Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	187,926	G→T	A242E (GCA→GAA)	glnD ←	PII uridylyltransferase/uridylyl removing enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	187,926	0	G	T	100.0%	132.8 / NA	39	A242E (GCA→GAA)	glnD	PII uridylyltransferase/uridylyl removing enzyme
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (18/21);  total (18/21)

TCGTAACGGCTGACGACCAGATGCAGGGCAAAGCGAATACGCCACAATATATGCAGACATTCGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTGCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATCGCGCAAGCCGCCAGGGCTGCTTTTGATGTCTGGTTCAAGGTTGTAGCTGGTGCCATGGTAACGCTGATGGCGCTGGTTC  >  NC_000913/187800‑188048                                                                                                                               |                                                                                                                           tCGTAACGGCTGACGACCAGATGCAGGGCAAAGCGAATACGCCACAATATATGCAGACATTCGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTTCGCCAAAATGAcg                                                                                                               <  2:45004/140‑1 (MQ=255) tCGTAACGGCTGACGACCAGATGCAGGGCAAAGCGAATACGCCACAATATATGCAGACATTCGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTTCGCCAAAATGAcg                                                                                                               <  1:133343/140‑1 (MQ=255)    tAACGGCTGACGACCAGATGCAGGGCAAAGCGAATACGCCACAATATATGCAGACATTCGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTTCGCCAAAATGAcggcg                                                                                                            <  1:368212/140‑1 (MQ=255)      aCGGCTGACGACCAGATGCAGGGCAAAGCGAATACGCCACAATATATGCAGACATTCGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTTCGCCAAAATGACGGCggg                                                                                                          <  2:505903/140‑1 (MQ=255)           tGACGACCAGATGCAGGGCAAAGCGAATACGCCACAATATATGCAGACATTCGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTTCGCCAAAATGACGGCGGGCCAcc                                                                                                     >  1:446747/1‑140 (MQ=255)               gacCAGATGCAGGGCAAAGCGAATACGCCACAATATATGCAGACATTCGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTTCGCCAAAATGACGGCGGGCCACCCAtt                                                                                                 <  1:700790/140‑1 (MQ=255)                acCAGATGCAGGGCAAAGCGAATACGCCACAATATATGCAGACATTCGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTTCGCCAAAATGACGGCGGGCCACCCATTg                                                                                                <  1:124185/140‑1 (MQ=255)                  cAGATGCAGGGCAAAGCGAATACGCCACAATATATGCAGACATTCGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTTCGCCAAAATGACGGCGGGCCACCCATTGCa                                                                                              <  1:615926/140‑1 (MQ=255)                  cAGATGCAGGGCAAAGCGAATACGCCACAATATATGCAGACATTCGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTTCGCCAAAATGACGGCGGGCCACCCATTGCa                                                                                              <  2:63857/140‑1 (MQ=255)                   aGATGCAGGGCAAAGCGAATACGCCACAATATATGCAGACATTCGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTTCGCCAAAATGACGGCGGGCCACCCATTGc                                                                                               <  2:699915/138‑1 (MQ=255)                   aGATGCAGGGCAAAGCGAATACGCCACAATATATGCAGACATTCGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTTCGCCAAAATGACGGCGGGCCACCCATTGc                                                                                               >  1:699915/1‑138 (MQ=255)                       gCAGGGCAAAGCGAATACGCCACAATATATGCAGACATTCGTTTAATTCCGCCCGCTCCGCTGAGGATAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTTCGCCAAAATGACGGCGGGCCACCCATTGCCGAgtg                                                                                         >  1:125981/1‑140 (MQ=255)                          gggCAAAGCGAATACGCCACAATATATGCAGACATTCGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTTCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTgg                                                                                      >  1:403697/1‑140 (MQ=255)                           ggCAAAGCGAATACGCCACAATATATGCAGACATTCGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTTCGCCAAAATGACGGCGGGCCAccc                                                                                                    <  2:226272/125‑1 (MQ=255)                           ggCAAAGCGAATACGCCACAATATATGCAGACATTCGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTTCGCCAAAATGACGGCGGGCCAccc                                                                                                    >  1:226272/1‑125 (MQ=255)                               aaGCGAATACGGCACAATATATGCAGACATTCGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTTCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATc                                                                                 >  1:359142/1‑140 (MQ=255)                                aGCGAATACGCCACAATATATGCAGACATTCGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTTCGCCAAAATGACGGCGGCCCACCCATTGCAGAGTGTTGATATct                                                                                >  2:375207/1‑139 (MQ=255)                                  cGAATACGCCACAATATATGCAGACATTCGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTTCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATcgcg                                                                              <  2:454627/140‑1 (MQ=255)                                  cGAATACGCCACAATATATGCAGACATTCGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTTCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATcgcg                                                                              <  2:316594/140‑1 (MQ=255)                                              aaTATATGCAGACATTCGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTTCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATCGCGCAAGCCGCCAgg                                                                  <  2:403697/140‑1 (MQ=255)                                                tatatGCAGACATTCGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTTCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATCGCGCAAGCCGCCAGGgc                                                                <  1:440602/140‑1 (MQ=255)                                                        gACATTCGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTTCGCCAAAATGACGGTGGGCCACCCATTGCAGAGTGTGGATATCGCGCAAGCCGCCAGGGCTGCTTTTg                                                        >  2:501906/1‑140 (MQ=255)                                                        gACATTCGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTTCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATCGCGCAAGCCGCCAGGGCTGCTTTTg                                                        >  2:702494/1‑140 (MQ=255)                                                        gACATTCGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTTCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATCGCGCAAGCCGCCAGGGCTGCTTTTg                                                        <  2:278122/140‑1 (MQ=255)                                                        gACATTCGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTTCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATCGCGCAAGCCGCCAGGGCTGCTTTTg                                                        >  1:278122/1‑140 (MQ=255)                                                          cATTCGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTTCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATCGCGCAAGCCGCCAGGGCTGCTTTTGAt                                                      >  2:384352/1‑140 (MQ=255)                                                          cATTCGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTTCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATCGCGCAAGCCGCCAGGGCTGCTTTTGAt                                                      >  2:299301/1‑140 (MQ=255)                                                          cATTCGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTTCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATCGCGCAAGCCGCCAGGGCTGCTTTTGAt                                                      >  1:53118/1‑140 (MQ=255)                                                          cATTCGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTTCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATCGCGCAAGCCGCCAGGGCTGCTTTTGAt                                                      >  2:714781/1‑140 (MQ=255)                                                              cGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTTCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATCGCGCAAGCCGCCAGGGCTGCTTTTGATGTCt                                                  >  2:52917/1‑140 (MQ=255)                                                              cGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTTCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATCGCGCAAGCCGCCAGGGCTGCTTTTGATGTCt                                                  >  2:76812/1‑140 (MQ=255)                                                                  tAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTTCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATCGCGCAAGCCGCCAGGGCTGCTTTTGATGTCTGGtt                                              <  2:446747/140‑1 (MQ=255)                                                                         gcccgcTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTTCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATCGCGCAAGCCGCCAGGGCTGCTTTTGATGTCTGGTTCAAGGtt                                       <  1:299301/140‑1 (MQ=255)                                                                          cccgcTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTTCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATCGCGCAAGCCGCCAGGGCTGCTTTTGATGTCTGGTTCAAGGTTg                                      >  2:549862/1‑140 (MQ=255)                                                                                      aGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTTCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATCGCGCAAGCCGCCAGGGCTGCTTTTGATGTCTGGTTCAAGGTTGTAGCTGGTGCCa                          <  1:549862/140‑1 (MQ=255)                                                                                      aGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTTCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATCGCGCAAGCCGCCAGGGCTGCTTTTGATGTCTGGTTCAAGGTTGTAGCTGGTGCCa                          <  1:375207/140‑1 (MQ=255)                                                                                                   aaaCCCGACCATTTCATCCAGCGATGTTTCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATCGCGCAAGCCGCCAGGGCTGCTTTTGATGTCTGGTTCAAGGTTGTAGCTGGTGc                            <  2:393838/125‑1 (MQ=255)                                                                                                   aaaCCCGACCATTTCATCCAGCGATGTTTCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATCGCGCAAGCCGCCAGGGCTGCTTTTGATGTCTGGTTCAAGGTTGTAGCTGGTGc                            >  1:393838/1‑125 (MQ=255)                                                                                                        cGACCATTTCATCCAGCGATGTTTCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATCGCGCAAGCCGCCAGGGCTGCTTTTGATGTCTGGTTCAAGGTTGTAGCTGGTGCCATGGTAACGCTGATGgcgc        <  2:608766/140‑1 (MQ=255)                                                                                                              tttCATCCAGCGATGTTTCGCCAAAATGACGGCGGGCCACCCATTGCAGAGCGTGGATATCGCGCAAGCCGCCAGGGCTGCTTTTGATGTCTGGTTCAAGGTTGTAGCTGGTGCCATGGTAACGCTGATGGCGCTGgttc  >  1:295863/1‑140 (MQ=255)                                                                                                                               |                                                                                                                           TCGTAACGGCTGACGACCAGATGCAGGGCAAAGCGAATACGCCACAATATATGCAGACATTCGTTTAATTCCGCCCGCTCCGCTGAGGTTAAGAAGCCAAACCCGACCATTTCATCCAGCGATGTTGCGCCAAAATGACGGCGGGCCACCCATTGCAGAGTGTGGATATCGCGCAAGCCGCCAGGGCTGCTTTTGATGTCTGGTTCAAGGTTGTAGCTGGTGCCATGGTAACGCTGATGGCGCTGGTTC  >  NC_000913/187800‑188048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑