCGACGAAAACCTGCCCATACCTCCTGACTGAGTTGTTGAGATAATc                                                                                                           <  2:527738/140‑1 (MQ=255)                              cGGCTGACCCGCCGTCCACGCAATGTCGCCGCCAGTGGATTGTTGCCGTGCGCCGCCAGCGACTGGGCCACCAGATTACCCCGCGGGCGACGAAAACCTGCCCATACCTCCTGACTGAGTTGTTGAGATAATCCACCCTc                                                                                                    <  1:547492/140‑1 (MQ=255)                              cGGCTGACCCGCCGTCCACGCAATGTCGCCGCCAGTGGATTGTTGCCGTGCGCCGCCAGCGACTGGGCCACCAGATTACCCCGCGGGCGACGAAAACCTGCCCATACCTCCTGACTGAGTTGTTGAGATAATCCACCCTc                                                                                                    <  2:44212/140‑1 (MQ=255)                              cGGCTGACCCGCCGTCCACGCAATGTCGCCGCCAGTGGATTGTTGCCGTGCGCCGCCAGCGACTGGGCCACCAGATTACCCCGCGGGCGACGAAAACCTGCCCATACCTCCTGACTGAGTTGTTGAGATAATCCACCCTc                                                                                                    <  2:269330/140‑1 (MQ=255)                                   gACCCGCCGTCCACGCAATGTCGCCGCCAGTGGATTGTTGCCGTGCGCCGCCAGCGACTGGGCCACCAGATTACCCCGCGGGCGACGAAAACCTGCCCATACCTCCTGACTGAGTTGTTGAGATAATCCACCCTCATcca                                                                                               >  2:656199/1‑140 (MQ=255)                                              cACGCAATGTCGCCGCCAGTGGATTGTTGCCGTGCGCCGCCAGCGACTGGGCCACCAGATTACCCCGCGGGCGACGAAAACCTGCCCATACCTCCTGACTGAGTTGTTGAGATAATCCACCCTCATCCACCAGCCACAgc                                                                                    >  2:60760/1‑140 (MQ=255)                                                          ccgccAGTGGATTGTTGCCGTGCGCCGCCAGCGACTGGGCCACCAGATTACCCCGCGGGCGACGAAAACCTGCCCATACCTCCTGACTGAGTTGTTGAGATAATCCACCCTCATCCACCAGCCACAGCGCCCCGGCAAtc                                                                        <  1:6764/140‑1 (MQ=255)                                                           cgccAGTGGATTGTTGCCGTGCGCCGCCAGCGACTGGGCCACCAGATTACCCCGCGGGCGACGAAAACCTGCCCATACCTCCTGACTGAGTTGTTGAGATAATCCACCCTCATCCACCAGCCACAGCGCCCCGGCAAtct                                                                       >  1:17175/1‑140 (MQ=255)                                                                 tGGATTGTTGCCGTGCGCCGCCAGCGACTGGGCCACCAGATTACCCCGCGGGCGACGAAAACCTGCCCATACCTCCTGACTGAGTTGTTGAGATAATCCACCCTCATCCACCAGCCACAGCGCCCCGGCAATCTCGtttt                                                                 >  2:135097/1‑140 (MQ=255)                                                                        ttgCCGTGCGCCGCCAGCGACTGGGCCACCAGATTACCCCGCGGGCGACGAAAACCTGCCCATACCTCCTGACTGAGTTGTTGAGATAATCCACCCTCATCCACCAGCCACAGCGCCCCGGCAATCTCGTTTTCGCCAgc                                                          <  1:363257/140‑1 (MQ=255)                                                                              tGCGCCGCCAGCGACTGGGCCACCAGATTACCCCGCGGGCGACGAAAACCTGCCCATACCTCCTGACTGAGTTGTTGAGATAATCCACCCTCATCCACCAGCCACAGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTg                                                    >  1:512222/1‑140 (MQ=255)                                                                                  ccgccAGCGACTGGGCCACCAGATTACCCCGCGGGCGACGAAAACCTGCCCATACCTCCTGACTGAGTTGTTGAGATAATCCACCCTCATCCACCAGCCACAGCGCCCCGGCAATCTCGTTTTCGCCAgccgcc                                                      <  1:318382/134‑1 (MQ=255)                                                                                  ccgccAGCGACTGGGCCACCAGATTACCCCGCGGGCGACGAAAACCTGCCCATACCTCCTGACTGAGTTGTTGAGATAATCCACCCTCATCCACCAGCCACAGCGCCCCGGCAATCTCGTTTTCGCCAgccgcc                                                      >  2:318382/1‑134 (MQ=255)                                                                                  ccgccAGCGACTGGGCCACCAGATTACCCCGCGGGCGACGAAAACCTGCCCATACCTCCTGACTGAGTTGTTGAGATAATCCACCCTCATCCACCAGCCACAGCGCCCCGGCAATCTCGTTTTCGCCAgccgcc                                                      >  2:592710/1‑134 (MQ=255)                                                                                  ccgccAGCGACTGGGCCACCAGATTACCCCGCGGGCGACGAAAACCTGCCCATACCTCCTGACTGAGTTGTTGAGATAATCCACCCTCATCCACCAGCCACAGCGCCCCGGCAATCTCGTTTTCGCCAgccgcc                                                      <  1:592710/134‑1 (MQ=255)                                                                                       aGCGACTGGTCCACCATATTCCCCCGCGGGCGACGAAAACCTGCCCATACCTCCTGACTGAGTTGTTGAGATAACCCCCCCTCACCCGCCAGCCCCAGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATg                                           <  2:17175/140‑1 (MQ=255)                                                                                       aGCGACTGGGCCACCAGATTACCCCGCGGGCGACGAAAACCTGCCCATACCTCCTGACTGAGTTGTTGAGATAATCCACCCTCATCCACCAGCCACAGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATg                                           <  1:121686/140‑1 (MQ=255)                                                                                           aCTGGGCCACCAGATTACCCCGCGGGCGACGAAAACCTGCCCATACCTCCTGACTGAGTTGTTGAGATAATCCACCCTCATCCACCAGCCACAGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGc                                       >  2:338794/1‑140 (MQ=255)                                                                                               ggCCACCAGATTACCCCGCGGGCGACGAAAACCTGCCCATACCTCCGGACTGAGTTGTTGAGATAATCCCCCCTCATCCACCAGCCACAGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTg                                   <  2:177161/140‑1 (MQ=255)                                                                                                              ccGCGGGCGACGAAAACCTGCCCATACCTCCTGACTGAGTTGTTGAGATAATCCACCCTCATCCACCAGCCACAGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATcc                    >  2:31246/1‑140 (MQ=255)                                                                                                                 cgGGCGACGAAAACCTGCCCATACCTCCTGACTGAGTTGTTGAGATAATCCACCCTCATCCACCAGCCACAGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGc                 >  1:213675/1‑140 (MQ=255)                                                                                                                      gacgaAAACCTGCCCATACCTCCTGACTGAGTTGTTGAGATAATCCACCCTCATCCACCAGCCACAGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGTaaa            >  1:716188/1‑140 (MQ=255)                                                                                                                                tGCCCATCCCTCCTGACTGAGTTGTTGAGATAATCCCCCCTCACCCACCAGCCACAGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGTAAATCCAgcggcg  <  2:328608/140‑1 (MQ=255)                                                                                                                                tGCCCATACCTCCTGACTGAGTTGTTGAGATAATCCCCCCTCATCCACCAGCCACAGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGTAAATCCAgcggcg  <  1:85721/140‑1 (MQ=255)                                                                                                                                tGCCCATACCTCCTGACTGAGTTGTTGAGATAATCCACCCTCATCCACCAGCCACAGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGTAAATCCAgcggcg  <  1:610432/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                  CCCGCTGACGAGCCGGATGAACTGCTATCCGGCTGACCCGCCGTCCACGCAATGTCGCCGCCAGTGGATTGTTGCCGTGCGCCGCCAGCGACTGGGCCACCAGATTACCCCGCGGGCGACGAAAACCTGCCCATACCGCCTGACTGAGTTGTTGAGATAATCCACCCTCATCCACCAGCCACAGCGCCCCGGCAATCTCGTTTTCGCCAGCCGCCTGTAAAAAATGTTGCCCTGGTGCATCCATCATCCGGCGTAAATCCAGCGGCG  >  NC_000913/2594452‑2594718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑